NA ----> NA

Intron 1 GTATTATTTT ... ATATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	92.34	41	372	616
A2	89.58	35	214	388
A3	92.59	22	207	343
A4	94.27	19	240	385
A5	94.33	10	131	202
A6	89.23	42	247	449
A7	88.02	90	496	826
A8	86.79	74	372	600
A9	87.61	55	302	476
A10	90.08	62	444	682

>TRINITY_DN118083_c0_g1_i3 from sample A3 / Example spliced isoform

CCACACCAAAGTGGCTATTATCCTAGGGAGGAATATGAGAGTCCATCCTTCACAGAAATCAGACAGAGGGTAAATATGTGTCTGTTTGAAACATTGGATTGTCCACAATCTACAGCCGTGTCACAGTCGTCCACAATATGCAGCAGACTAATATACGTTTGAAGCATTGTTTTGAAATCAATAATCCGCAAGTGGGGTAA
TTTTCGGCTCCAAAAGAACATTCAAGAGGGATTATGAGAATCCATCCATTACAGAAATCACAGAGGGGATAAAGATGTATATCTGTTTGAGCCTTTGGGTTGTCCACAATCTACGGCCATATCCGCCGCC

>TRINITY_DN118083_c0_g1_i4 from sample A3 / Example unspliced isoform:

GTTCGTTTATCTGAAGTAGATCTGAAAGAGGGGATTTGACAAACTTGCAAGCACACCACACCAAAGTGGCTATTATCCTAGGGAgtattattttaatcatgtagtctcttcaatatcggttaattttttttatttaggtgattatacttgtcctatatattggcagGGAATATGAGAGTCCATCCTTCACAGAAATCAGA
CAGAGGGTAAATATGTGTCTGTTTGAAACATTGGATTGTCCACAATCTACAGCCGTGTCACAGTCGTCCACAATATGCAGCAGACTAATATACGTTTGAAGCATTGTTTTGAAATCAATAATCCGCAAGTGGGGTAATTTTCGGCTCCAAAAGAACATTCCAAGAGGTAAGAGGTTTGTTGTTAAGTGTTTCTTAAGTTC
TCTGAAATCACAGATACTGTCCTGTGCTCTTGTACACACTACACAGTGGTGATTGTTTTGAAATCAATAGTCCACAAGTGGGCTAATTTTCGGCTCCAAAAGATCATTCAAGATGTTTGTTAAGTTCTCTAAAATCACAGATACTGTCCTGCGCTCTTGTACACACTACACATTTGTGATTTATAGGTGTAAGCTT

Intron 2 GTTTGTTGTT ... ATATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	68.69	98	114	315
A2	72.72	47.5	64	187
A3	69.72	62	74	210
A4	70.78	55	88	178
A5	72.4	34.5	52	129
A6	61.65	102.5	92	237
A7	60.6	179.5	162	387
A8	58.77	154.5	132	306
A9	56.18	131	91	243
A10	55.23	169.5	108	309

>TRINITY_DN47365_c1_g1_i18 from sample A10 / Example spliced isoform

CGTTGTTTTGAAATCAATAATCCTCACATCATTCAAGAGGTGTGGGGTAATTTTCGGCTCCAAAAGATCATTCAAGAGGGATTATGAGAATCCGTCCATTACAGAAATCACAGAGGGGTAAATATGTATTTTCTGTTTGAGCCTTTGGGTTGTCCACAATCTACGGCCATATCCGCCGTCCAAAATCTGCAGCACAGTAA
CGTACTTATTGTTTTGTAAGCGATATTCTGCAAGTGCGGTAATTTTCGGTTTGAAAACATCATTCAAGAGGCTTGAGTAGTGGG

>TRINITY_DN47365_c1_g1_i14 from sample A10 / Example unspliced isoform:

TTGTTGTTAAGTGTTTCTTAAGTTCTCTGAAATCACAGATACTGTCCTGTGGGGTAATTTTCGGCTCCAAAAGATCATTCAAGAGgtttgttgttaagtgtttcttaagttctctaaaatcacagatactgtcctgtgctattgtacacagtacacagtacacagttgtgatttataggtgtaagcttgcatgcccccta
caccaaagtggctattatcctaggaagtacaatttgaatcatatagtctctgcaataccagtttatgtttatttgattttattttggtggttatacttgtcctatattggcagGGATTATGAGAATCCATCCATTACAGAAATCACAGAGGGGTAAATATGTATTTTCTGTTTGAGCCTTTGGGTTGTCCACAATCTACG
GCCATATCCGCCGTCCAAAATCTGCAGCACAGTAACGTACTTATTGTTTTGTAAGCGATATTCTGCAAGTGCGGTAATTTTCGGTTTGAAAACATCATTCAAGAGGCTTGAGTAGTGGG

