sp|Q8GWG0|GPAT9_ARATH ----> Glycerol-3-phosphate acyltransferase 9

Intron 1 GTTCTACGTT ... TTCAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	80.65	24	85	115
A2	77.78	23	100	61
A3	80.09	22	96	81
A4	72.6	20	46	60
A5	72.65	16	47	38
A6	64.57	127	290	173
A7	66.27	155	421	188
A8	61.16	174	340	208
A9	60.26	210	415	222
A10	66.53	166	418	242

>TRINITY_DN43913_c0_g1_i13 from sample A9 / Example spliced isoform

GGGGGAGGGAAAATGGAAATGCATATGCAATTGCTCTGTTGAGGTCAAGGGTGAGAGCAGAGGGGGGAGGAATTAGAATTGGGATGGAAAAGAAGTTGAAGACTAACCACCAACTGCGGCACAGGAGACCTAGGGTTTAGTATTTTGGATTGTTGAAGAATGGCAGCCTTTTGACAGTTACATATGCTCCCAACACAGGG
AACTGCAGTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCTGAACCTGGGTCTATTTCAACGGATTTGATTGAATCATGCAGCATAGCTTATTACTATTGTTGTTTCTACTAAGCGAAATTGCAGAGTAGAGGAACCCGATTTTTTTTTCTCACAGAAGGCATTAGGGAGGCTGCTTTGGAGTGAACTGCTTG
AAGTCTTTACTTTCCAGCAAGGAGGCCCATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGTTGTTTTGAAAACGGAGTAAATTTGTTTATTGTAATTTGAGGGATCAGGAAACCAGTTTACTTGGTGGACTGGATTGGCTCTACAATGTTCTGTGCTATGCATCTAGAGGATTTTTATTTGCATCTACTGTCGGAAAC
AAGAGTGTTGTTATTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGAGCAATTACATAACTTCAAAATAAGGGACAGGAACTATCATTTTATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGTTTTCAAAAAGGAGGGTGCTGTCGCGGCACTGAGA
GTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCTGGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACACCATCAGACAATACCGACGCTCCATTGGCATCTA
GACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCTGTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACATTCGCGTGCCTATACTAGTTTTCGTCGCAGTTCT
TCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTGAGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTGCTGGCAGGTTTGAATCTCTAAGGCGGTCCCAATC
TGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGTGAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATCATTCAATCTAACCTGGCAAATGTTGCTGGGATGA
ACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTGCTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCAAATTGCACCAGGAAGCTTAGTTTCATCCAAAGGC
CATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAAAGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTATGGGTCTTAGCCCTTCTGAAAAGTCAAAGGCAAA
GTCATTGTGGTCAATTGATACAACTGCTTCTGCTGTTAAAGGTATTCAATCAGTAGGATCATCCCATTCTATGAACTCTCCCAAAGTTACTCAGGGTGGGAAGCTTCTAGTTCTCAAGCCAGCAAGGGATTCGGGATCTTCTGTGCCTGTCAAACCTAATAATGGCAGTCCAGTGAAGGGAGGCAATACCAGTAGCAGCA
GCTCATTTGGTGTATCAGTCAGAGGTGCTATTGATGTCAGTGGAAATGCTAGTCTGGTGGGTTTTAGGAAGCAGAAACCCCTCGTTGACCCCAGAGCAGTATTCTCATCTGGAGTGGATAGTGGGCTAGGGACACGGGGAAAGGAGGCCAACTTATTGATTGAGGAAAAAAAGTCTGTGTCACAAGCACAAAATCGGAGT
GATTTTTTCAATGCATTGAGGAGGAAAGCTTGTGAGAGCCCCAATTCCACTGCAACTCAGGACAAGTCTAATACATCAACAGTGCATAAAGCTGATCGAGAAAATGAGAATATGCTCCTGAGCACCAAGGATTTATTAGAACTAAAGGATAAGTGTTCTGTTGAGAATGTAGGTGAGAAATCTGATACTGTTCCTTCAAT
GGTTGAAACAGAAGCCACTGAGAAAAATAAAACTATCTTGAATGGTTTTGTTACTGTAGAAAGTGATATTGCTATTAGGGATACCAGTGAAACTGGCAGTCCAGCATCTGTCAACAGGGTTTCTGCTAATGTTGGTTCTGAGGAGGAAGAAGCAGCCTTTCTTCGCTCACTTGGGTGGGAAGAAAATGCTAGTGGAGAAG
AGGCACTCACAGAGGAGGAAATCAATTCTTTTTACCGGGAGCACATGAGGGCAGTAGCTGCTAATGCTTTTCGCCGAGGCAGCCATAGACATTGGAACCTGAGATTTGATATGCATGTTGGAAATAATGTGGAGAGCTTTTCTTCTGGTATAGGCTCTTCAGAGTCAGCTTAGATACAGGGTGACACTCATATGGGAAAG
AAAAACTAATAGCGTGGCAGGGATTAGATAGATATAAGATTTGGAAAATTTTGGATTGACTGAAAACATGAAAAAAACTGGCTCTTACCCATGTGTTGCTTCAGAAGGTGCAATCATAAAGAGGTTGGGTAACGAAGTTCCCTTGAGAGCTTTAGATTGTCAACTTGACAAAGTAGATTGCTGTCAAAGTTGGCAAACGA
CAACTTATTTTGGATGAATAGATAATTTTGCAATGTGTGCATATGAAATATTTGTCACAAGAACAGCACAGAATTATGAAATGCAAAGAACTGTGCTCATTAGTAATTTCCATGGTTTTACTCGGTGGTTTTACTCAGAAAGATGTATATGATTCTTGACTTCTGCTGTTCATGCAGAAACGTGGCAGTGAATGTATATT
CATGATGCAAAGTGTCCTTTTCCCAGGTATAGTGTTTGTGCCTTTAGCCAATTTGTGATTTTGGTTTCAGAGTAGCAAGATTGGTGGGATGCTTCAACAACCTCACTCTGAAATTTAATGGGCTGGGAATCATCTTCTTCGTCTTACTGATTCATTTTTGCTGGGAAGAAAGCTTTGGTCCTCTGCTGGTTAGTCTCTCA
TTGACACTTAAAACTAGAATATTCCAATTTTTGAGGTATACAAGTTGGAAAGATTGTATTGGGATACGAATCAATTTTGTAATCTTCTGAACTGCGATTTGTTTTATTTATTCAAAAAGGTATTATTCTTTTTAGTTGACATTTCAGTTCAGGACTTGAATTTGTATTTGAGGATACATACATAATACATTGCCTGCCAT
ATGACCGAAACATGAATATAACAGCAACAGTAACACTGAATATTAATAACGGAAGAAGTGCAGTCAGTGAAGTAATAGAGCGAATTTCTCACATTAAATGAGTTCCTGGCATCTTGCAGACAAACAGTTTAGATGTCATTCTGAAACATGCGTATCTCTTGCATGGTTTTTAATTAGTAATCTGATTGCATGATATTTTA
A

