sp|Q9FQ08|KU70_ARATH ----> ATP-dependent DNA helicase 2 subunit KU70

Intron 1 GTATCATGGT ... ATGTTTTTAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	4	2	6
A2	14.29	3	0	1
A3	11.11	8	1	1
A4	-1	0	0	0
A5	-1	0	0	0
A6	36.84	6	4	3
A7	0	3	0	0
A8	33.33	9	5	4
A9	39.13	7	3	6
A10	0	1	0	0

>TRINITY_DN31635_c0_g1_i1 from sample A6 / Example spliced isoform

AAAAATCAGTGCAATCACCTTCTTCATCTCCAGGCATCTGATATGTACAGAAATCCACTGGAAAAAGAAAATCCTTTTGGACACAGCCTTAAAGCTAAAAAATCTCCAATTGGCCAGATGCCACAAGCAGCGACTTCAATATTTGAATTACAACACAATGAAACAGCTTGGAGCATTAATCTGTCCCAGTACATTGTGGC
TGTTGAAAATACTTCTGTTAAAGACTTCAGTGGACAAACTCCAGCCTGTGCAATAGTTCCAGAAAAGGGAACAAAAAGGAAAATGCCCTCTGTCACAGAAGAGGAAATTGATGTTTTCAAAGTCAAGACCAAGGAGGGAGAAATTGAGTTCTCTTTGAAGAAAGCAAGGCCAGATGAGAAGGTGGATGTTGCACTTCCAG
ATGAAACAGACATTTTAATTAATAGTCATCATGAAGTAATACCTGCTGTGGAAATTAAACATAGCAATGCCAGGAAACGGAATAGTATAGCCATTGACCTTACTGCTTTAGATGGAAAGGTGCGAGATTGTCACTCTAAGGGCAACCTTAAGTCCTTGACTGTTCCAGAACTCAAAAGCTTCCTGAGTGCAAACAAGACA
AAAGTAGGTGGCAAGAAAGAAGAGCTTATTCAACGGATTATGAGCTTGCTGTCCAATTAAAATGTCACTATTTACCATGTTGGGTTTCTCAGGTTGAATTCAGTTGATTATGTATACACAGTTCCTCAATCTTTTATGTGGCATTCCATAGTTCACTGTTTATAATATTTCACATCTCACAATTGAATTCAATTGAACTA
AATTGAAAATCTTAATACAAGAAATCCAGATTCCAGT

>TRINITY_DN31635_c0_g1_i2 from sample A6 / Example unspliced isoform:

AAAAATCAGTGCAATCACCTTCTTCATCTCCAGGCATCTGATATGTACAGAAATCCACTGGAAAAAGAAAATCCTTTTGGACACAGCCTTAAAGCTAAAAAATCTCCAATTGGCCAGATGCCACAAGCAGCGACTTCAATATTTGAATTACAACACAATGAAACAGCTTGGAGCATTAATCTGTCCCAGTACATTGTGGC
TGTTGAAAATACTTCTGTTAAAGACTTCAGTGGACAAACTCCAGCCTGTGCAATAGgtatcatggtgtggcattgattggcagttatgagttaatgacataattaaagataatgcatgtaactaattgtttgttattgaggttattatgtttttagTTCCAGAAAAGGGAACAAAAAGGAAAATGCCCTCTGTCACAGAA
GAGGAAATTGATGTTTTCAAAGTCAAGACCAAGGAGGGAGAAATTGAGTTCTCTTTGAAGAAAGCAAGGCCAGATGAGAAGGTGGATGTTGCACTTCCAGATGAAACAGACATTTTAATTAATAGTCATCATGAAGTAATACCTGCTGTGGAAATTAAACATAGCAATGCCAGGAAACGGAATAGTATAGCCATTGACCT
TACTGCTTTAGATGGAAAGGTGCGAGATTGTCACTCTAAGGGCAACCTTAAGTCCTTGACTGTTCCAGAACTCAAAAGCTTCCTGAGTGCAAACAAGACAAAAGTAGGTGGCAAGAAAGAAGAGCTTATTCAACGGATTATGAGCTTGCTGTCCAATTAAAATGTCACTATTTACCATGTTGGGTTTCTCAGGTTGAATT
CAGTTGATTATGTATACACAGTTCCTCAATCTTTTATGTGGCATTCCATAGTTCACTGTTTATAATATTTCACATCTCACAATTGAATTCAATTGAACTAAATTGAAAATCTTAATACAAGAAATCCAGATTCCAGT

