sp|Q84UV8|NEC3_NICLS ----> Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3

Intron 1 GTAAGAAAAA ... TAACGAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	74.68	30	109	68
A2	78.15	13	60	33
A3	65.62	33	59	67
A4	71.21	19	54	40
A5	80.28	7	36	21
A6	56.76	16	16	26
A7	67.54	31	69	60
A8	61.54	30	35	61
A9	45.95	40	25	43
A10	50.41	61	47	77

>TRINITY_DN43485_c0_g1_i5 from sample A6 / Example spliced isoform

CTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGATGGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCAGGTCTCTTCATGAGGCTGTGTGGTCTGGTTTTTCAGGGTTTGAAGCGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTTTCTTATTTGTGGTCGTTACAT
GGGAAGCCTGAGACCTGAGAGGGAGGCCGAGGGTGACAATGGAGTTGGGCGATTTCTTCTTTTCAGCATATGAAACCTGATAAGTGAGAGGAAGGCCGAGGGTGACAATGGAGTTGGGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTGCAAAA
TAAAGTACGGAAAAGTTAATGGATGGAATCTTGGCCACTGCGAATAAGGAACCGCCGGAGGAACCTCTGGGCATAGTGAATCCGAATCACATACAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAGTAAAGACAGTGTCGGAAAACCAATCGATGGCTCTCAAAGCATCTGTG
CATGATGT

>TRINITY_DN43485_c0_g1_i3 from sample A6 / Example unspliced isoform:

CTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGATGGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCAGGTCTCTTCATGAGGCTGTGTGGTCTGGTTTTTCAGGGTTTGAAGCGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTTTCTTATTTGTGGTCGTTACAT
GGGAAGCCTGAGACCTGAGAGGGAGGCCGAGGGTGACAATGGAGTTGGGCGATTTCTTCTTTTCAGCATATGAAACCTGATAAGTGAGAGGAAGGCCGAGGGTGACAATGGAGTTGGGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTGCAAAA
TAAAGTACGGAAAAGgtaagaaaaaaaaacctgatttctaagatgttccttttcgaccgttgcctgagttttcaaattcaaggtcagtttatgaccagaggtttgaatagatggttctgaggcttcacccaaaatgtgatgtagaagtgttagatctattccaatagtacacagtcgttatcatggatacatagttcatt
tatagaagggtctctaatcctctgaggaagttaggtttttatcctttcagcaggttaggattctgttttgatttctttttgatagttgtgatagatcctaactgaggttaaatttagctaatcttgcttttgtttaagacaaaatatatgttaagctttcatggatacatagttcagtgatggaagggcttctaatagat
ttgtgatttgatttctttctaatcctcttagtaccctatttcgattcttttgcaccaaatcaatctggtgcaccaggcggaggacgtcgaaattgtcgtcattggaatcatttacgagcttggccagcctcacgatttcttggcaaaggtatatttctggggagattatgatgtgatgatagtttgctaacaattttttt
ggtataacgaacagTTAATGGATGGAATCTTGGCCACTGCGAATAAGGAACCGCCGGAGGAACCTCTGGGCATAGTGAATCCGAATCACATACAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAGTAAAGACAGTGTCGGAAAACCAATCGATGGCTCTCAAAGCATCTGTGC
ATGATGT

Intron 2 GTATTTTCAT ... CCGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.59	17.5	46	38
A2	76.75	3.5	13	10
A3	74.16	7.5	28	15
A4	80.03	10	47	33
A5	90.22	2	14	22
A6	46.53	11.5	13	6
A7	41.62	22.5	25	7
A8	34.05	21.5	17	5
A9	60.98	4.5	6	8
A10	40.66	22	18	12

>TRINITY_DN42503_c0_g1_i15 from sample A8 / Example spliced isoform

TTTCCAACGATTTTAAATTTTTATATTACATTCTATATTTAGGAAGGCGGTGAATTCCTTATCATTCTTTCAACTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGAGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTC
AGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGT
TGGCACCATCTCCAAAATAAAGTACGGAAAAGCTAATGGATGTAATCTTGGCCACTGCGAATAAGGAACCGCCGGAGGAACCTCTGGGCATAGTGAATCCGAATCACATACAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAGTGAGTCATTTGGACAGTGATTTACGAGGTA
AAGACAGTGTCGGAAGACCAATCGATGGCTCTCAAAGCATCTGTGCATGATGCTCGGCTGCATTTTCCTTGTGAGTCGTATCTGCCGAAGCTGAGCCGTAGCTGCCGAAGCTGAGTCGTATCTGCCGAAGCTGCCAAGGAAAGTCTGACGAAGCACCCC

