sp|Q6NKX1|PROD2_ARATH ----> Proline dehydrogenase 2, mitochondrial

Intron 1 GCAAGTAACT ... GGTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.75	1023	282.6	123.3
A2	12.09	819	240.4	112.7
A3	9.94	1303	404.4	143.9
A4	8.66	773	247.3	73.3
A5	10.89	538	152.6	65.7
A6	11.76	1476	377.6	196.6
A7	10.63	3552	1054.9	422.4
A8	12.43	1831.1	457	260
A9	12.28	3054	813.1	427.6
A10	10.94	3432	997.3	421.7

>TRINITY_DN18668_c0_g1_i1 from sample A2 / Example spliced isoform

GATAGATGTTAGTGGTCCTCGTAAAATTTGGTTACACATGAAAGGACCTATCAACTTGGACGAAAGACCGGTGCCACGTCATAGCTTAAGGAGGTACGACCGCGTGATTTGAGTTCTACTAAATGGCAGCCTTTACCCTGTTTGTTCAATGAGATTTGTATGCCTCCTTCGCATAGCTTGAGAGCAGGAAATTAGGTGCC
TGGGACTGCGCCAGGCCAGTGAATCTTTTCAGTCCCAGAAGAGCAACCAACAGAGCGATTGCTTTGCCTTTCTTGAATCGGTTCAGCCTTTCTTGATCTCGAATTCAAGATTTGCAGATTTTCATGGCGTCCGTGGTGCGTTCATTTCTGAGCCGAAGAACCGGCAGAGTTTGGAGCTCAACCAGGCGCCATGAATTCTG
TTACACGAGTTACCGTAACATGAATTCGTCTTCAAAACCCCTGCAATATTCTTCCGTGGCTGCAGCCACGGCTCCGCAAATTCGTGTCAATGGAGCCATTAATGCTCCTGCTGCTTCTTCCATTCACGATTTCGAAGACGCGGAGGGATTGTTCTCGGGGTTAACCACGAGGGAGCTCGTTGCTTCGCTCTTCAATCTGC
TCCTCGTTTCCTGCGAGCCGGTCGCGGATCTCGGCATGAAGGTTTTGAAATCGCCTCTGATGGACTACGCGCCTTGCAGAGTGCCGATTCTGAAAGCTGTGAAGCGGACGGTGTTTTCTCATTTCTGCGCCGGAGAAACTGTCGCAGAGGCCTCGGACACTTTGACCAGAATGTGGGCGCTGGGTTTGAAGGGCATTCTT
GATTACAGCTTAGAAGATGCCGTCGATAACGCTGCCTGTGATGGCAATCTCCGCCAGTTTTTAAGAACTGTCGAATCGACACCTCTGCTTCCACCTGGATCGGTGAGTTTCGCGTGTGCGAAGATAACGGCCATCTGTCCTCTCGCTCTTTTGGAAAGAATCAGCCACTTGCTAAGATGGCAGCACCGCCATCCTACCTT
CAACCTGCCGTGGAAGGCAGAGGCTCTGCCAGTTCTTGCTCCATCAAGCCCTACTCACCACACATACTCTGCCCCTGAGCCATTGAGTCCCGCGGAGGAAAAGGATCTCATTTTAGCGGAGCAAAGGCTGTTTCAGCTCTGCCGAGCCTGCGAAGCCATTTCGCTTCCTTTGCTTGTAGATGCAGAATACACAGCTGTTC
AGCCTGCCATTGATTACTTTATTTACAGAGCATTTTCAGAATTCAACAGACGCCGTGAGCCTTTGGTGTATGGGACTATACAAGCTTACTTAAAAGACTCTTTGCCTCGCCTCATTGAGTCATCCGAAGCGGCTAAGAAGCAGGGCATTTCAATGGCTCTGAAACTTGTTCGTGGCGCCTACATCACCAGAGAAACTGCC
GAGGCGCAGTCAGTGGGCGCCCCTTCACCTGTACATTCTCTGATTACCGATACACACAGTTGTTTCAATGCCTGTACTTCACTGATGCTGGAGAAGGTTTCCAAGGGCGAAGCATCACTGGTACTCGCTACACACAATTTCGAGTCTGCAAAATTGGCCGCTGGTAAGGCTGAAGAATTGGGAATTATGAGAAGAGGACA
GGCCGGGTTACAGTTCGCTCAACTGAAGGGCATGTCGGACACTCTATCGCTGGGTTTGGTGAAAGCTGGATTCGAGGTGAGCAAATATCTTGCGTTTGGACCAGTGGAGCAAGTGCTCCCTTATCTCATCAGAAGAGCCGAAGAAAACCGTGGGATGTTCTCTACAACCCCTCTGGATATTCAGCGTCTCAGGGCGGAGT
TGAAACGGAGGATTTCACCATTTTGACAGCCAGATAAATAACCAAACTGCATACTGCAACAGAAGCTTTATCTCTGTCTGACTTTATGAGGACTGCAACAGAAACTTTATCTCTGTCTGACTTTATAAGGAAATGTTCTTCCTGTACACTGCCAACTGATGGATGGGATCTTTCATTTAGTGATAATGATGAAACCTGTA
ACTCTCTTCTGCTAACATGAAGGTGAAAAATTTGGCGTCCAATCTATAAATGCTTTTCTGCTCATCTGAAAATCAAGTAGAATTTGATTTCCAATCTGTAATGTTCTTTTGCTAATCTGAAAGTGAAGAATTTGATGGCCAATCTGCAATGCTATTTTACTCATATGAAAATGAGAAAAATTTGATGTCCAATTTCAATA
TGAAGAATGTTATGTTTGCCAAGTTTAAATGAATGAATGGTGAAAGCTATGTCATCAAATTCCATAAAATAACTTAAAATTGATGGGCGTTAATCGG

