sp|P16243|MAOC_MAIZE ----> NADP-dependent malic enzyme, chloroplastic

Intron 1 GTGAGGTCTT ... TTATTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	0	2
A2	100	0	3	4
A3	55	1	1	1.5
A4	66.67	1	2	2
A5	-1	0	0	0
A6	59.82	6	8	10
A7	64.59	3	5	6
A8	100	0	1	7.5
A9	100	0	5	5
A10	69.21	3	6	7.5

>TRINITY_DN42716_c0_g1_i11 from sample A7 / Example spliced isoform

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATAATGGCATTTATTA
ATGCGGGTAAGGTCTTCTTGTCGGCCGCGGTAATGACATTTATTAACGCGGTGAGGTCTTCTCGTGGGGTTTGGCATTTACTCCCTTTCTTGATGTGTGCTTGTCATAATCAGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCT
TCAGGCCAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGTCAGGGTCCAAAGAGCTGGTTTACTTTTGGAAATGTTTTATAATCTTTCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGATATTTGACCACAACTGATCTGTCATAATTAACTG
TTCAATTCAGATCTACTCCACAATGATTTATTGTTTTAAGAATAAATGTATGCATAGACATAAGATTTCATGGATGCATAGAGAACATTCCTGCAGAACATGGAATGTATTTCTCTATCCCAGGGATGGTTGAGAGACGTCTTTTGCGTCCATCAACTGTCCCGGGGATGTTGAAAAGTCTCCAAGGCAGTTGGGAAACT
GCTCTTCTTGAACAACTTCTAATCACTTCTAGGTGCACCCTAACCATAATCAGCGGTCCTTCCTTTAGAAAAAATAACATGCCTTAAAATATTTAAAAGAAAACTGAAATTTGTCTGCCAAAATAAACTAAACAGTAAGCACAAGAGCCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGAGATCCTACTACAACTG
ATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCATGATGACTGATTGTTTTAAGAATAAATGTATCCACCTTATCATGGATGCATATATATAAGATTTCATTTCAATATGATAACTGAGTCTTTGTGAACCTTACAATTTTAGTACATTATGCTTTTGAAAAAGTGTAAGCAGAGAATCTGAGTATGCTTTCTTGT
GACAGTTTGAAGACTTCGC

>TRINITY_DN42716_c0_g1_i3 from sample A7 / Example unspliced isoform:

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATAATGGCATTTATTA
ATGCGgtgaggtcttcttgtgggttcctttgctgctgtaacagcagtgacatttactccctttcacaatagtggcattttgttacagtagtggcatttattgacagGGTAAGGTCTTCTTGTCGGCCGCGGTAATGACATTTATTAACGCGGTGAGGTCTTCTCGTGGGGTTTGGCATTTACTCCCTTTCTTGATGTGTG
CTTGTCATAATCAGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGCCAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGTCAGGGTCCAAAGAGCT
GGTTTACTTTTGGAAATGTTTTATAATCTTTCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGATATTTGACCACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCACAATGATTTATTGTTTTAAGAATAAATGTATGCATAGACATAAGATTTCATGGATGCATAGAGAACATTCCTGCAGAAC
ATGGAATGTATTTCTCTATCCCAGGGATGGTTGAGAGACGTCTTTTGCGTCCATCAACTGTCCCGGGGATGTTGAAAAGTCTCCAAGGCAGTTGGGAAACTGCTCTTCTTGAACAACTTCTAATCACTTCTAGGTGCACCCTAACCATAATCAGCGGTCCTTCCTTTAGAAAAAATAACATGCCTTAAAATATTTAAAAG
AAAACTGAAATTTGTCTGCCAAAATAAACTAAACAGTAAGCACAAGAGCCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGAGATCCTACTACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCATGATGACTGATTGTTTTAAGAATAAATGTATCCACCTTATCATGGATGCATATATATAAGA
TTTCATTTCAATATGATAACTGAGTCTTTGTGAACCTTACAATTTTAGTACATTATGCTTTTGAAAAAGTGTAAGCAGAGAATCTGAGTATGCTTTCTTGTGACAGTTTGAAGACTTCGC