>TRINITY_DN43913_c0_g1_i20 from sample A9 / Example unspliced isoform:

GGGGGAGGGAAAATGGAAATGCATATGCAATTGCTCTGTTGAGGTCAAGGGTGAGAGCAGAGGGGGGAGGAATTAGAATTGGGATGGAAAAGAAGTTGAAGACTAACCACCAACTGCGGCACAGGAGACCTAGGGTTTAGTATTTTGGATTGTTGAAGAATGGCAGCCTTTTGACAGTTACATATGCTCCCAACACAGGG
AACTGCAGgttctacgtttctttttatttctctccctctgttttctttctcggttgaaccccaggggccatttaatttacaaaggctagaaccgtgtcttcaatgcagTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCTGAACCTGGGTCTATTTCAACGGATTTGATTGAATCATGCAGCATAGCTTATT
ACTATTGTTGTTTCTACTAAGCGAAATTGCAGAGTAGAGGAACCCGATTTTTTTTTCTCACAGAAGGCATTAGGGAGGCTGCTTTGGAGTGAACTGCTTGAAGTCTTTACTTTCCAGCAAGGAGGCCCATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGTTGTTTTGAAAACGGAGTAAATTTGTTTATTGTAATTT
GAGGGATCAGGAAACCAGTTTACTTGGTGGACTGGATTGGCTCTACAATGTTCTGTGCTATGCATCTAGAGGATTTTTATTTGCATCTACTGTCGGAAACAAGAGTGTTGTTATTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGAGCAATTACATAACTTCAAAATAAGGGACAGGAACTATCATTTTATT
TAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGTTTTCAAAAAGGAGGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCTGGTT
CCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACACCATCAGACAATACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCTGTCG
TAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACATTCGCGTGCCTATACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTGAGTG
GAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTGCTGGCAGGTTTGAATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGTGAGT
AACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATCATTCAATCTAACCTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTGCTTT
AGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCAAATTGCACCAGGAAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAAAGGA
TGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTATGGGTCTTAGCCCTTCTGAAAAGTCAAAGGCAAAGTCATTGTGGTCAATTGATACAACTGCTTCTGCTGTTAAAGGTATTCAATCAGTAGGATCATCCCATTCTATGAACTCTCCCAAAGTTACTCAGGGTGGG
AAGCTTCTAGTTCTCAAGCCAGCAAGGGATTCGGGATCTTCTGTGCCTGTCAAACCTAATAATGGCAGTCCAGTGAAGGGAGGCAATACCAGTAGCAGCAGCTCATTTGGTGTATCAGTCAGAGGTGCTATTGATGTCAGTGGAAATGCTAGTCTGGTGGGTTTTAGGAAGCAGAAACCCCTCGTTGACCCCAGAGCAGT
ATTCTCATCTGGAGTGGATAGTGGGCTAGGGACACGGGGAAAGGAGGCCAACTTATTGATTGAGGAAAAAAAGTCTGTGTCACAAGCACAAAATCGGAGTGATTTTTTCAATGCATTGAGGAGGAAAGCTTGTGAGAGCCCCAATTCCACTGCAACTCAGGACAAGTCTAATACATCAACAGTGCATAAAGCTGATCGAG
AAAATGAGAATATGCTCCTGAGCACCAAGGATTTATTAGAACTAAAGGATAAGTGTTCTGTTGAGAATGTAGGTGAGAAATCTGATACTGTTCCTTCAATGGTTGAAACAGAAGCCACTGAGAAAAATAAAACTATCTTGAATGGTTTTGTTACTGTAGAAAGTGATATTGCTATTAGGGATACCAGTGAAACTGGCAGT
CCAGCATCTGTCAACAGGGTTTCTGCTAATGTTGGTTCTGAGGAGGAAGAAGCAGCCTTTCTTCGCTCACTTGGGTGGGAAGAAAATGCTAGTGGAGAAGAGGCACTCACAGAGGAGGAAATCAATTCTTTTTACCGGGAGCACATGAGGGCAGTAGCTGCTAATGCTTTTCGCCGAGGCAGCCATAGACATTGGAACCT
GAGATTTGATATGCATGTTGGAAATAATGTGGAGAGCTTTTCTTCTGGTATAGGCTCTTCAGAGTCAGCTTAGATACAGGGTGACACTCATATGGGAAAGAAAAACTAATAGCGTGGCAGGGATTAGATAGATATAAGATTTGGAAAATTTTGGATTGACTGAAAACATGAAAAAAACTGGCTCTTACCCATGTGTTGCT
TCAGAAGGTGCAATCATAAAGAGGTTGGGTAACGAAGTTCCCTTGAGAGCTTTAGATTGTCAACTTGACAAAGTAGATTGCTGTCAAAGTTGGCAAACGACAACTTATTTTGGATGAATAGATAATTTTGCAATGTGTGCATATGAAATATTTGTCACAAGAACAGCACAGAATTATGAAATGCAAAGAACTGTGCTCAT
TAGTAATTTCCATGGTTTTACTCGGTGGTTTTACTCAGAAAGATGTATATGATTCTTGACTTCTGCTGTTCATGCAGAAACGTGGCAGTGAATGTATATTCATGATGCAAAGTGTCCTTTTCCCAGGTATAGTGTTTGTGCCTTTAGCCAATTTGTGATTTTGGTTTCAGAGTAGCAAGATTGGTGGGATGCTTCAACAA
CCTCACTCTGAAATTTAATGGGCTGGGAATCATCTTCTTCGTCTTACTGATTCATTTTTGCTGGGAAGAAAGCTTTGGTCCTCTGCTGGTTAGTCTCTCATTGACACTTAAAACTAGAATATTCCAATTTTTGAGGTATACAAGTTGGAAAGATTGTATTGGGATACGAATCAATTTTGTAATCTTCTGAACTGCGATTT
GTTTTATTTATTCAAAAAGGTATTATTCTTTTTAGTTGACATTTCAGTTCAGGACTTGAATTTGTATTTGAGGATACATACATAATACATTGCCTGCCATATGACCGAAACATGAATATAACAGCAACAGTAACACTGAATATTAATAACGGAAGAAGTGCAGTCAGTGAAGTAATAGAGCGAATTTCTCACATTAAATG
AGTTCCTGGCATCTTGCAGACAAACAGTTTAGATGTCATTCTGAAACATGCGTATCTCTTGCATGGTTTTTAATTAGTAATCTGATTGCATGATATTTTAA