>TRINITY_DN42503_c0_g1_i20 from sample A8 / Example unspliced isoform:

TTTCCAACGATTTTAAATTTTTATATTACATTCTATATTTAGGAAGGCGGTGAATTCCTTATCATTCTTTCAACTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGAGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTC
AGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGT
TGGCACCATCTCCAAAATAAAGTACGGAAAAGCTAATGGATGTAATCTTGGCCACTGCGAATAAGGAACCGCCGGAAAAACCTCTGGGCATAGTGAATCCGAATCACATATAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAGTGAGTCATTTGGACAGTGATTTACGAGgta
ttttcatcgccttaccacaaatctgcgtccataggtattgaccctgtgacctgatacccttgattaaatcaatccgtgtgcagGTAAAGACAGTGTCGGAAGACCAATCGATGGCTCTCAAAGCATCTGTGCATGATGTAAGTACTGGAAATATAGTTTTCTGAAATTACAAATTACATGGCTGCCTTCAAACTATTCTA
GTAGTTGATGCAAGACGGAAAGCTGAATTCGAACCTCCTAAGTTTGGCAGCTCAAACCCTATATATGTCATGCTGCTCGATAGATCTATTCATCAGATCTATTGGACACCTTGCCTTCAACACTGTTGGGCAACATGATCTTCAGTACAGACATGTCGGATCGTCATTCTCGAACTTGTCAGGATTTGAAAGGAAGTATT
TCCCAGCTGATGCAGCTTCATGCAATTCCTAATTTGGGGGACTTCTTTCCCTTTCTTTAGTTTTCGGATCCTCAGGGTATCATTCGTACACCACCGC

Intron 3 GTGAGTCATT ... GATTTACGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.62	18.2	23	15.1
A2	54.05	11.6	9.4	4.6
A3	46.45	16.4	8.4	7.6
A4	65.97	9.1	14.1	8.5
A5	50.03	5.2	3.8	3.7
A6	53.76	36.4	16.9	17.5
A7	44.42	68.9	27.3	27.7
A8	41.13	92.9	27.2	25
A9	35.87	71.3	16.1	10.4
A10	52.48	54.6	31.9	30.2

>TRINITY_DN42503_c0_g1_i12 from sample A8 / Example spliced isoform

ATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAGTAAAGACAGTGTCGGAAGACCAATCGATGGCTCTCAAAGAATCTGTGCATGATATCTATTGGACACCTTGCCTTCAACACCGTTGGGCAACGTTGATCTTCACTACAGACATGTCGGATCGTCATTCTCGAACTTGTCAGGATTTGA
AAGGAAGTATTTCCCAGCTGATGCAGCTTCATGTAATTCCTAATTTGGGGGACTTCTTTCCCTTTCTTTAGTTTTCGGATCCTCAGGGTATCATTCGTACACCACCGCAATAGGCCATTCTTGATCAATTCTTTGAGGATCGTCTCGCCATCAGAGTAAAGGGAGCGAGAGAAGTTATGGCGAAAAGGATCTATTGGCCG
TTCTGCTGGACATGAGAAGTAAAGAATTTACCATTAGTGGCATGAGAGCCTACCTAATTGTAAGTCATTCGTTCTATCTTAATTGTAAGTCATTAGTTTACTTGAGTTTATCAGGCGTAGGATGTTATCTTTCATGGCAATAACGGATGATTCATTAGGAACAAAAACTAGAAGTGATTATGTAGATGATGCATTTACCT
TC

>TRINITY_DN42503_c0_g1_i15 from sample A8 / Example unspliced isoform:

TTTCCAACGATTTTAAATTTTTATATTACATTCTATATTTAGGAAGGCGGTGAATTCCTTATCATTCTTTCAACTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGAGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTC
AGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGT
TGGCACCATCTCCAAAATAAAGTACGGAAAAGCTAATGGATGTAATCTTGGCCACTGCGAATAAGGAACCGCCGGAGGAACCTCTGGGCATAGTGAATCCGAATCACATACAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAgtgagtcatttggacagtgatttacgagGTA
AAGACAGTGTCGGAAGACCAATCGATGGCTCTCAAAGCATCTGTGCATGATGCTCGGCTGCATTTTCCTTGTGAGTCGTATCTGCCGAAGCTGAGCCGTAGCTGCCGAAGCTGAGTCGTATCTGCCGAAGCTGCCAAGGAAAGTCTGACGAAGCACCCC

Intron 4 GTGAGTCATT ... CCGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	58.62	30	47	38
A2	40.85	21	19	10
A3	46.99	22	24	15
A4	66.04	18	37	33
A5	82.61	4	16	22
A6	20.75	42	16	6
A7	18.42	93	35	7
A8	13.97	117	33	5
A9	10.53	102	16	8
A10	22.4	71	29	12

>TRINITY_DN42503_c0_g1_i7 from sample A8 / Example spliced isoform

ATGTAATCTTGGGCACTGCGAATAAGGAACCGCCGGAAAAACCTCTGGGCATAGTGAATCCGAATCACATATAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAGTAAAGACAGTGTCGGAAGACCAATCGATGGCTCTCAAAGCATCTGTGCATGATGTAAGTACTGGAAATA
TAGTTTTCTGAAATTACAAATTACATGGCTGCCTTCAAACTATTCTAGTAGTTGATGCAAGACGGAAAGCTGAATTCGAACCTCCTAAGTTTGGCAGCTCAAACCCTATATATGTCATGCTGCTCGATAGATCTATTCATCAGATCTATTGGACACCTTGCCTTCAACACTGTTGGGCAACATGATCTTCAGTACAGACA
TGTCGGATCGTCATTCTCGAACTTGTCAGGATTTGAAAGGAAGTATTTCCCAGCTGATGCAGCTTCATGCAATTCCTAATTTGGGGGACTTCTTTCCCTTTCTTTAGTTTTCGGATCCTCAGGGTATCATTCGTACACCACCGC

>TRINITY_DN42503_c0_g1_i20 from sample A8 / Example unspliced isoform:

TTTCCAACGATTTTAAATTTTTATATTACATTCTATATTTAGGAAGGCGGTGAATTCCTTATCATTCTTTCAACTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGAGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTC
AGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGT
TGGCACCATCTCCAAAATAAAGTACGGAAAAGCTAATGGATGTAATCTTGGCCACTGCGAATAAGGAACCGCCGGAAAAACCTCTGGGCATAGTGAATCCGAATCACATATAATTTGATATCATTAAATATTACCGGTACCATGGATCTCTCGCCACTCCTTGCACAGAgtgagtcatttggacagtgatttacgaggta
ttttcatcgccttaccacaaatctgcgtccataggtattgaccctgtgacctgatacccttgattaaatcaatccgtgtgcagGTAAAGACAGTGTCGGAAGACCAATCGATGGCTCTCAAAGCATCTGTGCATGATGTAAGTACTGGAAATATAGTTTTCTGAAATTACAAATTACATGGCTGCCTTCAAACTATTCTA
GTAGTTGATGCAAGACGGAAAGCTGAATTCGAACCTCCTAAGTTTGGCAGCTCAAACCCTATATATGTCATGCTGCTCGATAGATCTATTCATCAGATCTATTGGACACCTTGCCTTCAACACTGTTGGGCAACATGATCTTCAGTACAGACATGTCGGATCGTCATTCTCGAACTTGTCAGGATTTGAAAGGAAGTATT
TCCCAGCTGATGCAGCTTCATGCAATTCCTAATTTGGGGGACTTCTTTCCCTTTCTTTAGTTTTCGGATCCTCAGGGTATCATTCGTACACCACCGC

Intron 5 GTAAGAGAAA ... ATCCTCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	90.16	12	110	21
A2	85.71	10	60	8
A3	84.29	11	59	19
A4	84.13	10	53	4
A5	90	4	36	8
A6	84.21	3	16	4
A7	90.79	7	69	8
A8	85.37	6	35	12
A9	69.44	11	25	6
A10	79.66	12	47	2

