NA ----> NA

Intron 1 GTCATTTTCT ... TCATTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.41	114	15	80
A2	31.46	73	11	56
A3	31.9	79	25	49
A4	29.66	51	5	38
A5	37.5	25	16	14
A6	39.37	144	103	84
A7	37.61	326	181	212
A8	37.35	354	196	226
A9	33.64	364	119	250
A10	32.3	392	129	245

>TRINITY_DN47582_c3_g2_i5 from sample A10 / Example spliced isoform

CATTATGATCACCTTGCCCAGGTATCCATTTTGTTAGATGAATGCAGCATGGCATGCAACAAAGGGATCAGCTCCTCCTCCCTTCTTCAAAGGTGTTCTATTAATTTCTTTGTATGGTGGGTTTTATTTGATTGGGGAACGAATGTCCAGGTTGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAGGCCTGGACCAAA
AGGTGCATCTGGAGATTTAGTCTTACTATGCGAGCGGTATTTGAAATGTTTGCAGATTATGCAGGTATTATGAGAGAAGAAAAACACTTTGATGTAATGTTAGATTATGATGAAGAGTTGAGAATGCTAGAAGAATGGATAGAAAATCTAAGGATTTGTGAAGACATTGATAAAAAGATTGTAGCAGATGAAGAATCTAA
AATCATTATTCCAGAATCAGAAATTAGCATTACAGAAATCCCCGAGGAAACGAAGTTAAGTAACATACACATGCAAGGAATAGAGCTGAACATTGAGATAGAAGAAATGCAAGGAATAGAGTTGAACATTGAGATAGAAGAATTATGGAAATGTTTGCAGAATACTTTTGGGTGGAGACACAATGCCACAAAGGATATAG
TTAACCACAAAGATCAGTTGATTGAAGATACTTTTGAAAGCAAGGCAAGAGGACGACAACAACATCAGATTTGGGATCCATGCGAACATCAGATTCTATATTTGAAGATGAAGATGAAAGAAATAGGCCTAAGTAGCCTAATGTGAAGCTAATTTAGTTTTATGAAATTTTTGTTAAGGGTTTGTAAGCTTGGCCAAGAC
ATAGACAGCAAGCATTTGCATTTGTGAGATTTGCATTTTAAATTTTTTAGAGTATCATAATTACAAGGGAGTGTTGTGTTTAGTAAGAATGTAACCCTTGAGATTAAGAGGGAGTGTTAGAAATAATAATCTCATTAGTTCAGTGTGTTAGTTACATTCAGTTATTCCGTTCTAAGATCGGTTAGTCGGTGTTTCCTTAA
ACCGTCAAACTATGTAACATATATATACTAATGTGTAATATGAGATAAGTAGTTCAGCCGTTCGTGCTTGTAACTCTTTTGTTTATGTCTCTATCCATATTTTGAACATGGTATCAGAGTGTTTAGTTAATTCGTTGATCAATCCAAAATGGATTTAGGAGCCTCTTCTTGATTTGAGGTTTTGTTAAACCATTAGGGCA
GAAGCCGGCCA

>TRINITY_DN47582_c3_g2_i4 from sample A10 / Example unspliced isoform:

CATTATGATCACCTTGCCCAGGTATCCATTTTGTTAGATGAATGCAGCATGGCATGCAACAAAGGGATCAGCTCCTCCTCCCTTCTTCAAAGgtcattttctttggctccctaatttttgaattctccttttctcatcggctttacttagttagggatatacataatttgaaggtgcagggatgcaaaagcagccgtcca
actacagactaccttgtcttccatttccttatagagtcattgtcttctgcacttctatcataattgtctctggtttgtcataatttcctttttcgtaatcttggtttggtttcagtgccatgtctctctgtcattttcagGTGTTCTATTAATTTCTTTGTATGGTGGGTTTTATTTGATTGGGGAACGAATGTCCAGGT
TGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAGGCCTGGACCAAAAGGTGCATCTGGAGATTTAGTCTTACTATGCGAGCGGTATTTGAAATGTTTGCAGATTATGCAGGTATTATGAGAGAAGAAAAACACTTTGATGTAATGTTAGATTATGATGAAGAGTTGAGAATGCTAGAAGAATGGATAGAAAATCTAAG
GATTTGTGAAGACATTGATAAAAAGATTGTAGCAGATGAAGAATCTAAAATCATTATTCCAGAATCAGAAATTAGCATTACAGAAATCCCCGAGGAAACGAAGTTAAGTAACATACACATGCAAGGAATAGAGCTGAACATTGAGATAGAAGAAATGCAAGGAATAGAGTTGAACATTGAGATAGAAGAATTATGGAAAT
GTTTGCAGAATACTTTTGGGTGGAGACACAATGCCACAAAGGATATAGTTAACCACAAAGATCAGTTGATTGAAGATACTTTTGAAAGCAAGGCAAGAGGACGACAACAACATCAGATTTGGGATCCATGCGAACATCAGATTCTATATTTGAAGATGAAGATGAAAGAAATAGGCCTAAGTAGCCTAATGTGAAGCTAA
TTTAGTTTTATGAAATTTTTGTTAAGGGTTTGTAAGCTTGGCCAAGACATAGACAGCAAGCATTTGCATTTGTGAGATTTGCATTTTAAATTTTTTAGAGTATCATAATTACAAGGGAGTGTTGTGTTTAGTAAGAATGTAACCCTTGAGATTAAGAGGGAGTGTTAGAAATAATAATCTCATTAGTTCAGTGTGTTAGT
TACATTCAGTTATTCCGTTCTAAGATCGGTTAGTCGGTGTTTCCTTAAACCGTCAAACTATGTAACATATATATACTAATGTGTAATATGAGATAAGTAGTTCAGCCGTTCGTGCTTGTAACTCTTTTGTTTATGTCTCTATCCATATTTTGAACATGGTATCAGAGTGTTTAGTTAATTCGTTGATCAATCCAAAATGG
ATTTAGGAGCCTCTTCTTGATTTGAGGTTTTGTTAAACCATTAGGGCAGAAGCCGGCCA

Intron 2 GTATATATCT ... ATGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.14	88	33	23
A2	9.62	94	12	8
A3	12.03	117	25	7
A4	7.45	87	7	7
A5	19.1	36	11	6
A6	12.26	204	42	15
A7	8.2	649	75	41
A8	8.3	569	77	26
A9	5.38	580	49	17
A10	8.26	600	84	24

>TRINITY_DN46016_c0_g2_i6 from sample A8 / Example spliced isoform

TGCCATGTCTCTCTGTCATTTTCAGGTGTTCTAGTAATTTTTTGTATGGTGGGTTTTATTTTATTGGGGAATGAATGTCCAGGTTGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAGGCCTGGACCAAAAGACCTGCGCATGTGGAGATTTAGTCTTACTATGCGAGCGGTAATGGAAATGTTTGCAGGTGGATCTC
CAGATTTAGCCTTACAGTATGTCGTTTAATTTTAATGCAGCCATTGATTAGAATTCATGTGTACTTAAACTAGAGGAGTTTCGAGGTGGAAACATA

>TRINITY_DN46016_c0_g2_i8 from sample A8 / Example unspliced isoform:

TGCCATGTCTCTCTGTCATTTTCAGGTGTTCTAGTAATTTTTTGTATGGTGGGTTTTATTTTATTGGGGAATGAATGTCCAGGTTGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAGGCCTGGACCAAAAGgtatatatctttctatttaaaacttttttattcatttttaaaaagtggtgtactaggctttactca
tctttgataatgtgaaatgtttgcagACCTGCGCATGTGGAGATTTAGTCTTACTATGCGAGCGGTAATGGAAATGTTTGCAGGTGGATCTCCAGATTTAGCCTTACAGTATGTCGTTTAATTTTAATGCAGCCATTGATTAGAATTCATGTGTACTTAAACTAGAGG