Intron 2 GTATTGATAG ... TGTACTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.96	82	9	1
A2	18.67	61	14	3
A3	15.39	60.5	11	1
A4	7.09	66	5	0
A5	18.55	22	5	0
A6	9.84	46	5	0
A7	8.83	62	6	0
A8	5.59	34	2	0
A9	9.91	45.5	5	0
A10	15.87	69	13	6

>TRINITY_DN109587_c1_g2_i4 from sample A2 / Example spliced isoform

GGAAAATGGAAATGGATATGCAATTGCTCTGTTGAGGTCGAGGGTGAGAGCAGAGGGGGGAGGAATTAGAATTGGAATGGAAAAGAAGTTGAAGACCAACCATCAACGGCGGCACAGGATCCCTAGGGTTTAGTATTCTGGATTGTTGAAGAATGGCAGGCTTTTGACAGTTACATATTCTCCCAACACAGGGCACTGCA
GGTTCTACGTTTCTTTTTGTTTCTCTCCCTCTGTTTTCTTTTTCGCTTGAGCCCCGGGGGCCATTTTATTTATAAAGGCTAGGGTCGTGTCTTCAGAGTGCAGTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCCGAACCTGGGTCTATTTCAAGGGATTTGATTGAATCATGCAGCATAGCTTATTACTAT
TATTGTTGCTACTAAGGTAATTGCCCAATCGCTAATTCAATTTGCGGTTATTGTGCACTGATATCTCAAGAACCCTGCGGGCTGCTGATTATTATCTTTTATGGAAATGGGTTTGTAAAGCGAAATTGCAGAGTTGAGGAACCCAATTTTTTTTCTCACGGAAGACATTGGTGAGGCTGCTTTGGAGTGAACTGTCTGAA
GTCTTTACTTTCCAGCAAGAAGGCTTGAAATTGCAGAGTTGAGGGATAGAAGTGAAAGCAACATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGCTGTTTTGGAAAACGGAGTAAATTTGTTTATTGTAATTTGAGGGATCAGGAAACCAGTTTGCTTGGTGGACTGGATTGGCTCTGCAATGTTCTGTGCTGTGCAT
TTAGAGGATTTTTATTTGCATCTGCTGTCGGAAACAAGAGTGTTGTCGCTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGGCAGTTACATAACTTCAAAATAAGGCACAGGAACCATCATTTTATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGT
TTTCAAAAAGGAGGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCTGGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACA
CCATCAGACAATACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCTGTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACA
TTCGCGTGCCTATACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTGAGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTG
CTGGCAGGTTTGAATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGTGAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATC
ATTCAATCTAACCTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTGCTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCA
AATTGCACCAGGAAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAAAGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTA
TGGTATTCACTCTTTACATTTTATATTTGCTATTGTTCTATGTAGGATTTCTTGCTTTTGTACCATGATTTGAATTGATAAGATAATGTTCATAAAATGCTCTGTTTTTTTTGTTTTTTGTTTTTGTTTTTTTGTTTTTAATTTTGAATGTGTGACACAAAAATTGGTTTA

>TRINITY_DN109587_c1_g2_i3 from sample A2 / Example unspliced isoform:

CGGACGTATGGCATGCGGAACACGGTCACATGCTCCATGGTCACATGGTAAGCTCTCGTAGACGGCAAAAACCCTCTTATCGTTCTCTCTATCCTTCCCGAGTTCAAGTTTTCTGTCCATGTTCATCAGCAGCGGAGAAGCAGAGGAAGAAGCAGCCGCCCGATAAATCTGGCGAGCGAAAATATTTGTGAGAGCGTCCG
AGGTTTCTGTTTGCCTTGAATAGCAATTGCTGTAGGGTTTACTGGCACACTATTATTCGGTGGCCGGGGTTTTCTGCGGGGCGAATGGTGTTTTACAGCGATAGGACAGAGGGCAGGTGTGGTGGTTTTCGTGAAGTCGACTTGGAATTCTTAGGGTTCGGGAGAGAAAATTGTGCAGGGTTAGGCGGATGGAACAGGTC
AGTTTGATTTAATCCCAGTGGTCAAACTCCAATGTGGCAGGGCGGAATTCTGAGACAGGGCTGTGGCAGAACGTGCTTAGCTGAGTTAGATGCACGTTTCGGCAGGAGCCCTAGGGTTTAATATTCTGGATTGTTGAAGAATGGCAGGCTTTTGACAGTTACATATTCTCCCAACACAGGGCACTGCAGGTTCTACGTTT
CTTTTTGTTTCTCTCCCTCTGTTTTCTTTTTCGCTTGAGCCCCGGGGGCCATTTTATTTATAAAGGCTAGGGTCGTGTCTTCAGAGTGCAGTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCCGAACCTGGGTCTATTTCAAGGGATTTGATTGAATCATGCAGCATAGCTTATTACTATTATTGTTGCTAC
TAAGGTAATTGCCCAATCGCTAATTCAATTTGCGGTTATTGTGCACTGATATCTCAAGAACCCTGCGGGCTGCTGATTATTATCTTTTATGGAAATGGGTTTGTAAAGCGAAATTGCAGAGTTGAGGAACCCAATTTTTTTTCTCACGGAAGACATTGGTGAGGCTGCTTTGGAGTGAACTGTCTGAAGTCTTTACTTTC
CAGCAAGAAGGCTTGAAATTGCAGAGTTGAGGGATAGAAGTGAAAGCAACATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGCTGTTTTGGAAAACGGAGTAAATTTGTTTATTGTAATTTGAGGGATCAGGAAACCAGTTTGCTTGGTGGACTGGATTGGCTCTGCAATGTTCTGTGCTGTGCATTTAGAGGATTTT
TATTTGCATCTGCTGTCGGAAACAAGAGTGTTGTCGCTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGGCAGTTACATAACTTCAAAATAAGGCACAGGAACCATCATTTTATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGTTTTCAAAAAGGA
GGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCTGGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACACCATCAGACAAT
ACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCTGTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACATTCGCGTGCCTA
TACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTGAGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTGCTGGCAGGTTTG
AATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGTGAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATCATTCAATCTAAC
CTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTGCTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCAAATTGCACCAGG
AAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGgtattgatagctctatttctgtttctttgacccgtaagctgtgttgcataaactttggtagttgctgaagagatttttgactcttttgttgtactgtagT
TATCTACTGAGGTTCAAAGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTATGG

Intron 3 GTTCTACGTT ... CAAAGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.12	24	33	65
A2	71.07	23	77	36
A3	73.33	22	71	50
A4	63.64	20	31	39
A5	63.22	16	29	26
A6	58.63	127	266	94
A7	60.66	155	385	93
A8	54.63	174	317	102
A9	55.46	210	408	115
A10	60.19	166	368	134

>TRINITY_DN44092_c0_g1_i1 from sample A6 / Example spliced isoform

CCTAAAGAGAGGGGGGGAGGGAAAATGGAAATGCATATGCAATTGCTCTGTTGAGGTCAAGGGTGAGAGCAGAGGGGGGAGGAATTAGAATTGGGATGGAAAAGAAGTTGAAGACTAACCACCAACTGCGGCACAGGAGACCTAGGGTTTAGTATTTTGGATTGTTGAAGAATGGCAGCCTTTTGACAGTTACATATGCT
CCCAACACAGGGAACTGCAGTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCTGAACCTGGGTCTATTTCAACGGATTTGATTGAATCATGCAGCATAGCTTATTACTATTGTTGTTTCTACTAAGCGAAATTGCAGAGTAGAGGAACCCGATTTTTTTTTCTCACAGAAGGCATTAGGGAGGCTGCTTTGGA
GTGAACTGCTTGAAGTCTTTACTTTCCAGCAAGGAGGCCCATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGTTGTTTTGAAAACGGAGTAAATTTGTTTATTGTAATTTGAGGGATCAGGAAACCAGTTTGCTTGGTGGACTGGATTGGCTCTGCAATGTTCTGTGCTGTGCATACTTGGTGGACTGGATTGGCTCT
ACAATGTTCTGTGCTATGCATCTAGAGGATTTTTATTTGCATCTACTGTCGGAAACAAGAGTGTTGTTATTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGGCAATTACATAACTTCAAAATAAGGGACAGGAACTATCATTTTATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAG
AGGAGTTTTATTTGCATCTGTTTTCAAAAAGGAGGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCTGGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGT
CATCATATTTTTTCTGCTACACCATCAGACAATACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCTGTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATAC
TGACAGAGATCCATTTGGACATTCGCGTGCCTATACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTGAGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAA
GGTGGTCTAATCCAGGACCTGCTGGCAGGTTTGAATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGTGAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGA
GCTGAAGAGAAGCAATGTATCATTCAATCTAACCTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTGCTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGG
CCCATCTACAGCAAGTCAGCAAATTGCACCAGGAAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAAAGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGA
CTCCGTCGATGCCAAAAACTATGGGTCTTAGCCCTTCTGAAAAGTCAAAGGCAAAGTCATTGTGGTCAATTGATACAACTGCTTCTGCTGTTAAAGGTATTCAATCAGTAGGATCATCCCATTCTATGAACTCTCCCAAAGTTACTCAGGGTGGGAAGCTTCTAGTTCTCAAGCCAGCAAGGGATTCGGGATCTTCTGTG
CCTGTCAAACCTAATAATGGCAGTCCAGTGAAGGGAGGCAATACCAGTAGCAGCAGCTCATTTGGTGTATCAGTCAGAGGTGCTATTGATGTCAGTGGAAATGCTAGTCTGGTGGGTTTTAGGAAGCAGAAACCCCTCGTTGACCCCAGAGCAGTATTCTCATCTGGAGTGGATAGTGGGCTAGGGACACGGGGAAAGGA
GGCCAACTTATTGATTGAGGAAAAAAAGTCTGTGTCACAAGCACAAAATCGGAGTGATTTTTTCAATGCATTGAGGAGGAAAGCTTGTGAGAGCCCCAATTCCACTGCAACTCAGGACAAGTCTAATACATCAACAGTGCATAAAGCTGATCGAGAAAATGAGAATATGCTCCTGAGCACCAAGGATTTATTAGAACTAA
AGGATAAGTGTTCTGTTGAGAATGTAGGTGAGAAATCTGATACTGTTCCTTCAATGGTTGAAACAGAAGCCACTGAGAAAAATAAAACTATCTTGAATGGTTTTGTTACTGTAGAAAGTGATATTGCTATTAGGGATACCAGTGAAACTGGCAGTCCAGCATCTGTCAACAGGGTTTCTGCTAATGTTGGTTCTGAGGAG
GAAGAAGCAGCCTTTCTTCGCTCACTTGGGTGGGAAGAAAATGCTAGTGGAGAAGAGGCACTCACAGAGGAGGAAATCAATTCTTTTTACCGGGAGCACATGAGGGCAGTAGCTGCTAATGCTTTTCGCCGAGGCAGCCATAGACATTGGAACCTGAGATTTGATATGCATGTTGGAAATAATGTGGAGAGCTTTTCTTC
TGGTATAGGCTCTTCAGAGTCAGCTTAGATACAGGGTGACACTCATATGGGAAAGAAAAACTAATAGCGTGGCAGGGATTAGATAGATATAAGATTTGGAAAATTTTGGATTGACTGAAAACATGAAAAAAACTGGCTCTTACCCATGTGTTGCTTCAGAAGGTGCAATCATAAAGAGGTTGGGTAACGAAGTTCCCTTG
AGAGCTTTAGATTGTCAACTTGACAAAGTAGATTGCTGTCAAAGTTGGCAAACGACAACTTATTTTGGATGAATAGATAATTTTGCAATGTGTGCATATGAAATATTTGTCACAAGAACAGCACAGAATTATGAAATGCAAAGAACTGTGCTCATTAGTAATTTCCATGGTTTTACTCGGTGGTTTTACTCAGAAAGATG
TATATGATTCTTGACTTCTGCTGTTCATGCAGAAACGTGGCAGTGAATGTATATTCATGATGCAAAGTGTCCTTTTCCCAGGTATAGTGTTTGTGCCTTTAGCCAATTTGTGATTTTGGTTTCAGAGTAGCAAGATTGGTGGGATGCTTCAACAACCTCACTCTGAAATTTAATGGGCTGGGAATCATCTTCTTCGTCTT
ACTGATTCATTTTTGCTGGGAAGAAAGCTTTGGTCCTCTGCTGGTTAGTCTCTCATTGACACTTAAAACTAGAATATTCCAATTTTTGAGGTATACAAGTTGGAAAGATTGTATTGGGATACGAATCAATTTTGTAATCTTCTGAACTGCGATTTGTTTTATTTATTCAAAAAGGTATTATTCTTTTTAGTTGACATTTC
AGTTCAGGACTTGAATTTGTATTTGAGGATACATACATAATACATTGCCTGCCATATGACCGAAACATGAATATAACAGCAACAGTAACACTGAATATTAATAACGGAAGAAGTGCAGTCAGTGAAGTAATAGAGCGAATTTCTCACATTAAATGAGTTCCTGGCATCTTGCAGACAAACAGTTTAGATGTCATTCTGAA
ACATGCGTATCTCTTGCATGGTTTTTAATTAGTAATCTGATTGCATGATATTTTAACTGCCTTCTAAAAAATCCATGATTTAAATTTCTGTCAATATACTGCTTTTCTTGATGGATATGGTGAATTCTTATAGACTGTCCTCCATGCTATTTTAACG

