sp|P05621|H2B2_WHEAT ----> Histone H2B.2

Intron 1 GTTCAGTAGC ... TGCAGAAGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.38	44	279	41
A2	85.82	37	224	5
A3	87.53	45	316	18
A4	85.14	33	189	7
A5	86.75	22	144	10
A6	88.86	366	2919	284
A7	87.96	455	3323	255
A8	89.26	533	4430	478
A9	90.14	409	3739	341
A10	88.36	305	2316	190

>TRINITY_DN40388_c1_g1_i8 from sample A9 / Example spliced isoform

CATCATGAATTCTTTCCTCAATGACATCTTTGATAAACTCGCAACGGAGGCCTGCCGTCTCGCGATTTGTAACCGGAAGTCTACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTCATTTTACCCGGGGAGTTGGCAAAGCATGCGGTATCTGAGGGCAACAGGGCTGTTATTAAGTTTACTATCCCTACGTAGC
CAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAAGCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCAAGATTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGGAAAAGATTTCTTAAGAGGTTCAGTAGCCAGGCAAGATGTTTT
GAGAGCTTGAGTATATTTACGGCAAAATTCAATGATACAAATTTAAGTTCCAGATAATTAATGAAGTATTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTAAGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTG
TGTTCTGGTGGTTTTAATGATGGAAAATATTTATTAAGAGATTTGCAGAGATTTTTTGAAATTGTGTTCTGGTGG

>TRINITY_DN40388_c1_g1_i5 from sample A9 / Example unspliced isoform:

TCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTAAGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTGTGTTCTGGTGGTTTTAATGATGGAAAATATTTATTAAGAGgttcagtagcaaggcaagttattttaagagct
tgagtatatatacggtaaaattcaatgatagaaatgtaagttccaccttttcattttttacaaagatgcatatatctgatcctcagagttttcaaatatttgcagaagagATTTGCAGAGATTTTTTGAAATTGTGTTCTGGTGGTTTTAATAATGGAAAAGATTTCTTAAGAGGTTCAGTAGCTAGGCAAGATATTTTG
AAAGCTCGAATATATATAAGGCAAAATTCAATGATACAAATAGGTTGGTTGAAATTGTGTTCTGGTGGTTTTACTAATGGAAAAGATTTTTTAAAGAGGTTCAATAGCTAGGCAAGATATTTTGAGAACTTTCAGAAGGTTTTTTGAAATCATGTTCTGGTGGTTTTAATAATGTAAAGGATTTCTTAAGAGGTTCAGTA
GCTAGGCAAGATATTTTGAGAGCTTGAGTATATATACGGCAAAATTCAATGATACAAATTTTTTGAAATTGTGTTCTGGTGGTTTTAATAATGGAAAAGATTTCTTAAGAGGTTCAGTAGCTAGGCAAGATATTTTGAGAGCTTGAGTATATATACGGCAAAATTCAATG

Intron 2 GTAAATGGAC ... AATATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.11	21	17	49
A2	53.06	23	5	47
A3	61.97	27	34	54
A4	63.2	23	33	46
A5	61.17	20	17	46
A6	58.63	254	180	540
A7	61.71	179	157	420
A8	62.06	155	134	373
A9	60.68	198	171	440
A10	62.55	138	139	322

>TRINITY_DN48075_c1_g1_i4 from sample A7 / Example spliced isoform

ACTTGTCCATCCTCTAAACTGCCCATGCAACACTTGTCCATCCTCTAAACTGCCCATGCAACACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCGCTGCAGTTTTGGTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAACCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCC
CTGAATGCTCTGGTATCTTTTTGTCTGCTCAATTTTGGAGGTTCCTGGCAACTAAGAGAGGGCTAACAAAGGCAGGGACTAGCTGCTGTCGGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAAT

>TRINITY_DN48075_c1_g1_i2 from sample A7 / Example unspliced isoform:

TTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAACCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTGAATGCTCTGGTATCTTTTTGTCTGCTCAATTTTGGAGgtaaatggactttttccatgaattttccttccttgcgttttctaaatttgatggaaaatatttcagGTTCCTGGCAAC
TAAGAGAGGGCTAACAAAGGCAGGGACTAGCTGCTGTCGGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAATATCTACATATTCAAGGTCTTGAAGATGATCCATCCCGACAGCGGAATTTCTTCTAAGGCAACATCATGAATTCTTTCCTCAATGAC
ATCTTTGATCAGCTCGCAACGGAGGCCTGCAGTCTCGCGATTTGTAACCAGAAGCCCACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTTATGTTGCCAGGGGAGTTGGCCAAGCATGCGGTATCTGAGGGCAACAAGGCTGTTATTAAATTTACTAGCCCTAAGTAGCCAGCCAGCCAGCCATGGATGATGGC
CTTTTGAGTTTGATTTTTGAATCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATGCACATATCTGATCTTCAGAGTTTTCAAAGATTTCCCGAGGTTTTTTTAAATTGTGTTCTGGTTGTTTTA

