sp|Q9M077|AUR1_ARATH ----> Serine/threonine-protein kinase Aurora-1

Intron 1 GTGAGTACAA ... TTGTTAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.3	53	9	18
A2	24.53	40	15	11
A3	25.66	42	16	13
A4	23.61	55	12	22
A5	25.58	32	11	11
A6	21.55	284	63	93
A7	21.09	260	66	73
A8	23.24	256	73	82
A9	20.83	228	47	73
A10	25.62	180	68	56

>TRINITY_DN43004_c0_g2_i1 from sample A8 / Example spliced isoform

TACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAA
CAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAAATTCAGTGCAGCGCCT
TCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAA
TTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCT
GAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAG

>TRINITY_DN43004_c0_g2_i8 from sample A8 / Example unspliced isoform:

TGTGCATTAAAGATAAAAGAGTGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTC
CTGAACAACAGCGATGGTTGCTTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACC
TGGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAG
GGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACT
TGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTC
CCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAAgtgagtacaaattgccatcccatttcttcacctctcacatgcccacatattaacagtgattaattatgctgatgatgctgcagttgttgttaaaagATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATG
CTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGA
TTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAA
GGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGCG

Intron 2 GTTGAAACCT ... GCATTGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.76	52	20	18
A2	26.76	52	18	20
A3	23.4	72	24	20
A4	25	57	16	22
A5	30.12	29	10	15
A6	27.78	130	45	55
A7	23.48	189	50	66
A8	25.69	107	25	49
A9	28.79	188	66	86
A10	23.11	173	37	67

>TRINITY_DN45318_c0_g2_i4 from sample A10 / Example spliced isoform

CGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAACAACAGCGATGGTTGC
TTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCCAGGGAGAAGAAGAGTA
TGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGAATTGTATAAAGAGCTG
CAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTG
CGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATG
CAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATG
ATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACA
GAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATC
TTGCTAAACGCAGAGAGGGTTACTTTAAAGGGTTACTTGCTCATATCTATTTAGTGATGAAATTTAAAGGGTTACTTTTGTAAAAAAAAAA

>TRINITY_DN45318_c0_g2_i3 from sample A10 / Example unspliced isoform:

CGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAACAACAGCGATGGTTGC
TTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGgttgaaaccttctctcttcaagacaattccactcatcaattgttttgactgatgaattaaagtatttgtctctgagggattgtatgtcgggtcgggtatggcattgcaagGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGC
CATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGA
GTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTG
ATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTA
TGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCC
AAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAA
GTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACC
TAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAG

Intron 3 GTAAGTTCTT ... TTTTGCATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.38	58	0	6
A2	13.73	44	0	7
A3	18.37	40	0	9
A4	15.22	39	0	7
A5	12.5	21	0	3
A6	17.31	129	0	27
A7	17.31	172	0	36
A8	16.43	117	4	23
A9	13.02	147	0	22
A10	7.14	130	0	10

>TRINITY_DN43004_c0_g2_i9 from sample A8 / Example spliced isoform

TGTGCATTAAAGATAAAAGAGTGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTC
CTGAACAACAGCGATGGTTGCTTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACC
TGGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAG
GGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACT
TGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTC
CCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCAC
ACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATAC
AAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTA
GATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGCG

>TRINITY_DN43004_c0_g2_i3 from sample A8 / Example unspliced isoform:

TCAGTGAGAAGCGTTCAGCTACTgtaagttctttcatcatttaagttatataccttgaagggttattaagtgaatcaagtgtcttcttattctgtttgctgttttgcatagTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGG
TATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGC
AACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTT
CTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATC
GGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTG
TTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGCG

Intron 4 GTGAGTACAA ... GATGCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.79	48	9	10.6
A2	28.3	38	15	9.4
A3	29.09	39	16	8.2
A4	20.69	46	12	10.6
A5	29.73	26	11	4.1
A6	20.52	244	63	44.5
A7	21.64	239	66	42.7
A8	24.17	229	73	43.5
A9	19.34	196	47	33.7
A10	30.36	156	68	26.4

>TRINITY_DN42172_c4_g1_i1 from sample A6 / Example spliced isoform

CTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACA
TCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAAT
TCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAAT
GCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCG
ATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGA
AGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGAGGGTTACTTTAAAGGGTTACTTGCTCATATCTATTTAGTGATGAAATTTAAAGGGTTACTTTTGTATATTCTTCTAACT
GTTTTTATGATGAAAAGTTGAAGAGTAATTGTATTTAAAAAA