>TRINITY_DN44092_c0_g1_i10 from sample A6 / Example unspliced isoform:

CCTAAAGAGAGGGGGGGAGGGAAAATGGAAATGCATATGCAATTGCTCTGTTGAGGTCAAGGGTGAGAGCAGAGGGGGGAGGAATTAGAATTGGGATGGAAAAGAAGTTGAAGACTAACCACCAACTGCGGCACAGGAGACCTAGGGTTTAGTATTTTGGATTGTTGAAGAATGGCAGCCTTTTGACAGTTACATATGCT
CCCAACACAGGGAACTGCAGgttctacgtttctttttatttctctccctcccctctgttttctttttcggttgagccccggggggcattttatttacaaaggccagggccgtgtcttcctgttttctttctcggttgaaccccaggggccatttaatttacaaaggctagaaccgtgtcttcaaagtgcagTTTGTCTAG
GGTTTTTAGAATGCGGCAGTTCCGGGGCCCTGAACCTGGGTCTATTTCAACGGATTTGATTGAATCATGCAGCATAGCTTATTACTATTGTTGTTTCTACTAAGCGAAATTGCAGAGTAGAGGAACCCGATTTTTTTTTCTCACAGAAGGCATTAGGGAGGCTGCTTTGGAGTGAACTGCTTGAAGTCTTTACTTTCCAG
CAAGGAGGCCCATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGTTGTTTTGAAAACGGAGTAAATTTGTTTATTGTAATTTGAGGGATCAGGAAACCAGTTTGCTTGGTGGACTGGATTGGCTCTGCAATGTTCTGTGCTGTGCATACTTGGTGGACTGGATTGGCTCTACAATGTTCTGTGCTATGCATCTAGAGGA
TTTTTATTTGCATCTACTGTCGGAAACAAGAGTGTTGTTATTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGGCAATTACATAACTTCAAAATAAGGGACAGGAACTATCATTTTATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGTTTTCAAAA
AGGAGGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCTGGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACACCATCAGA
CAATACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCTGTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACATTCGCGTG
CCTATACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTGAGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTGCTGGCAGG
TTTGAATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGTGAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATCATTCAATC
TAACCTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTGCTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCAAATTGCAC
CAGGAAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAAAGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTATGGGTCTT
AGCCCTTCTGAAAAGTCAAAGGCAAAGTCATTGTGGTCAATTGATACAACTGCTTCTGCTGTTAAAGGTATTCAATCAGTAGGATCATCCCATTCTATGAACTCTCCCAAAGTTACTCAGGGTGGGAAGCTTCTAGTTCTCAAGCCAGCAAGGGATTCGGGATCTTCTGTGCCTGTCAAACCTAATAATGGCAGTCCAGT
GAAGGGAGGCAATACCAGTAGCAGCAGCTCATTTGGTGTATCAGTCAGAGGTGCTATTGATGTCAGTGGAAATGCTAGTCTGGTGGGTTTTAGGAAGCAGAAACCCCTCGTTGACCCCAGAGCAGTATTCTCATCTGGAGTGGATAGTGGGCTAGGGACACGGGGAAAGGAGGCCAACTTATTGATTGAGGAAAAAAAGT
CTGTGTCACAAGCACAAAATCGGAGTGATTTTTTCAATGCATTGAGGAGGAAAGCTTGTGAGAGCCCCAATTCCACTGCAACTCAGGACAAGTCTAATACATCAACAGTGCATAAAGCTGATCGAGAAAATGAGAATATGCTCCTGAGCACCAAGGATTTATTAGAACTAAAGGATAAGTGTTCTGTTGAGAATGTAGGT
GAGAAATCTGATACTGTTCCTTCAATGGTTGAAACAGAAGCCACTGAGAAAAATAAAACTATCTTGAATGGTTTTGTTACTGTAGAAAGTGATATTGCTATTAGGGATACCAGTGAAACTGGCAGTCCAGCATCTGTCAACAGGGTTTCTGCTAATGTTGGTTCTGAGGAGGAAGAAGCAGCCTTTCTTCGCTCACTTGG
GTGGGAAGAAAATGCTAGTGGAGAAGAGGCACTCACAGAGGAGGAAATCAATTCTTTTTACCGGGAGCACATGAGGGCAGTAGCTGCTAATGCTTTTCGCCGAGGCAGCCATAGACATTGGAACCTGAGATTTGATATGCATGTTGGAAATAATGTGGAGAGCTTTTCTTCTGGTATAGGCTCTTCAGAGTCAGCTTAGA
TACAGGGTGACACTCATATGGGAAAGAAAAACTAATAGCGTGGCAGGGATTAGATAGATATAAGATTTGGAAAATTTTGGATTGACTGAAAACATGAAAAAAACTGGCTCTTACCCATGTGTTGCTTCAGAAGGTGCAATCATAAAGAGGTTGGGTAACGAAGTTCCCTTGAGAGCTTTAGATTGTCAACTTGACAAAGT
AGATTGCTGTCAAAGTTGGCAAACGACAACTTATTTTGGATGAATAGATAATTTTGCAATGTGTGCATATGAAATATTTGTCACAAGAACAGCACAGAATTATGAAATGCAAAGAACTGTGCTCATTAGTAATTTCCATGGTTTTACTCGGTGGTTTTACTCAGAAAGATGTATATGATTCTTGACTTCTGCTGTTCATG
CAGAAACGTGGCAGTGAATGTATATTCATGATGCAAAGTGTCCTTTTCCCAGGTATAGTGTTTGTGCCTTTAGCCAATTTGTGATTTTGGTTTCAGAGTAGCAAGATTGGTGGGATGCTTCAACAACCTCACTCTGAAATTTAATGGGCTGGGAATCATCTTCTTCGTCTTACTGATTCATTTTTGCTGGGAAGAAAGCT
TTGGTCCTCTGCTGGTTAGTCTCTCATTGACACTTAAAACTAGAATATTCCAATTTTTGAGGTATACAAGTTGGAAAGATTGTATTGGGATACGAATCAATTTTGTAATCTTCTGAACTGCGATTTGTTTTATTTATTCAAAAAGGTATTATTCTTTTTAGTTGACATTTCAGTTCAGGACTTGAATTTGTATTTGAGGA
TACATACATAATACATTGCCTGCCATATGACCGAAACATGAATATAACAGCAACAGTAACACTGAATATTAATAACGGAAGAAGTGCAGTCAGTGAAGTAATAGAGCGAATTTCTCACATTAAATGAGTTCCTGGCATCTTGCAGACAAACAGTTTAGATGTCATTCTGAAACATGCGTATCTCTTGCATGGTTTTTAAT
TAGTAATCTGATTGCATGATATTTTAACTGCCTTCTAAAAAATCCATGATTTAAATTTCTGTCAATATACTGCTTTTCTTGATGGATATGGTGAATTCTTATAGACTGTCCTCCATGCTATTTTAACG