Intron 3 GTTCAGTAGC ... TGAAATTAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	86.34	44	278	29
A2	85.82	37	224	2
A3	87.53	45	316	16
A4	85.14	33	189	5
A5	86.75	22	144	8
A6	88.87	366	2923	240
A7	87.95	455	3322	201
A8	89.27	533	4433	411
A9	90.14	409	3740	284
A10	88.36	305	2316	158

>TRINITY_DN40388_c1_g1_i8 from sample A9 / Example spliced isoform

CATCATGAATTCTTTCCTCAATGACATCTTTGATAAACTCGCAACGGAGGCCTGCCGTCTCGCGATTTGTAACCGGAAGTCTACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTCATTTTACCCGGGGAGTTGGCAAAGCATGCGGTATCTGAGGGCAACAGGGCTGTTATTAAGTTTACTATCCCTACGTAGC
CAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAAGCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCAAGATTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGGAAAAGATTTCTTAAGAGGTTCAGTAGCCAGGCAAGATGTTTT
GAGAGCTTGAGTATATTTACGGCAAAATTCAATGATACAAATTTAAGTTCCAGATAATTAATGAAGTATTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTAAGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTG
TGTTCTGGTGGTTTTAATGATGGAAAATATTTATTAAGAGATTTGCAGAGATTTTTTGAAATTGTGTTCTGGTGG

>TRINITY_DN40388_c1_g1_i11 from sample A9 / Example unspliced isoform:

TCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTAAGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTGTGTTCTGGTGGTTTTAATGATGGAAAATATTTATTAAGAGgttcagtagcaaggcaagttattttaagagct
tgagtatatatacggtaaaattcaatgatagaaatgtaagttccaccttttcattttttacaaagatgcatatatctgatcctcagagttttcaaatatttgcagatgtttttgaaattaagATTTGCAGAGATTTTTTGAAATTGTGTTCTGGTGGTTTTAATAATGGAAAAGATTTCTTAAGAGGTTCAGTAGCTAGG
CAAGATATTTTGAAAGCTCGAATATATATAAGGCAAAATTCAATGATACAAATAGGTTGGTTGAAATTGTGTTCTGGTGGTTTTACTAATGGAAAAGATTTTTTAAAGAGGTTCAATAGCTAGGCAAGATATTTTGAGAACTTTCAGAAGGTTTTTTGAAATCATGTTCTGGTGGTTTTAATAATGTAAAGGATTTCTTA
AGAGGTTCAGTAGCTAGGCAAGATATTTTGAGAGCTTGAGTATATATGGCGCAAAATTCAATGATACAAATGCAAGTTCCAGACAATTCATGAAGTATTTCTCACTTCTGTCTTTGGATCTTTTGAGTTTGATTTTTGAAGCGATTTCTAGTTCTTTTCATTTTTTACAAAGATGCACATATCCGATCTTCAGAATTTTC
AAAGATTTGCAGAGGGTTTTTGAAATTGTGTTCTGGTGGTTTTAATAATGGAAAAGATTTCTTAAGAGGTTCAGTAGCTAGGCAAGATATTTTGAGAGCTTGAGTATATATACGGCAAAATTCAATG

Intron 4 GTATCTTTTT ... AATATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.32	62.5	12	33.5
A2	24.01	98	2	32.5
A3	31.74	92	31	43.5
A4	36.19	66	22.5	38
A5	46.63	44	15.5	39
A6	34.95	737	141	410.5
A7	30.64	654.5	139.5	303
A8	32.51	542	109.5	273
A9	32.83	673.5	144.5	339
A10	35.01	445	112	247.5

>TRINITY_DN124914_c0_g1_i3 from sample A1 / Example spliced isoform

GAAGAAATGGACATTAATACCATGCAACACTTGTCCATCCTCTAAACTGCCCATGCAACACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCGCTGCAGTTTTGGTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAACCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTG
AATGCTCTGGTTCCTGGCAACTAAGAGAGGGCTAACAAAGGCAGGGACTAGCTGCTGTCAGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAATATCTACATATTCAAGGTCTTGAAGATGATCCATCCCGACAGCGGAATTTCTTCTAAGGCAACATC
ATGAATTCTTTCCTCAATGACATCTTTGATCAGCTCGCAACGGAGGCCTGCAGTCTCGCGATTTGTAACCAGAAGCCCACCATTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTTATGTTTCCAGGGGAGTTGGCCAAGCATGCGGTATCTGAGGGCAACAAGGCTGTTACTAACTTTACTAGCCCTAACTTAAATTT
ACTAGCCCTAAGTAGCCAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAATCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATGCACGATTTTAAAGTTTTTTTCAATTTTTACAAAGATGCATATATCTGATCTTCAGAGTTTTCAAAGATTTCCCGAGGTTTTTTGAAATTGTGTTCTGGTTGT
TTTAATGATGGAAAAGATTTCTTTAAGAGGGTCAGTAGCCAGGCAAGATATTTTGAGAGCTTGAGTATATATGGCGCAAATCTCAATGATACAAATGCAAGTTCCAGACAATTCATGAAGTATTTCTCACTTCTGTCTTTGGATCTTTTGAGTTTGATTTTTGAAGCGATTTCTAAGTTCTTTTCATTTTTTACAAAGAT
GCACATATCTGATCTTCAGAGTTTTCAAAGATTT

