sp|Q5XET5|HESO1_ARATH ----> Protein HESO1

Intron 1 GTGAGTCTTG ... GGTCTTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	84.58	39	193	235
A2	85.56	27	128	192
A3	83.82	36	175	198
A4	86.55	32	189	223
A5	89.05	11	73	106
A6	85.79	14	80	89
A7	91.57	18	168	223
A8	80.56	28	116	116
A9	86.19	35	189	248
A10	87.5	28	168	224

>TRINITY_DN47409_c0_g1_i9 from sample A10 / Example spliced isoform

TAACAAGTTCATTTATTTTTGATCTGCTCCTCGTATTTAAAGGTCAAATCACAATTAAAGAGATCCACAAAGAGGAGGGAAAAACGAAGACTGAAGTATAAGCCCCGCCTCGAGCCAGGAGGAGAAGGTGGAGGAGCACAGTTTTACAGCGTTGTTCGAAACTTATCTGGAGTTGTTGTTCTTGACAATCATATTGGTTT
TGTGCAATGCAGGCAATGACTGGTGCTCAGAAAGGGTTTACATTTTCTCCTCCTCATGTTATACGGCGGAGAGCTGAACAACTAGAGATGGATGAAACAAAAAAATTACAGCTTGTTCCATCGTCTTTAAGTTTTCTTGGGGATCTATTTGATGAAGTCTATACCATGCTTCAACCAAATATAAAAGATTATGAAGCACG
GCAAGCATTGATAAACTTCGTTGACAAGTTTGCAAAGCTAAAGATTCGTGGAAGCAGTCTTAGCAGTTCTGGTCCCTCTGTAAGGGCATTTGGTTCGTTTACAATGGAGTTATTTACATCTGAAAGTGACTTGGATTTATCTGTGGAGGAAAGATCCGCGC

>TRINITY_DN47409_c0_g1_i5 from sample A10 / Example unspliced isoform:

TAACAAGTTCATTTATTTTTGATCTGCTCCTCGTATTTAAAGGTCAAATCACAATTAAAGAGATCCACAAAGAGGAGGGAAAAACGAAGACTGAAGTATAAGCCCCGCCTCGAGCCAGGAGGAGAAGGTGGAGGAGCACAGTTTTACAGCGTTGTTCGAAACTTATCTGGAGTTGTTGTTCTTGACAATCATATTGGTTT
TGTGCAATGCAGGCAATGACTGGTGCTCAGAAAGGGTTTACATTTTCTCCTCCTCATGTTATACGGCGGAGAGCTGAACAACTAGAGATGGATGAAACAAAAAAATTACAGCTTGTTCCATCGTCTTTAAGTTTTCTTGGGGATCTATTTGATGAAGTCTATACCATGCTTCAACCAAATATAAAAGATTATGAAGCACG
GCAAGCATTGATAAACTTCGTTGACAAGTTTGCAAAGCTAAAGATTCGTGGAAGCAGTCTTAGCAGTTCTGGTCCCTCTGTAAGGGCATTTGGTTCGTTTACAATGGAGTTATTTACATCTGAAAGTGACTTGGATTTATCTgtgagtcttggtcatactggggattctttcccaagagagaataaaattccagttttaa
agaagttggtaaaggcactttatggtcttcaagGTGGAGGAAAGATCCGTGCTGTTCAACCAGTTCTGAGGGCTATTGTGCCTGTTCTGAAATTTGTGGATTGCGGAAGTGGAATTGAGTGTGATATTTCAATTGAAAATAAGGATGGAATCTTTAAATCGGAGCTCATCCACATTTTTTCTTCAATTGACAAGAGATTT
CGGAAGCTTTGCTATCTGATGAAAGCTTGGGCAAAGGCACAAAACATCAATAGCTCGAAGGATGGAACACTAAATTCTCTTTCAATAATTCTCTTAGTTGCTTTTCACTTGCAGACTCGGGCTACTCCAATTCTTCCACCATTTTCTGTATTTTTGGAAGCTGGAGGTGGCCTTCCAGAAGTGGAGAAAAGAGTGTTGCG
CTACAAGCAGTTTGGTAATAGTAATCGAGAAACTGTTCCTGAGCTGTTCATCTCTCTCATGTGCAAGCTATTATCTGTTGAGTCTCTTTGGAAGGAAGGGCTGTGTGCAAGTACTTATGAAGGAACTTGGATTTCCAAAATGTTCAATAATAAAGTTAACTGCATGAATGTGGAGGATTTCACCGATCGTTCTCAGAATG
CTGCAAGGTCAGTAGGGAAGAGGGAGTGTGAAATAATCTACGAGTGCATAAGAGAAACTCTATCTCTTCTCCAGAAACGTTTGACAAGTTTGTCGCAGGTGTCTGATTTGAAGGCATTTCTCTTTGAACCACATTCTGGGTCAAACAAGACCTCAGGCCGGTCACCCGAGACAACAAGGCACCAGAATCTAGGAAAGAGA
AGAATGCCCTTTGAAGATATGGTTGAGCAGAAGAGGATGCGGTATTTAACAGATAAAGAGAGTGGAAACTCTTCCACTGACAAGTTAACACAAATGCAGTTTGGAGTTGAAGGTTCAAATCCAAGTGTTGCAGAAAGGATTGTCAGAACTTCAGTTCCTCCTGTACCTAGATATGAAGTTCCTCCCATGCCCGGTAATAT
TCAGTCACAAAATCAAGCATGGGGAGCAAGGCACAGTCAATGGCAAGACCCTTTGCGAGGATCAAGGATTGGCCGATCACCAGATTGGCAGCCAGGAGTACGGCATGTGCAATGGCAAGATCCAGGATATGGACCAAGGTACAGTCAGGGACATGATCCAGGATTTGGACCAAGGTACAGTCAGGGGCACGATCCAATCT
CTGGACAGACAAATTTAACTAGGAATGCTGCAGCATTTCAACCTCAATCTATCTTGCATGGGAGCTCACAATCTCATTTAATAGACCAGATGCGGATAGGGCGCTTGCTGTCCCATCTTGATCATTTAGAGCGTTATCGAGGGAAGCCTAATGCAAATATTGTGGGTTCATCTCAAAGTAACTGGAACCTTTATAATCAG
AACCATGGTAATATTCAAGGGCGTGGGAGGTATTAGGATACAGCTCGTTTTGGTAATACCTGCGTACAATGTGAAGAATGTTGGACTGCACTGTCAATGTTTGGGAAAAGAACAGTGATTTCCAAAAATTAATTTTGTAACCTTTGTTGGCATAGCTTCATTCTGAAAAAATTGCATCTTTTGTTGCCCTGTGAAGAACC
TTGAGCCTCAAGCGTAGAATGAAATAATGCCTGAGATGTAATCTTGATTGCTATTTTGTTACTATGTATGGCTGCTGAGATTTGAGCTTCTTTAGTTTGCTTTTTGTACCTGTTATAATATTTTTGATATAAAACCAATGAATTATTGCTTTGTGAAGAGACTGATAATCGATATCCATAG

Intron 2 GTCAGTAGGG ... TTTGTCGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.51	59	135.3	267
A2	79.55	60.3	176	280
A3	77.1	72.3	175.3	305
A4	74.31	63.9	114.4	250
A5	74.12	37.1	66.9	144
A6	81.2	63.6	184.7	359.6
A7	81.7	155.9	493	882.7
A8	79.65	125.4	342.1	628
A9	82.3	113	388.9	642
A10	79.88	146.4	410.1	742

