sp|Q8LFH7|RL371_ARATH ----> Large ribosomal subunit protein eL37z

Intron 1 GTAAGTTGTT ... TGTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	10.97	120	15	14
A2	15.06	139.3	35	11
A3	12.81	165.7	28	17
A4	16.53	103	27	11
A5	16.3	86.3	22	10
A6	13.76	1958.7	395	184
A7	15.3	1413	344	136
A8	13.87	1385.7	279	141
A9	14.85	1471	351	132
A10	15.68	1167	296	112

>TRINITY_DN97515_c0_g2_i10 from sample A4 / Example spliced isoform

TTGAAAAGAACGATAAGTGGGAAGCTATTAAAAATGTCTAACACTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGAAACT
CTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGC
AAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTT
GTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTT
AAGTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAGCATTTTTCAATGACAGTA

>TRINITY_DN97515_c0_g2_i5 from sample A4 / Example unspliced isoform:

TTGAAAAGAACGATAAGTGGGAAGCTATTAAAAATGTCTAACACTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGAAACT
CTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGC
AAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGgtaagttgttaataaagcttaccaggtggttgagtaataaacctattttgtaaaagcagcacaattgctatgatgtgttattataccttgtgat
atgcatgatgtgttattataaccttgtgttgtgcagGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCA
TTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAG
CATTTTTCAATGACAGTA

Intron 2 GTGAGTCACT ... TATGTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.56	194	7	98
A2	28.36	245	13	97
A3	25.68	275	24	95
A4	20.97	147	12	39
A5	28.76	161	4	65
A6	25.18	3337.5	54	1123
A7	25.22	2512	40	847
A8	24.73	1936	41	636
A9	25.53	2374	53	814
A10	25.86	2084	31	727

>TRINITY_DN106082_c0_g1_i4 from sample A2 / Example spliced isoform

CTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGGTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACC
GTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCAT
ATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTA
AATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGT
TTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCA

>TRINITY_DN106082_c0_g1_i9 from sample A2 / Example unspliced isoform:

CTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGGTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACC
GTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGgtgagtcacttagtatccttgtccatatttcatgtgcatctcacatcttaattcatctgttattatgtggaactgtaaact
tcacttccatgattgttgcagcaggcagtagggtttattagcataggcaaatgcgtccgtgccaaaacttctatgattagagtagaaagtaggttttctatcaacacagcttccgtgccaaaacttccatgattacagtaaaaagtaggttttctatcaacacaacttccgtgccaaaacttccattacagtaaaaagta
ggttttctatcaacacaggcaatgcttcgatgccgaacgtccatggctacattatgcagtcgggcttttatcaccataggcaacggttccatacgaacacttccatgattacagtatgaagtggagtttttattagcacatgcagggattccatactggaactaccgtgattattataggtagtagccacgtatgtgcat
gtgagtttacacatcaccatacatctaatgctgctgtaaacatatgattcattaaggagaaaaaatcacagaaattaatacaaaagtgggtagggtataaggtataacgtaaactcattgagtgcacgggacttgaatgcattttcttttagttgcaatgttttatttgttcatcagttttgataagaatgtgggatggt
tggttatgttccagGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGA
ATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTT
TACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCA

Intron 3 GCAGTAGGGT ... ATTATTATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.66	190	9	88
A2	29.88	239.5	13	102
A3	24.94	268	12	89
A4	21.56	142	13	39
A5	30.47	157.5	15	69
A6	26.18	3312.5	64	1175
A7	26.29	2493	52	889
A8	26.4	1916	47	687
A9	26.74	2345.5	69	856
A10	27.17	2067	42	771

>TRINITY_DN44442_c0_g1_i4 from sample A9 / Example spliced isoform

GAAAAGAACGATAAGTGGGAAGCTATTAAAAATGTCTAACACTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGAAACTCT
TTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAA
ATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGT
GGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAA
GTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAGCATTTTTCAATGACAG

>TRINITY_DN44442_c0_g1_i8 from sample A9 / Example unspliced isoform:

GTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACCGTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGgcagtagggtttattagcataggcaaatgcgtc
cgtgccaaaacttctatgattagagtagaaagtaggttttctatcaacacagcttccgtgccaaaacttccatgattacagtaaaaagtaggttttctatcaacacaacttccgtgccaaaacttccattacagtaaaaagtaggttttctatcaacacaggcaatgcttcgatgccgaacgtccatggctacattatgc
agtcgggcttttatcaccataggcaacggttccatacgaacacttccatgattacagtatgaagtggagtttttattagcacatgcagggattccatactggaactaccgtgattattatagGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTC
CATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTC
GTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGT
TGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAGCATTTTTCAATGACAG

Intron 4 GTTATTATTT ... ATAAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	59.77	8.3	4	20.1
A2	91.61	2.4	24	26.9
A3	52.41	12.7	8	19.9
A4	66.14	6.6	7	18.6
A5	67.59	4.7	7	11.6
A6	67.41	63.4	81.6	176
A7	66.92	43.3	66.3	107.4
A8	62.27	43.8	43.6	96.6
A9	63.83	58.8	70.1	134.1
A10	64.36	39.1	57.2	81.6

>TRINITY_DN113216_c0_g2_i3 from sample A1 / Example spliced isoform

GAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACA
GGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCG
TCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGG
AAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCAC

>TRINITY_DN113216_c0_g2_i2 from sample A1 / Example unspliced isoform:

GAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGgttattattttccccttgaaacttgagaaaagaagaaaagcttggaccgtctatcgacggaccctacttagggtttgagttctta
taaatgcagAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGG
CAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGT
TGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAA
TCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCAC

Intron 5 GTGAGTTTAC ... TATGTTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	96.08	4	4	98
A2	97	3	9	97
A3	100	0	2	95
A4	100	0	4	39
A5	100	0	3	65
A6	99.91	1	2	1123
A7	99.65	3	2	847
A8	99.69	2	5	636
A9	100	0	7	814
A10	99.86	1	3	727

>TRINITY_DN106082_c0_g1_i8 from sample A2 / Example spliced isoform

CTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGGTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACC
GTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGCAGGCAGTAGGGTTTATTAGCATAGGCAAATGCGTCCGTGCCAAAACTTCTATGATTAGAGTAGAAAGTAGGTTTTCTATC
AACACAGCTTCCGTGCCAAAACTTCCATGATTACAGTAAAAAGTAGGTTTTCTATCAACACAACTTCCGTGCCAAAACTTCCATTACAGTAAAAAGTAGGTTTTCTATCAACACAGGCAATGCTTCGATGCCGAACGTCCATGGCTACATTATGCAGTCGGGCTTTTATCACCATAGGCAACGGTTCCATACGAACACTT
CCATGATTACAGTATGAAGTGGAGTTTTTATTAGCACATGCAGGGATTCCATACTGGAACTACCGTGATTATTATAGGTAGTAGCCACGTATGTGCATGGTAAGGGAACAGGAAGCCTTGG

>TRINITY_DN106082_c0_g1_i9 from sample A2 / Example unspliced isoform:

CTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGGTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACC
GTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGTGAGTCACTTAGTATCCTTGTCCATATTTCATGTGCATCTCACATCTTAATTCATCTGTTATTATGTGGAACTGTAAACT
TCACTTCCATGATTGTTGCAGCAGGCAGTAGGGTTTATTAGCATAGGCAAATGCGTCCGTGCCAAAACTTCTATGATTAGAGTAGAAAGTAGGTTTTCTATCAACACAGCTTCCGTGCCAAAACTTCCATGATTACAGTAAAAAGTAGGTTTTCTATCAACACAACTTCCGTGCCAAAACTTCCATTACAGTAAAAAGTA
GGTTTTCTATCAACACAGGCAATGCTTCGATGCCGAACGTCCATGGCTACATTATGCAGTCGGGCTTTTATCACCATAGGCAACGGTTCCATACGAACACTTCCATGATTACAGTATGAAGTGGAGTTTTTATTAGCACATGCAGGGATTCCATACTGGAACTACCGTGATTATTATAGGTAGTAGCCACGTATGTGCAT


gtgagtttacacatcaccatacatctaatgctgctgtaaacatatgattcattaaggagaaaaaatcacagaaattaatacaaaagtgggtagggtataaggtataacgtaaactcattgagtgcacgggacttgaatgcattttcttttagttgcaatgttttatttgttcatcagttttgataagaatgtgggatggt
tggttatgttccagGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGA
ATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTT
TACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCA

Intron 6 GTAGTAGCCA ... AGGGTATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.66	46	4	87
A2	75.48	51	8	106
A3	74.72	44.5	2	87
A4	75.63	19.5	4	41
A5	73.89	35	3	67
A6	75.17	588	2	1195
A7	74.63	444.5	2	863
A8	74.28	345.5	7	664
A9	74.77	429	7	848
A10	74.3	386	3	733

