sp|Q9ZT66|E134_MAIZE ----> Endo-1,3;1,4-beta-D-glucanase

Intron 1 GTAAGCAATA ... TGATGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.55	114.9	8.9	58
A2	32.71	205.8	11.8	100
A3	36.63	224.9	24.9	130
A4	33.7	122	10	62
A5	35.82	86	12	48
A6	36.97	1397.9	120.8	820
A7	35.78	1339	86.9	746
A8	33.96	820.8	65.4	422
A9	36.31	1439.9	130.9	821
A10	38.86	1135.8	133.9	722

>TRINITY_DN103739_c0_g1_i3 from sample A2 / Example spliced isoform

CTAAAAAACACTACAAAAACATATCAAACTACATGACAAAGAACTTGGAGTATTTATAGCCCAGACAAGGGCATTCAATCTGAACCGAAGGCAGCTTGGTCTGGGAAAAATACAAGAACAATTCATTGTGGTGGAGAGCCATGGTTTCTATTCTCGCGCAGGAGCGTATTTTAGGAGCAGCCATGGGAGCTGCATTCGGA
ACAGGCGTGGTCTTGTACCAGCAGACTCTCATTTACAGATCCACTGCTCAGTCAATTGAGTTTATCTCTGGAAACCCACCAGTTACCCGTCCTCCTAAGGTTCCAGAGCCACTATTTAATAAAAAGACTCGTTCAGAGCTTGCTCATGCTTGGAATGTCGGCATTGACAAAAGTTTGGGTGCATTGATTGCATCTCTCAG
CTCTCGAGGATGGGAACTCCAAATCAAGTAACTTGAAGGGTTGGACAGTGGACAAACCAAACTATATTTGGTCTAAATTTCAGCTGCAAAGGCTTGGGAATTTTGAAGGTGCGTGTATACTTTGTGTTTTTGTTCAACTCGAGACATGGTTATAGTAAGGTTTGCTAATAAGTGCAAGGTCCAATTGGATTGACATTGAT
CGAAGTACAAGGTGTTTGAGTCCAACGATGCACGTGGTGAGAATTTGAAAATACCTTATTAAATAGATTGGCAAAAGAAATAAAC

>TRINITY_DN103739_c0_g1_i4 from sample A2 / Example unspliced isoform:

CTAAAAAACACTACAAAAACATATCAAACTACATGACAAAGAACTTGGAGTATTTATAGCCCAGACAAGGGCATTCAATCTGAACCGAAGGCAGCTTGGTCTGGGAAAAATACAAGAACAATTCATTGTGGTGGAGAGCCATGGTTTCTATTCTCGCGCAGgtaagcaataaatgtaacaattctcgaatgcaattggag
agtcatcttcatataattgtgtaatggctggcgttcaaacaatgggcttgatgagcagGAGCGTATTTTAGGAGCAGCCATGGGAGCTGCATTCGGAACAGGCGTGGTCTTGTACCAGCAGACTCTCATTTACAGATCCACTGCTCAGTCAATTGAGTTTATCTCTGGAAACCCACCAGTTACCCGTCCTCCTAAGGTTC
CAGAGCCACTATTTAATAAAAAGACTCGTTCAGAGCTTGCTCATGCTTGGAATGTCGGCATTGACAAAAGTTTGGGTGCATTGATTGCATCTCTCAGCTCTCGAGGATGGTAGTGACAATTTCTTTTCTGAGAATATTTCAGAAAATATGATTTCATGGCTTTTTATTTATGTAAAAGCTGTCAGTATCATGGAAAGCAT
CATCGAAGCCAATACTTTAGAATGTTGTCTTAATAACTTGGCCCACTCTCCATATTTAGTCTTTACATGTTTCAGCATTTTTTGAACTTGTCACTCCTGCAGATAGAGAGCATTACCACAAGATTAATTTGTTATCTAAGGTCATTCCAAATCGTGTTGTCAGAACAATGATTTATGCCTTATGGCAGCTTTTCTCTAGC
AGATATGCTGGGCAGAATTAATCAAATAATTTTTGTTGTGATGGTTTTAATCAAATCCAAAAGATGACTTTGAGTAGATCGGTAGCTACATCTTAGGAAAAATAGTTATTTGAAAGGTTAACAGATTACCAGTGTTCCATGGGAACACGTCTCCCAGGGTCGGGGGCTT

