NA ----> NA

Intron 1 GTTCAGCTTT ... ATGAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.96	122.1	62	47.3
A2	25.77	144.7	50.8	49.5
A3	27.11	138.5	60	43
A4	29.58	68.9	31	26.8
A5	35.85	61.1	46	22.3
A6	27.75	286.7	132.5	87.7
A7	28.04	610.8	286.2	189.8
A8	32.22	476.3	266.5	186
A9	32.28	634.2	345.3	258.7
A10	28.79	626.5	281.3	224.8

>TRINITY_DN118796_c0_g1_i10 from sample A1 / Example spliced isoform

TTGTTATGTTAAAAAAGAAGAAAGAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACA
TGGCGGAACCTCCGGTTAGACTTATGGATTTCGTCTCTGAACAGCAGTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCGATTTAAGCATAGGCCTCCAAAAGCACTCGAT
GACGATGAGACACAGTTTTTGGAGAACATGGAGATGTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACCTGAAACAGGCAGACAAAAGGACAACTCAAGC
ACGGCGTCCCTTGAGCAGCTTAATTATGATCAAGCCACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAAATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCTGATACTGGACTTATGGGATTGGTATCTTACA
GTGATGAAAGTGATGAGGATGCTGAATAATTATTTAGAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATTATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTGGGTTGATCTGTGATTTATTCTGCTTACCTGA
CTATGTTATTTCATTGATATAGTCAAATACATGAAAATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACATCAAAGAAACCAACGGCTAATGTGGGCTTTCAGGTATGTTTTTTTCATTTGATGTGCCAAAATTCAACAATTGCATGCATTA
TTTACCTTGAATTAAGGAAAGGAGTAGATGCTGATGAATAATGCAATTTCAATTGTTGTACAGTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGCGTTTATTAAATCATATCTTGTGTGTTACTTATTGAGGC

>TRINITY_DN118796_c0_g1_i16 from sample A1 / Example unspliced isoform:

TTGTTATGTTAAAAAAGAAGAAAGAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACA
TGGCGGAACCTCCGGTTAGACTTATGGATTTCGTCTCTGAACAGCAGgttcagctttaaaataaagaaatgaatgcagTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCG
ATTTAAGCATAGGCCTCCAAAAGCACTCGATGACGATGAGACACAGTTTTTGGAGAACATGGAGATGTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACC
TGAAACAGGCAGACAAAAGGACAACTCAAGCACGGCGTCCCTTGAGCAGCTTAATTATGATCAAGCCACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAAATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCT
GATACTGGACTTATGGGATTGGTATCTTACAGTGATGAAAGTGATGAGGATGCTGAATAATTATTTAGAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATTATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTG
GGTTGATCTGTGATTTATTCTGCTTACCTGACTATGTTATTTCATTGATATAGTCAAATACATGAAAATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACATCAAAGAAACCAACGGCTAATGTGGGCTTTCAGGTATGTTTTTTTCATTTG
ATGTGCCAAAATTCAACAATTGCATGCATTATTTACCTTGAATTAAGGAAAGGAGTAGATGCTGATGAATAATGCAATTTCAATTGTTGTACAGTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGCGTTTATTAAATCATATCTTGTGTGTTACTTATTGAGGC

Intron 2 GTATGTTTTT ... TGTTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	2	1	3
A2	38.46	4	2	3
A3	71.43	1	2	3
A4	100	0	1	0
A5	60	1	2	1
A6	66.67	3	8	4
A7	48.48	17	21	11
A8	56.39	29	49	26
A9	75.34	9	36	19
A10	45	11	14	4

>TRINITY_DN45086_c0_g1_i7 from sample A7 / Example spliced isoform

TCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACCTGAAACAGGCAGACAAAAGGACAACTCAAGCACGGCGTCCCTTGAGCAGCTTAATTATGATCAAGCC
ACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAAATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCTGATACTGGACTTATGGGATTGGTATCTTACAGTGATGAAAGTGATGAGGATGCTGAATAATTATTTA
GAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATTATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTGGGTTGATCTGTGATTTATTCTGCTTACCTGACTATGTTATTTCATTGATATAGTCAAATACATGAAA
ATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACATCAAAGAAACCAACGGCTAATGTGGGCTTTCAGTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGCGTTTATTAAATCATATCTTGTGTGTTACTTATTG
AGGCAATGCTAATCATATATTGCAATTAATATTTCATCTCAAGGAGAATAACAAGTTTTCACACATTTGATTTTAATATATACAGAGTAGTTTGATTGTGGAAAAAGTGTTTACAGGGGAGTTCTTATTGAAATTTCAGATTCCCACACCCCACGAAAGAAAATGAAAAGAAACACTCTTTGTTTGGTGGCTTGAAGGAC
AAATTCTTTAGTTTGAACTTTAACAGTAGGTTAAGGGAGAGAAATTGTTGCTGCC

