NA ----> NA

Intron 1 GTGCAAAGGT ... GGCAATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	89.35	9	54	97
A2	98.18	1	29	79
A3	89.8	10	60	116
A4	87.05	9	38	83
A5	89.29	3	16	34
A6	100	0	3	5
A7	100	0	4	1
A8	100	0	3	2
A9	-1	0	0	0
A10	100	0	4	2

>TRINITY_DN111431_c1_g1_i7 from sample A5 / Example spliced isoform

GGGAAGGCCAAGGAAGATGAGTGCAAGTGCTGAAATGTCTTTGTGTGAGAGAGATATGGGTTGCTCAGTAAACATGGGCCATCAGGTGCGAAGGTTGAGGTAGATGATGAGTTCTACATTAGTTGATATCAACCAATTTTGCAGGTTTAGGAATGTTGCATCTTTACAGGTACAATGGATGAGGAGAAGGCTAAGGAGTA
GGAGGTATGCATTGTTTTATGGCAGTTTAACTTAACTCATTTGTTGTTGCATCATTATAT

>TRINITY_DN111431_c1_g1_i10 from sample A5 / Example unspliced isoform:

GGAATGATGTAGATTGCACATTGCAATGGGTGAATGGGAAGGCCAAGGAAGATGAGgtgcaaaggttgagaccgagggtgaattctacatttgtcgacaacaatccattttgtgggtatagtttatgcatatttactaatacaatgggcaatgagaagggtaaggagtaagagatataaagtgttttatgcaagttgaac
ttaaagtatttttttgatacatcattatatcatgtgtttgtttaaatttggaggcaatgtagTGCAAGTGCTGAAATGTCTTTGTGTGAGAGAGATATGGGTTGCTCAGTAAACATGGGCCATCAGGT

Intron 2 GTATGGATTT ... TGGTGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.78	72	84	130
A2	54.18	63	65	84
A3	44.97	104	87	83
A4	47.13	69	59	64
A5	49.09	28	28	26
A6	43.59	11	10	7
A7	40	6	3	5
A8	60	2	2	4
A9	33.33	1	0	1
A10	100	0	2	0

>TRINITY_DN45143_c1_g5_i1 from sample A6 / Example spliced isoform

TACAATGGGCAATGAGAAGGATATGGGTTGCTCAGTAAACATGGGCCATCAGGTGCGAAGGTTGAGGTAGATGGTGAGTTCTACATTAGTTGATATCAACCAATTTTGCAGGTTTAGGAATGTTGCATCTTTACAGGTACAATGGATGGGTAGAAGGCTAAGGAGTAGGAGGTTGTCATTTGCTTGCCATGGAAGCATTC
AGGGGTTGTAGGGTGGAATTTGTGTTTGTTATTTCTCTTTCTTTGTTTTTTTTATCTCTTTTTTGCTGCTCTTTTTTGTTGTTTTTGTAACGTTTGCTTTGTTGTATTTTTCATTTTTCTATTGCTTTTAAGTGTTTTTTTTTTGCATTTTTTAGCAT

>TRINITY_DN45143_c1_g5_i2 from sample A6 / Example unspliced isoform:

CTCTAATGACACCGTCGAAATATGCTTGTGTGTAGGTGCGAAGGTTGAGGTAGATGGTGAGTTCTACATTAGTTGATATCAACCAATTTTGCAGGTTTAGGAATGTTGCATCTTTACAGGTACAATGGATGGGTAGAAGGCTAAGGAGTAGGAGgtatggatttaagtactaagccagttgaacttggttgataagtttt
ggtgtttgcataagatgtacttgtgcataagattactcatgtaagattttctcataagatatttggttagtgcttaaagtttataataagattttctggtatctatttaatgcttaacaaaactttggtgtgtagGTTGTCATTTGCTTGCCATGGAAGCATTCAGGGGTTGTAGGGTGGAATTTGTGTTTGTTATTTCT
CTTTCTTTGTTTTTTTTATCTCTTTTTTGCTGCTCTTTTTTGTTGTTTTTGTAACGTTTGCTTTGTTGTATTTTTCATTTTTCTATTGCTTTTAAGTGTTTTTTTTTTGCATTTTTTAGCAT

Intron 3 GTGCAAAGGT ... GGCAATGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	87.23	9	64	59
A2	98	1	32	66
A3	88.17	10	64	85
A4	86.96	9	44	76
A5	86.96	3	18	22
A6	100	0	3	0
A7	100	0	10	3
A8	100	0	13	1
A9	-1	0	0	0
A10	100	0	8	1

>TRINITY_DN111431_c1_g1_i7 from sample A5 / Example spliced isoform

GGGAAGGCCAAGGAAGATGAGTGCAAGTGCTGAAATGTCTTTGTGTGAGAGAGATATGGGTTGCTCAGTAAACATGGGCCATCAGGTGCGAAGGTTGAGGTAGATGATGAGTTCTACATTAGTTGATATCAACCAATTTTGCAGGTTTAGGAATGTTGCATCTTTACAGGTACAATGGATGAGGAGAAGGCTAAGGAGTA
GGAGGTATGCATTGTTTTATGGCAGTTTAACTTAACTCATTTGTTGTTGCATCATTATAT

>TRINITY_DN111431_c1_g1_i2 from sample A5 / Example unspliced isoform:

GGAATGATGTAGATTGCACATTGCAATGGGTGAATGGGAAGGCCAAGGAAGATGAGgtgcaaaggttgagaccgagggtgaattctacatttgtcgacaacaatccattttgtgggtatagggtttatgcatatttactaatacaatgggcaatgagaagggtaaggagtaagagatatgcattgttctatgctagttga
acttaagtctttttttgatacatcattatatcatgtgtttgtttaaatttggaggcaatgtagTGCAAGTGCTGAAATGTCTTTGTGTGAGAGAG