>TRINITY_DN113527_c0_g1_i7 from sample A3 / Example spliced isoform

CGAAAATCGTACAAAGCATTTAGTTTCAGATGACAGCACTTCTCGTACTGTGAAAGGCTGCCATTAGAGTCAAATCGCAAAAATAAAAGAGAACAAAGAAAAATGACGGAAGAAACACTCCATTGAAGTAACGAATCACAAAAAAAAAGAATAGCAAAGTGGAGGGAAGGAGGAAGAGTGGAGCAAAAGCAGGGTCGAGG
GAGGAGGAAGAGAACATTGTTACAGGGTTGTTTGAAACTCCAGTTTTACAGGGTTGTTGGAAACTCTTACTGAAACTGTTCTCAACAATTTAATCCAGCAATGGCTAGTGCCCATAATAGGTTGACATATTCTTCATCTTATGTTATTCGGTGCAGAGCTGAACAACTGGAGATGGATGAAGCTAAAAAGTTACAGTTTG
CTTCATCATCCTTAATTTTTCTTCAGGATATATTTAATGAAGTCTATACCATGCTTCGACCAAGTATGAAAGATTATGAAGCACGACAAACATTGATTGCTTATCTTGACAAGTTTGTGAAGAAAAAGATTTGTGGAAGCAGTCTTAGCAGTTCTGATCCCTCTGTAAGGGCATTTGGTTCGTTTACAATGGACTTCTTT
ACACCGGAAAGTGACTTGGACTTATCTGTGAGTCTTGGTTATACTGGTGACACATTCCCAAGAGAAAATAAAGTGCAGGTTATCAGGAAGTTGGTAAATGCACTTTATGGTCTCCAAGGTGAAGCACAAATCTATGGTCTGGAACCTGTACTGAGGGCTATTGTGCCTGTTGTGAAATTTGTGGACTGCAGAACTGAAAT
TGAGTGTGATATTTCAATTGAAAACAAGGATGGAATCTTAAGATCAGAGCTCATCTGCATTTTCTCTTCAATTGATGAGAGATTTAAAAAGCTTTGCTATCTGATGAAAGCTTGGGCTAAGGCCCATAACATCAATAGCTCAAAAGATGGGACACTAAACTCTTTGTCAATCATTCTCTTAGTTGCTTTTCACTTGCAGA
CTCGGGCTACTCCAATTCTTCCACCATTTTCTGTATTTTTGGAAGCTGGAGGTGGCCTTCCAGAAGTGGAGAAAAGAGTGTTGCGCTACAAGCAGTTTGGTAATAGTAATCGAGAAACTGTTCCTGAGCTGTTCATCTCTCTCATGTGCAAGCTATTATCTGTTGAGTCTCTTTGGAAGGAAGGGCTGTGTGCAAGTACT
TATGAAGGAACTTGGATTTCCAAAATGTTCAATAATAAAGTTAACTGCATGAATGTGGAGGATTTCACCAATCGTTCTCAGAATGCTGCAAGGTGTCTGATTTGAAGGCATTTCTCTTTGAACCACATTCTGGGTCAAACAAGACCTCAGGCCGGTCACCCGAGACAACAAGGCACCAGAATCTAGGAAAGAGAAGAATG
CCCTTTGAAGATATGGTTGAGCAGAAGAGGATGCGGTATTTAACAGATAAAGAGAGTGGAAACTCTTCCACTGACAAGTTAACACAAATGCAGTTTGGAGTTGAAGGTTCAAATCCAAGTGTTGCAGAAAGGATTGTCAGAACTTCAGTTCCTCCTGTACCTAGATATGAAGTTCCTCCCATGCCCGGTAATATTCAGTC
ACAAAATCAAGCATGGGGAGCAAGGCACAGTCAATGGCAAGACCCTTTGCGAGGATCAAGGATTGGCCGATCACCAGATTGGCAGCCAGGAGTACGGCATGTGCAATGGCAAGATCCAGGATATGGACCAAGGTACAGTCAGGGACATGATCCAGGATTTGGACCAAGGTACAGTCAGGGGCACGATCCAATCTCTGGAC
AGACAAATTTAACTAGGAATGCTGCAGCATTTCAACCTCAATCTATCTTGCATGGGAGCTCACAATCTCATTTAATAGACCAGATGCGGATAGGGCGCTTGCTGTCCCATCTTGATCATTTAGAGCGTTATCGAGGGAAGCCTAATGCAAATATTGTGGGTTCATCTCAAAGTAACTGGAACCTTTATAATCAGAACCAT
GGTAATATTCAAGGGCGTGGGAGGTATTAGGATACAGCTCGTTTTGGTAATACCTGCGTACAATGTGAAGAATGTTGGACTGCACTGTCAATGTTTGGGAAAAGAACAGTGATTTCCAAAAATTAATTTTGTAACCTTTGTTGGCATAGCTTCATTCTGAAAAAATTGCATCTTTTGTTGCCCTGTGAAGAACCTTGAGC
CTCAAGCGTAGAATGAAATAATGCCTGAGATGTAATCTTGATTGCTATTTTGTTACTATGTATGGCTGCTGAGATTTGAGCTTCTTTAGTTTGCTTTTTGTACCTGTTATAATGAAGTATTGCTTTGTGAAGAGACTGATAATCGATATCCAT

