NA ----> NA

Intron 1 GTACGATCTC ... CATTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	2	0
A2	50	6	0	6
A3	100	0	0	1
A4	0	4	6	0
A5	0	1	0	0
A6	44.44	10	0	8
A7	34.33	44	3	23
A8	43.62	53	1	41
A9	43.57	79	3	61
A10	45.65	25	2	21

>TRINITY_DN33909_c0_g1_i3 from sample A9 / Example spliced isoform

ATATCATCACGGTCTTTGGAATTGAATCGCCATTATGATTTACTGAACTGTCATTCAAATCTGATAGAATAATCCATTCACATGTCATCGCGTTCTTTCGAGTTTAATTATAATCCATTATAGTTTTACTGGGTCAACCAAGCTGCGGAATGTATGAGCCTAGCTGCAAGCCGGCCTTTATAAATTCAAGAGTGTTGAGA
GCGAGCGCTTGCATCATCCAACATACATCTGGGTGGACGCAGGTACGATCTTGATCTTGATCTTGATTCTAGTTATACGCTTAGACGCAGTGGCATTTGATTGATATCATTGACAGTGGAAGTAAGCAAGTACTGTAATCCGCATCTTTATACAGAGGCCAATTACTCGATTTTGAAATGAGGATAGACACTGTGGCATT
GAAAGCACAGATTGACGAAAAGATTGGTCCAAATGCAGCGAAACAATATTCTGAAAACCTCATTATGTTCTTCAGCGGGAGATTGGCCAGAGCCGAGTTCGTCAGTCGTTGCTATTCGATTCTGGGCCCTGAAAATATCTGGTTACACAATCAGCTCATCTTAGCAATTTCGAAGAATGCATCACAGGGTGAAGTTCCGC
CCCCACCTCCTGTGTAACACACTTCGAGTCCCTTTAAAGGTTCTACTCAGAAATCCGATTGACTGTCGCAGTAAATATGATATGCCATGTTATGTATTTATGAGTGAAACAATTATGTCGTCAGTGTAGTTTCAGAATACTTCATGAAATATTGAATATCTTAGTGATATTTTGCTGAGCAGTAATATGCATAGGAGATA
TTAGGATTTCTGCCAAAATGCTGTGCATTTACTATGTTTCAATGCTTATTCTAGCTTCAAGAAAATTAGCAAATATAGTTCATTATGCTATGTTTAGGACAATGTCCTATACCCTACACTAGGACAATGTCATTCATTATAGTTCATTGTCTTTTAATATCTTAACTATGTCATTTAATGTCA

>TRINITY_DN33909_c0_g1_i2 from sample A9 / Example unspliced isoform:

ATATCATCACGGTCTTTGGAATTGAATCGCCATTATGATTTACTGAACTGTCATTCAAATCTGATAGAATAATCCATTCACATGTCATCGCGTTCTTTCGAGTTTAATTATAATCCATTATAGTTTTACTGGGTCAACCAAGCTGCGGAATGTATGAGCCTAGCTGCAAGCCGGCCTTTATAAATTCAAGAGTGTTGAGA
GCGAGCGCTTGCATCATCCAACATACATCTGGGTGGACGCAGGTACGATCTTGATCTTGATCTTGATTCTAGTTATACGCTTAGACGCAGgtacgatctcttgattatccagtctctttattcattgctaacgatcccaaatcttttctgagcatgtcgagtacatgacagtgccctttgttttctaaaggtacagcaaa
tacatgtcaattttaataaatccagttacacataaaactctatgagaagatggattcttgaacttaacaagatgacctttacgttgttctagctgtctagatgacttcagctgataacccataccttgaaaatcatgcatactaaaaagtggtcatagttagctctgagaacccatcataatactaatggtttatagtga
agcctataacaaacttaaagtaatatcatggttttgagcaatcggccgacagatgtttgggcatcaccatagcaagctgtctgtgcggattttgaaaatgtataactctattttcagaactaatttttttttcaaatgaaaaagtttataatttgatgccaattttattctattgctgttgtttgagactgaattttgtt
ctacaggttatctaactatactttctttgcttttcttttcaacattgtacagTGGCATTTGATTGATATCATTGACAGTGGAAGTAAGCAAGTACTGTAATCCGCATCTTTATACAGAGGCCAATTACTCGATTTTGAAATGAGGATAGACACTGTGGCATTGAAAGCACAGATTGACGAAAAGATTGGTCCAAATGCAG
CGAAACAATATTCTGAAAACCTCATTATGTTCTTCAGCGGGAGATTGGCCAGAGCCGAGTTCGTCAGTCGTTGCTATTCGATTCTGGGCCCTGAAAATATCTGGTTACACAATCAGCTCATCTTAGCAATTTCGAAGAATGCATCACAGGGTGAAGTTCCGCCCCCACCTCCTGTGTAACACACTTCGAGTCCCTTTAAA
GGTTCTACTCAGAAATCCGATTGACTGTCGCAGTAAATATGATATGCCATGTTATGTATTTATGAGTGAAACAATTATGTCGTCAGTGTAGTTTCAGAATACTTCATGAAATATTGAATATCTTAGTGATATTTTGCTGAGCAGTAATATGCATAGGAGATATTAGGATTTCTGCCAAAATGCTGTGCATTTACTATGTT
TCAATGCTTATTCTAGCTTCAAGAAAATTAGCAAATATAGTTCATTATGCTATGTTTAGGACAATGTCCTATACCCTACACTAGGACAATGTCATTCATTATAGTTCATTGTCTTTTAATATCTTAACTATGTCATTTAATGTCA

Intron 2 GTACGATCTT ... TTAGACGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	5	0	0
A2	33.33	12	0.8	6
A3	0	6	0.8	0
A4	28.57	10	4.5	4
A5	100	0	1	1
A6	17.86	46	9.8	10
A7	40.74	64	20.3	44
A8	33.54	105	9	53
A9	32.11	167	27.3	79
A10	26.04	71	7.5	25

>TRINITY_DN42235_c0_g1_i3 from sample A6 / Example spliced isoform

TTCACATATCATCACGGTCTTTGGAATTGAATCGCCATTATGATTTACTGAACTGTCATTCAAATCTGATAGAATAATCCATTCACATGTCATCGCGTTCTTTCGAGTTTAATTATAATCCATTATAGTTTTACTGGGTCAACCAAGCTGCGGAATGTATGAGCCTAGCTGCAAGCCGGCCTTTATAAATTCAAGAGTGT
TGAGAGCGAGCGCTTGCATCATCCAACATACATCTGGGTGGACGCAGTGGCATTTGATTGATATCATTGACAGTGGAAGTAAGCAAGTACTGTAATCCGCATCTTTATACAGAGGCCAATTACTCGATTTTGAAATGAGGATAGACACTGTGGCATTGAAAGCACAGATTGACGAAAAGATTGGTCCAAATGCAGCGAAA
CAATATTCTGAAAACCTCATTATGTTCTTCAGCGGGAGATTGGCCAGAGCCGAGTTCGTCAGTCGTTGCTATTCGATTCTGGGCCCTGAAAATATCTGGTTACACAATCAGCTCATCTTAGCAATTTCGAAGAATGCATCACAGGGTGAAGTTCCGCCCCCACCTCCTGTGTAACACACTTCGAGTCCCTTTAAAGGTTC
TACTCAGAAATCCGATTGACTGTCGCAGTAAATATGATATGCCATGTTATGTATTTATGAGTGAAACAATTATGTCGTCAGTGTAGTTTCAGAATACTTCATGAAATATTGAATATCTTAGTGATATTTTGCTGAGCAGTAATATGCATAGGAGATATTAGGATTTCTGCCAAAATGCTGTGCATTTACTATGTTTCAAT
GCTTATTCTAGCTTCAAGAAAATTAGCAAATATAGTTCATTATGCTATGTTTAGGACAATGTCCTATACCCTACACTAGGACAATGTCATTCATTATAGTTCATTGTCTTTTAATATCTTAACTATGTCATTTAATGTCATTTATG