Intron 2 GTGAGGTCTT ... TCATAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.24	333	0	549
A2	64.19	246	1	441
A3	62.19	391	1	643
A4	62.59	251	1	420
A5	65.21	127	0	238
A6	59.75	316	6	469
A7	61.68	707	1	1138
A8	62.69	322	2	541
A9	59.93	611	0	914
A10	62.07	658	1	1077

>TRINITY_DN42716_c0_g1_i4 from sample A7 / Example spliced isoform

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGC
CAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGCAGGGACAGGTATT

>TRINITY_DN42716_c0_g1_i11 from sample A7 / Example unspliced isoform:

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGgtgaggtcttcttggctgctgtaacttgttacagtagtggcatttattcatagggtatggtcttctggtcggtcgcgataatggcatttatta
atgcgggtaaggtcttcttgtcggccgcggtaatgacatttattaacgcggtgaggtcttctcgtggggtttggcatttactccctttcttgatgtgtgcttgtcataatcagTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCT
TCAGGCCAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGTCAGGGTCCAAAGAGCTGGTTTACTTTTGGAAATGTTTTATAATCTTTCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGATATTTGACCACAACTGATCTGTCATAATTAACTG
TTCAATTCAGATCTACTCCACAATGATTTATTGTTTTAAGAATAAATGTATGCATAGACATAAGATTTCATGGATGCATAGAGAACATTCCTGCAGAACATGGAATGTATTTCTCTATCCCAGGGATGGTTGAGAGACGTCTTTTGCGTCCATCAACTGTCCCGGGGATGTTGAAAAGTCTCCAAGGCAGTTGGGAAACT
GCTCTTCTTGAACAACTTCTAATCACTTCTAGGTGCACCCTAACCATAATCAGCGGTCCTTCCTTTAGAAAAAATAACATGCCTTAAAATATTTAAAAGAAAACTGAAATTTGTCTGCCAAAATAAACTAAACAGTAAGCACAAGAGCCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGAGATCCTACTACAACTG
ATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCATGATGACTGATTGTTTTAAGAATAAATGTATCCACCTTATCATGGATGCATATATATAAGATTTCATTTCAATATGATAACTGAGTCTTTGTGAACCTTACAATTTTAGTACATTATGCTTTTGAAAAAGTGTAAGCAGAGAATCTGAGTATGCTTTCTTGT
GACAGTTTGAAGACTTCGC

Intron 3 GTAAGGTCTT ... TCATAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0.52	192	2	0
A2	1.43	138	4	0
A3	1.03	193	2	2
A4	0.77	129	2	0
A5	0	67	0	0
A6	5.56	170	12	8
A7	1.23	443	7	4
A8	1.58	187	6	0
A9	0.6	329	4	0
A10	1.54	352	8	3

>TRINITY_DN45477_c1_g1_i1 from sample A8 / Example spliced isoform

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATAATGGCATTTATTAATGCGGTGAGGTCTTCTTGTGGGTTCCTTTGCTGCTGTAACAGCAGTGACATTTACTCCCTTTCACAATAGTGGCATTTTGTTACAGTAGTGGCATTT
ATTGACAGGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGCTAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGTCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGTCAGGGTCCAAAGAGCTGGTTT
ACTTTTGGAAATGTTTTATAATCTTTCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGATATTTGACCACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCACAATGATTTATTGTTTTAAGAATAAATGTATGCATAGACATAAGATTTCATGGATGCATAGAGAACATTCCTGCAGAACATGGA
ATGTATTTCTCTATCCCAGGGATGGTTGAGAGACGTCTTTTGCGTCCATCAACTGTCCCGGGGATGTTGAAAAGTCTCCAAGGCAGTTGGGAAACTGCTCTTCTTGAACAACTTCTAATCACTTCTGGGTGCACCCTAACCATAATCAGCGGTCCTTCCTTTAGAAAAAATAACACGCCTTAAAAATATTTAAAAGAAAA
CTGAAAATAGTCTGCCAAAATAAACTAAACAGTGAGCACAAACCCAAAGCCTAACACATGAACTAACTCGAGTTTCAATTAACTCTTGGCCTTCA

