sp|Q9FHM7|HIR4_ARATH ----> Hypersensitive-induced response protein 4

Intron 1 GTTTGGCCAC ... ATGGTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.34	137	14.5	49
A2	19.39	133	18	32
A3	20.97	147	14	39
A4	13.33	78	9	12
A5	11.11	40	7	5
A6	18.94	552	42	129
A7	15.34	839	65.5	152
A8	17.27	752	82	157
A9	15.1	652	64	116
A10	14.77	508	54	88

>TRINITY_DN115034_c0_g1_i2 from sample A1 / Example spliced isoform

TTTCGATATCCACGATTAAATTATATTAAATAATTTGGTAATTTGGTCTCCTCTCTACTGAAGTTATCTTGCTCCACCTGCACTAGAAATACACCACCTGTACTAGAAATTCAGATCTGTGTTTGCACGTAAGCAAATTGCACACCATATTTTAGGTTTTTAGAGATCTGCAGGGAATTTTAGGTGGCCAGTTGAGAGAA
ACCTCAAACTTTGAAGGTGGGTTGGAGGAGGAAATGGGGGGTTTCATGTTTTGGGTGTGCATCGGGCAGGCGAATGTTGGGATTATTGAGAAGTGGGGTCGTTTCTTGAAGCTTGCAGAGCCGGGTTTGCATTTCGTCAATCCTTGTTTTGGTGAATGGCTGGCTGGCACTCTCTCTACTCGCCTCCAATACCTCGACGT
CCGTGTCGAGACCAAAACAAAGGACAATGTCTTTGTGCAATTATTTTGCTCCATCCAGTATCGTGTTGTGAAACAGGATGCTGACGATGCATTTTATGAGCTGCAAAATCCTCAAGAACAAATTCAAGCTTATGTGTTTGATGTGGTTCGAGCAAATGTTCCAAAGATGAACTTGGATGAATTATTTGAGCAAAAGGGCG
ATGTTGCCAAAGTTGTGTTGGAAGAGCTTGAAAAAGCGATGGGATCATATGGGTTTAACATACAGCAGATACTTGTGGTTGATATAGTTCCGGATGTTTCAGTACGGAGGGCAATGAATGAGATCAATGCAGCTCAACGTCTTCAACTTGCAAGTGTGTATAGAGGTGAAGCAGAGAAAATACTACAAGTTAAAAAGGCA
GAAGGTGATGCAGAGGCAAAATATCTTGCTGGTGTTGGGGTTGCACGACAGAGACAAGCTATCACAGATGGGCTACGAGAAAATGTGCTAGAATTTTCCCATAAAGTCCCTGGCACTTCTTCTAAGGATGTCATGGATCTAGTCATGATCACTCAGTACTTTGACACCATAAAAGATGTTGGGGCATCATCCAAGAACAC
GACTGTCTTCATTCCGCATGGTCCAGGGCATGTGAGAGACGTCAGTGAGCAAATAAGGAATGGACTTATGCAAGCATCAAGTATTTCTGAGGATTTACATTAAAGAACATTAGGAAGGATTTGATTCTGCTTTAATGCATGAAATATGCTTGGTTCCTTTACTTCCTCTGTGAAAACAGGGAAACTTTTATTATAAACAC
GAGTACAGGTGCTGAGGTGAATAAATAAAGTGTATATGTCTTTCATTTCAAATTCCGAGCGTTCAGCTT

>TRINITY_DN115034_c0_g1_i3 from sample A1 / Example unspliced isoform:

CTAACTGATATTTGTGGATGTAAATTTTATGATCAGGCGATGGGATCATATGGGTTTAACATACAGCAGATACTTGTGGTTGATATAGTTCCGGATGTTTCAGTACGGAGGGCAATGAATGAGATCAATGCAGgtttggccacctttatccacctttttttttgaagagaagcattaagattgctcaagtgttattgcat
ttaatatttattcatttgaggtgtcttgccggtttcaactttcatatgtatagtggatactgttggaccaaacttggtagtgataacatcattgtaaatgattttgtgcatacatcttccataatcaagtggcatttattaactattagatttgagtgattcatggtcgcagCTCAACGTCTTCAACTTGCAAGTGTGTA
TAGAGGTGAAGCAGAGAAAATACTACAAGTTAAAAAGGCAGAAGGTGATGCAGAGGCAAAATATCTTGCTGGTGTTGGGGTTGCACGACAGAGACAAGCTATCACAGATGGGCTACGAGAAAATGTGCTAGAATTTTCCCATAAAGTCCCTGGCACTTCTTCTAAGGATGTCATGGATCTAGTCATGATCACTCAGTACT
TTGACACCATAAAAGATGTTGGGGCATCATCCAAGAACACGACTGTCTTCATTCCGCATGGTCCAGGGCATGTGAGAGACGTCAGTGAGCAAATAAGGAATGGACTTATGCAAGCATCAAGTATTTCTGAGGATTTACATTAAAGAACATTAGGAAGGATTTGATTCTGCTTTAATGCATGAAATATGCTTGGTTCCTTT
ACTTCCTCTGTGAAAACAGGGAAACTTTTATTATAAACACGAGTACAGGTGCTGAGGTGAATAAATAAAGTGTATATGTCTTTCATTTCAAATTCCGAGCGTTCAGCTT

