NA ----> NA

Intron 1 GTATTATTTT ... ACTTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	73.41	28	50	106
A2	75.02	25	63	88.5
A3	77	22	39	108.5
A4	75.64	29	73	108
A5	79.84	10	37	43
A6	63.13	71	74	171
A7	65.85	66	82	175
A8	61.6	61	65	135
A9	66.41	62	89	159.5
A10	69.8	52	88	154

>TRINITY_DN114639_c1_g1_i6 from sample A2 / Example spliced isoform

CTAAAATTTGGTTAAATCTGGAGAAAGAAGTCTCTATCGAAAAGCTTTTGCTTTCTTGCACAAAAGCATTTCAACGGTTCGAACTCTTGTCTGTATGCTGCATTTTTTTTTCAACTGTTCAGAAGCTTTTTGTCTCTAGCCGTTGGATGCTTGATCTCTATTTAAATCCACTTCAACTCGAATCCGGGCACAATTTCAGA
AATAGCGTTCCAACTAGCTTTTGTGTTATTTGCTATTTTGCTCTGTCTCTGTCTCTGGCTCTGGCTAATTGAAGATCCACAATCCGGCATGGAGTTCGAGCGATTCCTGATGAGGCCAGGCAAAAAAACCGTTTCTAAATAATATTCTTCTTCTTAAAAGCTGCGTTCATATGATTTCCATGCTTTTATTAGAAATAAGG
GTTTATGTAATTAAAGGGCTTCAAATTTGTTTTGAGACTCGGATACATCACAATTCCTTATATCCTAGTACTATAATCTATTCAAAATGTGCTGTATCTGGCAA

>TRINITY_DN114639_c1_g1_i1 from sample A2 / Example unspliced isoform:

CAACTGTTCAGAAGCTTTTTGTCTCTAGCCGTTGGATGCTTGATCTCTATTTAAATCCACTTCAACTCGAATCCGGGCACAATTTCAGAAATGGCGTCCCAACTAGCTTTTGTGTgtattattttgctctggcctttcacttttttttttagttatggcctttctgtattttcctttttattggagtcttgtttgtgttt
ctatgatgcattgctgttttccttcttttccattggaatttttgttgctgagttttgagttttgtttgcgtggctgccttcactgctgtgtttttttgtctggttttatatgacgcaagagactaaaacctgtatacggatggacttcttcagTATTTGCTATTTTGCTCTGTCTCTGTCTCTGGCTCTGGCTAATTGAA
GATCCACAATCCGGCATGGAGTTCGAGCGATTCCTGATGAGGCCAGGCAAAAAAACCGTTTCTAAATAATATTCTTCTTCTTAAAAGCTGCGTTCATATGATTTCCATGCTTTTATTAGAAATAAGGGTTTATGTAATTAAAGGGCTTCAAATTTGTTTTGAGACTCGGATACATCACAATTCCTTATATCCTAGTACTA
TAATCTATTCAAAATGTGCTGTATCTGGCAA

Intron 2 GTGTGTATTA ... ACTTCTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.76	41	51.7	120.7
A2	72.91	28	62.7	88
A3	68.8	35	53.3	101
A4	72.73	34	63.3	118
A5	82.25	10	41.7	51
A6	54.9	109	93.3	172
A7	60.17	92	101	177
A8	55.71	77	75.7	118
A9	59.12	90	115.3	145
A10	64.73	72	104.3	160

>TRINITY_DN127071_c0_g1_i12 from sample A1 / Example spliced isoform

TAAAATTTGGATAAGTCTGGAGAAAGAAGTCTCCATCGAAAAGCTTTTGCTTTCTTGCACAAAAGCATTTCAACGGTTCGAACTCTTGTCTGTATGCTGCATTTTTTTTTTCAACTGTTCAGAAGCTTTTTGTCTCCAGCCGTTGGATGCTTGGTCTCTATTTAAATCCACTTCAACTCGAATCCGGGCACAATTTCAGA
AATGGCGTCCCAACTAGCTTTTTATTTGCTATTTTTCTCTGGCTCTGGCTAATTGAAGATCCACAATCCGGAATGGAGTTCGAGCGATTCCTGAGGAGACCAGGACTGTAATCAAATCAAAATGTGCTGTATCTGGCAATTGCAGAGGATTGGTTTTGATGCTGCTCGAGAGGATTGGTATTTAAATACAGTCATGAAAT
AATAAATCTGTGAGTCATTTTACCAGCTCGGCACATATATTTCCTATGCAGATATGAACGATGTTCTATGCAGCACACACATAGAAATAGCTTTTCTGTTTTTTTGAGATTTTGCATTTTTAATTAGCTTTACAGTTTTTTATGCATCATAGTTCTTTGGTTCTTGTTTTAGATTTAATCGTAAGTAATTACAATTTTAA
ACCCTAAATTATTCATGTCATAGAATAAAATCAATTTTGTTAAGAGGAAACTTAATCTGTTCTTATTTTTATATTGACGGCAACTAGATCTGGTTTTAGAGATATCTGTCGATAATGTATGGCTTCTAAAAGTGTATGGATTCCAGTAGAGATTATGATAGTAACAACAACGAAATACAGTAGTTCTCTGGTTCTTGTTT
TAGAGTTAGTTGTAATTATACAAAACGTAGGGCGTTATACAAAACGTAGGGCAGCATTTATTTTTCTTTTAAAAGGTCTCTGAATCCCCGTGAAGTGAGGTACAAGCCAAAAGCCCAAATCGTAGGATCATACTTGCAAAAGTGGAAAGACAGAATCATACTCGCAAAATAAAAATAGAGAGACAGAATCATATTCGCAA
AATAAAAATAGAGAGACAGAATCATACTCGCAAAATCAAAATAGAATAAGAATAGAGAGACAGAATCATACTCGCAAAATCAAAATAGAATAAAAATAGAGAGACAGAATCATACTCGCAAAATCAAAATAGAATAATTCGCAAAATAAAAATAGAGAGAC