>TRINITY_DN18668_c0_g1_i2 from sample A2 / Example unspliced isoform:

ACCTCTGCTTCCACCTGGATCGgcaagtaactcatctctaactcgtgtcaaatctctaccaaattatttgtccgaaattgtttgctaaatccggcgtatttggtgttgcagGTGAGTTTCGCGTGTGCGAAGATAACGGCCATCTGTCCTCTCGCTCTTTTGGAAAGAATCAGCCACTTGCTAAGATGGCAGCACCGCCA
TCCTACCTTCAACCTGCCGTGGAAGGCAGAGGCTCTGCCAGTTCTTGCTCCATCAAGCCCTACTCACCACACATACTCTGCCCCTGAGCCATTGAGTCCCGCGGAGGAAAAGGATCTCATTTTAGCGGAGCAAAGGCTGTTTCAGCTCTGCCGAGCCTGCGAAGCCATTTCGCTTCCTTTGCTTGTAGATGCAGAATACA
CAGCTGTTCAGCCTGCCATTGATTACTTTATTTACAGAGCATTTTCAGAATTCAACAGACGCCGTGAGCCTTTGGTGTATGGGACTATACAAGCTTACTTAAAAGACTCTTTGCCTCGCCTCATTGAGTCATCCGAAGCGGCTAAGAAGCAGGGCATTTCAATGGCTCTGAAACTTGTTCGTGGCGCCTACATCACCAGA
GAAACTGCCGAGGCGCAGTCAGTGGGCGCCCCTTCACCTGTACATTCTCTGATTACCGATACACACAGTTGTTTCAATGCCTGTACTTCACTGATGCTGGAGAAGGTTTCCAAGGGCGAAGCATCACTGGTACTCGCTACACACAATTTCGAGTCTGCAAAATTGGCCGCTGGTAAGGCTGAAGAATTGGGAATTATGAG
AAGAGGACAGGCCGGGTTACAGTTCGCTCAACTGAAGGGCATGTCGGACACTCTATCGCTGGGTTTGGTGAAAGCTGGATTCGAGGTGAGCAAATATCTTGCGTTTGGACCAGTGGAGCAAGTGCTCCCTTATCTCATCAGAAGAGCCGAAGAAAACCGTGGGATGTTCTCTACAACCCCTCTGGATATTCAGCGTCTCA
GGGCGGAGTTGAAACGGAGGATTTCACCATTTTGACAGCCAGATAAATAACCAAACTGCATACTGCAACAGAAGCTTTATCTCTGTCTGACTTTATGAGGACTGCAACAGAAACTTTATCTCTGTCTGACTTTATAAGGAAATGTTCTTCCTGTACACTGCCAACTGATGGATGGGATCTTTCATTTAGTGATAATGATG
AAACCTGTAACTCTCTTCTGCTAACATGAAGGTGAAAAATTTGGCGTCCAATCTATAAATGCTTTTCTGCTCATCTGAAAATCAAGTAGAATTTGATTTCCAATCTGTAATGTTCTTTTGCTAATCTGAAAGTGAAGAATTTGATGGCCAATCTGCAATGCTATTTTACTCATATGAAAATGAGAAAAATTTGATGTCCA
ATTTCAATATGAAGAATGTTATGTTTGCCAAGTTTAAATGAATGAATGGTGAAAGCTATGTCATCAAATTCCATAAAATAACTTAAAATTGATGGGCGTTAATCGG