Intron 3 GTATATATCT ... ATGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	24.64	170	54.5	22.3
A2	15.65	145.2	26.4	13
A3	19.51	166	38.3	17.4
A4	11.91	119.4	15.2	13
A5	27.2	49.5	17.4	6.5
A6	15.48	302.3	53.4	13.4
A7	9.98	1142.5	121	48.2
A8	11.56	978	122.6	36
A9	11.83	906.8	117.9	20.7
A10	13.15	976.4	141.3	29

>TRINITY_DN103823_c5_g2_i16 from sample A4 / Example spliced isoform

ATGTAACACTTTATTTTGCAATAGGTTGTACAGGTATCGATTTCGTTAGATGAATGCAGCATGGCATGCAACAAAGGGATCAGCTCCTCCTCCCTTCTTCAAAGGTGTTCTATTAATTTCTTTGTATGGTGGGTTTTATTTGATTGGGGAACGAATGTCCAGGTTGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAG
GCCTGGACCAAAAGGTGCATCTGGAGATTTATTCTTACTATGCGAGCGGTAATTGAAATGTTTGCAGATTATGCAGGTATTATGAGAGAAGAAAAACACTTTGATGTAATGTTAGATTATGATGAAGAGTTGAGAATGCTAGAAGAATGGATAGAAAATCTAAGGATTTGTGAAGACATTGATAAAAAGATTGTAGCAGA
TGAAGAATCTAAAATCATTATTCCAGAATCAGAAATTAGCATTACAGAAATCCCCGAGGAAACGAAGTTAAGTAACATACACATGCAAGGAATAGAGCTGAACATTGAGATAGAAGAAATGCAAGGAATAGAGTTGAACATTGAGATAGAAGAATTATGGAAATGTTTGCAGAATACTTTTGGGTGGAGACACAATGCCA
CAAAGGATATAGTTAACCACAAAGATCAGTTGATTGAAGATACTTTTGAAAGCAAGGCAAGAGGACGACAACAACATCAGATTTGGGATCCATGCGAACATCAGATTCTATATTTGAAGATGAAGATGAAAGAAATAGGCCTAAGCAGCCTAATCAAGAAATCCAGCTTATTTTATTTTCTCAGTATTGTAAGCCTAGCT
GAGACATAGCCCAGCAGGCATTTGCATTTCTGAAATTTGCATTTTAGCAGTATTAGAGTATTATAGTATCATAATTAAGAGGGAGT

>TRINITY_DN103823_c5_g2_i10 from sample A4 / Example unspliced isoform:

TGCCATGTCTCTCTGTCATTTTCAGGTGTTCTATTAATTTCTTTGTATGGTGGGTTTTATTTGATTGGGGAACGAATGTCCAGGTTGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAGGCCTGGACCAAAAGgtatatatctttctatttaaaccttttttattcaactttaaaactaggtgtaccgcgctttactc
atctttgataatgtgaaatgtttgcagGTGCATCTGGAGATTTATTCTTACTATGCGAGCGGTAATTGAAATGTTTGCAGGTGGATCTCGAGATTTACTCTTACAGTATGTCGTTTAATTTTAATGCAGCCATTGATTAGAATTCATGTGTACTTAAACTAGAGGAGTTTCTAGGTGGAAATATAATGATAGTTTGTGGA
TATTGATGCGGCCTGGAATGGGATCCGAGGGTTACTTCATATTTCTTG

Intron 4 GTGTTCTAGT ... GTACAAGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	72.73	3	9	7
A2	50	4	8	0
A3	67.74	5	11	10
A4	100	0	2	5
A5	62.5	3	7	3
A6	68.75	20	50	38
A7	60.33	24	53	20
A8	57.45	20	33	21
A9	69.57	14	35	29
A10	71.43	13	32	33