>TRINITY_DN45619_c0_g1_i4 from sample A8 / Example spliced isoform

GTTATTATGTGGAACTGTAAACTTCACTTCCATGATTGTTGCAGCAGGCAGTAGGGTTTATTAGCATAGGCAAATGCGTCCGTGCCAAAACTTCTATGATTAGAGTAGAAAGTAGGTTTTCTATCAACACAGCTTCCGTGCCAAAACTTCCATGATTACAGTAAAAAGTAGGTTTTCTATCAACACAACTTCCGTGCCAA
AACTTCCATTACAGTAAAAAGTAGGTTTTCTATCAACACAGGCAATGCTTCGATGCCGAACGTCCATGGCTACATTATGCAGTCGGGCTTTTATCACCATAGGCAACGGTTCCATACGAACACTTCCATGATTACAGTATGAAGTGGAGTTTTTATTAGCACATGCAGGGATTCCATACTGGAACTACCGTGATTATTAT
AGGGTAAGGGAACAGGAAGCTTT

>TRINITY_DN45619_c0_g1_i6 from sample A8 / Example unspliced isoform:

GTTATTATGTGGAACTGTAAACTTCACTTCCATGATTGTTGCAGCAGGCAGTAGGGTTTATTAGCATAGGCAAATGCGTCCGTGCCAAAACTTCTATGATTAGAGTAGAAAGTAGGTTTTCTATCAACACAGCTTCCGTGCCAAAACTTCCATGATTACAGTAAAAAGTAGGTTTTCTATCAACACAACTTCCGTGCCAA
AACTTCCATTACAGTAAAAAGTAGGTTTTCTATCAACACAGGCAATGCTTCGATGCCGAACGTCCATGGCTACATTATGCAGTCGGGCTTTTATCACCATAGGCAACGGTTCCATACGAACACTTCCATGATTACAGTATGAAGTGGAGTTTTTATTAGCACATGCAGGGATTCCATACTGGAACTACCGTGATTATTAT
AGgtagtagccacgtatgtgcatgtgagtttacacatcaccatacatctaatgctgctgtaaacatatgattcattaaggagaaaaaatcacagaaattaatacaaaagtgggtagggtataagGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTT
TCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACC
TCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAA
GTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAGCATTTTTCAATGACAGTATTTTCTTGA

Intron 7 GTGAGTCACT ... ATTGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.75	9	7	1
A2	50	13	13	5
A3	66.67	12	24	14
A4	48	13	12	4
A5	22.22	14	4	8
A6	46.15	63	54	2
A7	43.96	51	40	6
A8	47.13	46	41	1
A9	44.54	66	53	1
A10	43.06	41	31	4

>TRINITY_DN106082_c0_g1_i8 from sample A2 / Example spliced isoform

CTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGGTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACC
GTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGCAGGCAGTAGGGTTTATTAGCATAGGCAAATGCGTCCGTGCCAAAACTTCTATGATTAGAGTAGAAAGTAGGTTTTCTATC
AACACAGCTTCCGTGCCAAAACTTCCATGATTACAGTAAAAAGTAGGTTTTCTATCAACACAACTTCCGTGCCAAAACTTCCATTACAGTAAAAAGTAGGTTTTCTATCAACACAGGCAATGCTTCGATGCCGAACGTCCATGGCTACATTATGCAGTCGGGCTTTTATCACCATAGGCAACGGTTCCATACGAACACTT
CCATGATTACAGTATGAAGTGGAGTTTTTATTAGCACATGCAGGGATTCCATACTGGAACTACCGTGATTATTATAGGTAGTAGCCACGTATGTGCATGGTAAGGGAACAGGAAGCCTTGG

>TRINITY_DN106082_c0_g1_i9 from sample A2 / Example unspliced isoform:

CTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGGTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACC
GTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGgtgagtcacttagtatccttgtccatatttcatgtgcatctcacatcttaattcatctgttattatgtggaactgtaaact
tcacttccatgattgttgcagCAGGCAGTAGGGTTTATTAGCATAGGCAAATGCGTCCGTGCCAAAACTTCTATGATTAGAGTAGAAAGTAGGTTTTCTATCAACACAGCTTCCGTGCCAAAACTTCCATGATTACAGTAAAAAGTAGGTTTTCTATCAACACAACTTCCGTGCCAAAACTTCCATTACAGTAAAAAGTA
GGTTTTCTATCAACACAGGCAATGCTTCGATGCCGAACGTCCATGGCTACATTATGCAGTCGGGCTTTTATCACCATAGGCAACGGTTCCATACGAACACTTCCATGATTACAGTATGAAGTGGAGTTTTTATTAGCACATGCAGGGATTCCATACTGGAACTACCGTGATTATTATAGGTAGTAGCCACGTATGTGCAT
GTGAGTTTACACATCACCATACATCTAATGCTGCTGTAAACATATGATTCATTAAGGAGAAAAAATCACAGAAATTAATACAAAAGTGGGTAGGGTATAAGGTATAACGTAAACTCATTGAGTGCACGGGACTTGAATGCATTTTCTTTTAGTTGCAATGTTTTATTTGTTCATCAGTTTTGATAAGAATGTGGGATGGT
TGGTTATGTTCCAGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGA
ATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTT
TACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAGTGGCA

Intron 8 GCAGTAGGGT ... AGGGTATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.41	190	9	87
A2	30.69	239.5	13	106
A3	24.52	268	12	87
A4	22.42	142	13	41
A5	29.86	157.5	15	67
A6	26.51	3312.5	64	1195
A7	25.71	2493	52	863
A8	25.74	1916	47	664
A9	26.55	2345.5	69	848
A10	26.18	2067	42	733

>TRINITY_DN44442_c0_g1_i4 from sample A9 / Example spliced isoform

GAAAAGAACGATAAGTGGGAAGCTATTAAAAATGTCTAACACTTAAATCAAACCAAGCTTCCAACTTCTTTTGAAACCGAGCGAAATGTGATGTCCATCTCTTGTCCACTAAGGCAGTGCTAAAAACACCAATCCAAGAAGAGCAGGATTGTTATGCTGGGATGTTGGGTTGAACTCGCCACAAAACAATAAGAAACTCT
TTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAA
ATACAACTGGAGCGTCAAAGCTATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGT
GGGTTGCCGCCTTTTGCATTATTTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAA
GTGCTATTTAACATTGAGAGAGTGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAGCATTTTTCAATGACAG

>TRINITY_DN44442_c0_g1_i7 from sample A9 / Example unspliced isoform:

GTTATTATTTTCCCCTTGAAACTTGAGAAAAGAAGAAAAGCTTGGACCGTCTATCGACGGACCCTACTTAGGGTTTGAGTTCTTATAAATGCAGAAACTCTTTCCTTTTCTTGGCATTTGGAGGAAGGTGAAGGGAGCGAGCGTAATCCTACGCCGTCGTGACAATGgcagtagggtttattagcataggcaaatgcgtc
cgtgccaaaacttctatgattagagtagaaagtaggttttctatcaacacagcttccgtgccaaaacttccatgattacagtaaaaagtaggttttctatcaacacaacttccgtgccaaaacttccattacagtaaaaagtaggttttctatcaacacaggcaatgcttcgatgccgaacgtccatggctacattatgc
agtcgggcttttatcaccataggcaacggttccatacgaacacttccatgattacagtatgaagtggagtttttattagcacatgcagggattccatactggaactaccgtgattattataggtagtagccacgtatgtgcatgtgagtttacacatcaccatacatctaatgctgctgtaaacatatgattcattaagg
agaaaaaatcacagaaattaatacaaaagtgggtagggtataagGGTAAGGGAACAGGAAGCTTTGGTAAAAGGAGAAACAAGACACACACTCTTTGTATCCGGTGCGGTCGTCGCAGTTTCCATATCCAGAAGAGTAGATGTTCTGCATGTGCTTACCCGGCAAAGCGCATACGCAAATACAACTGGAGCGTCAAAGCT
ATTCGGAGAAAGACTACAGGGACAGGAAGAATGAGATACCTACGTGACATGCCCCGTCGTTTTAAGAACAACTTCAGGGAGGGGACGCAGGCAACTCCCAGGAAGAAGGCCTCTGCTACCTCGTAAATGTATCAAGAATGGACCTGAAATATTGAGCTGAGTTGTTAACCTCTTTTGTGGGTTGCCGCCTTTTGCATTAT
TTTGGAAATTGTTAAATATAGCTTTACTTTTACTTTTGAACTTGCATTTTTGAAGGAAATGTCTTTCCAAAACATTGATTGACGTTGTGCTGGCACTGTTTTGTGCAAAGTAATCAGAAAGTTGTTTTGAAGATTCAACAAGATGTTTACTATGTATGTTAATCATGTGTACAGTTAAGTGCTATTTAACATTGAGAGAG
TGGCTTTTAAGTTTGATATTTAATGTATGCTATGCACAAATTTTAGCATTTTTCAATGACAG