NA ----> NA

Intron 1 GCAAGTTGTT ... GTATTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.15	14	5	12
A2	52	12	0	13
A3	48.84	22	0	21
A4	54.55	5	2	6
A5	57.14	6	0	8
A6	47.97	32	4	29.5
A7	47.32	59	8	53
A8	39.68	38	10	25
A9	42.86	28	7	21
A10	43.33	34	5	26

>TRINITY_DN45420_c0_g1_i3 from sample A6 / Example spliced isoform

CCCTTCCAGGTGATCAGTCCAGGGGATTATTGGATGACCCGTAGGCCTAAAAATTGTTGTACTATTAAGGTGTAATTTAATTAAAAGGGGATTATTTCAGTAGATGGTATCGGATGAACCATGGTAGTGCCCATGGTATGGAGTTCTGGGAGAAGATAAATGGAGAAGAGCACCTCACTTGTGGGATATGTTGATTTGAT
TTGATCTTAATCCTGGTGAGTTGGGATTTTTCCTTAGGATTTGGAAAAACCCTTACATGTTGGTTTAGGTTCGGTGGTGCAAGACAAAGAGGGGAATCCAATCAGGTTTTAGTTGAGCTTATTTGGTGATTCTATCTTCAATTTTGTAAGTAGTGTCTTGGCTTGGGCGATTTGTTAAGGAAGCCAAGATCATCATTGTG
GCGTAGCCATGGCATCTCGAGGTCATGGCCTTGGCATATGAGAGGCCAAATTTTATAAGGAAGCCATGGGTTTGAGGGCGCACCAACTGTAGAGGAAATTCCAGCTTCATTCATCGTTCATTGTGTACACGGGGTTCCCACTTGTGTTTCAATTGGTGCTTCATCTTCAGTTTTCAGTGTGTAGCGATTTGGTTGGAGAT
ATCCCTAGGAAGGGCAAATATATCCCTTGAGGGTCGAATTCCATTGTGAAACTGGATTGCAAGAAGGGAGTCAAGCTTGATGTGAAGTTTGAGTTTGCTGTGGGGACCGATCTCACTTGAGTTTGCTGTAGGGGCCAATCTCACTTGAGGAGGTCGATCTCACTTGTGAAGGACGAAATTGCTGAGCCGCATTCATCCAA
GGACGAGTTTCTTCCTCATTGGGCCGAATTCATTATGAATTGGGGGCGAAATTGAAAGGGGTCGAGTTTCTTGTTGAGGGCAGCCAAATTCCATAAGAGACAAGGAGACCTTGTGTTCATTTCGTGGCTCTTAGGGATCGTCGGGGTTCGAGTAAGGGGTCGACCCCATCTCATGTGCTTGGCAGGTTGGAGAGAGGCCG
CTTCTCTCTAAGGAAGGCCGCAACTTTGTGGGATAGCAGCCGCGGACTTGAGGAAGGGTCGTGAGGGTCAAGTCACCATTTAGCAGGCTGCAGGGGTTTGAGAAAGAAGCCGCCTGCATTGTGGAGTGTCAGTCGTCTTTTTTGAAGCATTTGGGAAGGCATCTTCACTTGGGATTCGACGGCACAACTTTGGGTGCCTT
TGGAGGCAACTTGAAAACATTCCCAGGTGAGTTCAAGTGGCTGACACATGTTGGACCATATACAACCAGGACATCACCCTCAAAGATATTACTTGGCTGTCTTGGGCAAAGATCAATGATTGAGGAAGGTGGACTGTTACTTGCTTTTAAGTTGTTAAATATAGGGAAAGGATAGGCAGAGAAAGAGAAAGGGCCTGTCG
ACAGTAGCAAAAGCTATTTGAATCAATGTTGAAGGTGATGTTTATTTAGAGGATTCTAAAGTAGCACTAGAGAATGTTATTGAACTAGATACTGGAAAGGCAGGAACTAGAAAGAGAATTTAATTAAGAGATGAAGCCATCTTCAAAACCTAGAA

>TRINITY_DN45420_c0_g1_i1 from sample A6 / Example unspliced isoform:

CCCTTCCAGGTGATCAGTCCAGGGGATTATTGGATGACCCGTAGGCCTAAAAATTGTTGTACTATTAAGGTGTAATTTAATTAAAAGGGGATTATTTCAGTAGATGGTATCGGATGAACCATGGTAGTGCCCATGGTATGGAGTTCTGGGAGAAGATAAATGGAGAAGAGCACCTCACTTGTGGGATATGTTGATTTGAT
TTGATCTTAATCCTGGTGAGTTGGGATTTTTCCTTAGGATTTGGAAAAACCCTTACATGTTGGTTTAGGTTCGGTGGTGCAAGACAAAGAGGGGAATCCAATCAGGTTTTAGTTGAGCTTATTTGGTGATTCTATCTTCAATTTTGTAAGTAGTGTCTTGGCTTGGGCGATTTGTTAAGGAAGCCAAGATCATCATTGTG
GCGTAGCCATGGCATCTCGAGGTCATGGCCTTGGCATATGAGAGGCCAAATTTTATAAGGAAGCCATGGGTTTGAGGGCGCACCAACTGTAGAGGAAATTCCAGCTTCATTCATCGTTCATTGTGTACACGGGGTTCCCACTTGTGTTTCAATTGGTGCTTCATCTTCAGTTTTCAGTGTGTAGCGATTTGGTTGGAGAT
ATCCCTAGGAAGGGCAAATATATCCCTTGAGGGTCGAATTCCATTGTGAAACTGGATTGCAAGAAGGGAGTCAAGCTTGATGTGAAGTTTGAGTTTGCTGTGGGGACCGATCTCACTTGAGTTTGCTGTAGGGGCCAATCTCACTTGAGGAGGTCGATCTCACTTGTGAAGGACGAAATTGCTGAGCCGCATTCATCCAA
GGACGAGTTTCTTCCTCATTGGGCCGAATTCATTATGAATTGGGGGCGAAATTGAAAGGGGTCGAGTTTCTTGTTGAGGGCAGCCAAATTCCATAAGAGACAAGGAGACCTTGTGTTCATTTCGTGGCTCTTAGGGATCGTCGGGGTTCGAGTAAGGGGTCGACCCCATCTCATGTGCTTGGCAGGTTGGAGAGAGGCCG
CTTCTCTCTAAGGAAGGCCGCAACTTTGTGGGATAGCAGCCGCGGACTTGAGGAAGGGTCGTGAGGGTCAAGTCACCATTTAGCAGGCTGCAGGGGTTTGAGAAAGAAGCCGCCTGCATTGTGGAGTGTCAGTCGTCTTTTTTGAAGCATTTGGGAAGGCATCTTCACTTGGGATTCGACGGCACAACTTTGGGTGCCTT
TGGAGGCAACTTGAAAACATTCCCAGGTGAGTTCAAGTGGCTGACACATGTTGGACCATATACAACCAGGACATCACCCTCAAAGATATTACTTGGCTGTCTTGGGCAAAGATCAATGATTGAGGAAGGTGGACTGTTACTTGCTTTTAAGTTGTTAAATATAGgcaagttgttatataagcaatctgttacttgcctta
ttttcaacaagtgttcttccttccagttccttccaattttattgtcttattttcatgttttggctccttctatagtaataaatattgtggtataatttgtattaccagGGAAAGGATAGGCAGAGAAAGAGAAAGGGCCTGTCGACAGTAGCAAAAGCTATTTGAATCAATGTTGAAGGTGATGTTTATTTAGAGGATTC
TAAAGTAGCACTAGAGAATGTTATTGAACTAGATACTGGAAAGGCAGGAACTAGAAAGGTTAGTTTGTTTCTTTTTAGTATTTGATTGGGAGTAAAGAATATAGAAAACAAATTATCTTGAAGGTTTATTTGTATTATTTGTTTGTTATTGCAATTTTTTAGTTTTGCTTGTAAAATGCTCATATGTTAGACATTTTGTG
GGGATCTGGAAACTGATGTTTTAGAAGATGCTCTAAACTTTGTAGAGAATTTAATTAAGAGATGAA