Intron 2 GTTTGGCCAC ... ATGGTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.34	137	2	49
A2	19.39	133	1	32
A3	20.97	147	3	39
A4	13.33	78	0	12
A5	11.11	40	3	5
A6	18.94	552	0	129
A7	15.34	839	0	152
A8	17.27	752	3	157
A9	15.1	652	0	116
A10	14.77	508	1	88

>TRINITY_DN34944_c0_g1_i1 from sample A9 / Example spliced isoform

CATTTGGTAATTTGGTCTCCTCTCTACTGAAGTTATCTTGCTCCACCTGCACTAGAAATACACCACCTGTACTAGAAATTCATCACCGTCTTCATGTTTCGATATCCACGATTAAATTATATTAAATCATTTGGTAATTTGGTCTCCTCTCTACTGAAGTTATCTTGCTCCACCTGCACTAGAAATACACCACCTGTACT
AGAAATTCAGATCTGTGTTTGCACGTAAGCAAATTGCACACCATATTTTAGGTTTTTAGAGATCTGCAGGGAATTTTAGGTGGCCAGTTGAGAGAAACCTCAAACTTTGAAGGTGGGTTGGAGGAGGAAATGGGGGGTTTCATGTTTTGGGTGTGCATCGGGCAGGCGAATGTTGGGATTATTGAGAAGTGGGGTCGTTT
CTTGAAGCTTGCAGAGCCGGGTTTGCATTTCGTCAATCCTTGTTTTGGTGAATGGCTGGCTGGCACTCTCTCTACTCGCCTCCAATACCTCGACGTCCGTGTCGAGACCAAAACAAAGGACAATGTCTTTGTGCAATTATTTTGCTCCATCCAGTATCGTGTTGTGAAACAGGATGCTGACGATGCATTTTATGAGCTGC
AAAATCCTCAAGAACAAATTCAAGCTTATGTGTTTGATGTGGTTCGAGCAAATGTTCCAAAGATGAACTTGGATGAATTATTTGAGCAAAAGGGCGATGTTGCCAAAGTTGTGTTGGAAGAGCTTGAAAAAGCGATGGGATCATATGGGTTTAACATACAGCAGATACTTGTGGTTGATATAGTTCCGGATGTTTCAGTA
CGGAGGGCAATGAATGAGATCAATGCAGCTCAACGTCTTCAACTTGCAAGTGTGTATAGAGGTGAAGCAGAGAAAATACTACAAGTTAAAAAGGCAGAAGGTGATGCAGAGGCAAAATATCTTGCTGGTGTTGGGGTTGCACGACAGAGACAAGCTATCACAGATGGGCTACGAGAAAATGTGCTAGAATTTTCCCATAA
AGTCCCTGGCACTTCTTCTAAGGATGTCATGGATCTAGTCATGATCACTCAGTACTTTGACACCATAAAAGATGTTGGGGCATCATCCAAGAACACGACTGTCTTCATTCCGCATGGTCCAGGGCATGTGAGAGACGTCAGTGAGCAAATAAGGAATGGACTTATGCAAGCATCAAGTATTTCTGAGGATTTACATTAAA
GAACATTAGGAAGGATTTGATTCTGCTTTAATGCATGAAATATGCTTGGTTCCTTTACTTCCTCTGTGAAAACAGGGAAACTTTTATTATAAACACGAGTACAGGTGCTGAGGTGAATAAATAAAGTGTATATGTCTTTCATTTCAAATTCCGAGCGTTCAGCTTTCACTTTTTAATAGTTTTATGTACATGC

>TRINITY_DN34944_c0_g1_i2 from sample A9 / Example unspliced isoform:

TGTTTCAGTACGGAGGGCAATGATGAGATCAATGCAGgtttggccacctttatccaccttttttttttgaagagaagcattaagattgctcaagtgttattgcatttaatatttattcatttgaggtgtcttgccggtttcaactttcatatgtatagtggatactgttggaccaaacttggtagtgataacatcattgt
aaatgattttgtgcatacatcttccataatcaagtggcatttattaactattagatttgagtgattcatggtcgcagCTCAACGTCTTCAACTTGCAAGTGTGTATAGAGGTGAAGCAGAGAAAATACTACAAGTTAAAAAGGCAGAAGGTGATGCAGAGGCAAAATATCTTGCTGGTGTTGGGGTTGCACGACAGAGAC
AAGCTATCACAGATGGGCTACGAGAAAATGTGCTAGAATTTTCCCATAAAGTCCCTGGCACTTCTTCTAAGGATGTCATGGATCTAGTCATGATCACTCAGTACTTTGACACCATAAAAGATGTTGGGGCATCATCCAAGAACACGACTGTCTTCATTCCGCATGGTCCAGGGCATGTGAGAGACGTCAGTGAGCAAATA
AGGAATGGACTTATGCAAGCATCAAGTATTTCTGAGGATTTACATTAAAGAACATTAGGAAGGATTTGATTCTGCTTTAATGCATGAAATATGCTTGGTTCCTTTACTTCCTCTGTGAAAACAGGGAAACTTTTATTATAAACACGAGTACAGGTGCTGAGGTGAATAAATAAAGTGTATATGTCTTTCATTTCAAATTC
CGAGCGTTCAGCTTTCACTTTTTAATAGTTTTATGTACATGC