>TRINITY_DN45477_c1_g1_i5 from sample A8 / Example unspliced isoform:

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATAATGGCATTTATTAATGCGGTGAGGTCTTCTTGTGGGTTCCTTTGCTGCTGTAACAGCAGTGACATTTACTCCCTTTCACAATAGTGGCATTTTGTTACAGTAGTGGCATTT
ATTGACAGGgtaaggtcttcttgtcggccgcggtaatgacatttattaacgcggtgaggtcttctcgtggggtttggcatttactccctttctggatgtgtgcttgtcataatcagTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGA

Intron 4 GTGAGGTCTT ... TCATAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.42	333	0	553
A2	64.19	246	1	441
A3	62.11	391	1	641
A4	62.59	251	1	420
A5	65.21	127	0	238
A6	59.69	316	6	468
A7	61.68	707	1	1138
A8	62.9	322	2	546
A9	59.99	611	0	916
A10	62.05	658	1	1076

>TRINITY_DN42716_c0_g1_i4 from sample A7 / Example spliced isoform

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGC
CAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGCAGGGACAGGTATT

>TRINITY_DN42716_c0_g1_i9 from sample A7 / Example unspliced isoform:

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGgtgaggtcttcttggctgctgtaacttgttacagtagtggcatttattcatagggtatggtcttctggtcggtcgcgataatggcatttatta
atgcggtgaggtcttcttgtgggttcctttgctgctgtaacagcagtgacatttactccctttcacaatagtggcattttgttacagtagtggcatttattgacagggtaaggtcttcttgtcggccgcggtaatgacatttactaacgcggtgaggtcttctcgcggggttcttcactgctgtaacttgttacagcagt
ggcatttactccctttctggatgtgtgcttgtcataatcagTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGCCAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTC
CTTATCCACGTCAGGGTCCAAAGAGCTGGTTTACTTTTGGAAATGTTTTATAATCTTTCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGATATTTGACCACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCACAATGATTTATTGTTTTAAGAATAAATGTATGCATAGACATAAGATTTCATG
GATGCATAGAGAACATTCCTGCAGAACATGGAATGTATTTCTCTATCCCAGGGATGGTTGAGAGACGTCTTTTGCGTCCATCAACTGTCCCGGGGATGTTGAAAAGTCTCCAAGGCAGTTGGGAAACTGCTCTTCTTGAACAACTTCTAATCACTTCTAGGTGCACCCTAACCATAATCAGCGGTCCTTCCTTTAGAAAA
AATAACATGCCTTAAAATATTTAAAAGAAAACTGAAATTTGTCTGCCAAAATAAACTAAACAGTAAGCACAAGAGCCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGAGATCCTACTACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCATGATGACTGATTGTTTTAAGAATAAATGTATCCA
CCTTATCATGGATGCATATATATAAGATTTCATTTCAATATGATAACTGAGTCTTTGTGAACCTTACAATTTTAGTACATTATGCTTTTGAAAAAGTGTAAGCAGAGAATCTGAGTATGCTTTCTTGTGACAGTTTGAAGACTTCGC

Intron 5 GTGAGGTCTT ... TCATAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	62.24	333	0	549
A2	64.19	246	1	441
A3	62.19	391	1	643
A4	62.59	251	1	420
A5	65.21	127	0	238
A6	59.85	316	6	471
A7	61.68	707	1	1138
A8	62.69	322	2	541
A9	59.93	611	0	914
A10	62.07	658	1	1077