>TRINITY_DN113527_c0_g1_i2 from sample A3 / Example unspliced isoform:

CTGGAGGTGGCCTTCCAGAAGTGGAGAAAAGAGTGTTGCGCTACAAGCAGTTTGGTAATAGTAATCGAGAAACTGTTCCTGAGCTGTTCATCTCTCTCATGTGCAAGCTATTATCTGTTGAGTCTCTTTGGAAGGAAGGGCTATGTGCAAGTACTTATGAAGGAACTTGGATTTCCAAAATGTTCAATAATAAAGTTAAC
TGCATGAATACTGATGGTAACTCCAAGACATACAGGTGGAGGATTTCACCAATCGTTCTCAGAATGCTGCAAGgtcagtagggaagagggagtgtgaaataatctacgagtgcataagagaaactctatctcttctccagaaacgtttgacaagtttgtcgcagGTGTCTGATTTGAAGGCATTTCTCTTTGAACCACAT
TCTGGGTCAAACAAGACCTCAGGCCGGTCACCCGAGACAACAAGGCACCAGAATCTAGGAAAGAGAAGAATGCCCTTTGAAGATATGGTTGAGCAGAAGAGGATGCGGTATTTAACAGATAAAGAGAGTGGAAACTCTTCCACTGACAAGTTAACACAAATGCAGTTTGGAGTTGAAGGTTCAAATCCAAGTGTTGCAGA
AAGGATTGTCAGAACTTCAGTTCCTCCTGTACCTAGATATGAAGTTCCTCCCATGCCCGGTAATATTCAGTCACAAAATCAAGCATGGGGAGCAAGGCACAGTCAATGGCAAGACCCTTTGCGAGGATCAAGGATTGGCCGATCACCAGATTGGCAGCCAGGAGTACGGCATGTGCAATGGCAAGATCCAGGATATGGAC
CAAGGTACAGTCAGGGACATGATCCAGGATTTGGACCAAGGTACAGTCAGGGGCACGATCCAATCTCTGGACAGACAAATTTAACTAGGAATGCTGCAGCATTTCAACCTCAATCTATCTTGCATGGGAGCTCACAATCTCATTTAATAGACCAGATGCGGATAGGGCGCTTGCTGTCCCATCTTGATCATTTAGAGCGT
TATCGAGGGAAGCCTAATGCAAATATTGTGGGTTCATCTCAAAGTAACTGGAACCTTTATAATCAGAACCATGGTAATATTCAAGGGCGTGGGAGGTATTAGGATACAGCTCGTTTTGGTAATACCTGCGTACAATGTGAAGAATGTTGGACTGCACTGTCAATGTTTGGGAAAAGAACAGTGATTTCCAAAAATTAATT
TTGTAACCTTTGTTGGCATAGCTTCATTCTGAAAAAATTGCATCTTTTGTTGCCCTGTGAAGAACCTTGAGCCTCAAGCGTAGAATGAAATAATGCCTGAGATGTAATCTTGATTGCTATTTTGTTACTATGTATGGCTGCTGAGATTTGAGCTTCTTTAGTTTGCTTTTTGTACCTGTTATAATGAAGTATTGCTTTGT
GAAGAGACTGATAATCGATATCCAT