NA ----> NA

Intron 1 GTTTGTGACC ... ATGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84.61	7	12	49
A2	72.5	6.5	5	27.5
A3	66.79	12	9	39
A4	55.5	16.5	8	32.5
A5	72.81	5.5	9	20
A6	50.11	54	26	81.5
A7	62.02	88	73.5	208.5
A8	54.06	88	60	146.5
A9	38.28	106	34.5	97
A10	51.77	103	88	132

>TRINITY_DN47324_c0_g1_i6 from sample A7 / Example spliced isoform

GTACTTTATAAATTTGTTGATCTGTCATTGTACCGTTAGAAAAGGTTTTCCATGGATCTTTTTATTGAAAAGATTTTGGCAACTGGTACTTTATGGTATTTAAAACCACCTTGTATTCTGGCCTTCGATGCTCCCTTAACTTGTATTTTTGAAGGATATCCGGCCAATATTTTGATGAACTTGCAAGAAGATGGTGGTGG
ATCTGATCTTAAGAAAATAAATTGCAAGGAGATGGCGGTGTATCAGAAATTCCGAAAAGAAAAAGCATACATTGGAATTAGAATAGCTTGGTCAACTGATTTCATTTTGTAGCAGAGGAGTTGAAACCATTGATCTCAGAGGAGAGAATACTTGAATATGGTTTTGCAATGAGCTGCCTCGGGCCATTGCATTTTTTAAA

>TRINITY_DN47324_c0_g1_i9 from sample A7 / Example unspliced isoform:

GTACTTTATAAATTTGTTGATCTGTCATTGTACCGTTAGAAAAGGTTTTCCATGGATCTTTTTATTGAAAAGATTTTGGCAACTGGTACTTTATGGTATTTAAAACCACCTTGTATTCTGGCCTTCGATGCTCCCTTAACTTGTATTTTTGAAGGATATCCGGCCAATATTTTGATGAACTTGCAAGAAGATGGTGGTGG
ATCTGATCTTAAGAAAATAAATTGCAAGGAGATGGCGGTGTATCAGAAATTCCGAAAAGAAAAAGCATACATTGGAATTAGAATgtttgtgaccgtttgaattgtatcggatataaaagatttgaatatttgagacaaccatcgttgtgctagtttcatctcagcttctgtattctgcatttgtttcatggtttttctta
tttttctttttggtttgttttgtttcagcgccaatcatgtttgttggtttttacagtccattttctctacctacgttttggctgtagctgtctttcctggttgatcttttttcatttttttggtttatgtttgcagAGCTTGGTCAACTGATTTCATTTTGTAGCAGAGGAGTTGAAACCATTGATCTCAGAGGAGAGAA
TACTTGAATATGGTTTTGCAATGAGCTGCCTCGGGCCATTGCATTTTTTAAA

Intron 2 GTATGTGCCT ... ATGATATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.61	17	9	6
A2	38.24	21	20	6
A3	39.53	13	8	9
A4	60	4	8	4
A5	40	3	3	1
A6	54.88	37	54	36
A7	35.53	127	98	42
A8	36.36	112	75	53
A9	47.97	64	71	47
A10	40.76	72.7	62	38

>TRINITY_DN120753_c1_g3_i6 from sample A3 / Example spliced isoform

GCTTTCTTGGTTTTTGTTCTTTTTCTTTTTGGTTTGTTTTGTTTCAGGGCCAAGCATGTTTGTTGGTTTTTACAGTCCATTTTCTCTACCTACGTTTTGGCTGTAGCTGGCTTTCCTGGTTGATCTTTTTTCATTTTTTTGGTTTATGTTTGCAGAGCTTGGTCAACTGATTTCATTTTGTAGCAGAGGAGTTGAAACCA
TTGATCTCAGAGGAGAGAATTCTTTAATATGGTTTTGCAATGAGCTGCTCGGTCCATTGCATCTTTTAATTTGTTGCCCACAATGATCAGATATCGACCCATTACAATTTCAACGGCCTTCACATATTTCATCTTGGATGTCTGCGATTCGAGAATCTTGGGGAGGCAGTCGGGAGCTGTGACATTGAAGGACGACGAAA
TTTTTACATCGGAGGTGTTTAGCTATGATATTGAAGAGTGGAAACTACTGCTAATGCTAAGGGAATTGGAGTCACTTTGTTGATACATAGTGCCTGATCGACGATATCCCCTGCCACGACATTTACGGTCAGTAATCGC

