NA ----> NA

Intron 1 GTATTTTTGT ... TCTGTACAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.27	4	3	0
A2	47.83	6	8	3
A3	28.95	19	8	7.5
A4	55.56	2	5	0
A5	75	2	11	1
A6	37.14	11	9	4
A7	52.03	7	8	7.3
A8	44.09	6	4	5.5
A9	43.86	12	16	2.8
A10	50	5	7	3

>TRINITY_DN41984_c0_g3_i4 from sample A6 / Example spliced isoform

CAAGCGTGAGATGACCGCGCTGTTAAATAACAAGGGTTTCAACACCAGTGCTGCTACTACTGAGTAATATAAGGGGTTTTCAACACCAACACTGCTACTGTTGAGTAGTATAAGGGGTTTTCAACACTAGTGTTGCTACTGCTCAATAAAAAGGGTTTCCAACACGGGTGGTGGAGGATTTTGGTGTGTAGATATACAGG
GATTCCTCAAGAAAGAATATCCAGGATTGTCAAAACAATGGAAAACTATACCTTCGTAGCATTGAATTGTGCACAGCCACCCAGCTACCCTGATGTGCTTTTCAACATATTTGCTCAAATATTGTGGAGTAACGAAATTTGAAAAGAGTGAGACCCAGAAACAAAAGAGTTGCGATGCAGAAACAAAGCATGCCATCTTG
ACTTCCAAACTTCATCTGACATAGTTATGAAAAGGATTTCGAACGACGTGGAAGGCAACGAAGATTTCACATTCAAACGCCCAACAACTGGAAGGAGGCATTTCATGTTGTTTTTTTCATGAACTGACCCAGTTCAAAGGGTCACAACACCTCTTCTAAAATTTAATGGCAGAGTTTGTCTTGAATAAAGTTCAACGATT
GACATTTGACTCTTCTTCTCCAAATGGGATTCTCCTTCTAAGCAAGTTGATTGTGGCATATGGAATGAGAGTCTTATCCATTCCAACACCTGCTTATATATATACTTATAAGTACAAGGGTATTTGGATCTTCTTGACAATTCTAACTAGGGGTGAGTTTGGAACAGTTGACTAATTTTTTTTTAGTGTTCACCATTTTA
TTGACTACTGTGTGATAAGAAAGAAATATAGTTTTTTGTGACACCAATTTTGTACAAGTGCTAGGGCATATAGATCTCTTTGACAAAGTTGTACATGTGAGAAAAATAGTTTATTGAGTTGAGGATGTGAATTTCATGATAATAGAACCTAAAGTATTAATATATCAATGACATATCTGGTTTGATC

>TRINITY_DN41984_c0_g3_i5 from sample A6 / Example unspliced isoform:

CAAGCGTGAGATGACCGCGCTGTTAAATAACAAGGGTTTCAACACCAGTGCTGCTACTACTGAGTAATATAAGGGGTTTTCAACACCAACACTGCTACTGTTGAGTAGTATAAGGGGTTTTCAACACTAGTGTTGCTACTGCTCAATAAAAAGGGTTTCCAACACGGGTGGTGGAGGATTTTGGTGTGTAGATATACAGG
GATTCCTCAAGAAAGAATATCCAGGATTGTCAAAACAATGGAAAACTATACCTTCGTAGCATTGAATTGTGCACAGCCACCCAGCTACCCTGATGTGCTTTTCAACATATgtatttttgtatccttgatcccagcattggagaatttgatttgttggattttgttgcactcttttcgggctttggtattgaatatttcca
aggaatgaataacttcttcattggatattatagaaaagataacatcttcaaatgggcatattcaagatggtgtagtcgtcaaatattagataaataccatatcagctgatatatttgtggataatctgtacaagTTGCTCAAATATTGTGGAGTAACGAAATTTGAAAAGAGTGAGACCCAGAAACAAAAGAGTTGCGAT
GCAGAAACAAAGCATGCCATCTTGACTTCCAAACTTCATCTGACATAGTTATGAAAAGGATTTCGAACGACGTGGAAGGCAACGAAGATTTCACATTCAAACGCCCAACAACTGGAAGGAGGCATTTCATGTTGTTTTTTTCATGAACTGACCCAGTTCAAAGGGTCACAACACCTCTTCTAAAATTTAATGGCAGAGTT
TGTCTTGAATAAAGTTCAACGATTGACATTTGACTCTTCTTCTCCAAATGGGATTCTCCTTCTAAGCAAGTTGATTGTGGCATATGGAATGAGAGTCTTATCCATTCCAACACCTGCTTATATATATACTTATAAGTACAAGGGTATTTGGATCTTCTTGACAATTCTAACTAGGGGTGAGTTTGGAACAGTTGACTAAT
TTTTTTTTAGTGTTCACCATTTTATTGACTACTGTGTGATAAGAAAGAAATATAGTTTTTTGTGACACCAATTTTGTACAAGTGCTAGGGCATATAGATCTCTTTGACAAAGTTGTACATGTGAGAAAAATAGTTTATTGAGTTGAGGATGTGAATTTCATGATAATAGAACCTAAAGTATTAATATATCAATGACATAT
CTGGTTTGATC