>TRINITY_DN42235_c0_g1_i2 from sample A6 / Example unspliced isoform:

TTCACATATCATCACGGTCTTTGGAATTGAATCGCCATTATGATTTACTGAACTGTCATTCAAATCTGATAGAATAATCCATTCACATGTCATCGCGTTCTTTCGAGTTTAATTATAATCCATTATAGTTTTACTGGGTCAACCAAGCTGCGGAATGTATGAGCCTAGCTGCAAGCCGGCCTTTATAAATTCAAGAGTGT
TGAGAGCGAGCGCTTGCATCATCCAACATACATCTGGGTGGACGCAGgtacgatcttgatcttgatcttgattctagttatacgcttagacgcagTGGCATTTGATTGATATCATTGACAGTGGAAGTAAGCAAGTACTGTAATCCGCATCTTTATACAGAGGCCAATTACTCGATTTTGAAATGAGGATAGACACTGTG
GCATTGAAAGCACAGATTGACGAAAAGATTGGTCCAAATGCAGCGAAACAATATTCTGAAAACCTCATTATGTTCTTCAGCGGGAGATTGGCCAGAGCCGAGTTCGTCAGTCGTTGCTATTCGATTCTGGGCCCTGAAAATATCTGGTTACACAATCAGCTCATCTTAGCAATTTCGAAGAATGCATCACAGGGTGAAGT
TCCGCCCCCACCTCCTGTGTAACACACTTCGAGTCCCTTTAAAGGTTCTACTCAGAAATCCGATTGACTGTCGCAGTAAATATGATATGCCATGTTATGTATTTATGAGTGAAACAATTATGTCGTCAGTGTAGTTTCAGAATACTTCATGAAATATTGAATATCTTAGTGATATTTTGCTGAGCAGTAATATGCATAGG
AGATATTAGGATTTCTGCCAAAATGCTGTGCATTTACTATGTTTCAATGCTTATTCTAGCTTCAAGAAAATTAGCAAATATAGTTCATTATGCTATGTTTAGGACAATGTCCTATACCCTACACTAGGACAATGTCATTCATTATAGTTCATTGTCTTTTAATATCTTAACTATGTCATTTAATGTCATTTATG

Intron 3 GTACGATCTT ... CATTGTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.09	5	1	0
A2	22.58	12	1	6
A3	14.29	6	1	1
A4	37.5	10	12	0
A5	-1	0	0	0
A6	15.6	46	9	8
A7	18.47	64	6	23
A8	19.23	105	9	41
A9	19.9	167	22	61
A10	15.98	71	6	21

>TRINITY_DN33909_c0_g1_i1 from sample A9 / Example spliced isoform

ATATCATCACGGTCTTTGGAATTGAATCGCCATTATGATTTACTGAACTGTCATTCAAATCTGATAGAATAATCCATTCACATGTCATCGCGTTCTTTCGAGTTTAATTATAATCCATTATAGTTTTACTGGGTCAACCAAGCTGCGGAATGTATGAGCCTAGCTGCAAGCCGGCCTTTATAAATTCAAGAGTGTTGAGA
GCGAGCGCTTGCATCATCCAACATACATCTGGGTGGACGCAGTGGCATTTGATTGATATCATTGACAGTGGAAGTAAGCAAGTACTGTAATCCGCATCTTTATACAGAGGCCAATTACTCGATTTTGAAATGAGGATAGACACTGTGGCATTGAAAGCACAGATTGACGAAAAGATTGGTCCAAATGCAGCGAAACAATA
TTCTGAAAACCTCATTATGTTCTTCAGCGGGAGATTGGCCAGAGCCGAGTTCGTCAGTCGTTGCTATTCGATTCTGGGCCCTGAAAATATCTGGTTACACAATCAGCTCATCTTAGCAATTTCGAAGAATGCATCACAGGGTGAAGTTCCGCCCCCACCTCCTGTGTAACACACTTCGAGTCCCTTTAAAGGTTCTACTC
AGAAATCCGATTGACTGTCGCAGTAAATATGATATGCCATGTTATGTATTTATGAGTGAAACAATTATGTCGTCAGTGTAGTTTCAGAATACTTCATGAAATATTGAATATCTTAGTGATATTTTGCTGAGCAGTAATATGCATAGGAGATATTAGGATTTCTGCCAAAATGCTGTGCATTTACTATGTTTCAATGCTTA
TTCTAGCTTCAAGAAAATTAGCAAATATAGTTCATTATGCTATGTTTAGGACAATGTCCTATACCCTACACTAGGACAATGTCATTCATTATAGTTCATTGTCTTTTAATATCTTAACTATGTCATTTAATGTCA