Intron 2 GTGGACTGTT ... TTAAATATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.58	13.5	6	14
A2	31.58	13	0	12
A3	38.81	20.5	4	22
A4	42.86	4	1	5
A5	31.66	6.5	0	6
A6	45.66	27.5	15	31
A7	41.08	54.5	17	59
A8	59.41	20.5	22	38
A9	45.67	25	14	28
A10	48.96	24	12	34

>TRINITY_DN48797_c1_g5_i3 from sample A7 / Example spliced isoform

TTGAGCCGAAGCTAAGTATAAGTGTGTTCCAGTCAGAGGTAGCTGTTATAAAACTGTTGTTTTATTTTTTCAAAAACAAAAGCAACAAGTCTGTTGACTGGCATTAGTGCGCCATTGCAGATCAGAGTTACAAAACAAGGTCAACAATGGCATGTATTTCTAGCTAGTTTTGTAAAATCTGCCTTAAAGCTATGGGTTGT
TGTAGTGCACAAGGTGAGCCATTGCGAAACACATAGTTAAAATCCATGCAAAGGTTGCAATGACTTCTACTTCCATCTGGTTTATTGAAATCCACCTTGAAGCTGTAGGTTAATGTATAGAACAGGGTGTGTTATCATGCATTTTACGAAACAATGAAAAATGTCCCACTGATATACACTTCTGCTTAGCTTCGGTTCAA
TGGTATACTTATCTGTCATCTCCTAAATCTCGTTACCTTTGTACCTGTTTTCGAGGGAACTGTTACGAAACTGCGATTTTATTTTAAAAAAAAAACTAGTCTGTGACTGGCATTAGTTTGCCATTGCAAATCAGAGTTAAAAAAACATGCCATGGACCATGGTCACCATGACGTGTATTTACAGCCAGCTTCATTGTGCA
GCTCACTAGTGACAGATAGGATGCAACTAGTGGCCAATGATACATGTCACTAGTTATACAAAAACATGTATGTACAACCAGTTTCATTGTGCCAATGTGCAGCTAACTAGTGACAGCTAGGCTGCAGCCTGCAACTAGTGGCCAATGATATATAACTTGCAATATGTATACAACAATAACCCCTCTTGTACACAGTTTTC
TGTTTTAGTACAAAGTCTGTAATTTGCTCACTTGAACCACATGAGAAATGAGGCATAAAATGGCCCATGAGATTGTTGCAAATGGTCACAGAGATAGTTAGCTGGCCGTATTTGTCAAATCAAATCAGCATGCACCAGTTGTGTCTTTGCAGGTGAGTTCAAGTGGCTGACACATGTTGGACCATATACAACCAGGACAT
CACCCTCAAAGATATTACTTGGCTGTCTTGGGCAAAGATCAATGATTGAGGAAGGGAAAGGATAGGCAGAGAAAGAGAAAGGGCCTGTCGACAGTAGCAAAAGCTATTTGAATCAATGTTGAAGGTGATGTTTATTTAGAGGATTCTAAAGTAGCACTAGAGAATGTTATTGAACTAGATACTGGAAAGGCAGGAACTAG
AAAGGTTAGTTTGTTTCTTTTTAGTATTTGATTGGGAGTAAAGAATATAGAAAACAAATTATCTTGAAGGTTTATTTGTATTATTTGTTTGTTATTGCAATTTTTTAGTTTTGCTTGTAAAATGCTCATATGTTAGACATTTTGTGGGGATCTGGAAACTGATGTTTTAGAAGATGCTCTAAACTTTGTAGAGAATTTAA
TTAAGAGATGAAGCCATCTTCAAAACCTAGAAATGCACAGAAGATTGTCAAGGATTCAGAAATATCTCTAGAGCAAATGGATCCCAAGCATTTGAGAATAGATGGTGATTGTAAACAGAAAAGAAGACCTGGGAAGAGAATGGAGAGTAGTGCAAATGTTGGTTCACAATCAGGCAGACCTGCTGAGAAAGTTGCTGAAC
CTGTGGAATCCCCTTCTTTTGTATTTCTGTTGGTTTAGTAATCCTTTAGCACTAGCCTTTACTTTCATTTCATGCTATATTGTATGCTGCCAAGAAC