Intron 4 GTTCGTATTC ... ACTTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	14.29	60	10	0
A2	22.08	60	17	0
A3	17.54	47	10	0
A4	16.09	73	14	0
A5	33.33	30	15	2
A6	18.91	163	38	0
A7	18.64	192	44	0
A8	20.41	195	50	0
A9	17.77	162	35	0
A10	20.22	146	37	0

>TRINITY_DN103288_c0_g2_i4 from sample A5 / Example spliced isoform

TCGACTTGGAATTCTTAGGGTTCAAGTTTTCTGTCCATGTTCATCAGCAGCGGAGAAGCAGAGGAAGAAGCAGCCGCCCGATAAATCTGGCGAGCGAAAATATTTGTGAGAGCGTCCGAGGTTTCTGTTTGCCTTGAATAGCAATTGCTGTAGGGTTTACTGGCACACTATTATTCGGTGGCCGGGGTTTTCTGCGGGGC
GAATGGTGTTTTACAGCGATAGGACAGAGGGCAGGTGTGGTGGTTTTCGTGAAGTCGACTTGGAATTCTTAGGGTTCGGGAGAGAAAATTGTGCAGGGTTAGGCGGATGGAACAGGTCAGTTTGATTTAATCCCAGTGGTCAAACTCCAATGTGGCAGGGCGGAATTCTGAGACAGGGCTGTGGCAGAACGTGCTTAGCT
GAGTTAGATGCACGTTTCGGCAGGAGCCCTAGGGTTTAATATTCTGGATTGTTGAAGAATGGCAGGCTTTTGACAGTTACATATTCTCCCAACACAGGGCACTGCAGGTTCTACGTTTCTTTTTGTTTCTCTCCCTCTGTTTTCTTTTTCGCTTGAGCCCCGGGGGCCATTTTATTTATAAAGGCTAGGGTCGTGTCTTC
AGAGTGCAGTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCCGAACCTGGGTCTATTTCAAGGGATTTGATTGAATCATGCAGCATAGCTTATTACTATTATTGTTGCTACTAAGGTAATTGCCCAATCGCTAATTCAATTTGCGGTTATTGTGCACTGATATCTCAAGAACCCTGCGGGCTGCTGATTATTA
TCTTTTATGGAAATGGGTTTGTAAAGCGAAATTGCAGAGTTGAGGAACCCAATTTTTTTTCTCACGGAAGACATTGGTGAGGCTGCTTTGGAGTGAACTGTCTGAAGTCTTTACTTTCCAGCAAGAAGGCTCATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGCTGTTTTGGAAAACGGAGTAAATTTGTTTATTGT
AATTTGAGGGATCAGGAAACCAGTTTGCTTGGTGGACTGGATTGGCTCTGCAATGTTCTGTGCTGTGCATTTAGAGGATTTTTATTTGCATCTGCTGTCGGAAACAAGAGTGTTGTCGCTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGGCAGTTACATAACTTCAAAATAAGGCACAGGAACCATCATTT
TATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGTTTTCAAAAAGGAGGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCT
GGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACACCATCAGACAATACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCT
GTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACATTCGCGTGCCTATACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTG
AGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTGCTGGCAGGTTTGAATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGT
GAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATCATTCAATCTAACCTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTG
CTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCAAATTGCACCAGGAAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAA
AGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTATGGGTCTTAGCCCTTCTGAAAAGTCAAAGGCAAAGTCATTGTGGTCAATTGATACAACTGCTTCTGCTGTTAAAGGTATTCAATCAGTAGGATCATCCCATTCTATGAACTCTCCCAAAGTTACTCAGGG
TGGGAAGCTTCTAGTTCTCAAGCCAGCAAGGGATTCGGGATCTTCTGTGCCTGTCAAACCTAATAATGGCAGTCCAGTGAAGGGAGGCAATACCAGTAGCAGCAGCTCATTTGGTGTATCAGTCAGAGGTGCTATTGATGTCAGTGGAAATGCTAGTCTGGTGGGTTTTAGGAAGCAGAAACCCCTCGTTGACCCCAGAG
CAGTATTCTCATCTGGAGTGGATAGTGGGCTAGGGACACGGGGAAAGGAGGCCAACTTATTGATTGAGGAAAAAAAGTCTGTGTCACAAGCACAAAATCGGAGTGATTTTTTCAATGCATTGAGGAGGAAAGCTTGTGAGAGCCCCAATTCCACTGCAACTCAGGACAAGTCTAATACATCAACAGTGCATAAAGCTGAT
CGAGAAAATGAGAATATGCTCCTGAGCACCAAGGATTTATTAGAACTAAAGGATAAGTGTTCTGTTGAGAATGTAGGTGAGAAATCTGATACTGTTCCTTCAATGGTTGAAACAGAAGCCACTGAGAAAAATAAAACTATCTTGAATGGTTTTGTTACTGTAGAAAGTGATATTGCTATTAGGGATACCAGTGAAACTGG
CAGTCCAGCATCTGTCAACAGGGTTTCTGCTAATGTTGGTTCTGAGGAGGAAGAAGCAGCCTTTCTTCGCTCACTTGGGTGGGAAGAAAATGCTAGTGGAGAAGAGGCACTCACAGAGGAGGAAATCAATTCTTTTTACCGGGAGCACATGAGGGCAGTAGCTGCTAATGCTTTTCGCCGAGGCAGCCATAGACATTGGA
ACCTGAGATTTGATATGCATGTTGGAAATAATGTGGAGAGCTTTTCTTCTGGTATAGGCTCTTCAGAGTCAGCTTAGATACAGGGTGACACTCATATGGGAAAGAAAAACTAATAGCGTGGCAGGGATTAGATAGATATAAGATTTGGAAAATTTTGGATTGACTGAAAACATGAAAAAAACTGGCTCTTACCCATGTGT
TGCTTCAGAAGGTGCAATCATAAAGAGGTTGGGTAACGAAGTTCCCTTGAGAGCTTTAGATTGTCAACTTGACAAAGTAGATTGCTGTCAAAGTTGGCAAACGACAACTTATTTTGGATGAATAGATAATTTTGCAATGTGTGCATATGAAATATTTGTCACAAGAACAGCACAGAATTATGAAATGCAAAGAACTGTGC
TCATTAGTAATTTCCATGGTTTTACTCGGTGGTTTTACTCAGAAAGATGTATATGATTCTTGACTTCTGCTGTTCATGCAGAAACGTGGCAGTGAATGTATATTCATGATGCAAAGTGTCCTTTTCCCAGGTATAGTGTTTGTGCCTTTAGCCAATTTGTGATTTTGGTTTCAGAGTAGCAAGATTGGTGGGATGCTTCA
ACAACCTCACTCTGAAATTTAATGGGCTGGGAATCATCTTCTTCGTCTTACTGATTCATTTTTGCTGGGAAGAAAGCTTTGGTCCTCTGCTGGTTAGTCTCTCATTGACACTTAAAACTAGAATATTCCAATTTTTGAGGTATACAAGTTGGAAAGATTGTATTGGGATACGAATCAATTTTGTAATCTTCTGAACTGCG
ATTTGTTTTATTTATTCAAAAAGGTATTATTCTTTTTAGTTGACATTTCAGTTCAGGACTTGAATTTGTATTTGAGGATACATACATAATACATTGCCTGCCATATGACCGAAACATGAATATAACAGCAACAGTAACACTGAATATTAATAACGGAAGAAGTGCAGTCAGTGAA

>TRINITY_DN103288_c0_g2_i3 from sample A5 / Example unspliced isoform:

TCGACTTGGAATTCTTAGGGTTCAAGTTTTCTGTCCATGTTCATCAGCAGCGGAGAAGCAGAGGAAGAAGCAGCCGCCCGATAAATCTGGCGAGCGAAAATATTTGTGAGAGCGTCCGAGGTTTCTGTTTGCCTTGAATAGCAATTGCTGTAGGGTTTACTGGCACACTATTATTCGGTGGCCGGGGTTTTCTGCGGGGC
GAATGGTGTTTTACAGCGATAGGACAGAGGGCAGGTGTGGTGGTTTTCGTGAAGTCGACTTGGAATTCTTAGGGTTCGGGAGAGAAAATTGTGCAGGGTTAGGCGGATGGAACAGGTCAGTTTGATTTAATCCCAGTGGTCAAACTCCAATGTGGCAGGGCGGAATTCTGAGACAGGGCTGTGGCAGAACGTGCTTAGCT
GAGTTAGATGCACGTTTCGGCAGGAGCCCTAGGGTTTAATATTCTGGATTGTTGAAGAATGGCAGGCTTTTGACAGTTACATATTCTCCCAACACAGGGCACTGCAGGTTCTACGTTTCTTTTTGTTTCTCTCCCTCTGTTTTCTTTTTCGCTTGAGCCCCGGGGGCCATTTTATTTATAAAGGCTAGGGTCGTGTCTTC
AGAGTGCAGTTTGTCTAGGGTTTTTAGAATGCGGCAGTTCCGGGGCCCCGAACCTGGGTCTATTTCAAGGGATTTGATTGAATCATGCAGCATAGCTTATTACTATTATTGTTGCTACTAAGGTAATTGCCCAATCGCTAATTCAATTTGCGGTTATTGTGCACTGATATCTCAAGAACCCTGCGGGCTGCTGATTATTA
TCTTTTATGGAAATGGGTTTGTAAAGCGAAATTGCAGAGTTGAGGAACCCAATTTTTTTTCTCACGGAAGACATTGGTGAGGCTGCTTTGGAGTGAACTGTCTGAAGTCTTTACTTTCCAGCAAGAAGGCTCATAGTTTTGAGGTTTTGAGTTGAGACTGTCTTGGCTGTTTTGGAAAACGGAGTAAATTTGTTTATTGT
AATTTGAGGGATCAGGAAACCAGTTTGCTTGGTGGACTGGATTGGCTCTGCAATGTTCTGTGCTGTGCATTTAGAGGATTTTTATTTGCATCTGCTGTCGGAAACAAGAGTGTTGTCGCTGTACCGAGTTTCGGTCTGATTCAAATTAAAAGTGTTCGGCAGTTACATAACTTCAAAATAAGGCACAGGAACCATCATTT
TATTTAGTGGACTGACTTTGCTCTGCAATGTTATGCGTTGTACATCTAGAGGAGTTTTATTTGCATCTGTTTTCAAAAAGGAGGGTGCTGTCGCGGCACTGAGAGTTTCGGTCTGATTCAAATTAAAAGGGTTAATTAGGTACAGAAAAAATAAATTTATCATGGTCTATAGCAGGTATGGATAGAAGTGAGCCTTCTCT
GGTTCCAGAATGGCTTAAAGGACTTAGTGGCGGGAGTGCAAGCAGTGGTCATCATATTTTTTCTGCTACACCATCAGACAATACCGACGCTCCATTGGCATCTAGACATAGATCTCTTCAATTACAATCATCCGGAGCAACTCGTGGTGACTACGGCTCACCCCGATATTCAGGGTCATCGGAGAGATCATTTTTGCCCT
GTCGTAGGAGCCTAAATAATAACAGTGGGGGTAGTGATCGTACAAATACTGACAGAGATCCATTTGGACATTCGCGTGCCTATACTAGTTTTCGTCGCAGTTCTTCTCTCCGGGTGTATGAGTCTCTAGAACATGAGAAAGATTGGGAATGGGGTCGAGAATGGGAAGCTCATGACAGAGAAAGGTCTCTGGGAGGAGTG