>TRINITY_DN42716_c0_g1_i4 from sample A7 / Example spliced isoform

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGC
CAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGCAGGGACAGGTATT

>TRINITY_DN42716_c0_g1_i3 from sample A7 / Example unspliced isoform:

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGgtgaggtcttcttggctgctgtaacttgttacagtagtggcatttattcatagggtatggtcttctggtcggtcgcgataatggcatttatta
atgcggtgaggtcttcttgtgggttcctttgctgctgtaacagcagtgacatttactccctttcacaatagtggcattttgttacagtagtggcatttattgacagggtaaggtcttcttgtcggccgcggtaatgacatttattaacgcggtgaggtcttctcgtggggtttggcatttactccctttcttgatgtgtg
cttgtcataatcagTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGCCAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGCCAAAAAAGCTTATGGAGAGAAAGTCCTTATCCACGTCAGGGTCCAAAGAGCT
GGTTTACTTTTGGAAATGTTTTATAATCTTTCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGATATTTGACCACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCACAATGATTTATTGTTTTAAGAATAAATGTATGCATAGACATAAGATTTCATGGATGCATAGAGAACATTCCTGCAGAAC
ATGGAATGTATTTCTCTATCCCAGGGATGGTTGAGAGACGTCTTTTGCGTCCATCAACTGTCCCGGGGATGTTGAAAAGTCTCCAAGGCAGTTGGGAAACTGCTCTTCTTGAACAACTTCTAATCACTTCTAGGTGCACCCTAACCATAATCAGCGGTCCTTCCTTTAGAAAAAATAACATGCCTTAAAATATTTAAAAG
AAAACTGAAATTTGTCTGCCAAAATAAACTAAACAGTAAGCACAAGAGCCTTGCTCCTTTTGTTGCAGAAGATGTACTGTGTTGAGATCCTACTACAACTGATCTGTCATAATTAACTGTTCAATTCAGATCTACTCCATGATGACTGATTGTTTTAAGAATAAATGTATCCACCTTATCATGGATGCATATATATAAGA
TTTCATTTCAATATGATAACTGAGTCTTTGTGAACCTTACAATTTTAGTACATTATGCTTTTGAAAAAGTGTAAGCAGAGAATCTGAGTATGCTTTCTTGTGACAGTTTGAAGACTTCGC

Intron 6 GTGAGGTCTT ... TCATAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	161	0	0
A2	1.23	120	3	0
A3	0.9	165	1	2
A4	0.48	104	1	0
A5	0	61	0	0
A6	5.75	123	7	8
A7	1.24	357	5	4
A8	0.36	137	1	0
A9	0.92	270	5	0
A10	1.45	272	5	3

>TRINITY_DN45477_c1_g1_i4 from sample A8 / Example spliced isoform

ATGGTCTTCTGGTCGGTCGCGATGATGGCATTTATTAATGCGTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGATTGAGCCAGAAGCGTGCTTCAGGCTAGGAATATGATGATATTCTCAGAGAGTTTCTGTATGCTGTCAAAAAGCTTATGGAGAGAAGTCC
TTATCCACGTCAGGGTC

>TRINITY_DN45477_c1_g1_i5 from sample A8 / Example unspliced isoform:

ATTAATGCGGTGAGGTCTTCTTGGCTGCTGTAACTTGTTACAGTAGTGGCATTTATTCATAGGGTATGGTCTTCTGGTCGGTCGCGATAATGGCATTTATTAATGCGgtgaggtcttcttgtgggttcctttgctgctgtaacagcagtgacatttactccctttcacaatagtggcattttgttacagtagtggcattt
attgacagggtaaggtcttcttgtcggccgcggtaatgacatttattaacgcggtgaggtcttctcgtggggtttggcatttactccctttctggatgtgtgcttgtcataatcagTGCCTTCAAATAACAATTGACGTGGGGACTAACAATGAAATGCTGCTAAATGATGAGTTCTACATAGGA