NA ----> NA

Intron 1 GTTCTATGTT ... CTGCGCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	16	20	12
A2	40.91	13	16	2
A3	61.54	10	21	11
A4	41.94	27	28	11
A5	59.26	11	20	12
A6	60.4	137	238	180
A7	58	101	155	124
A8	61.98	100	180	146
A9	57.87	91	138	112
A10	51.3	75	88	70

>TRINITY_DN97540_c0_g1_i3 from sample A4 / Example spliced isoform

TTCCATCTTATGCACATGGAATTCCAATTCCCACGTTAACTCGAGCATTAGGTGGTGGCGTTACGCTACTTAACTTGGAGAAAACACGCAAAGCTTTCCGCGCATATAACCATATTCATCGTTGTACATATTATCATATTTGGATTTGGTACCAAATAAATTTGAAACTGAACTCAATCATTCAAATACACCGAAAAATG
CATACAATTTCCTTCTGTCTTCGCCACAATCTTAAGGAAGCGTTATCACGGCATAGACAGGATACTCAGAAATTTATGCAGCCGAAGGTTCCATACGGCGCGGATTCGATTGCCAGGAAAGGAGTTTTACATGTACGACTACGGAATGCCAAAGTATATGCTCTGGGAAATAAGGATGATTCCCAGCTCGAATCGGCGGC
AAAATCAAATGAACGGGTTGGAGACAAAATGTTTAATCTTGACAGCAATGTGGATTTTACAGGTTCGAAGCAAGATTTTGGAAGTCAACCGCCGAAGGCATTAAGTCAAAATGAAGAGTTGCTGCAGAATCTCAAGAGATACGGATCATCAGGAATGCTATCATATGGATTGCTGAGTACAGTGTATTATGTTGGGACTT
TCCTATTTGTCTGATTATTGAAAATTTTGGCAATGGTTTGGGCAGGAAGTCAGGTTACTAAACTCTTGAGAGCTGGAGGGGCTCTTGCACTAGCTCCTTCTGTCGATCAAGGTTTATCTTGGTTTACTACACAGTTTAGATTCAAGTCGAGAGGAATGGCTTTTGGTGCGATTGTTGTCAGTTGTGTTACATTGGCTTTA
TCATTATTCATTGTTCTTACAATGTTATGGGCTTAAATATCTTGTCCATTTCACAAGGCCTTTGGTTATTTGTATATAGCTTTTCAAGACTTTCTTTTTCCTCAAGAATCTTACATTCCACCATGGCCAATTTACTATGCCATGAAAGTAAAATTCAATGTACAGGTGTATGTGGTAGACAATGATGATTCATAAGGAAA
AATGACACAATTATCCATGCATTGTAACCATAATGCACTTTCAATATTGCTGGC

>TRINITY_DN97540_c0_g1_i2 from sample A4 / Example unspliced isoform:

TTCCATCTTATGCACATGGAATTCCAATTCCCACGTTAACTCGAGCATTAGGTGGTGGCGTTACGCTACTTAACTTGGAGAAAACACGCAAAGCTTTCCGCGCATATAACCATATTCATCGTTGTACATATTATCATATTTGGATTTGGTACCAAATAAATTTGAAACTGAACTCAATCATTCAAATACACCGAAAAATG
CATACAATTTCCTTCTGTCTTCGCCACAATCTTAAGGAAGCGTTATCACGGCATAGACAGGATACTCAGAAATTTATGCAGCCGAAGGTTCCATACGGCGCGGATTCGATTGCCAGGAAAGGAGTTTTACATGTACGACTACGGAATGCCAAAGTATATGCTCTGGGAAATAAGGATGATTCCCAGCTCGAATCGGCGGC
AAAATCAAATGAACGGGTTGGAGACAAAATGTTTAATCTTGACAGCAATGTGGATTTTACAGGTTCGAAGCAAGATTTTGGAAGTCAACCGCCGAAGGCATTAAGTCAAAATGAAGAGTTGCTGCAGAATCTCAAGAGATACGGATCATCAGGAATGCTATCATATGGATTGCTGAGTACAGTGTATTATGTTGGGACTT
TCCTATTTGTCTGgttctatgttctccctacacctggaggtatgggctatactgctgctgcgcaaagATTATTGAAAATTTTGGCAATGGTTTGGGCAGGAAGTCAGGTTACTAAACTCTTGAGAGCTGGAGGGGCTCTTGCACTAGCTCCTTCTGTCGATCAAGGTTTATCTTGGTTTACTACACAGTTTAGATTCAAG
TCGAGAGGAATGGCTTTTGGTGCGATTGTTGTCAGTTGTGTTACATTGGCTTTATCATTATTCATTGTTCTTACAATGTTATGGGCTTAAATATCTTGTCCATTTCACAAGGCCTTTGGTTATTTGTATATAGCTTTTCAAGACTTTCTTTTTCCTCAAGAATCTTACATTCCACCATGGCCAATTTACTATGCCATGAA
AGTAAAATTCAATGTACAGGTGTATGTGGTAGACAATGATGATTCATAAGGAAAAATGACACAATTATCCATGCATTGTAACCATAATGCACTTTCAATATTGCTGGC

Intron 2 GTATGGGCTA ... CTGCGCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.66	24	17	12
A2	41.18	5	5	2
A3	46.67	16	17	11
A4	38.67	23	18	11
A5	45.45	15	13	12
A6	45.37	239	217	180
A7	45.27	159	139	124
A8	46.67	180	169	146
A9	47.18	131	122	112
A10	41.21	97	66	70

>TRINITY_DN43726_c0_g1_i2 from sample A7 / Example spliced isoform

AACTTGGAGAAAACACGCAAAGCTTTCCGCGCATATAACCATATTCATCGTTGTACATATTATCATATTTGGATTTGGTACCAAATAAATTTGAAACTGAACTCAATCATTCAAATACACCGAAAAATGCATACAATTTCCTTCTGTCTTCGCCACAATCTTAAGGAAGCGTTATCACGGCATAGACAGGATACTCAGAA
ATTTATGCAGCCGAAGGTTCCATACGGCGCGGATTCGATTGCCAGGAAAGGAGTTTTACATGTACGACTACGGAATGCCAAAGTATATGCTCTGGGAAATAAGGATGATTCCCAGCTCGAATCGGCGGCAAAATCAAATGAACGGGTTGGAGACAAAATGTTTAATCTTGACAGCAATGTGGATTTTACAGGTTCGAAGC
AAGATTTTGGAAGTCAACCGCCGAAGGCATTAAGTCAAAATGAAGAGTTGCTGCAGAATCTCAAGAGATACGGATCATCAGGAATGCTATCATATGGATTGCTGAGTACAGTGTATTATGTTGGGACTTTCCTATTTGTCTGGTTCTATGTTCTCCCTACACCTGGAGATTATTGAAAATTTTGGCAATGGTTTGGGCAG
GAAGTCAGGTTACTAAACTCTTGAGAGCTGGAGGGGCTCTTGCACTAGCTCCTTCTGTCGATCAAGGTTTATCTTGGTTTACTACACAGTTTAGATTCAAGTCGAGAGGAATGGCTTTTGGTGCGATTGTTGTCAGTTGTGTTACATTGGCTTTATCATTATTCATTGTTCTTACAATGTTATGGGCTTAAATATCTTGT
CCATTTCACAAGGCCTTTGGTTATTTGTATATAGCTTTTCAAGACTTTCTTTTTCCTCAAGAATCTTACATTCCACCATGGCCAATTTACTATGCCATGAAAGTAAAATTCAATGTACAGGTGTATGTGGTAGACAATGATGATTCATAAGGAAAAATGACACAATTATCCATGCATTGTAACCATAATGCACTTTCAAT
ATTGTTGGCCTTTTGCGTTCTTATCCTA

