sp|P0CG83|UBIQP_HORVU ----> Polyubiquitin (Fragment)

Intron 1 GTTCTGTATC ... CACTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.94	117	14	50
A2	27.59	105	18	40
A3	29.82	120	19	51
A4	33.77	153	33	78
A5	31.36	81	6	37
A6	23.54	536	33	165
A7	22.27	597	45	171
A8	25.31	298	14	101
A9	21.93	452	28	127
A10	24.9	543	48	180

>TRINITY_DN47715_c2_g5_i1 from sample A10 / Example spliced isoform

TTTCGAAATACAAAAAGAATTCAATGTTTGACCCGCCGACCTCAACGCCTAGACATTACTGGATTCTTCCGGAGATTGTGGTTGAAATTTGGAAGGGGCGTCCGAGAATGCATATAAATAACGATCTGCCCTCCTATTCTTTTCATTGTGATATTTCATTCAATTCCCTTCATTGCGATTTCGATCAAGTTCTGTAAATC
CTCGGCGAATCAACCAAACTCTTTCTTCTCGTCTCTGCTCGCATTTCTTTCAGGTGTTGCGTTTGTAAAATTTGGATTTCAAGATGCAGATATTCGTGAAGACGTTGACCGGAAAGACTATAACTCTTGAGGTGGAGA

>TRINITY_DN47715_c2_g5_i3 from sample A10 / Example unspliced isoform:

TTTCGAAATACAAAAAGAATTCAATGTTTGACCCGCCGACCTCAACGCCTAGACATTACTGGATTCTTCCGGAGATTGTGGTTGAAATTTGGAAGGGGCGTCCGAGAATGCATATAAATAACGATCTGCCCTCCTATTCTTTTCATTGTGATATTTCATTCAATTCCCTTCATTGCGATTTCGATCAAGTTCTGTAAATC
CTCGGCGAATCAACCAAACTCTTTCTTCTCGTCTCTGCTCGCATTTCTTTCAGgttctgtatcgtaagccgccatgaattctatcgtcttttcggatttttgtgctttatatctgtttatattttaggctgtccaaattctttgttttgaattttcgatttgagtgtttttcacatttgattttcgtataaactgcaaga
aacgtattattatccttttgccaatcgacaattggttaccctatgaatttttttcgtttccctggtccgtatgttgtatgagggttttctatggagcgtagggtttcattgtctgtattgtgaggttctggggttatgtggagtctctaatggcgcactaccactgtgcagGTGTTGCGTTTGTAAAATTTGGATTTCAA
GATGCAGATATTCGTGAAGACGTTGACCGGAAAGACTATAACTCTTGAGGTGGAGAACTCAGATACCATTGACAACGTCAAGGCGAAGATTCAGGACAAAGA

Intron 2 GTACTGTATC ... CACTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.3	160.5	12.5	66
A2	26.14	144	19	50
A3	27.88	170	18.5	64
A4	30.77	197.5	34.5	85.5
A5	28.66	109	7.5	42.5
A6	24.3	770	37	250.5
A7	21.98	824	48.5	231
A8	24.38	433	19	137.5
A9	22.72	644	35.5	192
A10	24.5	748.5	53.5	241.5

>TRINITY_DN103612_c1_g1_i1 from sample A4 / Example spliced isoform

CAATGTTTGACCCGCCGACCTCAACGCCTAGACATTACTGGATTCTTCCGGAGATTGGTTGAAATTTGGAAGGGGCGTCCGAGAATGCATATAAATAACGATCTGCCCCCCTCTTCCTTTCATTGTGATATTTCATTCAGTTCTCTTCATTGCGATTTCAATCAAGTTCTGTAAATCCTCGGCGAATCAACCAAACTTTT
TCTTCTCGTCTCTGCTCACATTTCTTTCAGGTGTTGCGTTTGTAAAATTTGGATTTCAAGATGCAGATATTCGTGAAGACGTTGACCGGAAAGACTATAACTCTTGAGGTGGAGAGCTCAGATACCATTGACAACGTCAAGGCGAAGATTCAGGACAAAGAGGGAATCCCCCCTGATCAACAGAGGCTCATATTTGCTGG
CAAGCAGTTGGAAGATGGCCGAACCCTGGCCGATTACAATATTCAGAAGGAATCCACTCTTCACCTCGTCCTCCGTCTCAGAGGTGGAATGCAGATCTTTGTCAAAACCCTCACTGGCAAGACCATCACGCTCGAAGTTGAGAGCTCCGATACTATTGACAATGTAAAGGCCAAAATTCAAGACAAGGAGGGTATTCCAC
CCGACCAGCAGAGGTTGATCTTTGCCGGAAAACAGCTCGAGGACGGACGCACTCTTGCCGATTACAACATCCAGAAGGAATCCACTCTTCATCTTGTACTCCGTCTCAGGGGAGGGATGCAAATCTTTGTGAAGACCCTGACCGGCAAGACCATCA

>TRINITY_DN103612_c1_g1_i3 from sample A4 / Example unspliced isoform:

CAATGTTTGACCCGCCGACCTCAACGCCTAGACATTACTGGATTCTTCCGGAGATTGGTTGAAATTTGGAAGGGGCGTCCGAGAATGCATATAAATAACGATCTGCCCCCCTCTTCCTTTCATTGTGATATTTCATTCAGTTCTCTTCATTGCGATTTCAATCAAGTTCTGTAAATCCTCGGCGAATCAACCAAACTTTT
TCTTCTCGTCTCTGCTCACATTTCTTTCAGgtactgtatcgtaagccgctatgaattctatcgtcttttcggatttttgtgctttatatctgtttatattttaggctgtccaaattctttgttttgaattttcgattggagtatttttcacatttgattaccctatgtattattatccttttgccgatcgacaattggtt
accctatgaattttttttgtttccctggtccgtatgttgtatgagggttttctatggagcgtagggtttcattgtctgtattgtgaggttctggggttatgtggagtctctaatggcgcactaccactgtgcagGTGTTGCGTTTGTAAAATTTGGATTTCAAGATGCAGATATTCGTGAAGACGTTGACCGGAAAGACT
ATAACTCTTGAGGTGGAGAGCTCAGATACCATTGACAACGTCAAGGCGAAGATTCAGGACAAAGAGGGAATCCCCCCTGATCAACAGAGGCTCATATTTGCTGGCAAGCAGTTGGAAGATGGCCGAACCCTGGCCGATTACAATATTCAGAAGGAATCCACTCTTCACCTCGTCCTCCGTCTCAGAGGTGGAATGCAGAT
CTTTGTCAAAACCCTCACTGGCAAGACCATCACTCTGGAAGTTGAGAGCTCTGACACAATTGACAATGT

Intron 3 GTACTGTATC ... CACTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.67	204	11	82
A2	24.69	183	20	60
A3	25.93	220	18	77
A4	27.76	242	36	93
A5	25.95	137	9	48
A6	25.07	1004	41	336
A7	21.68	1051	52	291
A8	23.45	568	24	174
A9	23.51	836	43	257
A10	24.11	954	59	303

>TRINITY_DN103612_c1_g1_i1 from sample A4 / Example spliced isoform

CAATGTTTGACCCGCCGACCTCAACGCCTAGACATTACTGGATTCTTCCGGAGATTGGTTGAAATTTGGAAGGGGCGTCCGAGAATGCATATAAATAACGATCTGCCCCCCTCTTCCTTTCATTGTGATATTTCATTCAGTTCTCTTCATTGCGATTTCAATCAAGTTCTGTAAATCCTCGGCGAATCAACCAAACTTTT
TCTTCTCGTCTCTGCTCACATTTCTTTCAGGTGTTGCGTTTGTAAAATTTGGATTTCAAGATGCAGATATTCGTGAAGACGTTGACCGGAAAGACTATAACTCTTGAGGTGGAGAGCTCAGATACCATTGACAACGTCAAGGCGAAGATTCAGGACAAAGAGGGAATCCCCCCTGATCAACAGAGGCTCATATTTGCTGG
CAAGCAGTTGGAAGATGGCCGAACCCTGGCCGATTACAATATTCAGAAGGAATCCACTCTTCACCTCGTCCTCCGTCTCAGAGGTGGAATGCAGATCTTTGTCAAAACCCTCACTGGCAAGACCATCACGCTCGAAGTTGAGAGCTCCGATACTATTGACAATGTAAAGGCCAAAATTCAAGACAAGGAGGGTATTCCAC
CCGACCAGCAGAGGTTGATCTTTGCCGGAAAACAGCTCGAGGACGGACGCACTCTTGCCGATTACAACATCCAGAAGGAATCCACTCTTCATCTTGTACTCCGTCTCAGGGGAGGGATGCAAATCTTTGTGAAGACCCTGACCGGCAAGACCATCA

>TRINITY_DN103612_c1_g1_i7 from sample A4 / Example unspliced isoform:

CAATGTTTGACCCGCCGACCTCAACGCCTAGACATTACTGGATTCTTCCGGAGATTGGTTGAAATTTGGAAGGGGCGTCCGAGAATGCATATAAATAACGATCTGCCCCCCTCTTCCTTTCATTGTGATATTTCATTCAGTTCTCTTCATTGCGATTTCAATCAAGTTCTGTAAATCCTCGGCGAATCAACCAAACTTTT
TCTTCTCGTCTCTGCTCACATTTCTTTCAGgtactgtatcgtaagccgctatgaattctatcgtcttttcggatttttgtgctttatatctgtttatattttaggctgtccaaattctttgttttgaattttcgattggagtatttttcacattggattttcgtataaactgcaagaaacgtattattatccttttgccg
atcgacaattggttaccctatgaatttttttttgtttccctggtccgtatgttgtatgagggttttctatggagcgtagggtttcattgtctgtattgtgaggttctggggttatgtggagtctctaatggcgcactaccactgtgcagGTGTTGCGTTTGTAAAATTTGGATTTCAAGATGCAGATATTCGTGAAGACG
TTGACCGGAAAGACTATAACTCTTGAGGTGGAGAGCTCAGATACCATTGACAACGTCAAGGCGAAGATTCAGGACAAAGAGGGAATCCCCCCTGATCAACAGAGGCTCATATTTGCTGGCAAGCAGTTGGAAGATGGCCGAACCCTGGCCGATTACAATATTCAGAAGGAATCCACTCTTCACCTCGTCCTCCGTCTCAG
AGGTGGAATGCAGATCTTTGTCAAAACCCTCACTGGCAAGACCATCACTCTGGAAGTTGAGAGCTCTGACACAATTGACAATGT