Intron 2 GTTAGCTGAG ... TTGAATACAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	2	4	4
A2	73.33	2	5	6
A3	0	2	0	0
A4	0	2	0	0
A5	-1	0	0	0
A6	0	4	0	0
A7	0	2	0	0
A8	6.67	7	1	0
A9	55.56	2	3	2
A10	53.85	3	3	4

>TRINITY_DN41398_c0_g1_i2 from sample A10 / Example spliced isoform

AAAGACTTTCAAAATTTGGCAGAAGGAAACATGAATCAATATTGCATTCCTCCAATTCCCAGAGCTATGCTGATGAATACTGCTAAAATTGTACCGCACTTCATAAATCATCAGGTCGTCCATCGACAAAATGCGACTTCAGATTTTCACAGACCCAAACTTGTGTCAGCAACCATTGAACCCAAGATCAAAGACATTAA
AAATTTGGCTGAAGGAATTATGAACCAATATTGTACTTCTCCAATCTCCAGAGCTATACTGATGGATACTGCTAAAATTGTACCGCAATTTAAAAATCGTCTAGTTGTCCAGGGACAAAATGTGACTTCAGGGCACAGAATTAAAAAATCTACTAACAAGACACAGAGTGCTTACATTCTGACTAAGGACTTAGCTGCCA
TGTCAAACAAGAAAGTAGTAGAAGCAGATAGCTGCTTCTCTCCATCAATACATGCTGAAGTCAGACAGTATCCTCTCAAGGCTCCATCTTTTGCAAGGAGAAAATCTTCTACTGCAACTCTATGTAAAACAAGCAATGCTGAAAGCAGGAGCACGTCAGTTGAAAGCAGAAGCAAGAATGCAGTGAGAGGAATTACAAAT
GTCACCACAGGGTTACAGAATGGACCAAAAGTTTCAAAGAAAAAGCCAAGCGGAGCAGATAGAAAGACCGTTCCAGGTTCTCTACAAAAGAATCCAAAGAAAGCACAGGAAGGTGCTTCAACTGGAAGCAAAAAAGCTAAGGATTTATCAAAAGGACTAGCTTCTAAGAAGAAGGCATCTGATATGTACAGAAATCCACT
GGAAAAAGAAAATCCTTTTGGACACAGCCTTAAAGCTAAAAAATCTCCAATTGGCCAGATGCCACAAGCAGCGACTTCAATATTTGAATTACAACACAATGAAACAGCTTGGAGCATTAATCTGTCCCAGTACATTGTGGCTGTTGAAAATACTTCTGTTAAAGACTTCAGTGGACAAACTCCAGCCTGTGCAATAGTTC
CAGAAAAGGGAACAAAAAGGAAAATGCCCTCTGTCACAGAAGAGGAAATTGATGTTTTCAAAGTCAAGACCAAGGAGGGAGAAATTGAGTTCTCTTTGAAGAAAGCAAGGCCAGATGAGAAGGTGGATGTTGCACTTCCAGATGAAACAGACATTTTAATTAATAGTCATCATGAAGTAATACCTGCTGTGGAAATTAAA
CATAGCAATGCCAGGAAACGGAATAGTATAGCCATTGACCTTACTGCTTTAGATGGAAAGGTGCGAGATTGTCACTCTAAGGGCAACCTTAAGTCCTTGACTGTTCCAGAACTCAAAAGCTTCCTGAGTGCAAACAAGACAAAAGTAGGTGGCAAGAAAGAAGAGCTTATTCAACGGATTATGAGCTTGCTGTCCAATTA
AAATGTCACTATTTACCATGTTGGGTTTCTCAGGTTGAATTCAGTTGATTATGTATACACAGTTCCTCAATCTTTTATGTGGCATTCCATAGTTCACTGTTTATAATATTTCACATCTCACAATTGAATTCAATTGAACTAAATTGAAAATCTTAATACAAGAAATCCAG

>TRINITY_DN41398_c0_g1_i5 from sample A10 / Example unspliced isoform:

AAAGACTTTCAAAATTTGGCAGAAGGAAACATGAATCAATATTGCATTCCTCCAATTCCCAGAGCTATGCTGATGAATACTGCTAAAATTGTACCGCACTTCATAAATCATCAGGTCGTCCATCGACAAAATGCGACTTCAGATTTTCACAGACCCAAACTTGTGTCAGCAACCATTGAACCCAAGATCAAAGACATTAA
AAATTTGGCTGAAGGAATTATGAACCAATATTGTACTTCTCCAATCTCCAGAGCTATACTGATGGATACTGCTAAAATTGTACCGCAATTTAAAAATCGTCTAGTTGTCCAGGGACAAAATGTGACTTCAGGGCACAGAATTAAAAAATCTACTAACAAGACACAGAGTGCTTACATTCTGACTAAGGACTTAGCTGCCA
TGTCAAACAAGAAAGTAGTAGAAGCAGATAGCTGCTTCTCTCCATCAATACATGCTGAAGTCAGACAGTATCCTCTCAAGGCTCCATCTTTTGCAAGGAGAAAATCTTCTACTGgttagctgagtaaagatagcatttaattttagccatggctaaatttattattatgtgattagtttggcactcaacaattgattgaa
tacagCAACTCTATGTAAAACAAGCAATGCTGAAAGCAGGAGCACGTCAGTTGAAAGCAGAAGCAAGAATGCAGTGAGAGGAATTACAAATGTCACCACAGGGTTACAGAATGTAAGGGAAGAGAAAGGGTCTTCCACCAGAGTGAAAATTCTCAATCGAATATGTTTTCTTCAGTGTTTTTTCTTGCCCATCTCACTAT
GCAGTGCTTCCTGCTTTACTTCATTCACTCCTATCTCCAG

Intron 3 GTGACATATT ... AAAATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.86	2	2	1
A2	47.83	6	5	6
A3	50	7	7	7
A4	50	2	2	2
A5	-1	0	0	0
A6	33.33	1	0	1
A7	50	12	12	12
A8	44.44	10	7	9
A9	52.38	5	5	6
A10	41.18	5	3	4

>TRINITY_DN41398_c0_g1_i2 from sample A10 / Example spliced isoform

AAAGACTTTCAAAATTTGGCAGAAGGAAACATGAATCAATATTGCATTCCTCCAATTCCCAGAGCTATGCTGATGAATACTGCTAAAATTGTACCGCACTTCATAAATCATCAGGTCGTCCATCGACAAAATGCGACTTCAGATTTTCACAGACCCAAACTTGTGTCAGCAACCATTGAACCCAAGATCAAAGACATTAA
AAATTTGGCTGAAGGAATTATGAACCAATATTGTACTTCTCCAATCTCCAGAGCTATACTGATGGATACTGCTAAAATTGTACCGCAATTTAAAAATCGTCTAGTTGTCCAGGGACAAAATGTGACTTCAGGGCACAGAATTAAAAAATCTACTAACAAGACACAGAGTGCTTACATTCTGACTAAGGACTTAGCTGCCA
TGTCAAACAAGAAAGTAGTAGAAGCAGATAGCTGCTTCTCTCCATCAATACATGCTGAAGTCAGACAGTATCCTCTCAAGGCTCCATCTTTTGCAAGGAGAAAATCTTCTACTGCAACTCTATGTAAAACAAGCAATGCTGAAAGCAGGAGCACGTCAGTTGAAAGCAGAAGCAAGAATGCAGTGAGAGGAATTACAAAT
GTCACCACAGGGTTACAGAATGGACCAAAAGTTTCAAAGAAAAAGCCAAGCGGAGCAGATAGAAAGACCGTTCCAGGTTCTCTACAAAAGAATCCAAAGAAAGCACAGGAAGGTGCTTCAACTGGAAGCAAAAAAGCTAAGGATTTATCAAAAGGACTAGCTTCTAAGAAGAAGGCATCTGATATGTACAGAAATCCACT
GGAAAAAGAAAATCCTTTTGGACACAGCCTTAAAGCTAAAAAATCTCCAATTGGCCAGATGCCACAAGCAGCGACTTCAATATTTGAATTACAACACAATGAAACAGCTTGGAGCATTAATCTGTCCCAGTACATTGTGGCTGTTGAAAATACTTCTGTTAAAGACTTCAGTGGACAAACTCCAGCCTGTGCAATAGTTC
CAGAAAAGGGAACAAAAAGGAAAATGCCCTCTGTCACAGAAGAGGAAATTGATGTTTTCAAAGTCAAGACCAAGGAGGGAGAAATTGAGTTCTCTTTGAAGAAAGCAAGGCCAGATGAGAAGGTGGATGTTGCACTTCCAGATGAAACAGACATTTTAATTAATAGTCATCATGAAGTAATACCTGCTGTGGAAATTAAA
CATAGCAATGCCAGGAAACGGAATAGTATAGCCATTGACCTTACTGCTTTAGATGGAAAGGTGCGAGATTGTCACTCTAAGGGCAACCTTAAGTCCTTGACTGTTCCAGAACTCAAAAGCTTCCTGAGTGCAAACAAGACAAAAGTAGGTGGCAAGAAAGAAGAGCTTATTCAACGGATTATGAGCTTGCTGTCCAATTA
AAATGTCACTATTTACCATGTTGGGTTTCTCAGGTTGAATTCAGTTGATTATGTATACACAGTTCCTCAATCTTTTATGTGGCATTCCATAGTTCACTGTTTATAATATTTCACATCTCACAATTGAATTCAATTGAACTAAATTGAAAATCTTAATACAAGAAATCCAG