>TRINITY_DN48797_c1_g5_i5 from sample A7 / Example unspliced isoform:

TTACAAAATAACGAAAAAAGGTCCGATATACACTTGTGCTTAGCTTCTGTTCAATGGCATAATTATCTATCATCTTGTTATGTTCCCTTCCAGGTGATCAGTCCAGGGGATTATTGGATGACCCGTAGGCCTAAAAATTGTTGTACTATTAAGGTGTAATTTAATTAAAAGGGGATTATTTCAGTAGATGGTATCGGATG
AACCATGGTAGTGCCCATGGTATGGAGTTCTGGGAGAAGATAAATGGAGAAGAGCACCTCACTTGTGGGATATGTTGATTTGATTTGATCTTAATCCTGGTGAGTTGGGATTTTTCCTTAGGATTTGGAAAAACCCTTACATGTTGGTTTAGGTTCGGTGGTGCAAGACAAAGAGGGGAATCCAATCAGGTTTTAGTTGA
GCTTATTTGGTGATTCTATCTTCAATTTTGTAAGTAGTGTCTTGGCTTGGGCGATTTGTTAAGGAAGCCAAGATCATCATTGTGGCGTAGCCATGGCATCTCGAGGTCATGGCCTTGGCATATGAGAGGCCAAATTTTATAAGGAAGCCATGGGTTTGAGGGCGCACCAACTGTAGAGGAAATTCCAGCTTCATTCATCG
TTCATTGTGTACACGGGGTTCCCACTTGTGTTTCAATTGGTGCTTCATCTTCAGTTTTCAGTGTGTAGCGATTTGGTTGGAGATATCCCTAGGAAGGGCAAATATATCCCTTGAGGGTCGAATTCCATTGTGAAACTGGATTGCAAGAAGGGAGTCAAGCTTGATGTGAAGTTTGAGTTTGCTGTGGGGACCGATCTCAC
TTGAGTTTGCTGTAGGGGCCAATCTCACTTGAGGAGGTCGATCTCACTTGAGAAGGACGAAATTGCTGAGCCGCATTCATCCAAGGACGAGTTTCTTCCTCATTGGGCCGAATTCATTATGAATTGGGGGCGAAATTGAAAGGGGTCGAGTTTCTTGTTGAGGGCAGCCAAATTCCATAAGAGACAAGGAGACCTTGTGT
TCATTTCGTGGCTCTTAGGGATCGTCGGGGTTCGAGTAAGGGGTCGACCCCATCTCATGTGCTTGGCAGGTTGGAGAGAGGCCGCTTCTCTCTAAGGAAGGCCGCAACTTTGTGGGATAGCAGCCGCGGACTTGAGGAAGGGTCGTGAGGGTCAAGTCACCATTTAGCAGGCTGCAGGGGTTTGAGAAAGAAGCCGCCTG
CATTGTGGAGTGTCAGTCGTCTTTTTTGAAGCATTTGGGAAGGCATCTTCACTTGGGATTCGACGGCACAACTTTGGGTGCCTTTGGAGGCAACTTGAAAACATTCCCAGGTGAGTTCAAGTGGCTGACACATGTTGGACCATATACAACCAGGACATCACCCTCAAAGATATTACTTGGCTGTCTTGGGCAAAGATCAA
TGATTGAGGAAGgtggactgttacttgcttttaagttgttaaatatagGGAAAGGATAGGCAGAGAAAGAGAAAGGGCCTGTCGACAGTAGCAAAAGCTATTTGAATCAATGTTGAAGGTGATGTTTATTTAGAGGATTCTAAAGTAGCACTAGAGAATGTTATTGAACTAGATACTGGAAAGGCAGGAACTAGAAAGGT
TAGTTTGTTTCTTTTTAGTATTTGATTGGGAGTAAAGAATATAGAAAACAAATTATCTTGAAGGTTTATTTGTATTATTTGTTTGTTATTGCAATTTTTTAGTTTTGCTTGTAAAATGCTCATATGTTAGACATTTTGTGGGGATCTGGAAACTGATGTTTTAGAAGATGCTCTAAACTTTGTAGAGAATTTAATTAAGA
GATGAAGCCATCTTCAAAACCTAGAAATGCACAGAAGATTGTCAAGGATTCAGAAATATCTCTAGAGCAAATGGATCCCAAGCATTTGAGAATAGATGGTGATTGTAAACAGAAAAGAAGACCTGGGAAGAGAATGGAGAGTAGTGCAAATGTTGGTTCACAATCAGGCAGACCTGCTGAGAAAGTTGCTGAACCTGTGG
AATCCCCTTCTTTTGTATTTCTGTTGGTTTAGTAATCCTTTAGCACTAGCCTTTACTTTCATTTCATGCTATATTGTATGCTGCCAAGAAC

