sp|Q9LJK3|LOH2_ARATH ----> Ceramide synthase LOH2

Intron 1 GTAAGTGGGT ... ACAATTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.29	9	2	1
A2	2.7	18	1	0
A3	7.69	12	1	1
A4	0	4	0	0
A5	27.27	4	0	3
A6	4.6	83	5	3
A7	7.23	77	8	4
A8	11.88	66.8	6	12
A9	9.75	64.8	7	7
A10	4.76	40	2	2

>TRINITY_DN37367_c0_g2_i2 from sample A8 / Example spliced isoform

GGAAACTGTTACAAGGAAAATTGAACAGGGGAATATTACAAAATGTACGGAAACCATGTGGAAGTTGACATATTATATGGGTGTACAAATATTTATTTTTTATATATTATATAAGGAGCCTTGGTTTGGTGAAAGAAAACAATTGTTCGAAGGATGGCCAGATCAAACCATCAAGTTTCCACTGAAACTTTTCTACATGT
GCCAGTGTGGATTTCATATTTACAGTATTCCTGCACTCCACATGCGGCAAACTCGAAGGAAAGACTTTATTGTCATGATGTCGCATCATGTGACTACAGTGTTCTTGACTGGGTATTCATACATTACACGGTTTTTCCGCATTGGTTCTATTGTTTTTGCCTTGCATGATACAAGTGACGTGCTCTTGGAAACAACGAAG
CTCTTTATGTATGGTGGCAAGGATTTTGCAGCTGTCATGAGCTTCGGGGTTTTTTCCCTTTCATGGCTTTTGCTCCGCTTGATATACTACCCATTCTGGATTATCCGGAGTTTAAGTTATGATGGTATTCAGTGCCTAGCTCTCCCTGACCCCTATCACATATGGATCTATTATGTATTTAATACATTGCTTCTGACTCT
CCTTGTGTATCATATATATTGGTGGAGACTCATATGTTCCATGGTTCTGAAGCAGTTCAAAAATAAAGCGAAAGCTTTCGAAGACATTAGATCAGATTCTGAAGACGAGTAGCAGCTGAAGCACGGATATTCTGGGCATTGTTGGAGAGGTATTGTGTTTTCAGGCTTGTATAAGGATATCCCAAAGACCATTGTGTTCT
AGTTTAGGGGTTGATAGCTGATTCAATTGTATACTATGAACACCACTTAGATCGGTGTCTATTGAAAATACGCTTTATGTTTTAATTTGGCCAAGCTCATCTTTTCAATTCTTTTAAAATCAAATGTAATTGAAGATATATCCAATAGCCCATACTGTAGCTTAATGTCCACCATCTTTGAACTCATCAACCTGTTTATT
GAACCTAACTTCATATAAATAATATAACAGTGAAAGAATGAAC

>TRINITY_DN37367_c0_g2_i7 from sample A8 / Example unspliced isoform:

GGAAACTGTTACAAGGAAAATTGAACAGGGGAATATTACAAAATGTACGGAAACCATGTGGAAGTTGACATATTATATGGGTGTACAAATATTTATTTTTTATATATTATATAAGGAGCCTTGGTTTGGTGAAAGAAAACAATTGTTCGAAGGATGGCCAGATCAAACCATCAAGTTTCCACTGAAACTTTTCTACATGT
GCCAGTGTGGATTTCATATTTACAGTATTCCTGCACTCCACATGCGGCAAACTCGAAGGAAAGACTTTATTGTCATGATGTCGCATCATGTGACTACAGTGTTCTTGACTGGGTATTCATACATTACACGGTTTTTCCGCATTGGTTCTATTGTTTTTGCCTTGCATGATACAAGTGACGTGCTCTTGGAAACAACGAAG
CTCTTTATGTATGGTGGCAAGGATTTTGCAGCTGTCATGAGCTTCGGGGTTTTTTCCCTTTCATGGCTTTTGCTCCGCTTGATATACTACCCATTCTGGATTATCCGGAGTTTAAGTTATGATGGTATTCAGTGCCTAGCTCTCCCTGACCCCTATCACATATGGATCTATTATGTATTTAATACATTGCTTCTGACTCT
CCTTGTGTATCATATATATTGGTGGAGACTCATATGTTCCATGGTTCTGAAGCAGTTCAAAAATAAAGCGAAAGCTTTCGAAGACATTAGATCAGgtaagtgggtagattccattttatccacaatcaagtgaatgccatgaatgatctttttacttgtcacatattcttgttttccacaagtttctgttcttaattaaa
tgtctcaagggaagagatctgcatgtttcccgttgcaactaaattctatctcattaccaatgagccatgcatctcatagagtattttgaccttcacaattctagATTCTGAAGACGAGTAGCAGCTGAAGCACGGATATTCTGGGCATTGTTGGAGAGGTATTGTGTTTTCAGGCTTGTATAAGGATATCCCAAAGACCA
TTGTGTTCTAGTTTAGGGGTTGATAGCTGATTCAATTGTATACTATGAACACCACTTAGATCGGTGTCTATTGAAAATACGCTTTATGTTTTAATTTGGCCAAGCTCATCTTTTCAATTCTTTTAAAATCAAATGTAATTGAAGATATATCCAATAGCCCATACTGTAGCTTAATGTCCACCATCTTTGAACTCATCAAC
CTGTTTATTGAACCTAACTTCATATAAATAATATAACAGTGAAAGAATGAAC

