sp|Q6K669|AMPL2_ORYSJ ----> Leucine aminopeptidase 2, chloroplastic

Intron 1 GTAAATTTGG ... TGCTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.08	175	2	65
A2	20.88	235	0	62
A3	20.8	217	0	57
A4	22.9	165	1	49
A5	23.16	73	3	22
A6	20.9	473	2	125
A7	22.6	839	0	245
A8	22.39	707	0	204
A9	22.94	756	0	225
A10	24.1	652	1	207

>TRINITY_DN116906_c1_g1_i2 from sample A1 / Example spliced isoform

AAACGGAAGAAAGAAAGAAGCTATGGTTTGGAGAGAGAGGATGGAATAGAGAATCTTCATTGGAGGCTTAAATATGGCATCGACTCCCCTAGCTATACCACGCACATTGGTTCTGTATTTGCCTAGATCCCCCTTTCTGTCCTTCAAATTATACCAGGAGCTGAGTCTACCTTCAAGCTTGTTACAAAGTAGTGTTAGGC
AGTTTGGATCATCTCCCAAGGTCTTATTTCAAGTGGGTTTTGCTGGACTGAGGCCTAAACTCATCTGGCAGTTGCAAAGAAGCAAGTTCACTCAAATGGGTGCAGGGGGAGTTGCTGATGTCTCATCCTTGGTTGCTGTCTCGGAGCCTAACCTTGGATTGACAAAGCCTGCAGAGGTGACTTTGCCAGAGATTACATTT
TCTGCCAAGGAAATCCACGTGACAGAGTGGAAAGGGGATATCTTGGCAGTGGGTGTGTTTGAAAAAGACGTGACAAAGGATGAGAATGGGGTACTTGAAAATCCTACGCTGAAAAAGCTTGACAGCCATGTTGGGGGTGTGTTGAGTGATGTCATGAAGGAGGAGGATTTCTCAGGGAAGGCCGGTCAGACTGCATTTGT
GAGGCTTCCGGGTTTTGGGTTTAAGAGAGTGGGATTGATTGGCCTTGGGAAGAATGGGTCTGCTTCTTTGACTAAGTCATTGAAAGGGTTTGGGGAGAATCTTGCTGGAGCTGCTAAGACTGCCCAGGCCACTGAAGTGGCTGTGACTTTCGCTTCCACCGAAGATCTTTCTATTTCTTTGAAGCTCAACGCTGTGCTGA
CTATATCAGCAGGGGTACTTCTGGGTATTTATGAGGACACTCGTTTCAAGTCAGAGGCAAAGAAGCCTTTCCTGAAGTGCTTGGATTTTGTTGGCCTCGGAAGTGGTCCTGAGTTTGAACAGAAGTTTAAGTATATCCAGCAAGTTTGTACTGGTGTTATCCTAACTAGGCAACTTGTAAATGCACCTGCAAACGTAATT
ACTCCTAGTGCGCTTGCAAATGAAGCATCCAACATTGCTTCTGCTCACAGTGATGTAATCACTGCAAAGATATTAGATGCAGAGCAATGTGAACAGTTGAAAATGGGATCATATTTAGGAGTGGCTGCAGCATCTGCAAACCCTCCAAAGTTCATTCACCTTTGCTACAAGCCTCCAACTGGAGATGTTAAGATTAAGCT
TGCCATAGTTGGAAAAGGATTGACATTTGATAGTGGAGGTTACAACATCAAGACAGGACCAGGATGTAGTATTGAACTCATGAAGTTTGACATGGGAGGTTCTGCAGCGGCTCTTGGTGCAGCAAAAGCTATTGGTCAAATTAAGCCTCCTGGGGTGGAGGTTCATTTCATTGTTGCTGCATGTGAGAACATGATTAGTG
GCACTGGTATGCGGCCTGGTGACATAGTTACAGCTTCCAATGGGAAGACAATTGAGGTAAATAACACGGATGCTGAAGGCAGGTTGACATTGGCAGATGCTTTGGTTTATGCATGTAAACAAGGCGTTGATAAGGTGGTCGATCTTGCAACTCTGACTGGAGCCTGCGTAGTTGCTCTTGGAAGTGACATTGCTGGAATG
TACACTCCCAATGACGAAATGGCAGAAGAAATATCCAAAGCTGCAGATATTGCAGGAGAAAAGTTTTGGAGAATGCCACTTGAGGAAAGCTACTGGGAATCGATGAAATCTGGAATTGCTGATATGGTGAACACTGGAGGCCGTGCAGGTGGCTCCATCACTGCTGCCCTTTTTCTTAAGCAGTTCGTGGATGAAAATGT
GCAATGGCTACATATTGACATGGCTGGACCTGTGTGGAGCGAGAAGAAACGTGGTGCAACAGGATTTGCTACCTCAACCTTGGTCGAATGGGTTTTGGAAAACTCATCATGAATACTAGCTGTGCAAATAGGGGTCTGCTGACGTTTATTATTTCAGTATTTCTGTAAACATGGTATTTTCATCCTTGCTGCCTAAGGGC
GAGAGTTAGTTTTGCCTATTTCTGGTTTTTGTCCATATGAAGCTACGTGGTAGATTCTATAGGATGCAGGACTGCTTTTTCTTTAATATCTTCTCTTTTGGCATGGATCGACAGGTTTGCTGTCTCTTTGTGTAAATTTCGTCCTTTTTGGCATTGATTTTACAGGTCAAATAAAGATGAAGATAGAAGAGTAATAAATA
TTAGATGCCAATAAATTTTTGTAAACTGGAACTGTGGCCCGAGTAATTAGACATCTGAAATATAAAATAAATTTTTAATCAAATAAAGATGAAGATAGAAG