Intron 3 GTATTATTTT ... ATATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.09	79	369	609
A2	77.38	88	213	389
A3	82.01	60	204	343
A4	79.15	83	239	391
A5	82.93	35	131	209
A6	74.06	121	244	447
A7	73.38	238	477	835
A8	77.56	135	361	572
A9	70.67	161	298	478
A10	78.37	151	436	658

>TRINITY_DN103693_c0_g1_i9 from sample A4 / Example spliced isoform

GTTCGTTTATCTGAAGTGGATCTGAAAGAGGGGATTTGACAAACTTGCAAGCACACCACACCAAAGTGGCTATTATCCTAGGGAGGAATATGACCATCCTTCACAGAAATCAGACAAGAGGGTAAATATCTGTCTGTTTGAAACATTGGGTTGTCCACAATCGACAGCCGTGTCAGTCGTCCACAATCTGCAGCAGCAGA
CTAATATACGTTTGAAGCGTTGTTTTGAAATCAATAATCCACAAGTGGGGTAATTTTCGGCTCGGCTCCAAAAGATCATTCAAGAGGTTTGTTGTTAGGTGTTTCTTAAGTTCTCTAAAATCACAGATACTGTCCTGTGCTCTTGTACACACTACACAGTACACAGTTGTGATTTATAGGTGTAAGCTTGCATGCCCCCC
ACACCAAAGTGGCTATTATCCTAGGGAGGGTGATTGTTTTGAAATCAATAATCCACAAGTGGGGTAATTTTCGGCTCCAAAAGATCTTTCAAGAGGTTTATAGGGGTAATTTTTCTTTAGTTCTCTAAAATCACAGATACTGTCCTGTG

>TRINITY_DN103693_c0_g1_i4 from sample A4 / Example unspliced isoform:

GTTCGTTTATCTGAAGTGGATCTGAAAGAGGGGATTTGACAAACTTGCAAGCACACCACACCAAAGTGGCTATTATCCTAGGGAgtattattttaatcatgtagtctcttcaatatcggttaatttttttattcaggtgattatacttgtcctatatattggcagGGAATATGACCATCCTTCACAGAAATCAGACAAGA
GGGTAAATATCTGTCTGTTTGAAACATTGGGTTGTCCACAATCGACAGCCGTGTCAGTCGTCCACAATCTGCAGCAGCAGACTAATATACGTTTGAAGCGTTGTTTTGAAATCAATAATCCACAAGTGGGGTAATTTTCGGCTCGGCTCCAAAAGATCATTCAAGAGGTTTGTTGTTAGGTGTTTCTTAAGTTCTCTAAA
ATCACAGATACTGTCCTGTGCTCTTGTACACACTACACAGTACACAGTTGTGATTTATAGGTGTAAGCTTGCATGCCCCCCACACCAAAGTGGCTATTATCCTAGGGAGGGTGATTGTTTTGAAATCAATAATCCACAAGTGGGGTAATTTTCGGCTCCAAAAGATCTTTCAAGAGGTTTATAGGGGTAATTTTTCTTTA
GTTCTCTAAAATCACAGATACTGTCCTGTG

Intron 4 GTTTATAGGG ... ATATTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.33	98	1	269
A2	77.88	47.5	1	166
A3	73.93	62	0	175
A4	73.2	55	0	150
A5	75.95	34.5	0	109
A6	67.84	102.5	1	216
A7	66.71	179.5	2	358
A8	64.47	154.5	1	279
A9	62.14	131	1	214
A10	61.58	169.5	2	271

>TRINITY_DN47365_c1_g1_i18 from sample A10 / Example spliced isoform

CGTTGTTTTGAAATCAATAATCCTCACATCATTCAAGAGGTGTGGGGTAATTTTCGGCTCCAAAAGATCATTCAAGAGGGATTATGAGAATCCGTCCATTACAGAAATCACAGAGGGGTAAATATGTATTTTCTGTTTGAGCCTTTGGGTTGTCCACAATCTACGGCCATATCCGCCGTCCAAAATCTGCAGCACAGTAA
CGTACTTATTGTTTTGTAAGCGATATTCTGCAAGTGCGGTAATTTTCGGTTTGAAAACATCATTCAAGAGGCTTGAGTAGTGGG

>TRINITY_DN47365_c1_g1_i11 from sample A10 / Example unspliced isoform:

AAGTGGGGTAATTTTCGGCTCCAAAAGATCATTCAAGAGgtttataggggtaatttttcttttccaaaagatcattcaagaggtttgttgttaagtgtttcttaagttctctaaaatcacagatactgttctgcgctcttgtacacattacacagtacacagttgtgatttataggtctaagcttgcatgccccccacac
caaagtggctattatcctaggaagtacaatttgaatcatatagtctctgcaatacgggtttatgttttttgaatttatttaggtggttatacttgtcctatattggcaggatattggcagGGATTATGAGAATCCATCCATTACAGAAATCACAGAGGGGTAAATATGTATTTTCTGTTTGAGCCTTTGGGTTGTCCACA
ATCTACGGCCATATCCGCCGTCCAAAATCTGCAGCACAGTAACGTACTTATTGTTTTGTAAGCGATATTCTGCAAGTGCGGTAATTTTCGGTTTGAAAACATCATTCAAGAGGCTTGAGTAGTGGG

Intron 5 GTAGTTCTCT ... CATTTAAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.4	208	6	220
A2	52.76	154	7	172
A3	52.06	128	7	139
A4	52.73	156	2	174
A5	51.74	83	1	89
A6	46.19	212	12	182
A7	49.93	344	9	343
A8	50.11	236	4	237
A9	42.47	233	3	172
A10	45.83	279	3	236

>TRINITY_DN47365_c1_g1_i2 from sample A10 / Example spliced isoform

CCCCCCACACCAAAGTGGCTATTATCCTAGGGAGTATTATTTTAATCATGTAGTCTCTTCAATATCGGTTAATTTTTTTTATTTAGGTGATTATACTTGTCCTATATATTGGCAGGCAATATGAGAGTCCATCCTTCACAGAAATCAGACAAGAGGGTAAATATGTGTCTGTTTGAAACATTGGGCTGTCCACAATCTAC
AGCCGTGTCAGTCGTCCACAATCTGCTGCAGACTAATATACGTTTGAAGCGTTGTTTTGAAATCAATAATCCACAAGTGGGGTAATTTTCGGCTCCAAAAGATCATTCAAGAGGTTTGTTGTTAAGTGTTTCTTAAGTTCTCTGAAATCACAGATACTGTCCTGTGAAGTGGGGTAATTTTCGGCTCCAAAAGATCATTC
AAGAGGTTTGTTGTTAAGTGTTTCTTAAGTTCTC

>TRINITY_DN47365_c1_g1_i1 from sample A10 / Example unspliced isoform:

CCCCCCACACCAAAGTGGCTATTATCCTAGGGAGTATTATTTTAATCATGTAGTCTCTTCAATATCGGTTATTTTTTTTATTTGGGTGATTATACTTGGTCCTATATATTGGCAGGGAATATGAGAGTCCATCCTTCACAGAAATCAGACAAGAGGGTAAATATCTGTCTGTTTGAAACATTGGGTTGTCCACAATCTAC
AGCCTTAAGTGGGGTAATTTTCGGCTCCAAAAGATCATTCAAGAGgtagttctctgaaatcacagatactgtcctgtgtgtacacactactacatagtgattgttttgaaatcaataatccacaagtggggtaattttcggctccaaaagatcatttaagagGTTTGTTGTTAAGTGTTTCTTAAGTTCTCTAAAATCAC
AGATACTGTCCTGTGCTCTTGTACACATTACACAGTACACAGTTGTGATTTATAGGTGTAAGCTTGCATGCCCCCCACACCAAAGTGACTATTATCCTAGGAAGTACAATTTGGATCATAATACCGGTTTATGTTTTTTTATTTTATTTAGGTGGTAAAAGAATCCATCCATTACAGAAATCACAGAGGGGTAAAATGTA
TATCTGTTTAAGCCTTTGGGTTGTTCACAATCTACAGCCATGTCCACCGTCCAAAATCTGTAGCCATATCCGCCGTCCAAAATCTGCAGCACACTAACACACTTACTGTTTCGTAAGCGATATTCTGCAAGTGCGGTAATTTTCGGTTCCAAAACATCATTCAAGAGGTTTGTTGTTTTCTAAAGTCACATATACTCCAC
AATCTACGGCCATATCCGCCGTCCAAAATCTGCAGCACACTAACACACTTAGTAACGTACTTATTGTTTTGTAAGCGATATTCTGCAAGTGCGGTAATTTTCGGTTTGAAAACATCATTCAAGAGGCTTGAGTAGTGGG