Intron 2 GCAAGTATCT ... TTTAACGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.82	1787	470	0
A2	22.15	1265	360	0
A3	23.9	1420	446	0
A4	20.62	1186	308	0
A5	25.31	475	161	0
A6	25.81	3096	1077	3
A7	25.61	6332	2180	3
A8	22.81	6823	2016	2
A9	24.86	5717	1891	1
A10	27.35	4934	1857	3

>TRINITY_DN37031_c0_g1_i1 from sample A8 / Example spliced isoform

CGGATAGATGTTAGTGGTCCTCGTAAAATTTGGTTACACATGAAAGGACCTATCAACTTGGACGAAAGACCGGTGCCACGTCATAGCTTAAGGAGGTACGACCGCGTGATTTGAGTTCTACTAAATGGCAGCCTTTACCCTGTTTGTTCAATGAGATTTGTATGCCTCCTTCGCATAGCTTGAGAGCAGGAAATTAGGTG
CCTGGGACTGCGCCAGGCCAGTGAATCTTTTCAGTCCCAGAAGAGCAACCAACAGAGCGATTGCTTTGCCTTTCTTGAATCGGTTCAGCCTTTCTTGATCTCGAATTCAAGATTTGCAGATTTTCATGGCGTCCGTGGTGCGTTCATTTCTGAGCCGAAGAACCGGCAGAGTTTGGAGCTCAACCAGGCGCCATGAATTC
TGTTACACGAGTTACCGTAACATGAATTCGTCTTCAAAACCCCTGCAATATTCTTCCGTGGCTGCAGCCACGGCTCCGCAAATTCGTGTCAATGGAGCCATTAATGCTCCTGCTGCTTCTTCCATTCACGATTTCGAAGACGCGGAGGGATTGTTCTCGGGGTTAACCACGAGGGAGCTCGTTGCTTCGCTCTTCAATCT
GCTCCTCGTTTCCTGCGAGCCGGTCGCGGATCTCGGCATGAAGGTTTTGAAATCGCCTCTGATGGACTACGCGCCTTGCAGAGTGCCGATTCTGAAAGCTGTGAAGCGGACGGTGTTTTCTCATTTCTGCGCCGGAGAAACTGTCGCAGAGGCCTCGGACACTTTGACCAGAATGTGGGCGCTGGGTTTGAAGGGCATTC
TTGATTACAGCTTAGAAGATGCCGTCGATAACGCTGCCTGTGATGGCAATCTCCGCCAGTTTTTAAGAACTGTCGAATCGACACCTCTGCTTCCACCTGGATCGGTGAGTTTCGCGTGTGCGAAGATAACGGCCATCTGTCCTCTCGCTCTTTTGGAAAGAATCAGCCACTTGCTAAGATGGCAGCACCGCCATCCTACC
TTCAACCTGCCGTGGAAGGCAGAGGCTCTGCCAGTTCTTGCTCCATCAAGCCCTACTCACCACACATACTCTGCCCCTGAGCCATTGAGTCCCGCGGAGGAAAAGGATCTCATTTTAGCGGAGCAAAGGCTGTTTCAGCTCTGCCGAGCCTGCGAAGCCATTTCGCTTCCTTTGCTTGTAGATGCAGAATACACAGCTGT