>TRINITY_DN45086_c0_g1_i6 from sample A7 / Example unspliced isoform:

GAAAGAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACATGGCGGAACCTCCGGTTAG
ACTTATGGATTTCGTCTCTGAACAGCAGTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCGATTTAAGCATAGGCCTCCAAAAGCACTCGATGACGATGAGACACAGTTTT
TGGAGAACATGGAGATGGTGAGCACGGATTTTAGAGCTGTTCATTTATTGGTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACCTGAAACAGGCAGACAA
AAGGACAACTCAAGCACGGCGTCCCTTGAGCAGCTTAATTATGATCAAGCCACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAAATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCTGATACTGGACTTATGG
GATTGGTATCTTACAGTGATGAAAGTGATGAGGATGCTGAATAATTATTTAGAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATTATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTGGGTTGATCTGTGATTT
ATTCTGCTTACCTGACTATGTTATTTCATTGATATAGTCAAATACATGAAAATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACATCAAAGAAACCAACGGCTAATGTGGGCTTTCAGgtatgtttttttcatttgatgtgccaaaattcaa
caattgcatgcattatttaccttgaattaaggaaaggagtagatgctgatgaataatgcaatttcaattgttgtacagTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGCGTTTATTAAATCATATCTTGTGTGTTACTTATTGAGGCAATGCTAATCATATATTGCAATTAATATTTCAT
CTCAAGGAGAATAACAAGTTTTCACACATTTGATTTTAATATATACAGAGTAGTTTGATTGTGGAAAAAGTGTTTACAGGGGAGTTCTTATTGAAATTTCAGATTCCCACACCCCACGAAAGAAAATGAAAAGAAACACTCTTTGTTTGGTGGCTTGAAGGACAAATTCTTTAGTTTGAACTTTAACAGTAGGTTAAGGG
AGAGAAATTGTTGCTGCC

Intron 3 GTGAGCACGG ... CATTTATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.52	33	39	37
A2	32.58	30	14	15
A3	40.94	44	34	27
A4	29.58	25	14	7
A5	40.39	15	10.3	10
A6	43.62	67	54	49.7
A7	39.46	224	179	113
A8	40.98	168	139.3	94
A9	41.44	187	151	113.7
A10	35.43	164	95	85

>TRINITY_DN118796_c0_g1_i10 from sample A1 / Example spliced isoform

TTGTTATGTTAAAAAAGAAGAAAGAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACA
TGGCGGAACCTCCGGTTAGACTTATGGATTTCGTCTCTGAACAGCAGTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCGATTTAAGCATAGGCCTCCAAAAGCACTCGAT
GACGATGAGACACAGTTTTTGGAGAACATGGAGATGTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACCTGAAACAGGCAGACAAAAGGACAACTCAAGC
ACGGCGTCCCTTGAGCAGCTTAATTATGATCAAGCCACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAAATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCTGATACTGGACTTATGGGATTGGTATCTTACA
GTGATGAAAGTGATGAGGATGCTGAATAATTATTTAGAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATTATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTGGGTTGATCTGTGATTTATTCTGCTTACCTGA
CTATGTTATTTCATTGATATAGTCAAATACATGAAAATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACATCAAAGAAACCAACGGCTAATGTGGGCTTTCAGGTATGTTTTTTTCATTTGATGTGCCAAAATTCAACAATTGCATGCATTA
TTTACCTTGAATTAAGGAAAGGAGTAGATGCTGATGAATAATGCAATTTCAATTGTTGTACAGTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGCGTTTATTAAATCATATCTTGTGTGTTACTTATTGAGGC

>TRINITY_DN118796_c0_g1_i5 from sample A1 / Example unspliced isoform:

TTGTTATGTTAAAAAAGAAGAAAGAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACA
TGGCGGAACCTCCGGTTAGACTTATGGATTTCGTCTCTGAACAGCAGTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCGATTTAAGCATAGGCCTCCAAAAGCACTCGAT
GACGATGAGACACAGTTTTTGGAGAACATGGAGATGgtgagcacggattttagagctgttcatttattagTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGG

Intron 4 GTGAGCACGG ... TTATTCCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.92	33	26	11
A2	30.23	30	14	12
A3	26.67	44	22	10
A4	26.47	25	14	4
A5	26.83	15	7	4
A6	30.93	67	32	28
A7	29.13	225	142	43
A8	28.36	168	98	35
A9	31	187	116	52
A10	26.79	164	71	49

>TRINITY_DN37570_c0_g1_i5 from sample A6 / Example spliced isoform

GAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACATGGCGGAACCTCCGGTTAGACTT
ATGGATTTCGTCTCTGAACAGCAGTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCGATTTAAGCATAGGCCTCCAAAAGCACTCGATGACGATGAGACACAGTTTTTGGA
GAACATGGAGATGTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATACAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACCTGAAACAGGCAGACAAAAGGACAACTCAAGCACGGCGTCCCTTGAGCAGCTTAA
TTATGATCAAGCCACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAAATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCTGATACTGGACTTATGGGATTGGTATCTTACAGTGATGAAAGTGATGAGGATGCT
GAATAATTATTTAGAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATTATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTGGGTTGATCTGTGATTTATTCTGCTTACCTGACTATGTTATTTCATTGATATAGT
CAAATACATGAAAATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACATCAAAGAAACCAACGGCTAATGTGGGCTTTCAGGTATGTTTTTTTCATTTGATGTGCCAAAATTCAACAATTGCATGCATTATTTACCTTGAATTAAGGAAAGGA
GTAGATGCTGATGAATAATGCAATTTCAATTGTTGTACAGTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGC

>TRINITY_DN37570_c0_g1_i2 from sample A6 / Example unspliced isoform:

GAAGAAGTGAAACAAAGAATGGACTTCACCTTGCAAAACGTAAACGCTGTGTGCTATAAGGCCTTAACGGGGGAGAAATTCGATTCGGTGCTCTCAAATTAAGTGGAACTGGGTAGGGAGAAATTTGTTATTCGCAAAATCTAGTTAGCATGGAAGACCGAACAAAGAGTGAAAACATGGCGGAACCTCCGGTTAGACTT
ATGGATTTCGTCTCTGAACAGCAGGTTCAGCTTTAAAATAAAGAAATGAATGCAGTTGGAGGAGTTCAGGGAGAAGCGTGGAGCGCGGGTAGAGGACGGGACGGTTCAGAGAGACAGACCCCTTTACGAGATTCTAAAGGAGAATAAAGACAAACAGCAAGCAGAATTCAATGAGCGATTTAAGCATAGGCCTCCAAAAG
CACTCGATGACGATGAGACACAGTTTTTGGAGAACATGGAGATGgtgagcacggattttagagctgttcatttattggtctctcagaagtggcgtggtgaatttatcattattcccagTCTAAGAGGGAGTTAGAACAACAACTGGCAAATGAGGAGGCTCAACAACTTCAAAAATTTAAAGCAGCTGTAGCAAGTCATA
CAATAAATACCATTGTTTCCAAGGATCCAGTTCCACGAAAATCAGAGGACCTGAAACAGGCAGACAAAAGGACAACTCAAGCACGGCGTCCCTTGAGCAGCTTAATTATGATCAAGCCACAAGCAAAAAAGTTAAAAATGGATTCAGAGAAGAGTAGTGAACGTGTTGAGTTATCTAAAATGCTGGATGGAGTTGAGAAA
ATACCAGCAAAGGATGGGCCAAAGAAAGAGGAGATAGTTACCAGTAGTGCTGATACTGGACTTATGGGATTGGTATCTTACAGTGATGAAAGTGATGAGGATGCTGAATAATTATTTAGAACACAAATATCTTTTTTGGTCCATGTTGTGTTTCTGTGTTATGTGTAAGCAATTCTTATCAGGCATGAGATGTTTTCATT
ATGCTCATGTTAGACATGTCTGCAAGTGAATTATCTCATCTGGGTATGTTGGGTTGATCTGTGATTTATTCTGCTTACCTGACTATGTTATTTCATTGATATAGTCAAATACATGAAAATCACAACCAGGTAATTCACTGTATTCTTTTTAGTTAAATGACATGAAATACAAGAAGGCCTTGAATTATTTTCCCTAAACA
TCAAAGAAACCAACGGCTAATGTGGGCTTTCAGGTATGTTTTTTTCATTTGATGTGCCAAAATTCAACAATTGCATGCATTATTTACCTTGAATTAAGGAAAGGAGTAGATGCTGATGAATAATGCAATTTCAATTGTTGTACAGTCTTTTCCATCGTTACTGAGATTCAAGCTTGTTTGCGAAGAAGAGTTTGGC