>TRINITY_DN42503_c0_g1_i3 from sample A8 / Example spliced isoform

AATGGAGTTGGGCGATTTCTTCTTTTCAGCATATGAAACCTGATAAGTGAGAGGAAGGCCGAGGGTGACAATGGAGTTGGGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTCCAAAATAAAGTACGGAAAAGTACCCTATTTCGATTCTTTTGC
ACCAAATCAATCTGGTGCACCAGGCGGAGGACGGCGAAATTTCCTGGCAAAGGAACCGCCG

>TRINITY_DN42503_c0_g1_i14 from sample A8 / Example unspliced isoform:

AATGGAGTTGGGCGATTTCTTCTTTTCAGCATATGAAACCTGATAAGTGAGAGGAAGGCCGAGGGTGACAATGGAGTTGGGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTCCAAAATAAAGTACGGAAAAGgtaagagaaaaaaacctgattt
ctaagatgttccttttcgaccgttgcctgagttttcaaattcaaggtcagtttatgaccagaggtttgaatagatggttctgaggcttcacccaaaatgtgatgtagaagtgttagatctattccaatagtacacagtcgttatcatggatacatagttcatttatagaagggtctctaatcctctgaggaagttaggtt
tttatcctttcagcaggttaggattctgttttgatttctttttgatagttgtgatagatcctaactgaggttaaatttagctaatcttgcttttgtttaagacaaaatatatgttaagctttcatggatacatagttcagtgatggaagggcttctaatagatttgtgatttgatttctttctaatcctcttagTACCCT

ATTTCGATTCTTTTGGACC

Intron 6 GTAAGAAAAA ... ATCCTCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	82.11	17	112	44
A2	81.82	8	60	12
A3	92.68	3	51	25
A4	87.01	5	51	16
A5	92.31	2	36	12
A6	76.47	4	16	10
A7	84.07	9	69	26
A8	76.32	9	39	19
A9	53.62	16	23	14
A10	69.41	13	51	8

>TRINITY_DN43485_c0_g1_i9 from sample A6 / Example spliced isoform

CTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGATGGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCAGGTCTCTTCATGAGGCTGTGTGGTCTGGTTTTTCAGGGTTTGAAGCGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAA
CCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTGCAAAATAAAGTACGGAAAAGTACCCTATTTCGATTCTTTTGCACCAAATCAATCTGGTG
CACCAGGCGGAGGACGGCGAAATTTCCTGGCAAAGGTATATTTCTGTGGAGATTATGATGTGATGATAGT

>TRINITY_DN43485_c0_g1_i2 from sample A6 / Example unspliced isoform:

CTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGATGGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCAGGTCTCTTCATGAGGCTGTGTGGTCTGGTTTTTCAGGGTTTGAAGCGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAA
CCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTGCAAAATAAAGTACGGAAAAGgtaagaaaaaaaacctgatttctaagatgttccttttcg
accgttgcccgagttttcaaattgaaggtcagtttatgaacagaggtttgaatagatgattctgaggcttcacccaaaatgtgatgtagaagtattagatctattccaatagtacacggccgttatcatggatacatagttcatttatagaagggtctctaattctatgaggaagttacgtttttatcctttgagcaggt
taggtttctgttttgatttattttgatacttgtgatagatcctaactgaggttaaattgagctaatcctgcttttgttcaagacaaaatatatgttaagctatcatggctacatagttcagtgatggaagggattctaatagatttgtgatttgatttctttctaatcctcttagTACCCTATTTCGATTCTTTTGCACC
AAATCAATCTGGTGCACCAGGCGGAGGACGGCGAAATTTCCTGGCAAAGGTATATTTCTGTGGAGATTATGATGTGATGATAGT

Intron 7 GTAAGGAAAA ... ATCCTCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.18	17	106	19
A2	86.67	8	52	6
A3	94.44	3	51	16
A4	90.91	5	50	4
A5	94.44	2	34	6
A6	84.21	3	16	4
A7	88.46	9	69	8
A8	80	9	36	11
A9	70	9	21	6
A10	79.69	13	51	2

>TRINITY_DN42503_c0_g1_i4 from sample A8 / Example spliced isoform

TTTCCAACGATTTTAAATTTTTATATTACATTCTATATTTAGGAAGGCGGTGAATTCCTTATCATTCTTTCAACTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGAGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTC
AGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGT
TGGCACCATCTCCAAAATAAAGTACGGAAAAGTACCCTATTTCGATTCTTTTGCACCAAATCAATCTGGTGCACCAGGCGGAGGACGGCGAAATTTCCTGGCAAAGGAACCGCCG