>TRINITY_DN116906_c1_g1_i1 from sample A1 / Example unspliced isoform:

ATTAAGCCTCCTGGGGTGGAGgtaaatttggcttagatggataaattggtgttactagaaaaatatatgaacatttcagtcatggattacagaccattgccttctgatttatcacctttctgttccaaggttaaatttggaaggaagcttcgctgtacgatgataagcattgtgagttctatttgctttgtagGTTCATT
TCATTGTTGCTGCATGTGAGAACATGATTAGTGGCACTGGTATGCGGCCTGGTGACATAGTTACAGCTTCCAATGGGAAGACAATTGAGGTAAATAACACGGATGCTGAAGGCAGGTTGACATTGGCAGATGCTTTGGTTTATGCATGTAAACAAGGCGTTGATAAGGTGGTCGATCTTGCAACTCTGACTGGAGCCTGC
GTAGTTGCTCTTGGAAGTGACATTGCTGGAATGTACACTCCCAATGACGAAATGGCAGAAGAAATATCCAAAGCTGCAGATATTGCAGGAGAAAAGTTTTGGAGAATGCCACTTGAGGAAAGCTACTGGGAATCGATGAAATCTGGAATTGCTGATATGGTGAACACTGGAGGCCGTGCAGGTGGCTCCATCACTGCTGC
CCTTTTTCTTAAGCAGTTCGTGGATGAAAATGTGCAATGGCTACATATTGACATGGCTGGACCTGTGTGGAGCGAGAAGAAACGTGGTGCAACAGGATTTGCTACCTCAACCTTGGTCGAATGGGTTTTGGAAAACTCATCATGAATACTAGCTGTGCAAATAGGGGTCTGCTGACGTTTATTATTTCAGTATTTCTGTA
AACATGGTATTTTCATCCTTGCTGCCTAAGGGCGAGAGTTAGTTTTGCCTATTTCTGGTTTTTGTCCATATGAAGCTACGTGGTAGATTCTATAGGATGCAGGACTGCTTTTTCTTTAATATCTTCTCTTTTGGCATGGATCGACAGGTTTGCTGTCTCTTTGTGTAAATTTCGTCCTTTTTGGCATTGATTTTACAGGT
CAAATAAAGATGAAGATAGAAGAGTAATAAATATTAGATGCCAATAAATTTTTGTAAACTGGAACTGTGGCCCGAGTAATTAGACATCTGAAATATAAAATAAATTTTTAATCAAATAAAGATGAAGATAGAAG

Intron 2 GTATTTTCAT ... GATTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.34	43	235	116.3
A2	73.85	53.3	192	109
A3	74.01	56	231	88
A4	80.81	17.3	110	36
A5	72.45	27	98	44
A6	79.14	322	1402	1040.7
A7	74.16	454	1559	1046.7
A8	81.43	514.3	2388	2121.7
A9	78.77	392	1554	1354
A10	71.52	318.7	1082	518.3