Intron 2 GTTTTTCCGC ... ATGATACAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	51.72	7	10	5
A2	66.67	2	6	2
A3	75	2	10	2
A4	71.43	2	6	4
A5	100	0	3	1
A6	64.38	13	29	18
A7	63.89	13	36	10
A8	57.14	12	17	15
A9	51.22	10	16	5
A10	60	7	15	6

>TRINITY_DN37367_c0_g2_i6 from sample A8 / Example spliced isoform

GGAAACTGTTACAAGGAAAATTGAACAGGGGAATATTACAAAATGTACGGAAACCATGTGGAAGTTGACATATTATATGGGTGTACAAATATTTATTTTTTATATATTATATAAGGAGCCTTGGTTTGGTGAAAGAAAACAATTGTTCGAAGGATGGCCAGATCAAACCATCAAGTTTCCACTGAAACTTTTCTACATGT
GCCAGTGTGGATTTCATATTTACAGTATTCCTGCACTCCACATGCGGCAAACTCGAAGGAAAGACTTTATTGTCATGATGTCGCATCATGTGACTACAGTGTTCTTGACTGGGTATTCATACATTACACGTGACGTGCTCTTGGAAACAACGAAGCTCTTTATGTATGGTGGCAAGGATTTTGCAGCTGTCATGAGCTTC
GGGGTTTTTTCCCTTTCATGGCTTTTGCTCCGCTTGATATACTACCCATTCTGGATTATCCGGAGTTTAAGTTATGATGGTATTCAGTGCCTAGCTCTCCCTGACCCCTATCACATATGGATCTATTATGTATTTAATACATTGCTTCTGACTCTCCTTGTGTATCATATATATTGGTGGAGACTCATATGTTCCATGGT
TCTGAAGCAGTTCAAAAATAAAGCGAAAGCTTTCGAAGACATTAGATCAGATTCTGAAGACGAGTAGCAGCTGAAGCACGGATATTCTGGGCATTGTTGGAGAGGTATTGTGTTTTCAGGCTTGTATAAGGATATCCCAAAGACCATTGTGTTCTAGTTTAGGGGTTGATAGCTGATTCAATTGTATACTATGAACACCA
CTTAGATCGGTGTCTATTGAAAATACGCTTTATGTTTTAATTTGGCCAAGCTCATCTTTTCAATTCTTTTAAAATCAAATGTAATTGAAGATATATCCAATAGCCCATACTGTAGCTTAATGTCCACCATCTTTGAACTCATCAACCTGTTTATTGAACCTAACTTCATATAAATAATATAACAGTGAAAGAATGAAC

>TRINITY_DN37367_c0_g2_i2 from sample A8 / Example unspliced isoform:

GGAAACTGTTACAAGGAAAATTGAACAGGGGAATATTACAAAATGTACGGAAACCATGTGGAAGTTGACATATTATATGGGTGTACAAATATTTATTTTTTATATATTATATAAGGAGCCTTGGTTTGGTGAAAGAAAACAATTGTTCGAAGGATGGCCAGATCAAACCATCAAGTTTCCACTGAAACTTTTCTACATGT
GCCAGTGTGGATTTCATATTTACAGTATTCCTGCACTCCACATGCGGCAAACTCGAAGGAAAGACTTTATTGTCATGATGTCGCATCATGTGACTACAGTGTTCTTGACTGGGTATTCATACATTACACGgtttttccgcattggttctattgtttttgccttgcatgatacaagTGACGTGCTCTTGGAAACAACGAAG
CTCTTTATGTATGGTGGCAAGGATTTTGCAGCTGTCATGAGCTTCGGGGTTTTTTCCCTTTCATGGCTTTTGCTCCGCTTGATATACTACCCATTCTGGATTATCCGGAGTTTAAGTTATGATGGTATTCAGTGCCTAGCTCTCCCTGACCCCTATCACATATGGATCTATTATGTATTTAATACATTGCTTCTGACTCT
CCTTGTGTATCATATATATTGGTGGAGACTCATATGTTCCATGGTTCTGAAGCAGTTCAAAAATAAAGCGAAAGCTTTCGAAGACATTAGATCAGATTCTGAAGACGAGTAGCAGCTGAAGCACGGATATTCTGGGCATTGTTGGAGAGGTATTGTGTTTTCAGGCTTGTATAAGGATATCCCAAAGACCATTGTGTTCT
AGTTTAGGGGTTGATAGCTGATTCAATTGTATACTATGAACACCACTTAGATCGGTGTCTATTGAAAATACGCTTTATGTTTTAATTTGGCCAAGCTCATCTTTTCAATTCTTTTAAAATCAAATGTAATTGAAGATATATCCAATAGCCCATACTGTAGCTTAATGTCCACCATCTTTGAACTCATCAACCTGTTTATT
GAACCTAACTTCATATAAATAATATAACAGTGAAAGAATGAAC