>TRINITY_DN42503_c0_g1_i18 from sample A8 / Example unspliced isoform:

TTTCCAACGATTTTAAATTTTTATATTACATTCTATATTTAGGAAGGCGGTGAATTCCTTATCATTCTTTCAACTCAAACGGTAGTTTCCAACGGCTCCTCGCAGGATGAGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTC
AGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTATCTTATTTTCAGCGTATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTAAGCGTATGGAACCTGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGT
TGGCACCATCTCCAAAATAAAGTACGGAAAAGgtaaggaaaaaaacctgatttctaagatgttccttttcgaccattgcccgagttttcaaattgaaggtcagtttatgaacagaggtttgaatagatgattctgaggcttcacccaaaatgtgatgtagaagtattagatctattccaatagtacacggccgttatcat
ggatacatagttcatttatagaagggtctctaattctatgaggaagttaggtttttatcctttgagcaggttaggtttctgttttgatttatgtttgatacttgtgatagatcctaactgaggttaaattgagctaatcctgcttttgttcaagacaaaatatatgttaagctatcatggctacatagttcagtgatgga
agggattctaatagatttgtgatttgatttctttctaatcctcttagTACCCTATTTCGATTCTTTTGGACC

Intron 8 GTAAGAAAAA ... GGTGCACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.83	18	109	17
A2	88.24	8	60	6
A3	81.94	13	59	5
A4	85.71	9	54	11
A5	94.74	2	36	5
A6	76.19	5	16	6
A7	94.59	4	70	15
A8	87.5	5	35	8
A9	70.27	11	26	7
A10	81.36	11	48	3

>TRINITY_DN114426_c1_g2_i11 from sample A3 / Example spliced isoform

AACGGTAGTTTCCAACGGCTCCTCGCAGGATGATGGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTCAGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTTTCTTATTTGTGGTCGTTACATGGGA
AGCCTGAGACCTGAGAAGGAGGCCGAGGGTGACAATGGAGTTGGGCGATTTCTTCTTTTCAGCATATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTGCAAAATAAA
GTACGGAAAAGGCGGAGGACGTCGAAATTGTCGTCATTGGAATCATTTACGAGCTTGGCCAGCCTCACGAT

>TRINITY_DN114426_c1_g2_i8 from sample A3 / Example unspliced isoform:

AACGGTAGTTTCCAACGGCTCCTCGCAGGATGATGGCCGTGGATTTTAAATTTTATTTTACATTTTAATTAGTATGCATGCTTTCTACTCTGGTCGCTTCATGAGGCTGTGTGGTCTGGTTTTTCAGGGTTTGAAGGGTTTCTTCTTCTCGGCTTCCCTATGCTATGTTATGTTTTCTTATTTGTGGTCGTTACATGGGA
AGCCTGAGACCTGAGAAGGAGGCCGAGGGTGACAATGGAGTTGGGCGATTTCTTCTTTTCAGCATATGAAACCTGATATGTGAGAGGAAGGTCGAGGGTGACAATGGAGTTGGGCATTTCAGCGTATGAAATCTTGCATCTGAGAGGAAGGCCGAGGGCAACAATGGAGATGGCAAGGTTGGCACCATCTGCAAAATAAA
GTACGGAAAAGgtaagaaaaaaaaacctgatttctaagatgttccttttcgaccgttgcctgagttttcaaattcaaggtcagtttatgaccagaggtttgaatagatggttctgaggcttcacccaaaatgtgatgtagaagtgttagatctattccaatagtacacagtcgttatcatggatacatagttcatttata
gaagggtctctaatcctctgaggaagttaggtttttatcctttcagcaggttaggattctgttttgatttctttttgatagttgtgatagatcctaactgaggttaaatttagctaatcttgcttttgtttaagacaaaatatatgttaagctttcatggatacatagttcagtgatggaagggcttctaatagatttgt
gatttgatttctttctaatcctcttagtaccctatttcgattcttttgcaccaaatcaatctggtgcaccagGCGGAGGACGTCGAAATTGTC