AGTGGAAATGATCGAAACAATGGCCGTGAACTATCTGATACTGCAAAAAGGTGGTCTAATCCAGGACCTGCTGGCAGGTTTGAATCTCTAAGGCGGTCCCAATCTGTAATATCAAATAGGATATCAGACAATGGAGAAAAGAAAATTGGAAATGAGACACGAACCATTACAGCTGCTCCTCTTGTACGTGGTGGTATTGT
GAGTAACATGCAGAAAGCTGCCTTTGAAAAAGATTTTCCCTCTCTTGGAGCTGAAGAGAAGCAATGTATCATTCAATCTAACCTGGCAAATGTTGCTGGGATGAACTCAAGTTCAAGCCCAATTTGGCAAGGACAAGGGTCCATTGGAAGGCCAGGCACACCAGTAAGTGGGGTTTCTTCTATTGATGGATGGAAATCTG
CTTTAGCAGATGCTCCGGTTCTAAATGGGGTTGTATCACTGGGCAATGGCCCATCTACAGCAAGTCAGCAAATTGCACCAGGAAGCTTAGTTTCATCCAAAGGCCATGGTCTTGCTTCTACAAGTGGACTGAATATGGCTGAAGCATTGGTGCTAAACCCCCCACGACCTCGCACACCTCAGTTATCTACTGAGGTTCAA
AGGATGGAGGTGTTGGCTCTGAAACAGTCGCGACAACTAATACCTGTGACTCCGTCGATGCCAAAAACTATGGGTCTTAGCCCTTCTGAAAAGTCAAAGGCAAAGTCATTGTGGTCAATTGATACAACTGCTTCTGCTGTTAAAGGTATTCAATCAGTAGGATCATCCCATTCTATGAACTCTCCCAAAGTTACTCAGGG
TGGGAAGCTTCTAGTTCTCAAGCCAGCAAGGGATTCGGGATCTTCTGTGCCTGTCAAACCTAATAATGGCAGTCCAGTGAAGGGAGGCAATACCAGTAGCAGCAGCTCATTTGGTGTATCAGTCAGAGGTGCTATTGATGTCAGTGGAAATGCTAGTCTGGTGGGTTTTAGGAAGCAGAAACCCCTCGTTGACCCCAGAG
CAGTATTCTCATCTGGAGTGGATAGTGGGCTAGGGACACGGGGAAAGGAGGCCAACTTATTGATTGAGGAAAAAAAGTCTGTGTCACAAGCACAAAATCGGAGTGATTTTTTCAATGCATTGAGGAGGAAAGCTTGTGAGAGCCCCAATTCCACTGCAACTCAGGACAAGTCTAATACATCAACAGTGCATAAAGCTGAT
CGAGAAAATGAGAATATGCTCCTGAGCACCAAGGATTTATTAGAACTAAAGGATAAGTGTTCTGTTGAGAATGTAGGTGAGAAATCTGATACTGTTCCTTCAATGGTTGAAACAGAAGCCACTGAGAAAAATAAAACTATCTTGAATGGTTTTGTTACTGTAGAAAGTGATATTGCTATTAGGGATACCAGTGAAACTGG
CAGTCCAGCATCTGTCAACAGGGTTTCTGCTAATGTTGGTTCTGAGGAGGAAGAAGCAGCCTTTCTTCGCTCACTTGGGTGGGAAGAAAATGCTAGTGGAGAAGAGGCACTCACAGAGGAGGAAATCAATTCTTTTTACCGGGAGgttcgtattctcatttgttgaatgttaatagctgcttgtgttttgaaatgaggct
ccagcaattttgatttaagttgtcttattttaacagcatttctgagagttgtcttattttcacttttgcagCACATGAGGGC