>TRINITY_DN43726_c0_g1_i3 from sample A7 / Example unspliced isoform:

AACTTGGAGAAAACACGCAAAGCTTTCCGCGCATATAACCATATTCATCGTTGTACATATTATCATATTTGGATTTGGTACCAAATAAATTTGAAACTGAACTCAATCATTCAAATACACCGAAAAATGCATACAATTTCCTTCTGTCTTCGCCACAATCTTAAGGAAGCGTTATCACGGCATAGACAGGATACTCAGAA
ATTTATGCAGCCGAAGGTTCCATACGGCGCGGATTCGATTGCCAGGAAAGGAGTTTTACATGTACGACTACGGAATGCCAAAGTATATGCTCTGGGAAATAAGGATGATTCCCAGCTCGAATCGGCGGCAAAATCAAATGAACGGGTTGGAGACAAAATGTTTAATCTTGACAGCAATGTGGATTTTACAGGTTCGAAGC
AAGATTTTGGAAGTCAACCGCCGAAGGCATTAAGTCAAAATGAAGAGTTGCTGCAGAATCTCAAGAGATACGGATCATCAGGAATGCTATCATATGGATTGCTGAGTACAGTGTATTATGTTGGGACTTTCCTATTTGTCTGGTTCTATGTTCTCCCTACACCTGGAGgtatgggctatactgctgctgcgcaaagATTA
TTGAAAATTTTGGCAATGGTTTGGGCAGGAAGTCAGGTTACTAAACTCTTGAGAGCTGGAGGGGCTCTTGCACTAGCTCCTTCTGTCGATCAAGGTTTATCTTGGTTTACTACACAGTTTAGATTCAAGTCGAGAGGAATGGCTTTTGGTGCGATTGTTGTCAGTTGTGTTACATTGGCTTTATCATTATTCATTGTTCT
TACAATGTTATGGGCTTAAATATCTTGTCCATTTCACAAGGCCTTTGGTTATTTGTATATAGCTTTTCAAGACTTTCTTTTTCCTCAAGAATCTTACATTCCACCATGGCCAATTTACTATGCCATGAAAGTAAAATTCAATGTACAGGTGTATGTGGTAGACAATGATGATTCATAAGGAAAAATGACACAATTATCCA
TGCATTGTAACCATAATGCACTTTCAATATTGTTGGCCTTTTGCGTTCTTATCCTA

Intron 3 GTTAGCTTGA ... CACGTGAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.06	31	9	14
A2	28	27	10	11
A3	14.29	42	9	5
A4	32.61	31	14	16
A5	24	19	5	7
A6	21.59	227	56	69
A7	21.68	215	46	73
A8	21.53	164	36	54
A9	21.44	196	51	56
A10	21.45	163	61	28

