sp|Q40864|EML_PICGL ----> Em-like protein

Intron 1 GTACTGATTG ... TAAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.78	709	186	79.7
A2	16.19	959.9	269.1	101.6
A3	15.34	1133.6	298.3	112.6
A4	16.99	738.7	225.4	77
A5	13.73	733.9	176.9	56.7
A6	16.55	14990.1	4326.4	1618.6
A7	16.24	10220.1	2987.9	975
A8	15.95	8043.4	2156.9	896.7
A9	15.98	7448.1	2101.4	732
A10	15.53	8071.9	2248.9	720.1

>TRINITY_DN121050_c4_g4_i1 from sample A1 / Example spliced isoform

CTTGAAGTTGAAGTTAATGTTACTGTTTGGTATTTCTTGTTGAAGTTACTGCGTTTTCTTTTGTTTATTCAGAGAAGAGTGATTGTTATAAGTAAAGATGGCACATAGGCCAGTTACGAGAGAGCTGGATGCCAAGGCGCGGGAGGGAGAGACTATCGTGCCCGGTGGCACCGGTGGGAAGTCCGTTGATGCCCAAGAGC
GTCTTGCTGAAGGGCGGAGCAGAGGTGGCCAGACTCGAAAGGAACAAATTGGCAGCGAAGGATACCAGGAGATGGGCCGCAAGGGAGGGCTTACCACACAAGACAAGTCCGGTGCAGAACGGGCAGCTGAAGAGGGTATCCCCATTGATGAGTCCAAATACATGACCAATTGATTCCAGCTGTTACCGTTTTCCTGCAAT
ATATATACACATGGATATATATTGTATATATCCTCTGTGTTCCAACTTCCGCCAACAGATTTCTTCTTCTTTCGAAATTTCTCTATTGTCATATAGTTATCGTTTCTTTGTGTTACATGTTAGTTTGATTTCAATAACCATTCGTTGGTTTCCTCTCCTTTTTTAATGAAATAAATCTTCTTTGTTATAAACAAAAAAA

>TRINITY_DN121050_c4_g4_i2 from sample A1 / Example unspliced isoform:

CTTGAAGTTGAAGTTAATGTTACTGTTTGGTATTTCTTGTTGAAGTTACTGCGTTTTCTTTTGTTTATTCAGAGAAGAGTGATTGTTATAAGTAAAGATGGCACATAGGCCAGTTACGAGAGAGCTGGATGCCAAGGCGCGGGAGGGAGAGACTATCGTGCCCGGTGGCACCGGTGGGAAGTCCGTTGATGCCCAAGAGC
GTCTTGCTGAAGgtacttattgcgttgtattataaatatatatttcactgtattctagtaatataatatagttcttgacggttttcattgaattaaattgaagGGCGGAGCAGAGGTGGCCAGACTCGAAAGGAACAAATTGGCAGCGAAGGATACCAGGAGATGGGCCGCAAGGGAGGGCTTACCACACAAGACAAGTC
CGGTGCAGAACGGGCAGCTGAAGAGGGTATCCCCATTGATGAGTCCAAATACATGACCAATTGATTCCTGTTACCGTTTTCCTGCAATATATATACACATGGATATATATTGTATATATCCTCTGTTGTCATATAGTTATCGTTTCTTTGTGTTACATGTTAGTTTGATTTCAATAACCATTCGTTGGTTTCCTCTCCTT
TTTTAATGAAATAAATCTTCTTTGTTATAAACAAAAAAA

Intron 2 GTACTGATTG ... TGGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	2.63	370	13	7
A2	1.59	465	6	9
A3	3.56	542	21	19
A4	3.66	355	15	12
A5	4.23	340	17	13
A6	2.51	6785	205	145
A7	2.42	4801	134	104
A8	2.73	3658	112	93
A9	2.47	3336	107	62
A10	1.86	4021	81	71

>TRINITY_DN105360_c0_g1_i1 from sample A3 / Example spliced isoform

AGGCGATACTGCGTTTTGTTTTGTGTTTTCATTCAGAGAGAGTTTGTGAGAGAACAGTTAGCGTTAGAAGGAAAAATGGCACATAGGCAAGATGCGAGAGAGCTGGATGCCAAGGCGCGGGAGGGAGAGACTGTCGTGCCCGGTGGCACCGGTGGCAAGTCTCTTGAAGCCCAAGAGCATCTTGCTGAAGGGCGGAGCAG
AGGCGGCCAAACTCGAAAGGAACAAATAGGCAGCGAAGGGTACCAGGAGATGGGCCGCAAAGGAGGGCTCAGCACTCAAGACAAGTCCGGTGGAGAACGAGCAGCTGAAGAGGGTATTCCCATCGACGAGTCCAAATACAGGAAGAACTGATTCCAATTACCGTTTTCCCAATATATATACATAGATATATGTGGAATCA
CCGTTTTCCCAATATATATACATAGATATATGTATTGTATATATCCTCCGTGTTTCAACTTCCGGCATCATATTTCTTTTTCTTTCCAAATGTCTCTTATAATATAGTTATCGTTTCCTCTGTGGTGTACGTTGTTGTATGTCAAAAACCATTCGTTGGTTTGTTCTCCTGTGAATTTAATGAAATAAACCTTCTTTGTA
TATTTCATGAAATACAACTTCTTTGTTATATATACAGTGTACACTGTACTGCACTGGACTGAAATGAACCTTGTATTTAATGAAATAAGTTCCTTCTTTGTTATATACACTGGACTCGACTCTGCCTCGTAAAGTTTTGATGGAGTTCTCTATATTTCTATCGGAGTCTTTAAGATAAATACTTTTTAT

>TRINITY_DN105360_c0_g1_i3 from sample A3 / Example unspliced isoform:

AGGCGATACTGCGTTTTGTTTTGTGTTTTCATTCAGAGAGAGTTTGTGAGAGAACAGTTAGCGTTAGAAGGAAAAATGGCACATAGGCAAGATGCGAGAGAGCTGGATGCCAAGGCGCGGGAGGGAGAGACTGTCGTGCCCGGTGGCACCGGTGGCAAGTCTCTTGAAGCCCAAGAGCATCTTGCTGAAGgtactgattg
ccttgtattataaatatatatatttcaatattatagttctgacgattttcattgaattggtttgcagGGCGGAGCAGAGGCGGCCAAACTCGAAAGGAACAAATAGGCAGCGAAGGGTACCAGGAGATGGGCCGCAAAGGAGGGCTCAGCACTCAAGACAAGTCCGGTGGAGAACGAGCAGCTGAAGAGGGTATTCCCAT
CGACGAGTCCAAATACAGGAAGAACTGATTCCAATTACCGTTTTCCCAATATATATACATAGATATATGTGGAATCACCGTTTTCCCAATATATATACATAGATATATGTATTGTATATATCCTCCGTGTTTCAACTTCCGGCATCATATTTCTTTTTCTTTCCAAATGTCTCTTATAATATAGTTATCGTTTCCTCTGT
GGTGTACGTTGTTGTATGTCAAAAACCATTCGTTGGTTTGTTCTCCTGTGAATTTAATGAAATAAACCTTCTTTGTATATTTCATGAAATACAACTTCTTTGTTATATATACAGTGTACACTGTACTGCACTGGACTGAAATGAACCTTGTATTTAATGAAATAAGTTCCTTCTTTGTTATATACACTGGACTCGACTCT
GCCTCGTAAAGTTTTGATGGAGTTCTCTATATTTCTATCGGAGTCTTTAAGATAAATACTTTTTAT