>TRINITY_DN33909_c0_g1_i2 from sample A9 / Example unspliced isoform:

ATATCATCACGGTCTTTGGAATTGAATCGCCATTATGATTTACTGAACTGTCATTCAAATCTGATAGAATAATCCATTCACATGTCATCGCGTTCTTTCGAGTTTAATTATAATCCATTATAGTTTTACTGGGTCAACCAAGCTGCGGAATGTATGAGCCTAGCTGCAAGCCGGCCTTTATAAATTCAAGAGTGTTGAGA
GCGAGCGCTTGCATCATCCAACATACATCTGGGTGGACGCAGgtacgatcttgatcttgatcttgattctagttatacgcttagacgcaggtacgatctcttgattatccagtctctttattcattgctaacgatcccaaatcttttctgagcatgtcgagtacatgacagtgccctttgttttctaaaggtacagcaaa
tacatgtcaattttaataaatccagttacacataaaactctatgagaagatggattcttgaacttaacaagatgacctttacgttgttctagctgtctagatgacttcagctgataacccataccttgaaaatcatgcatactaaaaagtggtcatagttagctctgagaacccatcataatactaatggtttatagtga
agcctataacaaacttaaagtaatatcatggttttgagcaatcggccgacagatgtttgggcatcaccatagcaagctgtctgtgcggattttgaaaatgtataactctattttcagaactaatttttttttcaaatgaaaaagtttataatttgatgccaattttattctattgctgttgtttgagactgaattttgtt
ctacaggttatctaactatactttctttgcttttcttttcaacattgtacagTGGCATTTGATTGATATCATTGACAGTGGAAGTAAGCAAGTACTGTAATCCGCATCTTTATACAGAGGCCAATTACTCGATTTTGAAATGAGGATAGACACTGTGGCATTGAAAGCACAGATTGACGAAAAGATTGGTCCAAATGCAG
CGAAACAATATTCTGAAAACCTCATTATGTTCTTCAGCGGGAGATTGGCCAGAGCCGAGTTCGTCAGTCGTTGCTATTCGATTCTGGGCCCTGAAAATATCTGGTTACACAATCAGCTCATCTTAGCAATTTCGAAGAATGCATCACAGGGTGAAGTTCCGCCCCCACCTCCTGTGTAACACACTTCGAGTCCCTTTAAA
GGTTCTACTCAGAAATCCGATTGACTGTCGCAGTAAATATGATATGCCATGTTATGTATTTATGAGTGAAACAATTATGTCGTCAGTGTAGTTTCAGAATACTTCATGAAATATTGAATATCTTAGTGATATTTTGCTGAGCAGTAATATGCATAGGAGATATTAGGATTTCTGCCAAAATGCTGTGCATTTACTATGTT
TCAATGCTTATTCTAGCTTCAAGAAAATTAGCAAATATAGTTCATTATGCTATGTTTAGGACAATGTCCTATACCCTACACTAGGACAATGTCATTCATTATAGTTCATTGTCTTTTAATATCTTAACTATGTCATTTAATGTCA