>TRINITY_DN107358_c0_g1_i3 from sample A2 / Example spliced isoform

GTTAACTCGAGCATTAGGTGGTGGCGTTACGCTACTTAACTTGGAGAAAACACGCAAAGCTTTCCGCGCATATAACCATATTCATCGTTGTACATATTATCATATTTGGATTTGGTACCAAATAAATTTGAAACTGAACTCAATCATTCAAATACACCGAAAAATGCATACAATTTCCTTCTGTCTTCGCCACAATCTTA
AGGAAGCGTTATCACGGCATAGACAGGATACTCAGAAATTTATGCAGCCGAAGGTTCCATACGGCGCGGATTCGATTGCCAGGAAAGGAGTTTTACATGTACGACTACGGAATGCCAAAGTATATGCTCTGGGAAATAAGGATGATTCCCAGCTCGAATCGGCGGCAAAATCAAATGAACGGGTTGGAGACAAAATGTTT
AATCTTGACAGCAATGTGGATTTTACAGGTTCGAAGCAAGATTTTGGAAGTCAACCGCCGAAGGCATTAAGTCAAAATGAAGAGTTGCTGCAGAATCTCAAGAGATACGGATCATCAGGAATGCTATCATATGGATTGCTGAGTACAGTGTATTATGTTGGGACTTTCCTATTTGTCTGGTTCTATGTTCTCCCTACACC
TGGAGGTATGGGCTATACTGCTGCTGCGCAAAGATTATTGAAAATTTTGGCAATGGTTTGGGCAGGAAGTCAGGTTACTAAACTCTTGAGAGCTGGAGGGGCTCTTGCACTAGCTCCTTCTGTCGATCAAGGTTTATCTTGGTTTACTACACAGTTTAGATTCAAGTCGAGAGGAATGGCTTTTGGTGCGATTGTTGTCA
GTTGTGTTACATTGGCTTTATCATTATTCATTGTTCTTACAATGTTATGGGCTTAAATATCTTGTCCATTTCACAAGGCCTTTGGTTATTTGTATATAGCTTTTCAAGACTTTCTTTTTCCTCAAGAATCTTACATTCCACCATGGCCAATTTACTATGCCATGAAAGTAAAATTCAATGTACAGGTGTATGTGGTAGAC
AATGATGATTCATAAGGAAAAATGACACAATTATCCATGCATTGTAACCATAATGCACTTTCAATATTGTTGGC

>TRINITY_DN107358_c0_g1_i2 from sample A2 / Example unspliced isoform:

GTTAACTCGAGCATTAGGTGGTGGCGTTACGCTACTTAACTTGGAGAAAACACGCAAAGCTTTCCGCGCATATAACCATATTCATCGTTGTACATATTATCATATTTGGATTTGGTACCAAATAAATTTGAAACTGAACTCAATCATTCAAATACACCGAAAAATGCATACAATTTCCTTCTGTCTTCGCCACAATCTTA
AGGAAGCGTTATCACGGCATAGACAGGATACTCAGAAATTTATGCAGCCGAAGGTTCCATACGGCGCGGATTCGATTGCCAGGAAAGGAGTTTTACATGTACGACTACGGAATGCCAAAGTATATGCTCTGGGAAATAAGGATGATTCCCAGCTCGAATCGGCGGCAAAATCAAATGAACGGgttagcttgactagctac
ggttttctgttgcaaagcgagtgcttttctctgaaattgatttggcgatgttcgaaaattcacgtgagagGTTGGAGACAAAATGTTTAATCTTGACAGCAATGTGGATTTTACAGGTTCGAAGCAAGATTTTGGAAGTCAACCGCCGAAGGCATTAAGTCAAAATGAAGAGTTGCTGCAGAATCTCAAGAGATACGGAT
CATCAGGAATGCTATCATATGGATTGCTGAGTACAGTGTATTATGTTGGGACTTTCCTATTTGTCTGGTTCTATGTTCTCCCTACACCTGGAGGTATGGGCTATACTGCTGCTGCGCAAAGATTATTGAAAATTTTGGCAATGGTTTGGGCAGGAAGTCAGGTTACTAAACTCTTGAGAGCTGGAGGGGCTCTTGCACTA
GCTCCTTCTGTCGATCAAGGTTTATCTTGGTTTACTACACAGTTTAGATTCAAGTCGAGAGGAATGGCTTTTGGTGCGATTGTTGTCAGTTGTGTTACATTGGCTTTATCATTATTCATTGTTCTTACAATGTTATGGGCTTAAATATCTTGTCCATTTCACAAGGCCTTTGGTTATTTGTATATAGCTTTTCAAGACTT
TCTTTTTCCTCAAGAATCTTACATTCCACCATGGCCAATTTACTATGCCATGAAAGTAAAATTCAATGTACAGGTGTATGTGGTAGACAATGATGATTCATAAGGAAAAATGACACAATTATCCATGCATTGTAACCATAATGCACTTTCAATATTGTTGGC