>TRINITY_DN127071_c0_g1_i8 from sample A1 / Example unspliced isoform:

TAAAATTTGGATAAGTCTGGAGAAAGAAGTCTCCATCGAAAAGCTTTTGCTTTCTTGCACAAAAGCATTTCAACGGTTCGAACTCTTGTCTGTATGCTGCATTTTTTTTTTCAACTGTTCAGAAGCTTTTTGTCTCCAGCCGTTGGATGCTTGGTCTCTATTTAAATCCACTTCAACTCGAATCCGGGCACAATTTCAGA
AATGGCGTCCCAACTAGCTTTTgtgtgtattattttgctatggcctttcccttttttttttcttctagttatggcctttctttattttcctttttattggagtcttgtttgtgtttctatgatgcattgctgttttccttcttttcccttggaatttttgtttctgagttttgagttttgtttgcgtggctgccttcaat
gctgtgctgtgttttttttgtctggttttgtatgacgcaagagactaaaacctgtatacgtatggacttcttcagTATTTGCTATTTTTCTCTGGCTCTGGCTAATTGAAGATCCACAATCCGGAATGGAGTTCGAGCGATTCCTGAGGAGACCAGGACTGTAATCAAATCAAAATGTGCTGTATCTGGCAATTGCAGAG
GATTGGTTTTGATGCTGCTCGAGAGGATTGGTATTTAAATACAGTCATGAAATAATAAATCTGTGAGTCATTTTACCAGCTCGGCACATATATTTCCTATGCAGATATGAACGATGTTCTATGCAGCACACACATAGAAATAGCTTTTCTGTTTTTTTGAGATTTTGCATTTTTAATTAGCTTTACAGTTTTTTATGCAT
CATAGTTCTTTGGTTCTTGTTTTAGATTTAATCGTAAGTAATTACAATTTTAAACCCTAAATTATTCATGTCATAGAATAAAATCAATTTTGTTAAGAGGAAACTTAATCTGTTCTTATTTTTATATTGACGGCAACTAGATCTGGTTTTAGAGATATCTGTCGATAATGTATGGCTTCTAAAAGTGTATGGATTCCAGT
AGAGATTATGATAGTAACAACAACGAAATACAGTAGTTCTCTGGTTCTTGTTTTAGAGTTAGTTGTAATTATACAAAACGTAGGGCGTTATACAAAACGTAGGGCAGCATTTATTTTTCTTTTAAAAGGTCTCTGAATCCCCGTGAAGTGAGGTACAAGCCAAAAGCCCAAATCGTAGGATCATACTTGCAAAAGTGGAA
AGACAGAATCATACTCGCAAAATAAAAATAGAGAGACAGAATCATATTCGCAAAATAAAAATAGAGAGACAGAATCATACTCGCAAAATCAAAATAGAATAAGAATAGAGAGACAGAATCATACTCGCAAAATCAAAATAGAATAAAAATAGAGAGACAGAATCATACTCGCAAAATCAAAATAGAATAATTCGCAAAAT
AAAAATAGAGAGAC

Intron 3 GCAAAAAAAC ... GCAATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	36.22	26	9.2	6.1
A2	45.25	27.3	18	9.3
A3	52.81	27.1	28.8	9.7
A4	41.1	21.8	15.3	2.7
A5	42.58	15.8	11.2	3
A6	24.32	67.1	12.2	20.6
A7	26.86	68.7	18	24.3
A8	28.98	71.3	26.8	16.3
A9	29.38	73.2	17.3	32.7
A10	39.01	45.2	17.6	25.3

