sp|Q1G3Q4|RITF1_ARATH ----> Protein RGF1 INDUCIBLE TRANSCRIPTION FACTOR 1

Intron 1 GTGTGGCATT ... AAATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.29	3	1	0
A2	-1	0	0	0
A3	0	9	0	0
A4	33.33	2	1	1
A5	-1	0	0	0
A6	31.25	11	4	6
A7	21.43	11	5	1
A8	30	14	8	4
A9	23.08	5	3	0
A10	26.67	11	3	5

>TRINITY_DN36829_c0_g1_i1 from sample A6 / Example spliced isoform

GGCGACCGACAGAAGGTGATATGCTGGCGTGGACTTCAACGTGGGCACCCGCGTCCCTTCGCTACACCTATTACCGGCCTAGGGTTCCGGCTCACCGAAAAAAAGAAGGCGATGATAGCCTGTATTTGAATTTCCAAACCTAGCTGCCTCAGAGATCTAGAGTCAAACAACTGGACGGCTACCGGGCCCGGCACGCTGCT
CTGTTTGTTGGCGCGGCTTTTTGAGGCGTTGTAGGCTCTCTTGATAATTTGGTATCTTTTACTGCTCTTCCTTGTGCTCCAGCCGCCGCCTCCTGGTTTCGGCGAGGATTATGCAGCTAAGGAAATCCCAATTCCATCAATTGCTCAGGTCTCTACGGTATGATTGTCCTCCTTGCCAATTCTTGAAGATCTGTCAGCAG
CGGATTCAAATTCTTAAACGATATGGTGGGATATGCTCTGTGTCCCTCGCTTCCAAAACCAACATGGTTAGAGACACTGCTGACTGATAAATTCTTTGTTCCCTGTTCGAAGCATATTGAACAAAAGAAAAACGAAAGCAATATTTTTTGCATCGATTGTAGCAAAAGTATCTGTCAACACTGTCTTCCCAAGCATCGGT
CGCATCGGCTTCTTCAGGTGCGAAGATATGTGTACCACGACGTTATCCGGCTGCTAGACTTGCAGAAGCTTCTAGATTGCTCGCTAGTGCAGACTTATATCATTAATAGTGCGAAGGTCGTATTTTTGAACCAGCGGCCCCAGTCCAGGCTCCCCAAAGGACTGGGAAACTCTTGCCAAACTTGTGACAGAAGCCTGCAG
GAATCGTACTGTTATTGCTCGGTTTCGTGCAAGGTAAATGCTGTGTTGAATAAAGAAAAGGATTTATCTACATTACTGTACCATTGCAGCTCACTTCCATTTCCTGATATTTTTTACTACTCTCCGAGAGAAATTTGTTACAAGAATAGAAAGTTTGCAGAAATGGGAGAAGAATTTTCGGCTAGCAGTGTGTTAGAAGA
GACATGTTCAGGCTCCAACGCTAACGATGTGCGTTGGATGTTGACATGTTCGGAGATAGACATGCCTTTAGTAAAGAAAACATGCAGGCGTCTTGCTGCACCCCACTCTATTGCCCGGACATCAACTAGCAGGAGAAAGGGTATTCCCCACAGATCGCCAATATATTAATTAATTATAGTTTATTTTTAATAATTTATTT
TGAATGATGCTTAACACTCCAAGAGATCTGTATTGTGCACACCAGCGGAATTTTTGGGTTGGTCTGTTCTCGATGAGAGTCAGTTTACAAGGTTTTCTGTACTACAGAGTGCGTATATTTAACGTCTATGTTAGGCGCCCAAAGATTATCATTACCCGTAGCATTACCTATTACACTGTTTTATATTCATCGATAGTGTT
TTGATATTGTATATTTTGTTTGTCGTACATTGTGCATATCTTTCTAAATAGTCATCAAAATCAAACTAG

>TRINITY_DN36829_c0_g1_i9 from sample A6 / Example unspliced isoform:

GGTGGGATATGCTCTGTGTCCCTCGCTTCCAAAACCAACATGGTTAGAGACACTGCTGACTGATAAATTCTTTGTTCCCTGTTCGAAGCATATTGAACAAAAGAAAAACGAAAGCAATATTTTTTGCATCGATTGTAGCAAAAGTATCTGTCAACACTGTCTTCCCAAGCATCGGTCGCATCGGCTTCTTCAGGTGCGAA
GATATGTGTACCACGACGTTATCCGGCTGCTAGACTTGCAGAAGCTTCTAGATTGCTCGCTAGTGCAGgtgtggcatttgctgtactgaaatgaagaatcgattatctggataaaaaacaattttgtgtctaattaatggttttatttgttggccggaaatttgcagACTTATATCATTAATAGTGCGAAGGTCGTATTT
TTGAACCAGCGGCCCCAGTCCAGGCTCCCCAAAGGACTGGGAAACTCTTGCCAAACTTGTGACAGAAGCCTGCAGGAATCGTACTGTTATTGCTCGGTTTCGTGCAAGGTAAATGCTGTGTTGAATAAAGAAAAGGATTTATCTACATTACTGTACCATTGCAGCTCACTTCCATTTCCTGATATTTTTTACTACTCTCC
GAGAGAAATTTGTTACAAGAATAGAAAGTTTGCAGAAATGGGAGAAGAATTTTCGGCTAGCAGTGTGTTAGAAGAGACATGTTCAGGCTCCAACGCTAACGATGTGCGTTGGATGTTGACATGTTCGGAGATAGACATGCCTTTAGTAAAGAAAACATGCAGGCGTCTTGCTGCACCCCACTCTATTGCCCGGACATCAA
CTAGCAGGAGAAAGGGTATTCCCCACAGATCGCCAATATATTAATTAATTATAGTTTATTTTTAATAATTTATTTTGAATGATGCTTAACACTCCAAGAGATCTGTATTGTGCACACCAGCGGAATTTTTGGGTTGGTCTGTTCTCGATGAGAGTCAGTTTACAAGGTTTTCTGTACTACAGAGTGCGTATATTTAACGT
CTATGTTAGGCGCCCAAAGATTATCATTACCCGTAGCATTACCTATTACACTGTTTTATATTCATCGATAGTGTTTTGATATTGTATATTTTGTTTGTCGTACATTGTGCATATCTTTCTAAATAGTCATCAAAATCAAACTAG

Intron 2 GTATTCCCCA ... TGCACACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.29	3	1	0
A2	100	0	1	0
A3	-1	0	0	0
A4	100	0	0	1
A5	33.33	3	1	2
A6	44.93	19	17	14
A7	52.5	19	22	20
A8	45.21	40	28	38
A9	60	13	15	24
A10	56	11	11	17