Intron 3 GTTAGTTTGT ... AACTTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.68	74.3	22.4	11.5
A2	15.25	59.9	10.9	13.3
A3	13.04	95.8	13.9	15
A4	19.49	57.3	12.5	13.1
A5	32.78	18.1	8.8	2.6
A6	21.81	135.8	36.8	25.1
A7	21.02	201.6	53	32.6
A8	22.91	186.5	53.9	38.6
A9	25.34	132.4	44.5	18.9
A10	23.67	129	40.3	18.4

>TRINITY_DN134833_c1_g4_i2 from sample A1 / Example spliced isoform

CAGGGTAAGGATAGGCAGAGAAAGAGAAAGGGCATGTCCAGTGCAGCAAAAGCTATTCGAGTCAATGCTGAAGGTGATGTTTATTTAGAGGATTCTAAAGTAGCACTAGAGAATGTTATTGAACTAGATACTGGAAAGGCAGGAACTAGAAAGAGAATTTAATTAAGAGATGAAGCCATCTTCAAAACCTAGAAATGCAC
AGAAGATTGTCAAGGATTCAGAAATATCTCTAGAGCAAATGGATCCCAAGCATTTGAGAATAGATGGTGATTGTAAACAGAAAAGAAGACCTGGGAAGAGAATGGAGAGTAGTGCAAATGTTGGTTCACAATCAGGCAGACCTGCTGAGAAAGTTGCTGAACCTGTGGAATCCCCTTCTTTTGTATTTCTGTTGGTTTAG
TAATCCTTTAGCACTAGCCTTTACTTTCATTTCATGCTATATTGTATGCTGCCAAGAACAGTTATGTATTGATGATGAATTTTCTCATAAGCATCTTTATCTCCTTTTATTGACACCTTTACTTTTAGCATTTTATTAGCACGACAATCTTTAATTGTTGGGTACAAAGAGCCGGA

>TRINITY_DN134833_c1_g4_i1 from sample A1 / Example unspliced isoform:

CAGGGTAAGGATAGGCAGAGAAAGAGAAAGGGCATGTCCAGTGCAGCAAAAGCTATTCGAGTCAATGCTGAAGGTGATGTTTATTTAGAGGATTCTAAAGTAGCACTAGAGAATGTTATTGAACTAGATACTGGAAAGGCAGGAACTAGAAAGgttagtttgtttctttttagtatttgattgggagtaaagaatataga
aaacaaattatcttgaaggtttatttgtattatttgtttgttattgcaattttttagttttgcttgtaaaatgctcatatgttagacattttgtggggatctggaaactgatgttttagaagatgctctaaactttgtagAGAATTTAATTAAGAGATGAAGCCATCTTCAAAACCTAGAAATGCACAGAAGATTGTCAA
GGATTCAGAAATATCTCTAGAGCAAATGGATCCCAAGCATTTGAGAATAGATGGTGATTGTAAACAGAAAAGAAGACCTGGGAAGAGAATGGAGAGTAGTGCAAATGTTGGTTCACAATCAGGCAGACCTGCTGAGAAAGTTGCTGAACCTGTGGAATCCCCTTCTTTTGTATTTCTGTTGGTTTAGTAATCCTTTAGCA
CTAGCCTTTACTTTCATTTCATGCTATATTGTATGCTGCCAAGAACAGTTATGTATTGATGATGAATTTTCTCATAAGCATCTTTATCTCCTTTTATTGACACCTTTACTTTTAGCATTTTATTAGCACGACAATCTTTAATTGTTGGGTACAAAGAGCCGGA