>TRINITY_DN124914_c0_g1_i10 from sample A1 / Example unspliced isoform:

CATGCAACACTTGTCCATCCTCTAAACTGTCAAGCTTAATTTTTCTTTGCCGCTGCAGTTTTGGTTGTATTGAAGGAAAAGTATTATCAGACCTTTGCAGATAACCATTCCACCTTCAGTTATGGCTATTGTGAAAAGAGGAACCCCTGAATGCTCTGgtatctttttgtctgctcaattttggaggtaaatggactttt
tccatgaattttccttccttgcgttttctaaatttgatggaaaatatttcagGTTCCTGGCAACTAAGAGAGGGCTAACAAAGGCAGGGACTAGCTGCTGTCAGAGATATCATCTTTGCCGTCGTCGTCAAGCGTGCCCAGGAAGAAGCGAACAAAGAAAGTGATACAGACATTCAAT

Intron 5 GTTCAGTAGC ... GTTTTCAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	90.12	44	445	358
A2	91.01	37	427	322
A3	90.63	45	494	377
A4	88.54	33	305	205
A5	91.16	22	257	197
A6	92.8	366	5717	3715
A7	91.75	455	6094	4027
A8	92.45	533	8347	4712
A9	92.86	409	6828	3804
A10	91.52	305	3982	2602

>TRINITY_DN40388_c1_g1_i8 from sample A9 / Example spliced isoform

CATCATGAATTCTTTCCTCAATGACATCTTTGATAAACTCGCAACGGAGGCCTGCCGTCTCGCGATTTGTAACCGGAAGTCTACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTCATTTTACCCGGGGAGTTGGCAAAGCATGCGGTATCTGAGGGCAACAGGGCTGTTATTAAGTTTACTATCCCTACGTAGC
CAGCCAGCCAGCCATGGATGATGGCCTTTTGAGTTTGATTTTTGAAGCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCAAGATTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGGAAAAGATTTCTTAAGAGGTTCAGTAGCCAGGCAAGATGTTTT
GAGAGCTTGAGTATATTTACGGCAAAATTCAATGATACAAATTTAAGTTCCAGATAATTAATGAAGTATTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTAAGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTG
TGTTCTGGTGGTTTTAATGATGGAAAATATTTATTAAGAGATTTGCAGAGATTTTTTGAAATTGTGTTCTGGTGG

>TRINITY_DN40388_c1_g1_i9 from sample A9 / Example unspliced isoform:

CAGCTCGCAACGGAGGCCTGTCGTCTCGCGATTTGTAACCGGAAGTCTACCGCTACTTCCAGGGAGATTCAGACTGCAGCCAGGCTCATTTTACCCGGGGAGTTGGCAAAGCATGCGGTATCTGAGGGCAACAGGGCTGTTATTAAGTTTACTATCCCTACGTAGCCAGCCAGCCAGCCATGGATGATGGCCTTTTGAGT
TTGATTTTTGAAGCGATTTTTAAGTTTTTTTCATTTTTTACAAAGATACATATATCTGATCTTCAGAGTTTTCAAAGATTTGCCAAGATTTTTTGAAATTGTGTTCTGGTTGTTTTAATGATGGAAAAGATTTCTTAAGAGGTTCAGTAGCCAGGCAAGATGTTTTGAGAGCTTGAGTATATTTACGGCAAAATTCAATG
ATACAAATTTAAGTTCTCACTTCTGTCTTTGAATCTTTTGAGTTTGCTTTTTGAAGCGATTTGTAAGTTCTTTTCATTTTCTTACAAAGTTGCATTATCTGATCTTCAGAGTTTTCAAAGATTTGCAGAGGTTTTTTGAAATTGTGTTCTGGTGGTTTTAATGATGGAAAATATTTATTAAGAGgttcagtagcaaggca
agttatttttagagcttgagaatatatacggcaaaatttaatgatagaaatgtaagttccagataattcttgaagtattcacttctgtctttgaatgttttgagtttgatttgtgaagcgagttctaagctcttttcatttttacaaagatacatatatctgatcttcagagttttcaaagATTTGCAGAGATTTTTTGA
AATTGTGTTCTGGTGGCTTTAATGATGGAAAATATTTCTTAAGAGGTTCAGTAGCCAGGCATTTTAATAATGGAAAAGATTTCTTAAGAGGTTCAGTAGCTTTTTGAAATTGTGTTCTGGTGGTTTTAATAATGGAAAAGATTTCTTAAGAGGTTCAGTAGCTAGGCAAGATATTTTGAGAGCTTGAGTATATATACGGC
AAAATTCAATG