>TRINITY_DN36829_c0_g1_i2 from sample A6 / Example spliced isoform

GGCGACCGACAGAAGGTGATATGCTGGCGTGGACTTCAACGTGGGCACCCGCGTCCCTTCGCTACACCTATTACCGGCCTAGGGTTCCGGCTCACCGAAAAAAAGAAGGCGATGATAGCCTGTATTTGAATTTCCAAACCTAGCTGCCTCAGAGATCTAGAGTCAAACAACTGGACGGCTACCGGGCCCGGCACGCTGCT
CTGTTTGTTGGCGCGGCTTTTTGAGGCGTTGTAGGCTCTCTTGATAATTTGGTATCTTTTACTGCTCTTCCTTGTGCTCCAGCCGCCGCCTCCTGGTTTCGGCGAGGATTATGCAGCTAAGGAAATCCCAATTCCATCAATTGCTCAGGTCTCTACGGTATGATTGTCCTCCTTGCCAATTCTTGAAGATCTGTCAGCAG
CGGATTCAAATTCTTAAACGATATGGTGGGATATGCTCTGTGTCCCTCGCTTCCAAAACCAACATGGTTAGAGACACTGCTGACTGATAAATTCTTTGTTCCCTGTTCGAAGCATATTGAACAAAAGAAAAACGAAAGCAATATTTTTTGCATCGATTGTAGCAAAAGTATCTGTCAACACTGTCTTCCCAAGCATCGGT
CGCATCGGCTTCTTCAGGTGCGAAGATATGTGTACCACGACGTTATCCGGCTGCTAGACTTGCAGAAGCTTCTAGATTGCTCGCTAGTGCAGACTTATATCATTAATAGTGCGAAGGTCGTATTTTTGAACCAGCGGCCCCAGTCCAGGCTCCCCAAAGGACTGGGAAACTCTTGCCAAACTTGTGACAGAAGCCTGCAG
GAATCGTACTGTTATTGCTCGGTTTCGTGCAAGGTCACATGGCTGCCTCGGCTACTCTGTTCCCTTTTTTAAAACTATCATTATAATCATCCCATAAGTAAATTATAGCAATAATCTGCTCATGTTGCAATCACACTTTTACCGCTTCATTTTTAGCTTCTTTTTATAAAAATCAAGCTATACAATATTCAAGTAGGTAT
GGCATTGGGATTGATGGTGCAGGTAAATGCTGTGTTGAATAAAGAAAAGGATTTATCTACATTACTGTACCATTGCAGCTCACTTCCATTTCCTGATATTTTTTACTACTCTCCGAGAGAAATTTGTTACAAGAATAGAAAGTTTGCAGAAATGGGAGAAGAATTTTCGGCTAGCAGTGTGTTAGAAGAGACATGTTCAG
GCTCCAACGCTAACGATGTGCGTTGGATGTTGACATGTTCGGAGATAGACATGCCTTTAGTAAAGAAAACATGCAGGCGTCTTGCTGCACCCCACTCTATTGCCCGGACATCAACTAGCAGGAGAAAGGCGGAATTTTTGGGTTGGTCTGTTCTCGATGAGAGTCAGTTTACAAGGTTTTCTGTACTACAGAGTGCGTAT
ATTTAACGTCTATGTTAGGCGCCCAAAGATTATCATTACCCGTAGCATTACCTATTACACTGTTTTATATTCATCGATAGTGTTTTGATATTGTATATTTTGTTTGTCGTACATTGTGCATATCTTTCTAAATAGTCATCAAAATCAAACTAG

>TRINITY_DN36829_c0_g1_i9 from sample A6 / Example unspliced isoform:

GGTGGGATATGCTCTGTGTCCCTCGCTTCCAAAACCAACATGGTTAGAGACACTGCTGACTGATAAATTCTTTGTTCCCTGTTCGAAGCATATTGAACAAAAGAAAAACGAAAGCAATATTTTTTGCATCGATTGTAGCAAAAGTATCTGTCAACACTGTCTTCCCAAGCATCGGTCGCATCGGCTTCTTCAGGTGCGAA
GATATGTGTACCACGACGTTATCCGGCTGCTAGACTTGCAGAAGCTTCTAGATTGCTCGCTAGTGCAGGTGTGGCATTTGCTGTACTGAAATGAAGAATCGATTATCTGGATAAAAAACAATTTTGTGTCTAATTAATGGTTTTATTTGTTGGCCGGAAATTTGCAGACTTATATCATTAATAGTGCGAAGGTCGTATTT
TTGAACCAGCGGCCCCAGTCCAGGCTCCCCAAAGGACTGGGAAACTCTTGCCAAACTTGTGACAGAAGCCTGCAGGAATCGTACTGTTATTGCTCGGTTTCGTGCAAGGTAAATGCTGTGTTGAATAAAGAAAAGGATTTATCTACATTACTGTACCATTGCAGCTCACTTCCATTTCCTGATATTTTTTACTACTCTCC
GAGAGAAATTTGTTACAAGAATAGAAAGTTTGCAGAAATGGGAGAAGAATTTTCGGCTAGCAGTGTGTTAGAAGAGACATGTTCAGGCTCCAACGCTAACGATGTGCGTTGGATGTTGACATGTTCGGAGATAGACATGCCTTTAGTAAAGAAAACATGCAGGCGTCTTGCTGCACCCCACTCTATTGCCCGGACATCAA
CTAGCAGGAGAAAGGgtattccccacagatcgccaatatattaattaattatagtttatttttaataatttattttgaatgatgcttaacactccaagagatctgtattgtgcacaccagCGGAATTTTTGGGTTGGTCTGTTCTCGATGAGAGTCAGTTTACAAGGTTTTCTGTACTACAGAGTGCGTATATTTAACGT
CTATGTTAGGCGCCCAAAGATTATCATTACCCGTAGCATTACCTATTACACTGTTTTATATTCATCGATAGTGTTTTGATATTGTATATTTTGTTTGTCGTACATTGTGCATATCTTTCTAAATAGTCATCAAAATCAAACTAG

