sp|Q67ZB3|FRL3_ARATH ----> FRIGIDA-like protein 3

Intron 1 GTTTTTGCTG ... TGTGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.42	205.8	36.5	66.5
A2	32.05	120.3	16	56.5
A3	27.85	219	31.5	84.5
A4	25.93	146.8	19	51.3
A5	35.73	78.5	14	43.5
A6	25.84	102.3	15.5	35.8
A7	27.2	298.3	53	111.3
A8	28.53	163.5	17.5	65.5
A9	28.47	242.8	30.5	96.8
A10	29.31	289.8	46.5	120

>TRINITY_DN129634_c0_g1_i1 from sample A1 / Example spliced isoform

CTCCTGATGCCGACAGACGGGTTTACATTTTTCTCTTGGAATCCCTTTTCCCCATTTTTGGTAACGACGCCAGCGCTGATGTTCCATTGGATGTCAAGGATTCGGCCCGGGAGATTGCTAAGGTTTGGAGAAACCTGATTAAAAAGGAACGTGGGTTGGATAACCAGACCTTCTTGCAGCTCTTAATATCGTTTAGGATC
GCGACAGACTACGAGAAGGATGAGCTTTTGGAGATGTTATTTCCTTCTCCGGCATACAGCAAAGCTCCAGAGATGTGTCGGTCTCTTGGATTGTCTCCCCAAGATTTTATCCATAAGGTTTCCAACACTTATGGACCATTTCGAGCCATTAAAGCCGCTCTTGCATCTGGTATGATGGATAAAATAGATCCCGTGGTTTG
GTTGACATCATTTTTGATAGATGTGGAGAAGAATCTTACGGAGATGAGAGCACTCGAGCCGGATGGTTCTCAGCTACGCTGGACTAAAGCAGATAAGAAGAAGTTGGGCCGTTTGAAACGTGTTTTTAATCTGATCGAAATGGATGGCTTGCAATGTGATTCTTTCAAAGCCGTCATGCTGAAGGAAATAAAAAATTTGG
AACTGGAGAAGCTGGACAGGATAAAGAAAGCTGGTGAGAAAAAGCAACAGGAAGACAGAAAGAAAGTTGCTGAGAAAAAGGCACGGGAGAAAGAAGACAAAAAGGAAGCTTCTGCGAAAAAGGTACAGAGAGGCGATGGGCTTTCAGAGAGCGAAAACTATGCTTTAAAGAGGGCTAAGCATGCTTCACTGAACGAGACA
ACCAGAGTGGCTGGTGGTACTGACATTATTGGTCATAATCACCATCCATCAGCTTTTAGTGCTGCTAATAGGAGCTCCACTGGACTTGCTGCTGGTGCCTTCACATCAGCACTTTCCATGGCAGCTCAATTAAATGATGCGCGAAGACGTCACGAGGGGTACCTCTCAGTTTTGGGGACTAGCAATGTAACCCCTATTGG
ATCATCATTATACAGCTCTGGAGTCCCTGCTGGTGCTTTTTCAGTCCCATTGTCCAATGTGGCTGAAGTTAATTATGAGGGAAGAGCTGAGCAAAGCTATATGTCGAATTTTGGTGAAGTTGATAAAAATCATGTTAGAGCATCAGTATATGGGAC

>TRINITY_DN129634_c0_g1_i10 from sample A1 / Example unspliced isoform:

CTCCTGATGCCGACAGACGGGTTTACATTTTTCTCTTGGAATCCCTTTTCCCCATTTTTGGTAACGACGCCAGCGCTGATGTTCCATTGGATGTCAAGGATTCGGCCCGGGAGATTGCTAAGGTTTGGAGAAACCTGATTAAAAAGGAACGTGGGTTGGATAACCAGACCTTCTTGCAGCTCTTAATATCGTTTAGGATC
GCGACAGACTACGAGAAGGATGAGCTTTTGGAGATGTTATTTCCTTCTCCGGCATACAGCAAAGCTCCAGAGATGTGTCGGTCTCTTGGATTGTCTCCCCAAGATTTTATCCATAAGGTTTCCAACACTTATGGACCATTTCGAGCCATTAAAGCCGCTCTTGCATCTGGTATGATGGATAAAATAGATCCCGTGGTTTG
GTTGACATCATTTTTGATAGATGTGGAGAAGAATCTTACGGAGATGAGAGCACTCGAGCCGGATGGTTCTCAGgtttttgctgatgtttatatccttcttttaagatttaaagggacttgatgtaacactgaattgaaaatttataatttgatttgtgctgcagCTACGCTGGACTAAAGCAGATAAGAAGAAGTTGGGC
CGTTTGAAACGTGTTTTTAATCTGATCGAAATGGATGGCTTGCAATGTGATTCTTTCAAAGCCGTCATGCTGAAGGAAATAAAAAATTTGGAACTGGAGAAGCTGGACAGGATAAAGAAAGCTGGTGAGAAAAAGCAACAGGAAGACAGAAAGAAAGTTGCTGAGAAAAAGGCACGGGAGAAAGAAGACAAAAAGGAAGC
TTCTGCGAAAAAGGTACAGAGAGGCGATGGGCTTTCAGAGAGCGAAAACTATGCTTTAAAGAGGGCTAAGCATGCTTCACTGAACGAGACAACCAGAGTGGCTGGTGGTACTGACATTATTGGTCATAATCACCATCCATCAGCTTTTAGTGCTGCTAATAGGAGCTCCACTGGACTTGCTGCTGGTGCCTTCACATCAG
CACTTTCCATGGCAGCTCAATTAAATGATGCGCGAAGACGTCACGAGGGGTACCTCTCAGTTTTGGGGACTAGCAATGTAACCCCTATTGGATCATCATTATACAGCTCTGGAGTCCCTGCTGGTGCTTTTTCAGTCCCATTGTCCAATGTGGCTGAAGTTAATTATGAGGGAAGAGCTGAGCAAAGCTATATGTCGAAT
TTTGGTGAAGTTGATAAAAATCATGTTAGAGCATCAGTATATGGGAC

Intron 2 GTTTTGCAGA ... TGTGCTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.23	87	4.3	18.7
A2	16.42	38.3	4	7.7
A3	15.4	74.3	1	14.7
A4	24.68	41.3	8	13.7
A5	33.79	17.3	2.7	8.3
A6	9.52	3	1.3	0.7
A7	22.36	55.3	5	16.3
A8	14.79	44	0.3	9
A9	22.08	52.3	3	16.3
A10	22.9	56.3	5.7	17

>TRINITY_DN48769_c0_g1_i2 from sample A7 / Example spliced isoform

ACCCTAAATATAAAGGAGGAAAACCCAGAAGAATCCGCAACCCTGGGCAACAACAAAGATAACAAGACATCAAATGTCCAAGTCATCGATGGGCTCAAAACAATCTGCGATAATGTCGACGGTGGTGGTCTCGTAAGATACATCGTGCGGAACATGAGAGACGAGAAAACCCTTCGGAAGGATTTGTCGGTGGCGCTCAG
TAACGCCCGCGATCCGGCGAAGCTGGCGTTGAAAGCTCTGCAGGGCTATAATTTCTCAAAATTTGGCATCAGATGGGCTTGCCTTTTGATCTTGGAATACCTTTTCCCCATTTTTGGTAACGACGACAGCGATGCTGTTCCATTGCATGTCAAGAATTCGGCCCGGGAGATTGCTAAGGTTTGGAGAAACCTGATTAAAA
AGGAACGTGGGTTGGATAACCAGACCTTCTTGCAGCTCTTAATATCGTTTAGGATTGCGACAGACTACGAGAAGGATGAGCTTTTGGAGATGTTATTTCCTTCGGATGAATAGGAAATCTGCATTGGATAGCAATGCCTTCCTGCAGCTCTTAATATCGTTCAGGATCGGGGCGGATTACGAGAAGGACGAGCTTTTGGA
GATACTGATTCCTGCTTTGAGCCACAAGCAAGCTCCAGAGCTGTGTCGGTCTCTTGGATTGTTTCCCGATGCAGATTTTCTGGATAAGGTTTCCAAAAATCTTGGACCGTTAGATGTCCTTAGATTTGCTCATGCATTTGGCATGATGGAAAAAGTAGATCCTGTGGGTTTGTTGACATCATTTTTGAGAGATGAGGAGA
AGGATATTACAGAGATGAGAGCACACAATCCAGATGCTTCTCAGATACTCTGGACTGATGCAGATATTAAGAAGATGGGCGCTTTGAAAAGTGTTATTAATCATGTCGAAATGTATGGTTTTTGAGAGATGAGGAGAAGGATATTACAGAGATGAGAGCACACAATCCAGATGCTTCTCAGATACTCTGGACTGATGCAG
ATATTAAGAAGATGGGCGCTTTGAAAAGTGTTATTAATCATGTTCTGGACTGATGCAGATATTAAGAAGATGGGCGCTTTGAAAAATGTTATTTATCATGTCGAAATCTCGAAATGTATGGTTTGCAATGTGATTCTTTCAAAGACAACCTGCAGAACGAACTAAGAGAATTGGAAGTTGAGAGAGAGCGCAGAAGAAAA
TTGAAGCCTAAGAGAAAGCGCAGAAAAGTAGCTCCTGAGAAAAAGGCACAGGAGAAAGAAGACAAAAAGGAAGCTTCTGCGAAAAAGGTACAGAGAGGCGATGGGCTTTCAGAGAGCGAAAACTATGCTTTAAAGAGGGCTAAGCATGCTTCACTGAACGAGACAACCAGAGTGGCTGGTGGTACTGACATTATTGGTCA
TAATCACCATCCATCAGCTTTTAGTGCTGCTAATAGGAGCTCCACTGGA