>TRINITY_DN120753_c1_g3_i1 from sample A3 / Example unspliced isoform:

CCAATATTTTGATGAACTTGCAAGAAGATGGTGGTGGATCTGATCTTAAGAAAATAAATTGCAAGGAGATGGCGGTGTATCAGAAATTCCGAAAAGAAAAAGCATACATTGGAATTAGAATAGCTTGGTCAACTGATTTCATTTTGTAGCAGAGGAGTTGAAACCATTGATCTCAGAGGAGAGAATTCTTTAATATGGTT
TTGCAATGAGCTGCTCGGTCCATTGCATCTTTTAATTTGTTGCCCACAATGATCAGATATCGACCCATTACAATTTCAACGGCCTTCACATATTTCATCTTGGgtatgtgccttacacatgtttcatattggtatgttccattggagcaaaggaatttctcccacagaaataattctttgatacgtccaactcatgatat
tagATGTCTGCGATTCGAGAATCTTGGGGAGGCAGTCGGGAGCTGTGACATTGAAGGACGACGAAATTTTTACATCGGAGGTGTTTAGCTATGATATTGAAGAGTGGAAACTACTGCTAATGCTAAGGGAATTGGAGTCACTTTGTTGATACATAGTGCCTGATCGACGATATCCCCTGCCACGACATTTACGGTCAGTA
ATCGC

Intron 3 GTTTGTGACC ... GTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	83.12	13	12	64
A2	79.07	9	6	34
A3	78.13	14	7	50
A4	66.1	20	7	39
A5	75.86	7	10	22
A6	60	62	21	93
A7	69.49	108	63	246
A8	63.1	100	51	171
A9	48.44	116	45	109
A10	57.66	116	86	158

>TRINITY_DN45489_c0_g3_i3 from sample A8 / Example spliced isoform

AATTTGTTGATCTGTCATTGTACGGTTAGAGAAGGTTTTCCATGGATATTTTTATTGAAAATATTTTGGCAACTGGTACTTTATGGTATTTAAAACCACCTTGTATTCTGGCCTTCGATGCTCCCTTAACTTGTATTTTTGAAGGATATCCGGCCAATATTTTGATGAACTTGCAAGAAGATGGTGGTGGATCTGATCTT
AAGAAAATAAATTGCAAGGAGATGGCGGTGTATCGGAAATTCCGAAAAGAAAAAGCATACATTGGAATTAGAATAGCTTGGTCAACTGATTTCATTTTGTAGCAGAGGAGTTGAAACCATTGATCTCAGAGGAGAGAATTCTTTAATATGGTTTTGCAATGAGCTGCTCGGTCCATTGCATCTTTTAATTTGTTGCC

>TRINITY_DN45489_c0_g3_i2 from sample A8 / Example unspliced isoform:

AATTTGTTGATCTGTCATTGTACGGTTAGAGAAGGTTTTCCATGGATATTTTTATTGAAAATATTTTGGCAACTGGTACTTTATGGTATTTAAAACCACCTTGTATTCTGGCCTTCGATGCTCCCTTAACTTGTATTTTTGAAGGATATCCGGCCAATATTTTGATGAACTTGCAAGAAGATGGTGGTGGATCTGATCTT
AAGAAAATAAATTGCAAGGAGATGGCGGTGTATCGGAAATTCCGAAAAGAAAAAGCATACATTGGAATTAGAATgtttgtgaccgttttaaatggacttttgaccgtttgaattgtatcggatataaaagatttgaatatttgagacaaccatcgttgtgctagtttcatctcagcttctgtattctgcatttgtttcat
ggtttttcttatttttctttttggtttgttttgtttcagcgccaatcatgtttgttggtttttacagtccattttctctacctacgttttggctgtagctggctttcctggttgatcttttttcatttttttggtttgtgtttgcagAGCTTGGTCAACTGATTTCATTTTGTAGCAGAGGAGTTGAAACCATTGATCTC
AGAGGAGAGAATTCTTTAATATGGTTTTGCAATGAGCTGCTCGGTCCATTGCATCTTTTAATTTGTTGCC