sp|O48538|RBOHF_ARATH ----> Respiratory burst oxidase homolog protein F

Intron 1 GTGAGATAAT ... AATTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.93	6.5	9	7.3
A2	22.66	14.9	7	1.6
A3	28.42	25.4	9	9.7
A4	24.41	20.1	9	3.7
A5	31.33	30.5	15	12.5
A6	23.64	1.7	1	0
A7	43.41	4.7	4	3.3
A8	34.2	3.9	2	1.3
A9	26.32	7	5	0
A10	40.03	11.6	10	5.5

>TRINITY_DN131744_c2_g1_i9 from sample A1 / Example spliced isoform

CCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCT
TACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGC
CGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCT
CGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAA
TGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGT

>TRINITY_DN131744_c2_g1_i17 from sample A1 / Example unspliced isoform:

CCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCT
TACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGC
CGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCT
CGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATgtgagataattc
ggctatttttagcagcgcggatgtagattcaagatttaagtatctttcccttcagacgctaaaatttggacgactcgtgctggccttgtaattttatcgggaattttgctattaagtctttgacattgtcacttagctttcatctttaaatgttctgagcgagagttccttttactgtatctggtttgcttgttgcttaa
gctttttcagagtactgaactcgtacattttgttttgatttaaattgtgcagGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCAT
TTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCAT
ATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTG
TGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTA
GGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCG
AGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTC
TCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAAT
CACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGC
TACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGC
ACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTC
TATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATT
TCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT

Intron 2 GTAAGTCCAG ... TTGAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.36	41	14	11
A2	28.57	10	2	6
A3	16.67	10	0	4
A4	38.46	12	6	9
A5	20	24	8	4
A6	18.87	43	7	13
A7	20	48	12	12
A8	19.19	40	10	9
A9	21.74	45	8	17
A10	38.1	26	20	12

>TRINITY_DN43596_c0_g1_i10 from sample A7 / Example spliced isoform

GCCTCAGCTTCGTCTCAGGATTGATTCTGGCTGTGAATCCCTGTAGTCCCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGA
AGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTA
GGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTC
GATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCA
AAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGC
TAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATG
TTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAA
CATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTT
TTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAA
TCAGTAAGGACATTTCAAGTTGTTTTCTGTTAATTTCAAACCACATGTCTAACTTCCATTTAAAACGCCAGAATATTTCTAATTTTGTTATAAAGTGCTTAAGAATTTAGCTTAAACGAAAATAAACAAACACGATTTGCTCTAGGAATTTGATGATGTTTATCCACCGAGAAAGTTCAGGGCTTGAAATGATTTGGATG
TGTAGACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAG
ATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAG
CAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGA
ACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGAT
ATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAA
TTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACA
TGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTAC
AATTTGCACTGTTTGAACAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTTGAAAATAAACGTACGATATAA
TCTTATTCCAACGACGTGCGCTCACGTTCATTGTTTTATTTCCTGTATACATGGGAAATTGGAACCTCATCTTATATTCGTGGGCACGTATAATCAGCAAATAGATACAAGCAGACAAATGGTAGGACCTGGGCCTACCCACGCCTTCGCTTGTCTCCCTCTTGGCCCGGAATTGTATAA

>TRINITY_DN43596_c0_g1_i3 from sample A7 / Example unspliced isoform:

TTGGACGACTCGTGCTGGCCTTGTAATTTTATCGGGAATTTTGCTATTAAGTCTTTGACGTTGTCACTTAGCTTTCATCTTTAAATGTTCTGAGCGAGAGTTCCTTTTACTGTATCTGGTTTGCTTGTTGCTTAAGCTTTTTCAGAGTACTGAACTCGTACATTTTGTTTTGATTTAAATTGTGCAGGTGTGATAAGAAT
GCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGG
CCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCT
TCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATG
CTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTT
TAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAGTAAGGACATTTCAAGTTGTTTTCTGTTAATTTCAAACCACATGTC
TAACTTCCATTTAAAACGCCAGAATATTTCTAATTTTGTTATAAAGTGCTTAAGAATTTAGCTTAAACGAAAATAAACAAACACGATTTGCTCTAGGAATTTGATGATGTTTATCCACCGAGAAAGTTCAGGGCTTGAAATGATTTGGATGTGTAGACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGA
GCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATG
ATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAG
CGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTATGTTAAAGATAACTGACCAAATCAAAACCTCAGATATCCAAGATTCAAAGTATTTAACAAAACGAACCCTGTGCAGGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCG
GGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAG
GCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGC
TAGAATTGgtaagtccagccttcaaaatttcaggcgaattatggatcttctgaaatcggccctttcttatattaatgttagcttgaatgcagGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCT
AACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACATGAAT
GGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTACAATTT
GCACTGTTTGAACAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTTGAAAATAAACGTACGATATAATCTTA
TTCCAACGACGTGCGCTCACGTTCATTGTTTTATTTCCTGTATACATGGGAAATTGGAACCTCATCTTATATTCGTGGGCACGTATAATCAGCAAATAGATACAAGCAGACAAATGGTAGGACCTGGGCCTACCCACGCCTTCGCTTGTCTCCCTCTTGGCCCGGAATTGTATAA