TCAGCCTGCCATTGATTACTTTATTTACAGAGCATTTTCAGAATTCAACAGACGCCGTGAGCCTTTGGTGTATGGGACTATACAAGCTTACTTAAAAGACTCTTTGCCTCGCCTCATTGAGTCATCCGAAGCGGCTAAGAAGCAGGGCATTTCAATGGCTCTGAAACTTGTTCGTGGCGCCTACATCACCAGAGAAACTG
CCGAGGCGCAGTCAGTGGGCGCCCCTTCACCTGTACATTCTCTGATTACCGATACACACAGTTGTTTCAATGCCTGTACTTCACTGATGCTGGAGAAGGTTTCCAAGGGCGAAGCATCACTGGTACTCGCTACACACAATTTCGAGTCTGCAAAATTGGCCGCTGGTAAGGCTGAAGAATTGGGAATTATGAGAAGAGGA
CAGGCCGGGTTACAGTTCGCTCAACTGAAGGGCATGTCGGACACTCTATCGCTGGGTTTGGTGAAAGCTGGATTCGAGGTGAGCAAATATCTTGCGTTTGGACCAGTGGAGCAAGTGCTCCCTTATCTCATCAGAAGAGCCGAAGAAAACCGTGGGATGTTCTCTACAACCCCTCTGGATATTCAGCGTCTCAGGGCGGA

GTTGAAACGGAGGATTTCACCATTTTGACAGCCAGATAAATAACCAAACTGCATACTGCAACAGAAGCTTTATCTCTGTCTGACTTTATGAGGACTGCAACAGAAACTTTATCTCTGTCTGACTTTATAAGGAAATGTTCTTCCTGTACACTGCCAACTGATGGATGGGATCTTTCATTTAGTGATAATGATGAAACCTG
TAACTCTCTTCTGCTAACATGAAGGTGAAAAATTTGGCGTCCAATCTATAAATGCTTTTCTGCTCATCTGAAAATCAAGTAGAATTTGATTTCCAATCTGTAATGTTCTTTTGCTAATCTGAAAGTGAAGAATTTGATGGCCAATCTGCAATGCTATTTTACTCATATGAAAATGAGAAAAATTTGATGTCCAATTTCAA
TATGAAGAATGTTATGTTTGCCAAG

>TRINITY_DN37031_c0_g1_i2 from sample A8 / Example unspliced isoform:

CGCTACACACAATTTCGAGTCTGGTAAGCTCTCATTCTCATAATTTTTATTATAAAGAATACATTCAATGATAAGGGGATTTGATTTTTATTTATTGGTAATGGTAACAATTTATCAGCAAAATTGGCCGCTGGTAAGGCTGAAGAATTGGGAATTATGAGAAGAGGACAGGCCGGGTTACAGTTCGCTCAACTGAAGGG
CATGTCGGACACTCTATCGCTGGGTTTGGTGAAAGCTGGATTCGAGGTGAGCAAATATCTTGCGTTTGGACCAGTGGAGCAAGTGCTCCCTTATCTCATCAGAAGAGCCGAAGAAAACCGTGGGATGTTCTCTACAACCCCTCTGGATATTCAGCGTCTCAGgcaagtatctcagctaattcgtattcatttattttctt
attatattcttattcttattatggttaccaactggattttaacgcagGGCGGAGTTGAAACGGAG