>TRINITY_DN112088_c0_g1_i2 from sample A2 / Example spliced isoform

TATAAACGGAAGAAAGAAAGAAGCTATGGTTTGGAGAGAGAGGATGGAATAGAGAATCTTCATTGGAGGCTTAAATATGGCATCGACTCCCCTAGCCATACCACGCACATTGGCTCTGTATTTGCCTAGATCCCCCTTTCTGTCCTTCAAATTATACCAGGAGCTGAGTCTACCTTCAAGCTTGTTACAAAGTAGTGTTA
GGCAGTTTGGATCATCTCCCAAGGCCTTATTTCAAGTGGGTTTTGCTGGACTGAGGCCTAAACTCATCTGGCAGTTGCAAAGAAGCAAGTTCACTCAAATGGGTGCAGGGGGAGTTGCTGATGTCTCATCCTTGGCTGCTGTCTCGGAGCCTAACCTTGGATTGACAAAGCCTGCAGAGGTGACTTTGCCAGAGATTACA
TTTTCTGCCAAGGAAATCCACGTGACAGAGTGGAAAGGGGATATCTTGGCAGTGGGTGTGTTTGAAAAAGACGTGACAAAGGATGAGAATGGGGTACTTGAAAATCCTACGCTGAAGAAGCTTGACAGCCATGTTGGGGGTGTGTTGAGTGATGTCATGAAGGAGGAGGATTTCTCAGGGAAGGCCGGTCAGACTGCATT
TGTGAGGCTTCCGGGTTTTGGGTTTAAGAGAGTGGGATTGATTGGCCTTGGGAAGAATGGGTCTGCTTCTTTGACTAAGTCATTGAAAGGGTTTGGGGAGAATCTTGCTGGAGCTGCTAAGACTGCCCAGGCCACTGAAGTGGCTGTGACTTTCGCTTCCACCGAAGATCTTTCTATTTCTTTGAAGCTCAACGCTGTAT
TGACTATATCAGCAGGGGTACTTCTGGGTATTTACGAGGACACTCGTTTCAAGTCAGAGGCAAAGAAGCCTTTCCTGAAGTGCTTGGATTTTGTTGGCCTCGGAAGTGGTCCTGAGTTTGAACAGAAGTTTAAGTATATCCAGCAAGTTTGTACTGGTGTTATCCTAACTAGGCAACTTGTAAATGCACCTGCAAACGTA
ATTACTCCTAGTGCGCTTGCAAATGAAGCATCCAACATTGCTTCTGCTCACAGTGATGTAATCACTGCAAAGATATTAGATGCGGAGCAATGTGAACAGTTGAAAATGGGATCATATTTAGGAGTGGCTGCAGCATCTGCAAACCCTCCAAAGTTCATTCACCTTTGCTACAAGCCTCCAACTGGAGATGTTAAGATTAA
GCTTGCCATAGTTGGAAAAGGATTGACATTTGATAGTGGAGGTTACAACATCAAGACAGGACCAGGATGTAGTATTGAACTCATGAAGTTTGACATGGGAGGTTCTGCAGCGGCTCTTGGTGCAGCAAAAGCTATTGGTCAAATTAAGCCTCCTGGGGTGGAGGTTCATTTCATTGTTGCTGCATGTGAGAACATGATTA
GTGGCACTGGTATGCGGCCTGGTGACATAGTTACGGCTTCCAATGGGAAGACAATTGAGGTAAATAACACGGATGCTGAAGGCAGGTTGACATTGGCAGATGCTTTGGTTTATGCATGTAAACAAGGCGTTGATAAGGTGGTCGATCTTGCAACTCTGACTGGAGCCTGCGTAGTTGCTCTTGGAAGTGACATTGCTGGA
ATGTACACTCCCAATGACGAAATGGCAGAAGAAATATCCAAAGCTGCAGATATTGCAGGAGAAAAGTTTTGGAGAATGCCACTTGAGGAAAGCTACTGGGAATCGATGAAATCTGGAATTGCTGATATGGTGAACACTGGAGGCCGTGCAGGTGGCTCCATCACTGCTGCCCTTTTTCTTAAGCAGTTCGTGGATGAAAA
TGTGCAATGGCTACATATTGACATGGCTGGACCTGTGTGGAGCGAGAAGAAACGTGGTGCAACAGGATTTGCTACCTCAACCTTGGTCGAATGGGTTTTGGAAAACTCATCATGAATACTAGCTGTGCAAATAGGGGTCTGCTGACGTTTATTATTTCAGTATTTCTGTAAACATGGTCAAATAAAGATGAAGATAGAAG
AGTAATAAATATTAGATGCCAATAAATTTTTGTAAACTGGAACTGTAGCCCGAGTAATTAACCATCTGGAATATAAAATATTTTTTTAATCAAAGTTTATTGTTTTGGAATTTCTTCTTAATAACTTGTTAGGCAAGGTTAACTTGTTTTTATTTCGGATTGGAAGGTGAAGTAGATTTCTCAGTTCACATTTCGATAAT
G

>TRINITY_DN112088_c0_g1_i3 from sample A2 / Example unspliced isoform:

TATAAACGGAAGAAAGAAAGAAGCTATGGTTTGGAGAGAGAGGATGGAATAGAGAATCTTCATTGGAGGCTTAAATATGGCATCGACTCCCCTAGCCATACCACGCACATTGGCTCTGTATTTGCCTAGATCCCCCTTTCTGTCCTTCAAATTATACCAGGAGCTGAGTCTACCTTCAAGCTTGTTACAAAGTAGTGTTA
GGCAGTTTGGATCATCTCCCAAGGCCTTATTTCAAGTGGGTTTTGCTGGACTGAGGCCTAAACTCATCTGGCAGTTGCAAAGAAGCAAGTTCACTCAAATGGGTGCAGGGGGAGTTGCTGATGTCTCATCCTTGGCTGCTGTCTCGGAGCCTAACCTTGGATTGACAAAGCCTGCAGAGGTGACTTTGCCAGAGATTACA
TTTTCTGCCAAGGAAATCCACGTGACAGAGTGGAAAGGGGATATCTTGGCAGTGGGTGTGTTTGAAAAAGACGTGACAAAGGATGAGAATGGGGTACTTGAAAATCCTACGCTGAAGAAGCTTGACAGCCATGTTGGGGGTGTGTTGAGTGATGTCATGAAGGAGGAGGATTTCTCAGGGAAGGCCGGTCAGACTGCATT
TGTGAGGCTTCCGGGTTTTGGGTTTAAGAGAGTGGGATTGATTGGCCTTGGGAAGAATGGGTCTGCTTCTTTGACTAAGTCATTGAAAGGGTTTGGGGAGAATCTTGCTGGAGCTGCTAAGACTGCCCAGGCCACTGAAGTGGCTGTGACTTTCGCTTCCACCGAAGATCTTTCTATTTCTTTGAAGCTCAACGCTGTAT
TGACTATATCAGCAGGGGTACTTCTGGGTATTTACGAGGACACTCGTTTCAAGTCAGAGGCAAAGAAGCCTTTCCTGAAGTGCTTGGATTTTGTTGGCCTCGGAAGTGGTCCTGAGTTTGAACAGAAGTTTAAGTATATCCAGCAAGTTTGTACTGGTGTTATCCTAACTAGGCAACTTGTAAATGCACCTGCAAACGTA
ATTACTCCTAGTGCGCTTGCAAATGAAGCATCCAACATTGCTTCTGCTCACAGTGATGTAATCACTGCAAAGATATTAGATGCGGAGCAATGTGAACAGTTGAAAATGGGATCATATTTAGGAGTGGCTGCAGCATCTGCAAACCCTCCAAAGTTCATTCACCTTTGCTACAAGCCTCCAACTGGAGATGTTAAGATTAA
GCTTGCCATAGTTGGAAAAGGATTGACATTTGATAGTGGAGGTTACAACATCAAGACAGGACCAGGATGTAGTATTGAACTCATGAAGTTTGACATGGGAGGTTCTGCAGCGGCTCTTGGTGCAGCAAAAGCTATTGGTCAAATTAAGCCTCCTGGGGTGGAGGTTCATTTCATTGTTGCTGCATGTGAGAACATGATTA
GTGGCACTGGTATGCGGCCTGGTGACATAGTTACGGCTTCCAATGGGAAGACAATTGAGGTAAATAACACGGATGCTGAAGGCAGGTTGACATTGGCAGATGCTTTGGTTTATGCATGTAAACAAGGCGTTGATAAGGTGGTCGATCTTGCAACTCTGACTGGAGCCTGCGTAGTTGCTCTTGGAAGTGACATTGCTGGA
ATGTACACTCCCAATGACGAAATGGCAGAAGAAATATCCAAAGCTGCAGATATTGCAGGAGAAAAGTTTTGGAGAATGCCACTTGAGGAAAGCTACTGGGAATCGATGAAATCTGGAATTGCTGATATGGTGAACACTGGAGGCCGTGCAGGTGGCTCCATCACTGCTGCCCTTTTTCTTAAGCAGTTCGTGGATGAAAA
TGTGCAATGGCTACATATTGACATGGCTGGACCTGTGTGGAGCGAGAAGAAACGTGGTGCAACAGGATTTGCTACCTCAACCTTGGTCGAATGGGTTTTGGAAAACTCATCATGAATACTAGCTGTGCAAATAGGGGTCTGCTGACGTTTATTATTTCAGTATTTCTGTAAACATGgtattttcatccttgctgcctaag
ggcgagagttagttttgcctatttctggtttttgtccatatgaagctacgtggtagattctataggatgcaggactgctttttctttaatatcttctcttttggcatggatcgacaggtttgctgtctctttgtgtaaatttcgtcctttttggcattgattttacagGTCAAATAAAGATGAAGATAGAAGAGTAATAA
ATATTAGATGCCAATAAATTTTTGTAAACTGGAACTGTGGCCCGAGTAATTAGACATCTGAAATATAAAATAAATTTTTAATCAAAGTTTTTATTGTTTTGGAATTTCTTCT