Intron 3 GTATTTTAAT ... ATTGGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.41	18	11	4
A2	29.87	27	14	9
A3	33.33	20	18	2
A4	26.67	11	5	3
A5	26.09	17	8	4
A6	12.5	7	2	0
A7	0	1	0	0
A8	20	4	1	1
A9	20	4	1	1
A10	15.38	11	3	1

>TRINITY_DN131744_c2_g1_i1 from sample A1 / Example spliced isoform

TTTGGTGAATTTGACTAAGGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGA
AAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACAT
GGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCG
AATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACT
TGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGT
TCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTT
CTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTATGTTAAAGATAACTGACCAAATCAAAACCTCAGATATCCAAGATTCAAAGTATTTAACAAAACGAACCCTGTGCAGGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGT
TACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAG
ATGGACCGCAAGGTAAAATTTATCTCATCGCCCAGTCTGCAAGTTCCAAAATTTTGTCGAATTGAAAACGGATTAACCCA

>TRINITY_DN131744_c2_g1_i6 from sample A1 / Example unspliced isoform:

CCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCT
TACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGC
CGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCT
CGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGAGATAATTC
GGCTATTTTTAGCAGCGCGGATGTAGATTCAAGATTTAAGTATCTTTCCCTTCAGACGCTAAAATTTGGACGACTCGTGCTGGCCTTGTAATTTTATCGGGAATTTTGCTATTAAGTCTTTGACATTGTCACTTAGCTTTCATCTTTAAATGTTCTGAGCGAGAGTTCCTTTTACTGTATCTGGTTTGCTTGTTGCTTAA
GCTTTTTCAGAGTACTGAACTCGTACATTTTGTTTTGATTTAAATTGTGCAGGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCAT
TTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCAT
ATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTG
TGCCATTTGACGACAACATCAATTTCCACAAGgtattttaattctcataaagctaaacaggaaatggatttcgaagttctttattaacacttcaattttatattggtgcagACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGA
GGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACG
CTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCA
GTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAG
AGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGA
TCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGG
GAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAAC
TCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACA
TTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT

Intron 4 GCAAGCGGTT ... GTATCCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60	8	12	3
A2	66.67	4	8	1
A3	66.67	5	10	4
A4	61.54	5	8	2
A5	73.33	4	11	3
A6	70.21	14	33	9
A7	63.69	13	22.8	7
A8	68.63	16	35	5
A9	62.22	17	28	4
A10	70	6	14	7

>TRINITY_DN41952_c1_g1_i2 from sample A6 / Example spliced isoform

CCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTC
GGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAG
CACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGAT
GCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAA
GCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCA
TACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATG
GCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTA
TAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGT
ATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGC
GGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTC
TCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCA
TAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTT
ACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCC
TCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTTGAAAAT
AAACGTACGATATAATCTTATTCCAACGACGTGCGCTCACGTTCATTGTTTTATTTCCTGTATACATGGGAAATTGGAACCTCATCTTATATTCGTGGGCACGTATAATCAGCAAATAGATACAAGCAGACAAATGGTAGGACCTGGGCCTACCCACGCCTTCGCTTGTCTCCCTCTTGGCCCGGAATTGTATAAGGACT
AACCAAAACGTCTTGATTTTGGTAGAGCAATTTTTGTCAAAATTATTATTTAGAGTATTCAGATCACAATTTGGATGATCTGGGTAGTTTTAAAATTGGT

>TRINITY_DN41952_c1_g1_i1 from sample A6 / Example unspliced isoform:

CCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTC
GGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAG
CACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGAT
GCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAA
GCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCA
TACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATG
GCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTA
TAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGT
ATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGC
GGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTC
TCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCA
TAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTT
ACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCC
TCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGgcaagcggtttacattatttcaactctggatcagatttctacattattaacttagagtgctgttgtattctaatttctggaaaagatcagatttctacattatt
aatttagagtgctgttgtatcctaatttctggaaaaagaaaaggtaagatttaatttcatagactgccatttcatagctggacatgaatggaaacatgcagctcacaccttcttggcttttcccttagacagtaagggaaacagtgatgtaactttggctgaataggctccagcattagagacaagtccatatttcaacc
attacccattgtcacgaacaagaaataatagatatatttattgtagatagctggaaatggtattgtaaaatatattgctcctacaatttgcactgtttgaacaggggtggtagactggtagactccattcaatttgaaatattttttgaatcctcgtatccgcagTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAAC
TTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTTGAAAATAAACGTACGATATAATCTTATTCCAACGACGTGCGCTCACGTTCATTGTTTTATTTCCTGTATACATGGGAAATTGGAACCTCATCTTATATTCGTGGGCACGTATAATCAGCAAATAGATACAAGCAGAC
AAATGGTAGGACCTGGGCCTACCCACGCCTTCGCTTGTCTCCCTCTTGGCCCGGAATTGTATAAGGACTAACCAAAACGTCTTGATTTTGGTAGAGCAATTTTTGTCAAAATTATTATTTAGAGTATTCAGATCACAATTTGGATGATCTGGGTAGTTTTAAAATTGGT

Intron 5 GTCAACTTTG ... TACCTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.39	31	8	12
A2	32.26	21	8	12
A3	13.21	23	3	4
A4	30.61	17	10	5
A5	20.69	23	1	11
A6	20	4	0	2
A7	12.82	17	3	2
A8	27.27	4	0	3
A9	30.77	9	2	6
A10	25	3	1	1

>TRINITY_DN99118_c0_g1_i7 from sample A4 / Example spliced isoform

CGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGG
AGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCT
GTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAAT
TTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATG
CTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGT
TCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGG
AAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCA
AATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAAC
CGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTG
TCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAG
GAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGG
AATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCA
TAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATT
GGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAA
GATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGA
GACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTACAATTTGCACTGTTTGAAAAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTTTCGAG
TTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTT

>TRINITY_DN99118_c0_g1_i6 from sample A4 / Example unspliced isoform:

GGACGAAGTAAAAGAGGTAAATCTTTTCCTAAACATGTAATTTCTTTGAAATGCATTGAAGGTATGGATTTTTATGTAAAGCATACTGAAAATTGCTAAATAAATCAGTAATTTAATCGAATATTATATCCTAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCA
AATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGgtcaactttgctatctgcaaatcaaattttcaagcaaatttatctattattattatatttttatttatagatgacctcgtatcgctaatttctgctcaatttgatcaagaaactgaaaaccttgcttataatgctttggtgaatttgactaa
ggttttaaatttcatattacctttcagCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTG
GGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCT
TAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTA
GGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCT
TTTCTTCGTGCGAGATTGGAGGGAAAAATCAGTAAGGACATTTCAAGTTGTTTTCTGTTAATTTCAAACCACATGTCTAACTTCCATTTAAAACGCCAGAAAATTTCTAATTTTGTTATAAAGTGCTTAAGAATTTAGCTTAAACGAAAATAAACAAACACGATTTGCTCTAGGAATTTGATGATGTTTATCCACCGAGA
AAGTTCAGGGCTTGAAATGATTTGGATGTGTAGACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGG
AATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGTAAGTTTTGAATATACGTTAGAATTTATAAAATTGTATGATCTTTTAGGGTTTCTTCCACAACTCTTACATTTTGTTATGAACTTTCTTGATACAGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGA
ACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGA
CCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCC
CGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGC
GTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAA
AGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGC
TGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGC
TCCTACAATTTGCACTGTTTGAAAAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTTTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTT

Intron 6 GTGATAACTG ... ATCTTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.97	10.7	6.5	3.4
A2	24.98	17	12.3	5.9
A3	15.26	15.1	1.8	2.9
A4	20.77	22.9	11.5	6.4
A5	31.2	14	8.2	6.5
A6	22.04	28.7	9.4	8.6
A7	19.68	41	14	10.7
A8	21.83	9.7	5.7	2.8
A9	17.79	21.1	2.7	5.3
A10	16.39	13.7	3.6	3.8

>TRINITY_DN131744_c2_g1_i1 from sample A1 / Example spliced isoform

TTTGGTGAATTTGACTAAGGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGA
AAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACAT
GGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCG
AATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACT
TGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGT
TCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTT
CTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTATGTTAAAGATAACTGACCAAATCAAAACCTCAGATATCCAAGATTCAAAGTATTTAACAAAACGAACCCTGTGCAGGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGT
TACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAG
ATGGACCGCAAGGTAAAATTTATCTCATCGCCCAGTCTGCAAGTTCCAAAATTTTGTCGAATTGAAAACGGATTAACCCA