>TRINITY_DN41398_c0_g1_i3 from sample A10 / Example unspliced isoform:

AAAGACTTTCAAAATTTGGCAGAAGGAAACATGAATCAATATTGCATTCCTCCAATTCCCAGAGCTATGCTGATGAATACTGCTAAAATTGTACCGCACTTCATAAATCATCAGGTCGTCCATCGACAAAATGCGACTTCAGATTTTCACAGACCCAAACTTGTGTCAGCAACCATTGAACCCAAGATCAAAGACATTAA
AAATTTGGCTGAAGGAATTATGAACCAATATTGTACTTCTCCAATCTCCAGAGCTATACTGATGGATACTGCTAAAATTGTACCGCAATTTAAAAATCGTCTAGTTGTCCAGGGACAAAATGTGACTTCAGGGCACAGAATTAAAAAATCTACTAACAAGACACAGAGTGCTTACATTCTGACTAAGGACTTAGCTGCCA
TGTCAAACAAGAAAGTAGTAGAAGCAGATAGCTGCTTCTCTCCATCAATACATGCTGAAGTCAGACAGTATCCTCTCAAGGCTCCATCTTTTGCAAGGAGAAAATCTTCTACTGCAACTCTATGTAAAACAAGCAATGCTGAAAGCAGGAGCACGTCAGTTGAAAGCAGAAGCAAGAATGCAGTGAGAGGAATTACAAAT
GTCACCACAGGGTTACAGAATGGACCAAAAGTTTCAAAGAAAAAGCCAAGCGGAGCAGATAGAAAGACCGTTCCAGGTTCTCTACAAAAGAATCCAAAGAAAGCACAGGAAGGTGCTTCAACTGGAAGCAAAAAAGCTAAGGATTTATCAAAAGGACTAGCTTCTAAGAAGAAGgtgacatattcatctgcaaaatttca
gGCATCTGATATGTACAGAAATCCACTGGAAAAAGAAAATCCTTTTGGACACAGCCTTAAAGCTAAAAAATCTCCAATTGGCCAGATGCCACAAGCAGCGACTTCAATATTTGAATTACAACACAATGAAACAGCTTGGAGCATTAATCTGTCCCAGTACATTGTGGCTGTTGAAAATACTTCTGTTAAAGACTTCAGTG
GACAAACTCCAGCCTGTGCAATAGTTCCAGAAAAGGGAACAAAAAGGAAAATGCCCTCTGTCACAGAAGAGGAAATTGATGTTTTCAAAGTCAAGACCAAGGAGGGAGAAATTGAGTTCTCTTTGAAGAAAGCAAGGCCAGATGAGAAGGTGGATGTTGCACTTCCAGATGAAACAGACATTTTAATTAATAGTCATCAT
GAAGTAATACCTGCTGTGGAAATTAAACATAGCAATGCCAGGAAACGGAATAGTATAGCCATTGACCTTACTGCTTTAGATGGAAAGGTGCGAGATTGTCACTCTAAGGGCAACCTTAAGTCCTTGACTGTTCCAGAACTCAAAAGCTTCCTGAGTGCAAACAAGACAAAAGTAGGTGGCAAGAAAGAAGAGCTTATTCA
ACGGATTATGAGCTTGCTGTCCAATTAAAATGTCACTATTTACCATGTTGGGTTTCTCAGGTTGAATTCAGTTGATTATGTATACACAGTTCCTCAATCTTTTATGTGGCATTCCATAGTTCACTGTTTATAATATTTCACATCTCACAATTGAATTCAATTGAACTAAATTGAAAATCTTAATACAAGAAATCCAG