Intron 3 GTCACATGGC ... GATGGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.57	5	2	2
A2	0	1	0	0
A3	50	2	2	2
A4	0	1	0	0
A5	0	1	0	0
A6	41.67	7	4	6
A7	23.81	16	3	7
A8	34.33	22	2	21
A9	38.46	16	8	12
A10	50	8	15	1

>TRINITY_DN36829_c0_g1_i11 from sample A6 / Example spliced isoform

GGCGACCGACAGAAGGTGATATGCTGGCGTGGACTTCAACGTGGGCACCCGCGTCCCTTCGCTACACCTATTACCGGCCTAGGGTTCCGGCTCACCGAAAAAAAGAAGGCGATGATAGCCTGTATTTGAATTTCCAAACCTAGCTGCCTCAGAGATCTAGAGTCAAACAACTGGACGGCTACCGGGCCCGGCACGCTGCT
CTGTTTGTTGGCGCGGCTTTTTGAGGCGTTGTAGGCTCTCTTGATAATTTGGTATCTTTTACTGCTCTTCCTTGTGCTCCAGCCGCCGCCTCCTGGTTTCGGCGAGGATTATGCAGCTAAGGAAATCCCAATTCCATCAATTGCTCAGGTCTCTACGGTATGATTGTCCTCCTTGCCAATTCTTGAAGATCTGTCAGCAG
CGGATTCAAATTCTTAAACGATATGGTGGGATATGCTCTGTGTCCCTCGCTTCCAAAACCAACATGGTTAGAGACACTGCTGACTGATAAATTCTTTGTTCCCTGTTCGAAGCATATTGAACAAAAGAAAAACGAAAGCAATATTTTTTGCATCGATTGTAGCAAAAGTATCTGTCAACACTGTCTTCCCAAGCATCGGT
CGCATCGGCTTCTTCAGGTGCGAAGATATGTGTACCACGACGTTATCCGGCTGCTAGACTTGCAGAAGCTTCTAGATTGCTCGCTAGTGCAGACTTATATCATTAATAGTGCGAAGGTCGTATTTTTGAACCAGCGGCCCCAGTCCAGGCTCCCCAAAGGACTGGGAAACTCTTGCCAAACTTGTGACAGAAGCCTGCAG
GAATCGTACTGTTATTGCTCGGTTTCGTGCAAGGTAAATGCTGTGTTGAATAAAGAAAAGGATTTATCTACATTACTGTACCATTGCAGCTCACTTCCATTTCCTGATATTTTTTACTACTCTCCGAGAGAAATTTGTTACAAGAATAGAAAGTTTGCAGAAATGGGAGAAGAATTTTCGGCTAGCAGTGTGTTAGAAGA
GACATGTTCAGGCTCCAACGCTAACGATGTGCGTTGGATGTTGACATGTTCGGAGATAGACATGCCTTTAGTAAAGAAAACATGCAGGCGTCTTGCTGCACCCCACTCTATTGCCCGGACATCAACTAGCAGGAGAAAGGCGGAATTTTTGGGTTGGTCTGTTCTCGATGAGAGTCAGTTTACAAGGTTTTCTGTACTAC
AGAGTGCGTATATTTAACGTCTATGTTAGGCGCCCAAAGATTATCATTACCCGTAGCATTACCTATTACACTGTTTTATATTCATCGATAGTGTTTTGATATTGTATATTTTGTTTGTCGTACATTGTGCATATCTTTCTAAATAGTCATCAAAATCAAACTAG