Intron 2 GTAAGCAATA ... CGTATTTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62	38	9	62
A2	53.81	103	12	120
A3	56.2	113	25	145
A4	59.65	46	10	68
A5	61.18	33	12	52
A6	58.41	626	122	879
A7	56.47	619	87	803
A8	58.2	339	66	472
A9	57.58	630	131	855
A10	57.31	590	134	792

>TRINITY_DN103739_c0_g1_i5 from sample A2 / Example spliced isoform

TTTCTATTCTCGCGCAGGAGCAGCCATGGGAGCTGCATTCGGAACAGGCGTGGTCTTGTACCAGCAGACTCTCATTTACAGATCCACTGCTCAGTCAATTGAGTTTATCTCTGGAAACCCACCAGTTACCCGTCCTCCTAAGGTTCCAGAGCCACTATTTAATAAAAAGACTCGTTCAGAGCTTGCTCATGCTTGGAATG
TCGGCATTGACAAAAGTTTGGGTGCATTGATTGCATCTCTCAGCTCTCGAGGATGGGAACTCCAAATCAAGTAACTTGAAGGGTTGGACAGTGGACAAACCAAACTATATTTGGTCTAAATTTCAGCTGCAAAGGCTTGGGAATTTTGAAGGTGCGTGTATACTTTGTGTTTTTGTTCAACTCGAGACATGGTTATAGTA
AGGTTTGCTAATAAGTGCAAGGTCCAATTGGATTGACATTGATCGAAGTACAAGGTGTTTGAGTCCAACGATGCACGTGGTGAGAATTTGAAAATACCTTATTAAATAGATTGGCAAAAGAAATAAAC

>TRINITY_DN103739_c0_g1_i4 from sample A2 / Example unspliced isoform:

CTAAAAAACACTACAAAAACATATCAAACTACATGACAAAGAACTTGGAGTATTTATAGCCCAGACAAGGGCATTCAATCTGAACCGAAGGCAGCTTGGTCTGGGAAAAATACAAGAACAATTCATTGTGGTGGAGAGCCATGGTTTCTATTCTCGCGCAGgtaagcaataaatgtaacaattctcgaatgcaattggag
agtcatcttcatataattgtgtaatggctggcgttcaaacaatgggcttgatgagcaggagcgtattttagGAGCAGCCATGGGAGCTGCATTCGGAACAGGCGTGGTCTTGTACCAGCAGACTCTCATTTACAGATCCACTGCTCAGTCAATTGAGTTTATCTCTGGAAACCCACCAGTTACCCGTCCTCCTAAGGTTC
CAGAGCCACTATTTAATAAAAAGACTCGTTCAGAGCTTGCTCATGCTTGGAATGTCGGCATTGACAAAAGTTTGGGTGCATTGATTGCATCTCTCAGCTCTCGAGGATGGTAGTGACAATTTCTTTTCTGAGAATATTTCAGAAAATATGATTTCATGGCTTTTTATTTATGTAAAAGCTGTCAGTATCATGGAAAGCAT
CATCGAAGCCAATACTTTAGAATGTTGTCTTAATAACTTGGCCCACTCTCCATATTTAGTCTTTACATGTTTCAGCATTTTTTGAACTTGTCACTCCTGCAGATAGAGAGCATTACCACAAGATTAATTTGTTATCTAAGGTCATTCCAAATCGTGTTGTCAGAACAATGATTTATGCCTTATGGCAGCTTTTCTCTAGC
AGATATGCTGGGCAGAATTAATCAAATAATTTTTGTTGTGATGGTTTTAATCAAATCCAAAAGATGACTTTGAGTAGATCGGTAGCTACATCTTAGGAAAAATAGTTATTTGAAAGGTTAACAGATTACCAGTGTTCCATGGGAACACGTCTCCCAGGGTCGGGGGCTT