sp|Q682U6|DPD1_ARATH ----> Exonuclease DPD1, chloroplastic/mitochondrial

Intron 1 GTACATGTGG ... ATGTTCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.54	82	104	57
A2	55.35	73	115	66
A3	51.67	87	121	65
A4	48.94	96	129	55
A5	51.41	43	67	24
A6	50.51	145	137	159
A7	52.48	264	339	244
A8	55.31	141	134	215
A9	52.58	267	318	274
A10	51.24	245	279	236

>TRINITY_DN47205_c4_g1_i2 from sample A7 / Example spliced isoform

TTACATTTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAGTGGAAAGATCTTGCTCC
TATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAAAGTAATGAAGTTGAATGGGTCAGTGCGTG
GTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCGAGCAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATA
GAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTATACCTCTGCATGGGAGACAAAAATTGCATTACCATATGGATGCACTTGCTATGGTTTCATAGGATTATAAGATTAA
CAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTG

>TRINITY_DN47205_c4_g1_i6 from sample A7 / Example unspliced isoform:

TTACATTTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAgtacatgtggagtgtcca
gatatggagggcttgtttcaatatgcagaatccatatgatatggaggagtatccagcacaaaacctgctcagcttcacaaggtagcaagcacttttaagagcagcacctttgggttcccacaatccaagcagcatggcatcagcgaaaagagtgctccagcagctagaaatattgttaataaacattatatgcataccac
ggcagcaaaagtgggccgaaaaaaatgcagtactcatagatcaaaaataaaggaagctactgatggtccattgaagcattctgaaggaaaagatttgagtcagaagccaatagtcatggtgtttgatcttgaaactactggcttaagttgtatcaacgacagaattattgaatttgctgctcaagatcttgttggtggac
ggcatagtacatttcagtctttggtaaatcctggatgctttgtgacaaatgaaaagattcacggtatcagcaatcaaatggtcaataggtctaatgttccaagGTGGAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTT
GATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAAAGTAATGAAGTTGAATGGGTCAGTGCGTGGTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCGAGCAATGTCAGATGT
CAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTAC
ACAGGAGACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTATACCTCTGCATGGGAGACAAAAATTGCATTACCATATGGATGCACTTGCTATGGTTTCATAGGATTATAAGATTAACAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTG

Intron 2 GTCAGTTTTG ... GAGTTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.25	28	48	67
A2	69.66	27	58	66
A3	65.31	34	60	68
A4	62.38	38	65	61
A5	71.96	15	37	40
A6	66.67	32	71	57
A7	70.02	61	154	131
A8	66.28	58	103	125
A9	70.15	70	161	168
A10	63.64	82	140	147

>TRINITY_DN101866_c0_g1_i1 from sample A5 / Example spliced isoform

TTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAGTACATGTGGAGTGTCCAGATATG
GAGGGCTTGTTTCAATATGCAGAATCCATATGATATGGAGGAGTATCCAGCACAAAACCTGCTCAGCTTCACAAGGTAGCAAGCACTTTTAAGAGCAGCACCTTTGGGTTCCCACAATCCAAGCAGCATGGCATCAGCGAAAAGAGTGCTCCAGCAGCTAGAAATATTGTTAATAAACATTATATGCATACCACGGCAGC
GAAAGTGGGCCGAAAAAAATGCAGTACTCATAGATCAAAAATAAAGGAAGCTACTGATGGTCCATTGAAGCATTCTGAAGGAAAAGATTTGAGTCAGAAGCCAATAGTCATGGTGTTTGATCTTGAAACTACTGGCTTAAGTTGTATCAACGACAGAATTATTGAATTTGCTGCTCAAGATCTTGTTGGTGGACGGCATA
GTACATTTCAGTCTTTGGTAAATCCTGGATGCTTTGTGACAAATGAAAAGATTCACGGTATCAGCAATCAAATGGTCAATAGGTCTAATGTTCCAAGGTGGAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCA
CGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAAAGTAATGAAGTTGAATGGGTCAGTGCGTGGTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCAAGCAATGTCAGATGTCAATAC
ACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGA
GACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTATACCTCTGCATGGGAGACAAAAATTGCATTACCATATGGATGCACTTGCTATGGTTTCATAGGATTATAAGATTAACAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTG

>TRINITY_DN101866_c0_g1_i4 from sample A5 / Example unspliced isoform:

TTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAGTACATGTGGAGTGTCCAGATATG
GAGGGCTTGTTTCAATATGCAGAATCCATATGATATGGAGGAGTATCCAGCACAAAAgtcagttttgcagccttttggctttcacaaagttctctatgcaaaatattggttactccatgagatacattcctttcattcatagcttacaccttctgagtttccagCCTGCTCAGCTTCACAAGGTAGCAAGCACTTTTAAG
AGCAGCACCTTTGGGTTCCCACAATCCAAGCAGCATGGCATCAGCGAAAAGAGTGCTCCAGCAGCTAGAAATATTGTTAATAAACATTATATGCATACCACGGCAGCGAAAGTGGGCCGAAAAAAATGCAGTACTCATAGATCAAAAATAAAGGAAGCTACTGATGGTCCATTGAAGCATTCTGAAGGAAAAGATTTGAG
TCAGAAGCCAATAGTCATGGTGTTTGATCTTGAAACTACTGGCTTAAGTTGTATCAACGACAGAATTATTGAATTTGCTGCTCAAGATCTTGTTGGTGGACGGCATAGTACATTTCAGTCTTTGGTAAATCCTGGATGCTTTGTGACAAATGAAAAGATTCACGGTATCAGCAATCAAATGGTCAATAGGTCTAATGTTC
CAAGGTGGAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAAAGTAATG
AAGTTGAATGGTAGGTGGCTTTCAATTTCTGTTTAGATTACTGTTGTCGCATTTAAAAATAAAGATTGTCGATTGATGTTTTATGCCGTCTTGTCCCTTTGCTTCCGAAAGTGGCTTCT

Intron 3 GTACATGTGG ... ATGTTCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.85	82	106	57
A2	57.18	73	129	66
A3	53.48	87	135	65
A4	50.39	96	140	55
A5	53.01	43	73	24
A6	51.01	145	143	159
A7	53.23	264	357	244
A8	56.21	141	147	215
A9	53.57	267	342	274
A10	51.77	245	290	236

>TRINITY_DN106488_c0_g1_i3 from sample A2 / Example spliced isoform

CGCGCGGATCACATGTTACATTTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAGTG
GAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAAAGTAATGAAGTTGA
ATGGGTCAGTGCGTGGTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCAAGCAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGA
ACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTATACCTCTGCATGGGAGACAAAAATTGCATTACCATATGGATGCACTTGCTATGGTTTCATA
GGATTATAAGATTAACAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGC
ATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAA

>TRINITY_DN106488_c0_g1_i5 from sample A2 / Example unspliced isoform:

CGCGCGGATCACATGTTACATTTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAgta
catgtggagtgtccagatatggagggcttgtttcaatatgcagaatccatatgatatggaggagtatccagcacaaaagtcagttttgcagccttttggctttcacaaagttctctatgcaaaatattggttactccatgagatacattcctttcattcatagcttacaccttcagagtttccagcctgctcagcttcac
aaggtagcaagcacttttaagagcagcacctttgggttcccacaatccaagcagcatggcatcagtgaaaagagtgctccagcagctagaaatattgttaataaacattatatgcataccacggcagcaaaagtgggccgaaaaaaatgcagtactcatagatcaaaaataaaggaagctactgatggtccattgaagca
ttctgaaggaaaagatttgagtcagaagccaatagtcatggtgtttgatcttgaaactactggcttaagttgtatcaacgacagaattattgaatttgctgctcaagatcttgttggtggacggcatagtacatttcagtctttggtaaatcctggatgctttgtgacaaatgaaaagattcacggtatcagcaatcaaa
tggtcaataggtctaatgttccaagGTGGAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTT
CCTCTTGCGCGTAAAGTAATGAAGTTGAATGGGTCAGTGCGTGGTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCAAGCAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTT
CAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTATACCTCTGCATGGGAGACAAAAATTGCATTACC
ATATGGATGCACTTGCTATGGTTTCATAGGATTATAAGATTAACAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATAGAATA
CTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAA

Intron 4 GTTCATTTTG ... ATTTTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.91	110	23	25
A2	17.05	73	20	10
A3	19.22	124	35	24
A4	19.77	69	18	16
A5	19.1	36	11	6
A6	20.24	368.5	99	88
A7	18.2	499	118	104
A8	19.34	559	136	132
A9	19.43	479	114	117
A10	19.15	401	116	74