Intron 3 GTAAGCTCTC ... AATTTATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.32	1254	0	403
A2	22.85	861	0	255
A3	23.82	1017	0	318
A4	23.83	844	3	264
A5	22.98	466	1	139
A6	24.01	1475	0	466
A7	23.98	3369	2	1063
A8	27.13	3401	0	1266
A9	24.31	3235	2	1039
A10	23.18	2760	0	833

>TRINITY_DN37622_c0_g1_i3 from sample A6 / Example spliced isoform

CCCGGAGATTTGTATGCCTCCTTCGCATAGCTTGAGAGCAGGAAATTAGGTGCCTGGGACTGCGCCAGGCCAGTGAATCTTTTCAGTCCCAGAAGAGCAACCAACAGAGCGATTGCTTTGCCTTTCTTGAATCGGTTCAGCCTTTCTTGATCTCGAATTCAAGATTTGCAGATTTTCATGGCGTCCGTGGTGCGTTCATT
TCTGAGCCGAAGAACCGGCAGAGTTTGGAGCTCAACCAGGCGCCATGAATTCTGTTACACGAGTTACCGTAACATGAATTCGTCTTCAAAACCCCTGCAATATTCTTCCGTGGCTGCAGCCACGGCTCCGCAAATTCGTGTCAATGGAGCCATTAATGCTCCTGCTGCTTCTTCCATTCACGATTTCGAAGACGCGGAGG
GATTGTTCTCGGGGTTAACCACGAGGGAGCTCGTTGCTTCGCTCTTCAATCTGCTCCTCGTTTCCTGCGAGCCGGTCGCGGATCTCGGCATGAAGGTTTTGAAATCGCCTCTGATGGACTACGCGCCTTGCAGAGTGCCGATTCTGAAAGCTGTGAAGCGGACGGTGTTTTCTCATTTCTGCGCCGGAGAAACTGTCGCA
GAGGCCTCGGACACTTTGACCAGAATGTGGGCGCTGGGTTTGAAGGGCATTCTTGATTACAGCTTAGAAGATGCCGTCGATAACGCTGCCTGTGATGGCAATCTCCGCCAGTTTTTAAGAACTGTCGAATCGACACCTCTGCTTCCACCTGGATCGGCAAGTAACTCATCTCTAACTCGTGTCAAATCTCTACCAAATTA
TTTGTCCGAAATTGTTTGCTAAATCCGGCGTATTTGGTGTTGCAGGTGAGTTTCGCGTGTGCGAAGATAACGGCCATCTGTCCTCTCGCTCTTTTGGAAAGAATCAGCCACTTGCTAAGATGGCAGCACCGCCATCCTACCTTCAACCTGCCGTGGAAGGCAGAGGCTCTGCCAGTTCTTGCTCCATCAAGCCCTACTCA
CCACACATACTCTGCCCCTGAGCCATTGAGTCCCGCGGAGGAAAAGGATCTCATTTTAGCGGAGCAAAGGCTGTTTCAGCTCTGCCGAGCCTGCGAAGCCATTTCGCTTCCTTTGCTTGTAGATGCAGAATACACAGCTGTTCAGCCTGCCATTGATTACTTTATTTACAGAGCATTTTCAGAATTCAACAGACGCCGTG
AGCCTTTGGTGTATGGGACTATACAAGCTTACTTAAAAGACTCTTTGCCTCGCCTCATTGAGTCATCCGAAGCGGCTAAGAAGCAGGGCATTTCAATGGCTCTGAAACTTGTTCGTGGCGCCTACATCACCAGAGAAACTGCCGAGGCGCAGTCAGTGGGCGCCCCTTCACCTGTACATTCTCTGATTACCGATACACAC
AGTTGTTTCAATGCCTGTACTTCACTGATGCTGGAGAAGGTTTCCAAGGGCGAAGCATCACTGGTACTCGCTACACACAATTTCGAGTCTGCAAAATTGGCCGCTGGTAAGGCTGAAGAATTGGGAATTATGAGAAGAGGACAGGCCGGGTTACAGTTCGCTCAACTGAAGGGCATGTCGGACACTCTATCGCTGGGTTT
GGTGAAAGCTGGATTCGAGGTGAGCAAATATCTTGCGTTTGGACCAGTGGAGCAAGTGCTCCCTTATCTCATCAGAAGAGCCGAAGAAAACCGTGGGATGTTCTCTACAACCCCTCTGGATATTCAGCGTCTCAGGGCGGAGTTGAAACGGAGGATTTCACCATTTTGACAGCCAGATAAATAACCAAACTGCATACTGC
AACAGAAGCTTTATCTCTGTCTGACTTTATGAGGACTGCAACAGAAACTTTATCTCTGTCTGACTTTATAAGGAAATGTTCTTCCTGTACACTGCCAACTGATGGATGGGATCTTTCATTTAGTGATAATGATGAAACCTGTAACTCTCTTCTGCTAACATGAAGGTGAAAAATTTGGCGTCCAATCTATAAATGCTTTT
CTGCTCATCTGAAAATCAAGTAGAATTTGATTTCCAATCTGTAATGTTCTTTTGCTAATCTGAAAGTGAAGAATTTGATGGCCAATCTGCAATGCTATTTTACTCATATGAAAATGAGAAAAATTTGATGTCCAATTTCAATATGAAGAATGTTATGTTTGCCAAGTTTAAATGAATGAATGGTGAAAGCTATGTCATCA
AATTC