>TRINITY_DN131744_c2_g1_i6 from sample A1 / Example unspliced isoform:

CCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCT
TACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGC
CGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCT
CGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGAGATAATTC
GGCTATTTTTAGCAGCGCGGATGTAGATTCAAGATTTAAGTATCTTTCCCTTCAGACGCTAAAATTTGGACGACTCGTGCTGGCCTTGTAATTTTATCGGGAATTTTGCTATTAAGTCTTTGACATTGTCACTTAGCTTTCATCTTTAAATGTTCTGAGCGAGAGTTCCTTTTACTGTATCTGGTTTGCTTGTTGCTTAA
GCTTTTTCAGAGTACTGAACTCGTACATTTTGTTTTGATTTAAATTGTGCAGGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCAT
TTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCAT
ATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTG
TGCCATTTGACGACAACATCAATTTCCACAAGGTATTTTAATTCTCATAAAGCTAAACAGGAAATGGATTTCGAAGTTCTTTATTAACACTTCAATTTTATATTGGTGCAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGA
GGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACG
CTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCA
GTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAG
AGTTTTCTCTCAGgtgataactgctttagtattttaagcttcagtactatgaagtttcctgtaggctgtaaattctaatcacagatctttatagACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGA
TCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGG
GAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAAC
TCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACA
TTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT

Intron 7 GTACATATTT ... TGGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.4	26.1	3	6.7
A2	26.67	11	4	1
A3	47.66	9.9	9	0.6
A4	25.4	17.7	6	1.7
A5	43.87	16.7	13	6.1
A6	29.5	45.7	19	10.7
A7	15.69	27.1	5	7.9
A8	19.9	32.7	8	6.7
A9	4.08	24	1	5
A10	21.04	41.3	11	3.3

>TRINITY_DN103750_c0_g1_i3 from sample A5 / Example spliced isoform

GATTGATTCTGGCTGTGAATCCCTGTAGTCCCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGG
CGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTA
GTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAG
CTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCT
GCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTC
TCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGG
AAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGAC
CATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGG
CTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATT
GCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCA
TCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAG
CTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTATGTTAAAGATAACTGACCAAATCAAAACCTCAGATATCCAAGATTCAAAGTATTTAACAAAACGAACCCTGTGCAGGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTA
CAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCA
TGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTT
TCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATT
AACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAG
TGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTACAATTTGCACTGTTTGAACAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTT
TTTGAATCCTCGTATCCGCAGTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATG

>TRINITY_DN103750_c0_g1_i2 from sample A5 / Example unspliced isoform:

GATTGATTCTGGCTGTGAATCCCTGTAGTCCCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGG
CGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTA
GTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAG
CTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCT
GCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTC
TCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGG
AAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGAC
CATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGG
CTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATT
GCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCA
TCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGC
TTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTC
ACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGgt
acatattttatttatttatttttttcattattgctccaattttacattatctgagggccatagaaatgcattgtttgaactttgaagtccactggtttgcagGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCC
TCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATT
AATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACC
ATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTACAATTTGCACTGTTTGAACAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAAC
TTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATG

Intron 8 GTCATGCAGT ... CATTTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	6	6	3
A2	20.14	14.9	4	0
A3	28.96	11.6	4.8	1
A4	0	23	0	0
A5	42.34	18.8	13.8	6
A6	10	9	1	0
A7	28.07	14	5.5	0
A8	11.11	8	1	0
A9	12.23	19.6	2.8	1
A10	25.35	10.9	4	1

>TRINITY_DN103750_c0_g1_i3 from sample A5 / Example spliced isoform

GATTGATTCTGGCTGTGAATCCCTGTAGTCCCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGG
CGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTA
GTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAG
CTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCT
GCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTC
TCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGG
AAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGAC
CATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGG
CTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATT
GCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCA
TCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAG
CTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTATGTTAAAGATAACTGACCAAATCAAAACCTCAGATATCCAAGATTCAAAGTATTTAACAAAACGAACCCTGTGCAGGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTA
CAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCA
TGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTT
TCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATT
AACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAG
TGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTACAATTTGCACTGTTTGAACAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTT
TTTGAATCCTCGTATCCGCAGTTGAAAGGTCTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATG

>TRINITY_DN103750_c0_g1_i4 from sample A5 / Example unspliced isoform:

TATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATAT
TACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGA
AGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAG
AATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATA
AACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTC
AATATTGTCAAGgtcatgcagtttctatttaaatttgcatttttctcgtatcatctgtttcttgaaatccctttcatgtgcaatcaacatatctgccttattcatttcgcagGCCGCAGTTTATCGCGGAAAC