>TRINITY_DN42172_c4_g1_i3 from sample A6 / Example unspliced isoform:

CTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACA
TCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAAT
TCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAAgtgagtacaaattgccatcccatttcttcacctctcacatgcccacatattaacagtgattaattatgctga
tgatgctgcagTTGTTGTTAAAAGATTCAGTGCA

Intron 5 GTACTTAGTG ... TATTTTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	3.85	50	2	2
A2	3.33	58	0	2
A3	0	35	0	0
A4	4.35	44	0	2
A5	0	12	0	0
A6	3.07	158	0	5
A7	1.03	193	0	2
A8	0	123	0	0
A9	0	152	0	0
A10	0.63	158	0	1

>TRINITY_DN118068_c0_g2_i4 from sample A1 / Example spliced isoform

GTGCATTAAAGATAAAAGAGTGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCC
TGAACAACAGCGATGGTTGCTTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCT
GGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGG
GTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTT
GATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCC
CAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATTTTCGTTTGTACGTAGGCCGGCACA
CTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACA
AGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAG
ATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGAGGGTTACTTTAAAGGGTTACTTGCTCATATCTATTTAGTGATGAAATTTAAAGGGTTACTTTTATATATTAAAAA

>TRINITY_DN118068_c0_g2_i2 from sample A1 / Example unspliced isoform:

AATTTGTTGATTGGTATAAAGgtacttagtgatcatgaatttaagtttgttgttagctatttgattccagctcccaaacaacccttacagataactgatggtatattttatagGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCT
CCAGAAATGGGTGACTCATCTATGGCACTTTGCTAACCTCGTATGTGTTCTGATTTAGGTCTGC

Intron 6 GTAATCACAT ... GCATTGCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.71	52	0	18
A2	25.71	52	0	18
A3	22.58	72	1	21
A4	27.85	57	0	22
A5	34.09	29	0	15
A6	29.73	130	0	55
A7	26.74	189	1	69
A8	31.41	107	0	49
A9	31.88	188	2	88
A10	27.62	173	0	66

>TRINITY_DN46678_c0_g7_i6 from sample A7 / Example spliced isoform

ATTAAAGATAAAAGAGTGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAA
CAACAGCGATGGTTGCTTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCC
AGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGA
ATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATA
GAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAG
CCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTAT
CTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTT
CCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTG
TGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGTTACTTTTATAAAAAAA

>TRINITY_DN46678_c0_g7_i2 from sample A7 / Example unspliced isoform:

CCCAGCCAGAGCAGCAGgtaatcacatgtaaggctttcaggagagcatactaatatgcagttcaggggtcggagattctgtttagatttcagatataaggattgtatgtcgggtcgggtatggcattgcaagGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGA
AAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCA
ATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAG
CCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGT
TCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTG
GTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGA
ACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGT
TATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGTTACTTTTATAAAAAAA

Intron 7 GTGAGTACAA ... AAAGATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.59	54	9	19
A2	25	42	15	13
A3	25.66	42	16	13
A4	23.61	55	12	22
A5	25.58	32	11	11
A6	21.79	289	63	98
A7	21.28	259	66	74
A8	23.24	256	73	82
A9	20.91	227	47	73
A10	25.62	180	68	56

>TRINITY_DN46678_c0_g7_i8 from sample A7 / Example spliced isoform

ATTAAAGATAAAAGAGTGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAA
CAACAGCGATGGTTGCTTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCC
AGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGA
ATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATA
GAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAG
CCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATA
GTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAA
AGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTG
CTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGTTACTTTTATAAAAAAA

>TRINITY_DN46678_c0_g7_i5 from sample A7 / Example unspliced isoform:

ATTAAAGATAAAAGAGTGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAA
CAACAGCGATGGTTGCTTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCC
AGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGA
ATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATA
GAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAG
CCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAAgtgagtacaaattgccatcccatttcttcacctctcacatgcccacatattaacagtgattaattatgctgatgatgctgcagttgttgttaaaagattcagTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGAT
CCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCT
GATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACC
TCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGTTACTTTTATAAAAAAA

Intron 8 GTTGAAACCT ... TGTCTCTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.12	29	20	5
A2	32.5	27	18	8
A3	26.92	38	24	4
A4	32.39	24	16	7
A5	33.33	15	10	5
A6	29.47	73	45	16
A7	26.05	88	50	12
A8	27.27	40	25	5
A9	29.37	107	66	23
A10	27.68	81	37	25