>TRINITY_DN44896_c1_g3_i10 from sample A6 / Example spliced isoform

CGAGCCCTTGAATGATAACGGCATTTTTTGAATCATTATCAAAGGGAGCGTCCAATGTCACAGGGAGGGAGGAGCCATATGTGATGGCCCGAATGTCGAATTCAGAAAGCAGTGGGTGAACGTCCTCCTTGTGGCCTTCGAACGGCCTGATGGCATTTGTCTGCCGAAACGCGTAGGACTCTCCTAGACAAGATCCAAGC
CGCGCCTCTTGTAGATGTACTTCTGGTACGCCGGATCCTTCAAAATGGTACCGTCCATATCGAAGACGAGCGTATGGCGCCAAATTGAGAACCATGTGTTAGAAGGGCGTAAGCCAAAGAACAAGTCACCTTGCCCAGTCAAATCCATTTGAAACCTGCATCGTCTTGCTGGGTCTGCCCTCTTGATGCCTCACTCGCCT
CCAGGTACATCTGGAGATTTATTCTTACTATGCGAGCGGTAATTGAAATGTTTGCAGTAATTAATCCG

>TRINITY_DN44896_c1_g3_i7 from sample A6 / Example unspliced isoform:

CGAGCCCTTGAATGATAACGGCATTTTTTGAATCATTATCAAAGGGAGCGTCCAATGTCACAGGGAGGGAGGAGCCATATGTGATGGCCCGAATGTCGAATTCAGAAAGCAGTGGGTGAACGTCCTCCTTGTGGCCTTCGAACGGCCTGATGGCATTTGTCTGCCGAAACGCGTAGGACTCTCCTAGACAAGATCCAAGC
CGCGCCTCTTGTAGATGTACTTCTGGTACGCCGGATCCTTCAAAATGGTACCGTCCATATCGAAGACGAGCGTATGGCGCCAAATTGAGAACCATGTGTTAGAAGGGCGTAAGCCAAAGAACAAGTCACCTTGCCCAGTCAAATCCATTTGAAACCTGCATCGTCTTGCTGGGTCTGCCCTCTTGATGCCTCACTCGCCT
CCAGgtgttctagtaattttttgtatggtgggtttattttattggggaatgaatgtccaggttggccggttggcccttaatggtacaagaagGTACATCTGGAGATTTATTCTTACTATGCGAGCGGTAATTGAAATGTTTGCAGTAATTAATCCG

Intron 5 GTGTTCTATT ... GAATGTCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	63.87	76.3	141.5	130.3
A2	62.69	56	92.5	103
A3	62.41	68.5	112	125
A4	68.75	33.5	74.5	79.5
A5	72.8	14.5	40.5	39
A6	63.54	105.8	179	210.5
A7	52.36	461.3	455.5	631.8
A8	56.6	385	470.5	592.8
A9	57.56	363.3	491.5	559.3
A10	60.5	336.8	489	595