Intron 2 GTAATCTTCT ... GTTGGATCAG

Found issues: Low number of reads

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	3	0	0
A2	18.89	4.3	1	1
A3	37.64	8	2.7	7
A4	66.67	1	0	4
A5	38.89	1.7	1	1
A6	30.6	4.3	3	1
A7	16.64	7.3	2	1
A8	6.06	5	0.7	0
A9	11.11	4	0	1
A10	26.32	7	2	3

>TRINITY_DN113286_c2_g4_i2 from sample A3 / Example spliced isoform

TAATCTGTACAAGTTGCTCAAATATTGTGGAGTAACGAAATTTGAAAAGAGTGAGACCCAGAAACAAAAGAGTTGCGATGCAGAAACAAAGCATGCCATCTTGACTTCCAAACTTCATCTGACATAGTTATGAAAAGGATTTCGAACGACGTGGAAGGCAACGAAGATTTCACATTCAAACGCCCAACAACTGGAAGGAG
GCATTTCATGTTGTTTTTTTCATGAACTGACCCAGTTCAAAGGGTCACAACACCTCTTCTAAAATTTAATGGCAGAGTTTGTCTTGAATAAAGTTCAACGATTGACATTTGACTCTTCTTCTCCAAATGGGATTCTCCTTCTAAGCAAGTTGATTGTGGCATATGGAATGAGAGTCTTATCCATTCCA

>TRINITY_DN113286_c2_g4_i1 from sample A3 / Example unspliced isoform:

ATATACAGGGATTCCTCAAGAAAGAATATCCAGGATTGTCAAAACAATGGAAAACTATACCTTCGTAGCATTGAATTGTGCACAGCCACCCAGCTACCCTGATGTGCTTTTCAACATATTTGCTCAAATATTGTGGAGTAACGAAATTTGAAAAGAGTGAGACCCAGAAACAAAAGAGTTGCGATGCAGAAACAAAGCAT
GCCATCTTGACTTCCAAACTTCATCTGgtaatcttcttttagtatgcttaacccccaaaatgtttgtccgagttttataacatggtgaatattggaaaaagggtattcccctgtttgggaatgttgagaactttggatgcctgttggatcagACATAGTTATGAAAAGGATTTCGAACGACGTGGAAGGCAACGAAGATT
TCACATTCAAACGCCCAACAACTGGAAGGAGGCATTTCATGTTGTTTTTTTCATGAACTGACCCAGTTCAAAGGGTCACAACACCTCTTCTAAAATTTAATGGCAGAGTTTGTCTTGAATAAAGTTCAACGATTGACATTTGACTCTTCTTCTCCAAATGGGATTCTCCTTCTAAGCAAGTTGATTGTGGCATATGGAAT
GAGAGTCTTATCCATTCCA