>TRINITY_DN37622_c0_g1_i1 from sample A6 / Example unspliced isoform:

CGCTACACACAATTTCGAGTCTGgtaagctctcattctcataatttttattataaagaatacattcaatgataaggggatttgatttttatttattggtaatggtaacaatttatcagCAAAATTGGCCGCTGGTAAGGCTGAAGAATTGGGAATTATGAGAAGAGGACAGGCCGGGTTACAGTTCGCTCAACTGAAGGG
CATGTCGGACACTCTATCGCTGGGTTTGGTGAAAGCTGGATTCGAGGTGAGCAAATATCTTGCGTTTGGACCAGTGGAGCAAGTGCTCCCTTATCTCATCAGAAGAGCCGAAGAAAACCGTGGGATGTTCTCTACAACCCCTCTGGATATTCAGCGTCTCAGGGCGGAGTTGAAACGGAGGATTTCACCATTTTGACAGC
CAGATAAATAACCAAACTGCATACTGCAACAGAAGCTTTATCTCTGTCTGACTTTATGAGGACTGCAACAGAAACTTTATCTCTGTCTGACTTTATAAGGAAATGTTCTTCCTGTACACTGCCAACTGATGGATGGGATCTTTCATTTAGTGATAATGATGAAACCTGTAACTCTCTTCTGCTAACATGAAGGTGAAAAA
TTTGGCGTCCAATCTATAAATGCTTTTCTGCTCATCTGAAAATCAAGTAGAATTTGATTTCCAATCTGTAATGTTCTTTTGCTAATCTGAAAGTGAAGAATTTGATGGCCAATCTGCAATGCTATTTTACTCATATGAAAATGAGAAAAATTTGATGTCCAATTTCAATATGAAGAATGTTATGTTTGCCAAGTTTAAAT
GAATGAATGGTGAAAGCTATGTCATCAAATTC