>TRINITY_DN36829_c0_g1_i2 from sample A6 / Example unspliced isoform:

GGCGACCGACAGAAGGTGATATGCTGGCGTGGACTTCAACGTGGGCACCCGCGTCCCTTCGCTACACCTATTACCGGCCTAGGGTTCCGGCTCACCGAAAAAAAGAAGGCGATGATAGCCTGTATTTGAATTTCCAAACCTAGCTGCCTCAGAGATCTAGAGTCAAACAACTGGACGGCTACCGGGCCCGGCACGCTGCT
CTGTTTGTTGGCGCGGCTTTTTGAGGCGTTGTAGGCTCTCTTGATAATTTGGTATCTTTTACTGCTCTTCCTTGTGCTCCAGCCGCCGCCTCCTGGTTTCGGCGAGGATTATGCAGCTAAGGAAATCCCAATTCCATCAATTGCTCAGGTCTCTACGGTATGATTGTCCTCCTTGCCAATTCTTGAAGATCTGTCAGCAG
CGGATTCAAATTCTTAAACGATATGGTGGGATATGCTCTGTGTCCCTCGCTTCCAAAACCAACATGGTTAGAGACACTGCTGACTGATAAATTCTTTGTTCCCTGTTCGAAGCATATTGAACAAAAGAAAAACGAAAGCAATATTTTTTGCATCGATTGTAGCAAAAGTATCTGTCAACACTGTCTTCCCAAGCATCGGT
CGCATCGGCTTCTTCAGGTGCGAAGATATGTGTACCACGACGTTATCCGGCTGCTAGACTTGCAGAAGCTTCTAGATTGCTCGCTAGTGCAGACTTATATCATTAATAGTGCGAAGGTCGTATTTTTGAACCAGCGGCCCCAGTCCAGGCTCCCCAAAGGACTGGGAAACTCTTGCCAAACTTGTGACAGAAGCCTGCAG
GAATCGTACTGTTATTGCTCGGTTTCGTGCAAGgtcacatggctgcctcggctactctgttcccttttttaaaactatcattataatcatcccataagtaaattatagcaataatctgctcatgttgcaatcacacttttaccgcttcatttttagcttctttttataaaaatcaagctatacaatattcaagtaggtat
ggcattgggattgatggtgcagGTAAATGCTGTGTTGAATAAAGAAAAGGATTTATCTACATTACTGTACCATTGCAGCTCACTTCCATTTCCTGATATTTTTTACTACTCTCCGAGAGAAATTTGTTACAAGAATAGAAAGTTTGCAGAAATGGGAGAAGAATTTTCGGCTAGCAGTGTGTTAGAAGAGACATGTTCAG
GCTCCAACGCTAACGATGTGCGTTGGATGTTGACATGTTCGGAGATAGACATGCCTTTAGTAAAGAAAACATGCAGGCGTCTTGCTGCACCCCACTCTATTGCCCGGACATCAACTAGCAGGAGAAAGGCGGAATTTTTGGGTTGGTCTGTTCTCGATGAGAGTCAGTTTACAAGGTTTTCTGTACTACAGAGTGCGTAT
ATTTAACGTCTATGTTAGGCGCCCAAAGATTATCATTACCCGTAGCATTACCTATTACACTGTTTTATATTCATCGATAGTGTTTTGATATTGTATATTTTGTTTGTCGTACATTGTGCATATCTTTCTAAATAGTCATCAAAATCAAACTAG