>TRINITY_DN44390_c2_g1_i4 from sample A6 / Example spliced isoform

TAAAAACAGGCGGACAACACATGCAGATTCAGCTATGTTAATGACAAATGTAAGCAGAACGCTGCCAGCTTCTAACTAAAATTTGGATAAGTCTGGAGAAAGAAGTCTCTATCGAAAAGCTTTTGCTTTCTTGCACAAAAGCATTTCAACGGTTCGAACTCTTGTCTGTATGCTGCATTTTTTTTTCAACTGTTCATAAG
CTTTTTGTCTCTAGCCGTTGGATGCTTGGTCTCTATTTAAACCCACTTTAACTCGAATTCGGGCACAATTTCAGAATGGCGTTCCAACTAGCTTTTGTGTGTAATATTTTGCTTTGGCCCTTCCCTTTTCTTTTCTAGTTATGACCTTTCTGTATTTTCGTTTTTATTGGAGTCTTGTTTGTGTTGCTATGATGCATTGC
TGTTTTCCTTCTTTTCCCTTGGAATTTTTGTTGCTGAGTTTTGAGTTTTGTTTGCGTGGCTGCCTTCACTGCTGTGTTTTTTTGTCTGGTTTTATATGACGCAAGAGACTAAAACCTGTATACGGATGGACTTCTTCAGTATTTGCTATTTTGCTCTGTCTCTGTCTCTGGCTCTGGCTAATTGAAGATCCACAATCCGG
CATGGAGTTCGAGCGATTCCTGATGAGGCCAGAGGATTGGTTTTGATGCTGCTCGAGAGGATTGGTATTTAAATACAGTCATGAAATAATAAATCTGTACAAGCCAAAAGCCCAAATCGTGTGAAGTGGAAAGTGGAAAGACAGAATCCTACTCGCAAAGACAGAATCATTCTCGCAAAATAAAAATAAAAATAGTTATA
GAGGCAGAGCATAGTTATATAGTTATAGAGGCAGAGCATAGTTATATAGTTATAGAGGCAGAGCATAG

>TRINITY_DN44390_c2_g1_i15 from sample A6 / Example unspliced isoform:

TAAAAACAGGCGGACAACACATGCAGATTCAGCTATGTTAATGACAAATGTAAGCAGAACGCTGCCAGCTTCTAACTAAAATTTGGATAAGTCTGGAGAAAGAAGTCTCTATCGAAAAGCTTTTGCTTTCTTGCACAAAAGCATTTCAACGGTTCGAACTCTTGTCTGTATGCTGCATTTTTTTTTCAACTGTTCATAAG
CTTTTTGTCTCTAGCCGTTGGATGCTTGGTCTCTATTTAAACCCACTTTAACTCGAATTCGGGCACAATTTCAGAATGGCGTTCCAACTAGCTTTTGTGTGTAATATTTTGCTTTGGCCCTTCCCTTTTCTTTTCTAGTTATGACCTTTCTGTATTTTCGTTTTTATTGGAGTCTTGTTTGTGTTGCTATGATGCATTGC
TGTTTTCCTTCTTTTCCCTTGGAATTTTTGTTGCTGAGTTTTGAGTTTTGTTTGCGTGGCTGCCTTCACTGCTGTGTTTTTTTGTCTGGTTTTATATGACGCAAGAGACTAAAACCTGTATACGGATGGACTTCTTCAGTATTTGCTATTTTGCTCTGTCTCTGTCTCTGGCTCTGGCTAATTGAAGATCCACAATCCGG
CATGGAGTTCGAGCGATTCCTGATGAGGCCAGgcaaaaaaaccgtttctaaataatattcttcttcttaaaagctgcgttcatgtgatttccatgcttttattagaaataagggtttatgtaattaaagggcttcaaatttgttttgagactcggatacatcacaattccttatatcctagggctataatctaatcaaaa
tgtgctgtatctggcaattgcagAGGATTGGTTTTGATGCTGCTCGAGAGGATTGGTATTTAAATACAGTCATGAAATAATAAATCTGACTGATCTCTTGATATGCGGTGTTTGCGTTCGGGTTTTTCCATGCAGCGCTAATATTTATGCAGATCTGAAGCTTTGAGTGGTGTTTCAGTCTTTCAATTTTAGTTGTAGGA
CAAATTCTTTGAGTCCAAATTTGTGTAACATGGAGATGTAAAATTACAACACAAGACATATTGCTTTTATCTTTGAGATGAAGTGCTAGATAAGTTTTGAAGGGTTTTAACTCCCGCAATTGTAGGAAGGAATTTTCTCCTTTAAAGGTGATTTTGCATCCCTTTTCCAAAGGAAAGCAATAACAGACACTCACATATTA
TGCCAA