>TRINITY_DN37636_c0_g1_i2 from sample A9 / Example spliced isoform

GTTACATTTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAGTACATGTGGAGTGTCC
AGATATGGAGGGCTTGTTTCAATATGCAGAATCCATATGATATGGAGGAGTATCCAGCACAAAAGTCAGTTTTGCAGCCTTTTGGCTTTCACAAAGTTCTCTATGCAAAATATTGGTTACTCCATGAGATACATTCCTTTCATTCATAGCTTACACCTTCTGAGTTTCCAGCCTGCTCAGCTTCACAAGGTAGCAAGCAC
TTTTAAGAGCAGCACCTTTGGGTTCCCACAATCCAAGCAGCATGGCATCAGCGAAAAGAGTGCTCCAGCAGCTAGAAATATTGTTAATAAACATTATATGCATACCACGGCAGCGAAAGTGGGCCGAAAAAAATGCAGTACTCATAGATCAAAAATAAAGGAAGCTACTGATGGTCCATTGAAGCATTCTGAAGGAAAAG
ATTTGAGTCAGAAGCCAATAGTCATGGTGTTTGATCTTGAAACTACTGGCTTAAGTTGTATCAACGACAGAATTATTGAATTTGCTGCTCAAGATCTTGTTGGTGGACGGCATAGTACATTTCAGTCTTTGGTAAATCCTGGATGCTTTGTGACAAATGAAAAGATTCACGGTATCAGCAATCAAATGGTCAATAGGTCT
AATGTTCCAAGGTGGAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAA
AGTAATGAAGTTGAATGGGTCAGTGCGTGGTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCAAGCAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAAGCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATC
TATTTGAAAATTGAACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTATACCTCTGCATGGGAGACAAAAATTGCATTACCATATGGATGCACTT
GCTATGGTTTCATAGGATTATAAGATTAACAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTG

>TRINITY_DN37636_c0_g1_i4 from sample A9 / Example unspliced isoform:

GTTACATTTTACACTTTACGAGTGTGGAGCCGACATATTAACCCTGAGAATAGGAGAGCAGGACGGAAGGCGGGGGTTTCGGCCCCGTTTCATCAGGCCGATGCATATTTTTGTCACTGTAAACCTCATCAGCCAGAATGTTCGGTCGCCATAACTTGCCCTGCTCGTCATTATCATCTAGAAGTACATGTGGAGTGTCC
AGATATGGAGGGCTTGTTTCAATATGCAGAATCCATATGATATGGAGGAGTATCCAGCACAAAAGTCAGTTTTGCAGCCTTTTGGCTTTCACAAAGTTCTCTATGCAAAATATTGGTTACTCCATGAGATACATTCCTTTCATTCATAGCTTACACCTTCTGAGTTTCCAGCCTGCTCAGCTTCACAAGGTAGCAAGCAC
TTTTAAGAGCAGCACCTTTGGGTTCCCACAATCCAAGCAGCATGGCATCAGCGAAAAGAGTGCTCCAGCAGCTAGAAATATTGTTAATAAACATTATATGCATACCACGGCAGCGAAAGTGGGCCGAAAAAAATGCAGTACTCATAGATCAAAAATAAAGGAAGCTACTGATGGTCCATTGAAGCATTCTGAAGGAAAAG
ATTTGAGTCAGAAGCCAATAGTCATGGTGTTTGATCTTGAAACTACTGGCTTAAGTTGTATCAACGACAGAATTATTGAATTTGCTGCTCAAGATCTTGTTGGTGGACGGCATAGTACATTTCAGTCTTTGGTAAATCCTGGATGCTTTGTGACAAATGAAAAGATTCACGGTATCAGCAATCAAATGGTCAATAGGTCT
AATGTTCCAAGGTGGAAAGATCTTGCTCCTATTTTGGTGCTTTATATAAAGAGTCGACAGAAAGGTGGTGGTCCTGTTATTTGGGTGGCTCATAATGGTCGCCGTTTTGATGCACGTTTTCTTGTGAAGGAGTTTAATCGCTGTTCACTGGAAATTCCATCTGATTGGGTTTTTGTAGATACACTTCCTCTTGCGCGTAA
AGTAATGAAGTTGAATGgttcattttgataggaagatcaaaccctaggggttggacaagtctcccagctcatctccaatgtcatttggggtgatcctgggtggttttgaagtgccaaatccatctttcaagcccttgctgcagaaaattatagtcctgaaggtgaaatcagtcccaaaacaaagcaatcatccatcagaa
gaagtgcgccctagggagggttgagtctcctcgggttgttgatcgtcgaatggtatttttcaagGGTCAGTGCGTGGTAATACAAACTTAACTGCCCTTCGTGAGCACTATCAAATTCCATTAACAGGAGTAGCACACCAAGCAATGTCAGATGTCAATACACTTGCATTAGTGCTGCAAAAATTATCATTTGACCTAAA
GCTACCTGTTTCAGGTCTTCTAGAAATGTCTTTCAAGGCTTCAGATCTATTTGAAAATTGAACTTTTGTAACAATAGAATACTATTTGAAATTCCATTAGATCATTGGCATCCACGAATGCATGAAAACCTGAGGTTATTTTAATATTTATCTACACAGGAGACCGAAATGTCATAATCCACATGAATTGCCTTCCTGTA
TACCTCTGCATGGGAGACAAAAATTGCATTACCATATGGATGCACTTGCTATGGTTTCATAGGATTATAAGATTAACAGGAGTAGCACACCGAGTAATGTCAGATGTCAATACACTTG