>TRINITY_DN48769_c0_g1_i6 from sample A7 / Example unspliced isoform:

ACCCTAAATATAAAGGAGGAAAACCCAGAAGAATCCGCAACCCTGGGCAACAACAAAGATAACAAGACATCAAATGTCCAAGTCATCGATGGGCTCAAAACAATCTGCGATAATGTCGACGGTGGTGGTCTCGTAAGATACATCGTGCGGAACATGAGAGACGAGAAAACCCTTCGGAAGGATTTGTCGGTGGCGCTCAG
TAACGCCCGCGATCCGGCGAAGCTGGCGTTGAAAGCTCTGCAGGGCTATAATTTCTCAAAATTTGGCATCAGATGGGCTTGCCTTTTGATCTTGGAATACCTTTTCCCCATTTTTGGTAACGACGACAGCGATGCTGTTCCATTGCATGTCAAGAATTCGGCCCGGGAGATTGCTAAGGTTTGGAGAAACCTGATTAAAA
AGGAACGTGGGTTGGATAACCAGACCTTCTTGCAGCTCTTAATATCGTTTAGGATTGCGACAGACTACGAGAAGGATGAGCTTTTGGAGATGTTATTTCCTTCGGATGAATAGGAAATCTGCATTGGATAGCAATGCCTTCCTGCAGCTCTTAATATCGTTCAGGATCGGGGCGGATTACGAGAAGGACGAGCTTTTGGA
GATACTGATTCCTGCTTTGAGCCACAAGCAAGCTCCAGAGCTGTGTCGGTCTCTTGGATTGTTTCCCGATGCAGATTTTCTGGATAAGGTTTCCAAAAATCTTGGACCGTTAGATGTCCTTAGATTTGCTCATGCATTTGGCATGATGGAAAAAGTAGATCCTGTGGGTTTGTTGACATCATTTTTGAGAGATGAGGAGA
AGGATATTACAGAGATGAGAGCACACAATCCAGATGCTTCTCAGATACTCTGGACTGATGCAGATATTAAGAAGATGGGCGCTTTGAAAAGTGTTATTAATCATGTCGAAATGTATGGTTTTTGAGAGATGAGGAGAAGGATATTACAGAGATGAGAGCACACAATCCAGATGCTTCTCAGgttttgcagatgtttatat
ccttcttttaagattaaaagggacttgatgtaacactgaattgaaaatttatattttgatgtgtgctgtagATACTCTGGACTGATGCAGATATTAAGAAGATGGGCGCTTTGAAAAGTGTTATTAATCATGTTCTGGACTGATGCAGATATTAAGAAGATGGGCGCTTTGAAAAATGTTATTTATCATGTCGAAATCTC
GAAATGTATGGTTTGCAATGTGATTCTTTCAAAGACAACCTGCAGAACGAACTAAGAGAATTGGAAGTTGAGAGAGAGCGCAGAAGAAAATTGAAGCCTAAGAGAAAGCGCAGAAAAGTAGCTCCTGAGAAAAAGGCACAGGAGAAAGAAGACAAAAAGGAAGCTTCTGCGAAAAAGGTACAGAGAGGCGATGGGCTTTC
AGAGAGCGAAAACTATGCTTTAAAGAGGGCTAAGCATGCTTCACTGAACGAGACAACCAGAGTGGCTGGTGGTACTGACATTATTGGTCATAATCACCATCCATCAGCTTTTAGTGCTGCTAATAGGAGCTCCACTGGA