>TRINITY_DN45318_c0_g2_i5 from sample A10 / Example spliced isoform

CGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAACAACAGCGATGGTTGC
TTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGGGATTGTATGTCGGGTCGGGTATGGCATTGCAAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGCCATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTT
GGCAATGTTTACCTGGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGAGTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGA
ATATGCTGCAAAGGGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTGATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGT
CTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTATGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGAT
TTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCCAAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTA
CGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAAGTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGG
TAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACCTAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTC
ATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAGGTTAATATTTAGTGATGAAATTTAAAGGGTTACTTTTGTAAAAAAAAAA

>TRINITY_DN45318_c0_g2_i3 from sample A10 / Example unspliced isoform:

CGGGAGGGAGAATTCAAACAGGTTGCGAGAAATCGCCCGGGAAGTAATAGTGCCTATTGTGATATATAAAGAATATCTATCACATGCGAGGTGCAGGTGCAACTGGTAATGATACCGTCACTAGACCTATAATCACAAAAGTTGTTCCTCCGGCCTGTATAGTCTTCTTTCCTTTGTTCCTGAACAACAGCGATGGTTGC
TTTTGTCGACGAAGAGAGGACTTCCCAGCCAGAGCAGCAGgttgaaaccttctctcttcaagacaattccactcatcaattgttttgactgatgaattaaagtatttgtctctgagGGATTGTATGTCGGGTCGGGTATGGCATTGCAAGGCTTTGGTAGCGACCAAAACGACAAAGGAGGAGGATCAGGAAAATGGTGC
CATCAAGAAGAATTATGAAAGAAAGTGGACTTTGAATGACTTTGACATCGGGAAACCTCTGGGAAGAGGCAAGTTTGGCAATGTTTACCTGGCCAGGGAGAAGAAGAGTATGTATGTTGTGGCTTTGAAAGTTCTATTCAAAAACCAACTTGAAGCATCCCAGGTAGAACATCAACTACGCAGGGAGGTTGAAATACAGA
GTCATCTTCGGCATCCCAATATACTTCGTCTGTTTGGTTATTTTTATGATAAGAGTCGTGTATATCTAGTTCTTGAATATGCTGCAAAGGGTGAATTGTATAAAGAGCTGCAACGTTATAAATTCTTCAGTGAGAAGCGTTCAGCTACTTACATTGCATCACTAGCAAGAGCTCTAATCTACTGCCATGAAAAGCATGTG
ATACATCGAGACATAAAGCCAGAGAATTTGTTGATTGGTATAAAGGGTGAACTCAAAATAGCAGACTTTGGATGGTCTGTTCATACACTTGATAGAAGGCAAACCATGTGCGGGACGTTGGATTACTTGCCTCCAGAAATGGTGGAGAACAAGGAACATGATGCTGGAGTAGACATTTGGAGCTTGGGTGTATTATTGTA
TGAATTCCTCTATGGTGTTCCTCCCTTTGAAGCAAAGCAACACTCAGATACTTATAGAAGGATTGTGAATGTTGATTTGAATTTTCCTCCCAAGCCAGTGGTTTCTAATGCAGCTAAAGATCTTATTTGTGAATTGTTGTTAAAAGATTCAGTGCAGCGCCTTCCCCTAACAAGACTTCTTCGACATCCATGGATACTCC
AAAATGCTGATCCATCTGGTGTTTATGGGGGGGAAGTTCTAGGCACATGACAAAGTGGTATCCATCTTCGTTTGTACGTAGGCCGGCACACTATCTTTTAAAACATGATGATAGTGCCATCATCCTGGCCAATATGATTCTCTTAGTCAAGTAAAAATGCAATTGCTGTCTTTCTCTACTCAAATTCGGGGGCTTTGAAA
GTCCGATTTCTGATTTGAACAGAGACTGGTTATCTATCGGAAAATTCTTCGTTTTGTTATTAGTTGTAGAGGATGGTAAAATAGGATACAAGTTCCATATTTTATCAACAGAAAGTTCAATGGCCAACTTACTATTACACTTTGAGCTCAATTCCAAAATCTGAGGTGTTGCATACAGAATAGAGTATGGTGCCGTAACC
TAGGAAGGACCTCTCTGTTATTTGTCTACCTTATTTTGTTCTCTAAAGGCATACTCGAGGGGTACACTTTCTTGTCATCTTGTACAGTAGATTGTGAGTTGGATGATATCTTGCTAAACGCAGAG