Intron 3 GTTTTTATAT ... TTTCCTGCAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.13	15.3	6	3.7
A2	0	11	0	2
A3	8.73	15	1	0
A4	0	7.7	0	0.7
A5	0	2.7	0	0
A6	15.45	83.3	4	8.7
A7	24.17	58	9	8
A8	15.78	57	5	6
A9	20.54	55.7	5	2
A10	17.41	42	3	2.3

>TRINITY_DN37953_c1_g2_i3 from sample A6 / Example spliced isoform

TAAGTCGCATTTACCGCATCATTATGATAGAGCCGGATATGTAATTCTTCACAGTAAGGTTCAGGTCTTTTCGGCGACCGGAATCTATCAGCCATGCCGTTTTAACTAGGGGTTTCAAATCGAAAATGCAATTTCTTTTCATGGTAATTCACTGAGGCAGAGCTTCTTCTGGTGGTCTTCAATGGAGGCGATTTGGAGAC
GGGAAAGTTTGCCAGAGGCGAAGGACTTCTATCTAGCGATCTGCGCTGCCTTTGCGTGTTTTTCCCTCCATTTTCTTCTGAACGTCGCTTTGTACCAGGTTTCCACTGAAACTTTTCTACATGTGCCAGTGTGGATTTCATATTTACAGTATTCCTGCACTCCACATGCGGCAAACTCGAAGGAAAGACTTTATTGTCAT
GATGTCGCATCATGTGACTACAGTGTTCTTGACTGGGTATTCATACATTACACGGTTTTTCCGCATTGGTTCTATTGTTTTTGCCTTGCATGATACAAGTGACGTGCTCTTGGAAACAACGAAGCTCTTTATGTATGGTGGCAAGGATTTTGCAGCTGTCATGAGCTTCGGGGTTTTTTCCCTTTCATGGCTTTTGCTCC
GCTTGATATACTACCCATTCTGGATTATCCGGAGTTTAAGTTATGATGGTATTCAGTGCCTAGCTCTCCCTGACCCCTATCACATATGGATCTATTATG

>TRINITY_DN37953_c1_g2_i4 from sample A6 / Example unspliced isoform:

TAAGTCGCATTTACCGCATCATTATGATAGAGCCGGATATGTAATTCTTCACAGTAAGGTTCAGGTCTTTTCGGCGACCGGAATCTATCAGCCATGCCGTTTTAACTAGGGGTTTCAAATCGAAAATGCAATTTCTTTTCATGGTAATTCACTGAGGCAGAGCTTCTTCTGGTGGTCTTCAATGGAGGCGATTTGGAGAC
GGGAAAGTTTGCCAGAGGCGAAGGACTTCTATCTAGCGATCTGCGCTGCCTTTGCGTGTTTTTCCCTCCATTTTCTTCTGAACGTCGCTTTGTACCAGGTTTCCACTGAAACTTTTCTACATGTGCCAGTGTGGATTTCATATTTACAGTATTCCTGCACTCCACATGCGGCAAACTCGAAGGAAAGACTTTATTGTCAT
GATGTCGCATCATGTGACTACAGTGTTCTTGACTGGGTATTCATACATTACACGGAAAGCTTCTTTGTAAAGAGTGAAGTATTGATTTTATGATCAAGTAGTTTCTCCACTTTTGACTTTGCACTCGATCAAATGAAAAGCCAGAAAAAGGTCAATGAGATGAACCTTAATTGTTAATTGAAAGTTTGGATGAAATATGG
AACTTAATCTCCAAATGATCATTTTTTTTGTTGATGTCGAAGTACTCACGAGATTATTCCTGGATAGAAGTTTTAGGCACTCTTCTTCATGTATAACCAGGTTTTTCCGCATTGGTTCTATTGTTTTTGCCTTGCATGATACAAGTGACGTGCTCTTGGAAACAACGAAGCTCTTTATGTATGGTGGCAAGGATTTTGCA
GCTGTCATGAGCTTCGGGGTTTTTTCCCTTTCATGGCTTTTGCTCCGCTTGATATACTACCCATTCTGGATTATCCGGAGTTTAAGgtttttatatcctttctcagatcttcaattcatgagatgtcggtcaaatctctgaagcttctttgaattataaaatgatacttacagtatgggttttgcacttttttcctgcag


TTATGATGGTATTCAGTGCCTAGCTCTCCCTGACCCCTATCACATATGGATCTATTATG