>TRINITY_DN44497_c0_g1_i8 from sample A9 / Example spliced isoform

CAGGAATATCTGGGTTTGTATATGCAGCGGGCTCGTTGGCTAGTCGGGTATCCATTTTGTTAGATGAATGCAGCATGGCATGCAACAAAGGGATCAGCTCCTCCTCCCTTCTTCAAAGGTTGGCCGGTTGGCCCTTAATGGTACAAGAAGGTATAGGCCTGGACCAAAAGGTGCATCTGGAGATTTAGTCTTACTATGCG
AGCGGTATTTGAAATGTTTGCAGATTATGCAGGTATTATGAGAGAAGAAAAACACTTTGATGTAATGTTAGATTATGATGAAGAGTTGAGAATGCTAGAAGAATGGATAGAAAATCTAAGGATTTGTGAAGACATTGATAAAAAGATTGTAGCAGATGAAGAATCTAAAATCATTATTCCAGAATCAGAAATTAGCATTA
CAGAAATCCCCGAGGAAACGAAGTTAAGTAACATACACATGCAAGGAATAGAGCTGAACATTGAGATAGAAGAAATGCAAGGAATAGAGTTGAACATTGAGATAGAAGAATTATGGAAATGTTTGCAGAATACTTTTGGGTGGAGACACAATGCCACAAAGGATATAGTTAACCACAAAGATCAGTTGATTGAAGATACT
TTTGAAAGCAAGGCAAGAGGACGACAACAACATCAGATTTGGGATCCATGCGAACATCAGATTCTATATTTGAAGATGAAGATGAAAGAAATAGGCCTAAGTAGCCTAATGTGAAGCTAATTTAGTTTTATGAAATTTTTGTTAAGGGTTTGTAAGCTTGGCCAAGACATAGACAGCAAGCATTTGCATTTGTGAGATTT
GCATTTTAAATTTTTTAGAGTATCATAATTACAAGGGAGTGTTGTGTTTAGTAAGAATGTAACCCTTGAGATTAAGAGGGAGTGTTAGAAATAATAATCTCATTAGTTCAGTGTGTTAGTTACATTCAGTTATTCCGTTCTAAGATCGGTTAGTCGGTGTTTCCTTAAACCGTCAAACTATGTAACATATATATACTAAT
GTGTAATATGAGATAAGTAGTTCAGCCGTTCGTGC

>TRINITY_DN44497_c0_g1_i6 from sample A9 / Example unspliced isoform:

CAGGAATATCTGGGTTTGTATATGCAGCGGGCTCGTTGGCTAGTCGGGTATCCATTTTGTTAGATGAATGCAGCATGGCATGCAACAAAGGGATCAGCTCCTCCTCCCTTCTTCAAAGgtgttctattaatttctttgtatggtgggttttatttgattggggaacgaatgtccagGTTGGCCGGTTGGCCCTTAATGGT
ACAAGAAGGTATAGGCCTGGACCAAAAGGTGCATCTGGAGATTTAGTCTTACTATGCGAGCGGTATTTGAAATGTTTGCAGATTATGCAGGTATTATGAGAGAAGAAAAACACTTTGATGTAATGTTAGATTATGATGAAGAGTTGAGAATGCTAGAAGAATGGATAGAAAATCTAAGGATTTGTGAAGACATTGATAAA
AAGATTGTAGCAGATGAAGAATCTAAAATCATTATTCCAGAATCAGAAATTAGCATTACAGAAATCCCCGAGGAAACGAAGTTAAGTAACATACACATGCAAGGAATAGAGCTGAACATTGAGATAGAAGAAATGCAAGGAATAGAGTTGAACATTGAGATAGAAGAATTATGGAAATGTTTGCAGAATACTTTTGGGTG
GAGACACAATGCCACAAAGGATATAGTTAACCACAAAGATCAGTTGATTGAAGATACTTTTGAAAGCAAGGCAAGAGGACGACAACAACATCAGATTTGGGATCCATGCGAACATCAGATTCTATATTTGAAGATGAAGATGAAAGAAATAGGCCTAAGTAGCCTAATGTGAAGCTAATTTAGTTTTATGAAATTTTTGT
TAAGGGTTTGTAAGCTTGGCCAAGACATAGACAGCAAGCATTTGCATTTGTGAGATTTGCATTTTAAATTTTTTAGAGTATCATAATTACAAGGGAGTGTTGTGTTTAGTAAGAATGTAACCCTTGAGATTAAGAGGGAGTGTTAGAAATAATAATCTCATTAGTTCAGTGTGTTAGTTACATTCAGTTATTCCGTTCTA
AGATCGGTTAGTCGGTGTTTCCTTAAACCGTCAAACTATGTAACATATATATACTAATGTGTAATATGAGATAAGTAGTTCAGCCGTTCGTGC