Intron 9 GTAAGGACAT ... GGATGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.03	17	16	13
A2	11.11	28	6	1
A3	26.32	14	6	4
A4	30.23	30	16	10
A5	39.73	22	12	17
A6	42.86	4	0	6
A7	36.17	15	7	10
A8	25	3	1	1
A9	45.45	6	6	4
A10	25.93	10	6	1

>TRINITY_DN131744_c2_g1_i1 from sample A1 / Example spliced isoform

TTTGGTGAATTTGACTAAGGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGA
AAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACAT
GGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCG
AATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACT
TGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGT
TCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTT
CTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTATGTTAAAGATAACTGACCAAATCAAAACCTCAGATATCCAAGATTCAAAGTATTTAACAAAACGAACCCTGTGCAGGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGT
TACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAG
ATGGACCGCAAGGTAAAATTTATCTCATCGCCCAGTCTGCAAGTTCCAAAATTTTGTCGAATTGAAAACGGATTAACCCA

>TRINITY_DN131744_c2_g1_i13 from sample A1 / Example unspliced isoform:

TTTGGTGAATTTGACTAAGGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGA
AAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACAT
GGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCG
AATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACT
TGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAgtaaggacatttcaagttgttttctgttaatttcaaaccacatgtctaacttccatttaaaacgccagaaaatttctaattttgttataaagtgcttaagaatttagcttaaacgaaaataaacaaacacgatttgctctaggaatttgat
gatgtttatccaccgagaaagttcagggcttgaaatgatttggatgtgtagACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGG
GTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTAC
TATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAAC
GTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGC
AGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTT
CTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCT
GTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT

Intron 10 GTAAGATTTT ... TGTTCGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.34	26	1.8	3
A2	20.75	42	4.8	11
A3	21.43	22	2.2	6
A4	28	36	4	14
A5	16.36	46	5	9
A6	-1	0	0	0
A7	13.33	13	0.8	2
A8	50	2	0	2
A9	14.29	6	0.8	1
A10	0	5	0.4	0

>TRINITY_DN107905_c0_g1_i1 from sample A2 / Example spliced isoform

TCTCAGGATTGATTCTGGCTGTGAATCCCTGTAGTCCCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCG
CGCGGGCGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTT
CGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTC
GATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTC
TCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTAAATCTTTTCCTAAACATGTAATTTCTTTGAAATGCATTGAAGGTATGGATTTTTATGTAAAGCATACTGAAAATTGCTAAATAAATCAGTAATTTAATCGAAT

>TRINITY_DN107905_c0_g1_i3 from sample A2 / Example unspliced isoform:

CCTTTGCATTGgtaagatttttgagtcattggatgttgatttggattttacatagattacctcggttcgaatctcagcgtttgtaaaatgcgttgtttgttcggcagGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGC
AGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTAAATCTTTTCCTAAACATGTAATTTCTTTGAAATGCATTGAAGGTATGGATTTTTATGTAAAGCATACTGAAAATTGCT
AAATAAATCAGTAATTTAATCGAAT

Intron 11 GTAAGTTTTG ... CTTGATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	12	6	6
A2	16.13	26	7	3
A3	15.25	25	5	4
A4	23.81	24	10	5
A5	22.58	24	10	4
A6	15.25	25	7	2
A7	3.45	42	3	0
A8	5.56	17	2	0
A9	9.52	19	4	0
A10	8.33	22	4	0

>TRINITY_DN99118_c0_g1_i7 from sample A4 / Example spliced isoform

CGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCTTACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGG
AGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGCCGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCT
GTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCTCGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAAT
TTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATG
CTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGT
TCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGG
AAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCA
AATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAAC
CGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTG
TCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAG
GAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGG
AATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCA
TAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATT
GGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAA
GATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGCTGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGA
GACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGCTCCTACAATTTGCACTGTTTGAAAAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTTTCGAG
TTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTT

>TRINITY_DN99118_c0_g1_i6 from sample A4 / Example unspliced isoform:

GGACGAAGTAAAAGAGGTAAATCTTTTCCTAAACATGTAATTTCTTTGAAATGCATTGAAGGTATGGATTTTTATGTAAAGCATACTGAAAATTGCTAAATAAATCAGTAATTTAATCGAATATTATATCCTAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCA
AATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGGTCAACTTTGCTATCTGCAAATCAAATTTTCAAGCAAATTTATCTATTATTATTATATTTTTATTTATAGATGACCTCGTATCGCTAATTTCTGCTCAATTTGATCAAGAAACTGAAAACCTTGCTTATAATGCTTTGGTGAATTTGACTAA
GGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTG
GGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCT
TAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTA
GGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCT
TTTCTTCGTGCGAGATTGGAGGGAAAAATCAGTAAGGACATTTCAAGTTGTTTTCTGTTAATTTCAAACCACATGTCTAACTTCCATTTAAAACGCCAGAAAATTTCTAATTTTGTTATAAAGTGCTTAAGAATTTAGCTTAAACGAAAATAAACAAACACGATTTGCTCTAGGAATTTGATGATGTTTATCCACCGAGA
AAGTTCAGGGCTTGAAATGATTTGGATGTGTAGACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGG
AATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGgtaagttttgaatatacgttagaatttataaaattgtatgatcttttagggtttcttccacaactcttacattttgttatgaactttcttgatacagGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGA
ACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGA
CCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCC
CGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGC
GTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAA
AGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCATAGC
TGGACATGAATGGAAACATGCAGCTCACACCTTCTTGGCTTTTCCCTTAGACAGTAAGGGAAACAGTGATGTAACTTTGGCTGAATAGGCTCCAGCATTAGAGACAAGTCCATATTTCAACCATTACCCATTGTCACGAACAAGAAATAATAGATATATTTATTGTAGATAGCTGGAAATGGTATTGTAAAATATATTGC
TCCTACAATTTGCACTGTTTGAAAAGGGGTGGTAGACTGGTAGACTCCATTCAATTTGAAATATTTTTTGAATCCTCGTATCCGCAGTTGAAAGGTTTCGAGTTCGAATTCTAATAGCGAACTTGGTCAGCCGAATATAGCTGTGTGCGCACATTGCAAGTATATCCTTTACATGGGAAATCGTT

Intron 12 GTAAATCTTT ... TATATCCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.89	12.7	3	5.7
A2	25.57	13	1.3	4.7
A3	15.94	36.3	2.7	7.3
A4	42.82	17	6	11.7
A5	20.29	28.7	9.3	7.3
A6	31.31	3.3	0	1.7
A7	26.32	12.3	0.7	4.7
A8	5.42	5	0	0.3
A9	32.52	5.3	0	2.7
A10	9.76	9.3	0.7	1

>TRINITY_DN131744_c2_g1_i17 from sample A1 / Example spliced isoform

CCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCT
TACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGC
CGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCT
CGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGAGATAATTC
GGCTATTTTTAGCAGCGCGGATGTAGATTCAAGATTTAAGTATCTTTCCCTTCAGACGCTAAAATTTGGACGACTCGTGCTGGCCTTGTAATTTTATCGGGAATTTTGCTATTAAGTCTTTGACATTGTCACTTAGCTTTCATCTTTAAATGTTCTGAGCGAGAGTTCCTTTTACTGTATCTGGTTTGCTTGTTGCTTAA
GCTTTTTCAGAGTACTGAACTCGTACATTTTGTTTTGATTTAAATTGTGCAGGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCAT
TTGGGTTACATTGAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGAAAATTGGCAAAGGGCCTGGGTTATTGCCAT
ATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACATGGCTTCGCTCCACGATCTTAGGATCCATTG
TGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCGAATTACATAGATATCTTAGGAAGCACAGTA
GGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACTTGTTCATTCCATTCACCTTTTCTTCGTGCG
AGATTGGAGGGAAAAATCAACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGGGTTCGAGTACAAGAGCGGAATGTATTTGTTTC
TCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTACTATGAAGTTTCCTGTAGGCTGTAAATTCTAAT
CACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGTGTTACTCCTGATCGGGCTCGGAATCGGCGC
TACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAGAGATGGACCGCAAGGCCACCATAGAAATGC
ACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCTTCTAATAGAGATGCTAGAATTGGGGTGTTC
TATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGTTGTATTCTAATTTCTGGAAAAGATCAGATT
TCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT

>TRINITY_DN131744_c2_g1_i8 from sample A1 / Example unspliced isoform:

CCTGGATCGAATCACGTAACGACGGAATTAAAACCAGAAGCCATGACGCTCGGTATGGGCAGAAACCCCGAGGACCTCATAAACTCTTTTGCTCTCAAGTTGGGAATGGACATCGATAATAACGGCGAGAATGACCTTTCGAAGCTCCAGGAAGTAATGACGCGCGCGGGCGAACAAAACGATGAGCATTACATCGAGCT
TACGGTGGACGAACATGACGAGTCTGATATTTTGGTGGGAGAATGGAGCAGGAAGGGCTATTTGTCGGCCAAAATGAGGCAATTGTCGCACGAGCTGAGAGCGGATTTCAGGCCAGTGAAGCATTTGTCTCATGAATTGAAGCGGATCACTAGGACGGCCAGTTCGATTAGTGCCAAGGTTTTGTCGCTTCGAAGCACGC
CGAGTGGGACCCCGAGGGCTTCTTCTGCGACGATAGGTGGAAAGCTGTCCAGGCAGAAATCCGTAGCACACATTGCGTTGCAGGGTTTGAGGTTTATTAGTAAAACGGCGGAAGATACGAACGCGGATTTTCAAGTGTTTTGGAAAGCCGTCGATGCGCGTTTCGATCAGCTGGCTTCTTCGGATGGCTTAAGGCTTTCT
CGTGCTGACTTCGGCCTTTGCATTGGTATGATGGATTCAAATGAATTTGCACTGGAGCTCTTTGATGCTCTGGCTCGAAGACGAGGATGGCATGATGTAGAGTTCGTTAACAAGGAGCAGCTTCGCGATTTCTGCGTGCAGATCTCCGCTCAAAGCTTTGATTCTCGTCTGCAAATTTTCTTTGACATGTGAGATAATTC
GGCTATTTTTAGCAGCGCGGATGTAGATTCAAGATTTAAGTATCTTTCCCTTCAGACGCTAAAATTTGGACGACTCGTGCTGGCCTTGTAATTTTATCGGGAATTTTGCTATTAAGTCTTTGACATTGTCACTTAGCTTTCATCTTTAAATGTTCTGAGCGAGAGTTCCTTTTACTGTATCTGGTTTGCTTGTTGCTTAA
GCTTTTTCAGAGTACTGAACTCGTACATTTTGTTTTGATTTAAATTGTGCAGGTGTGATAAGAATGCAGACGGACGGATTTCGGAGGACGAAGTAAAAGAGgtaaatcttttcctaaacatgtaatttctttgaaatgcattgaaggtatggatttttatgtaaagcatactgaaaattgctaaataaatcagtaattta
atcgaatattatatcctagGTTATTATGCTCAGTGCATCAGCTAACAAGCTTACGAAGCTCATGGAACAAGCTGAAGAGTACGCAAATTTAATCATGGAAGAGCTGGATCCAGATCATTTGGGTTACATTGAGGTCAACTTTGCTATCTGCAAATCAAATTTTCAAGCAAATTTATCTATTATTATTATATTTTTATTTA
TAGATGACCTCGTATCGCTAATTTCTGCTCAATTTGATCAAGAAACTGAAAACCTTGCTTATAATGCTTTGGTGC

Intron 13 GTATGTTAAA ... CCCTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.38	11	0.7	10.7
A2	45	11	6.8	9
A3	0	14.2	2.8	0
A4	18.52	16.5	2.6	3.7
A5	54.76	10	7	12
A6	15.6	32.5	3	6
A7	10.44	43	5.3	5
A8	32.65	14.5	0.8	7
A9	16.93	29.5	2.6	6
A10	27.5	29	2.3	11

>TRINITY_DN131744_c2_g1_i13 from sample A1 / Example spliced isoform

TTTGGTGAATTTGACTAAGGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGA
AAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACAT
GGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCG
AATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACT
TGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAGTAAGGACATTTCAAGTTGTTTTCTGTTAATTTCAAACCACATGTCTAACTTCCATTTAAAACGCCAGAAAATTTCTAATTTTGTTATAAAGTGCTTAAGAATTTAGCTTAAACGAAAATAAACAAACACGATTTGCTCTAGGAATTTGAT
GATGTTTATCCACCGAGAAAGTTCAGGGCTTGAAATGATTTGGATGTGTAGACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGG
GTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGGTGATAACTGCTTTAGTATTTTAAGCTTCAGTAC
TATGAAGTTTCCTGTAGGCTGTAAATTCTAATCACAGATCTTTATAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCCTTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAAC
GTGTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGC
AGAGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTT
CTTCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCT
GTTGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT

>TRINITY_DN131744_c2_g1_i11 from sample A1 / Example unspliced isoform:

TTTGGTGAATTTGACTAAGGTTTTAAATTTCATATTACCTTTCAGCTAAGGCAACTGAAAGCTCTCCTGCAAGGCTCGACTTATGCAAAGGAAGGCCATACAAACTATAGTCAAAATTTGAGCCAGCAGATATTACCGGTTCTGATCAGGAAGAATCCCATACAGAGAACGATGCAGAGCGCGAGATATTTAATGGACGA
AAATTGGCAAAGGGCCTGGGTTATTGCCATATGGCTGACGACAATGTTGGGGCTATTCACGTGGAAATTCATAGAGTACAGGAACAGAGACATCTTCAAAGTAGCGGGATATTGCCTCTGTGTGGCCAAAGGAAGCGCGGAAACCCTCAAACTTAACATGGCACTCATATTGTTCCCCGTCTGCAGGAACACGATAACAT
GGCTTCGCTCCACGATCTTAGGATCCATTGTGCCATTTGACGACAACATCAATTTCCACAAGACCATCGCCGGAGCCATTGCTGTCGGCGTCATGCTTCATGGCCTCGATCATATAGGCTGCGATTTCATTAGAATTGCAAATTGCGACGAGGAATCGTATAATCTTTATCTTAGAGAATATTTTGGCAGTGAAAAGCCG
AATTACATAGATATCTTAGGAAGCACAGTAGGCATTACGGGGGTTTTTATGGTCGTCTTCATGGCTATTGCCTTCGTACTCGCGACCTCCTGGTTTAGAAGAAGTTCAGTGAAACTTCCTTGGCAACTTGATAAACTAACCGGATTCAACGCTTTCTGGTATTCGCATCACGCATTTGTCGTGATTTACGTGCTTCTACT
TGTTCATTCCATTCACCTTTTCTTCGTGCGAGATTGGAGGGAAAAATCAGTAAGGACATTTCAAGTTGTTTTCTGTTAATTTCAAACCACATGTCTAACTTCCATTTAAAACGCCAGAAAATTTCTAATTTTGTTATAAAGTGCTTAAGAATTTAGCTTAAACGAAAATAAACAAACACGATTTGCTCTAGGAATTTGAT
GATGTTTATCCACCGAGAAAGTTCAGGGCTTGAAATGATTTGGATGTGTAGACTTGGATGTATATTGCCGTTCCGGTGCTACTATATTGCGGCGAGCGCACACTCAGAGCATTCAGAGCAGGGAATTATACTGTCAATATTGTCAAGGCCGCAGTTTATCGCGGAAACATCTTGGCTTTGCATATGTCAAAACCTGCAGG
GTTCGAGTACAAGAGCGGAATGTATTTGTTTCTCCAGTGTCCCGAGATCTCGCCGTTCGAATGGCATCCATTCTCGATCACCTCTGCTCCGGATGATGAACACATTAGCGTGCATATCAGAACAGTTGGAGACTGGACACAGGAGATGAAGAGAGTTTTCTCTCAGACAATGGAGCCCCCTACAGCTGGAAAGAGCGGCC
TTCTAAGAGCCGAGCGTGCAACTGGACTCTACGGAGACGACCAAAGCAAATCCAAgtatgttaaagataactgaccaaatcaaaacctcagatatccaagattcaaagtatttaacaaaacgaaccctgtgcagGTTTCCCAAGCTTCGGATCGACGGACCATACGGAGCTCCGGCACAAAATTACAAAAAATATAACGT
GTTACTCCTGATCGGGCTCGGAATCGGCGCTACTCCTTTCATAAGCATTCTCAAGGACATGTTAAATCATATGAAAATCGCAGATTCTGCTTCGAAGAGGAAAAATAAAGACAGGGGCCCGACGAACGCTTATTTCTACTGGGTCACGAGGGAGCAATGCTCCTTCGACTGGTTCAAGGGAATCATGAACGAAATAGCAG
AGATGGACCGCAAGGCCACCATAGAAATGCACAACTACCTTACTAGCGTTTATGAAGAAGGCGATGCTAGGTCGGCTTTAATTACAATGGTGCAAGCTCTGAATCATGCCAAGAATGGCGTCGATATTCTGTCAGGAACCAGGGTCAAAACTCACTTTGCCAGGCCAAACTGGAAAAAGGTTTTTTCAAAAGTTGCTTCT
TCTAATAGAGATGCTAGAATTGGGGTGTTCTATTGCGGGCCTCTAATTTTGGCCAAGGAGCTGAGAGCCCTAGCCAAAGATTACAACCACAGATTGAACACTAAATTCGAATTCCACAAAGAAAATTTCTAACACAGGCAAGCGGTTTACATTATTTCAACTCTGGATCAGATTTCTACATTATTAACTTAGAGTGCTGT
TGTATTCTAATTTCTGGAAAAGATCAGATTTCTACATTATTAATTTAGAGTGCTGTTGTATCCTAATTTCTGGAAAAAGAAAAGGTAAGATTTAATTTCATAGACTGCCATTTCAT