sp|Q8LHP0|ELOF1_ORYSJ ----> Transcription elongation factor 1 homolog

Intron 1 GTCAGTGCGG ... GAATTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	3.8	328.4	10.4	12
A2	4.96	314.8	1	15.5
A3	3.94	321.4	5.8	12.5
A4	3.14	200.8	1.9	6
A5	2.63	155.6	0	4
A6	3.03	3051.2	26.7	90.8
A7	2.83	2777.3	6.5	77.5
A8	3.28	2533.4	17.2	83.3
A9	2.8	2754.3	11.2	76
A10	2.27	2306.8	21.4	51.4

>TRINITY_DN114405_c0_g1_i2 from sample A2 / Example spliced isoform

GTGACTATAGATTGCACTCACGCATTTGGCTTCGGTTTTTATTTTGCGTGGATTTGCAGTTTCACTAGCTCGGGGAATTCATTCACAGGTTCCAAGGCGGGAGAATTACTGTGTAATTTGGAGGATTTTAAGGGCAGATATGGGGAAGAGGAAGTCATCGAAGAAGCCGGAGAGCAAAAGAAAGCAGGAAAAACTCAGCA
CAGTCTTCAGCTGCCCTTTCTGCAACCACGACAGCAGCGTGGAGTGCCGCATGGATCGGAAAGATCTTATTGGGGAGGCCTCATGCAGGATATGCCAGGAGAGTTTCAGCACCTCAATAAATGCATTGAGCGAGCCCATTGACGTGTATTCGGAATGGATTGATGAATGTGAACGTGTAAATAATTCTGGATAAAATGGT
TGCATTTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTAACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCGCTTTACCCATAATGTATATTATAGGAAGCCATTTGAACAAATTCACAATATTCTACTCTGGCCTGTCATGTCCTTGGATCATGTTTGACAAATAACTG
TGATGTTCAGTCACTTCATATATCTACTATATGGATGAGCAGTGTATATGACAGTAACATAGTTTGTTATTTGAAACTAAAATTGGAATATAGATATTTTGTATATCTTCTGGCCAAATCTGCTATGGATGAAGAATTTATTTATTTTCATTTTTTGCC

>TRINITY_DN114405_c0_g1_i6 from sample A2 / Example unspliced isoform:

GTTTCAGCACCTCAATAAATGgtcagtgcagtttttggtgttttcctaatatactttttatgtatgattatgttttttcctgagtgccttttccgcttttgagcccttactattatctcttggattaatttgaattgacagCATTGAGCGAGCCCATTGACGTGTATTCGGAATGGATTGATGAATGTGAACGTGTAAAT
AATTCTGGATAAAATGGTTGCATTTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTAACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCGCTTTACCCATAATGTATATTATAGGAAGCCATTTGAACAAATTCACAATATTCTACTCTGGCCTGTCATGTCCTTGGATC
ATGTTTGACAAATAACTGTGATGTTCAGTCACTTCATATATCTACTATATGGATGAGCAGTGTATATGACAGTAACATAGTTTGTTATTTGAAACTAAAATTGGAATATAGATATTTTGTATATCTTCTGGCCAAATCTGCTATGGATGAAGAATTTATTTATTTTCATTTTTTGCC

Intron 2 GTCAGTGCAG ... GAATTGACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	2.19	383.6	9	7.4
A2	1.4	369.1	1	9.1
A3	1.22	379.1	5.1	3.9
A4	0.36	236	0.7	1
A5	0.35	182.7	0	1.1
A6	0.44	3643.4	23.6	6.9
A7	0.18	3305.7	5.1	6.1
A8	0.35	3005.4	14.3	6.1
A9	0.23	3287.6	9	5.1
A10	0.5	2711.4	17.1	8.4

>TRINITY_DN121146_c5_g3_i5 from sample A1 / Example spliced isoform

TATAAGCTGCACTCACGCATTTGGTGTCATATCACTTCGGTTTTTGTTTGCGTGGATTTGCAGTTTTACTCGCACGGGGAATTCACTCGCAGGCGGGAGAATTACTGTGTAATTTGGAGCGTTTTAAGGGCAGATATGGGGAAGAGGAAGTCATCGAAGAAGCCGGAGAGCAAGAGAAAGCAGGAAAAGCTCAGCACAGT
GTTCAGCTGCCCTTTTTGCAACCACGATAGCAGCGTGGAGTGCCGCATGGATCGGAAAGATCTTATTGGGGAGGCCTCATGCAGGATATGCCAGGAGAGTTTCAGCACCTCAATAAATGCATTGAGCGAGCCCATTGACGTGTATTCCGAATGGATTGATGAATGCGAACGTGTAAATAATTCTGGATAAAATGGTTGCA
TATGCATACTTAGATAACATATTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTAACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCACGTTATTCATAATGTATATTATAGGAAGCCATTTGAACAAATTCACTATATTCTACTCTGGCCTGTCGTGTCCTTGGATCAT
GTTTGAAAAATAACTGTGATGTTCAGTCACTTCATATATCTACTATATGGATGAGCTGTGTATGCGACAGTAACATAGTTTGTTATTTGAAACTAAAATTGGAATATAGATATTTTGTATATCTTCTGGCCAAATCTGCTATGGATGAAG

>TRINITY_DN121146_c5_g3_i2 from sample A1 / Example unspliced isoform:

ATTAAACAATGCTATGACAATGGATGGTGATGACTGTTAATATTGATGCAAATTTCCGTGAATAGGACGGATGGTGCATGGACACTAATTCAGAGGTGTACCTGCCTAGTTAAATATCTCCCCTTTGATATTCTTATTTTAACATGCATTTAATGTCGAGCCATTGAAATTGGCCATGGATCGGTGGTTGAACTCAGATA
TTTGATTAACCATTTTGCCTTTGACATCTTAGTGTCATTTTCTTTTGTGTAAGTCAGGTCGAATCTTTGAGAAAGATTTTGAAGTAATTTGGGTATTTTTCTTTTCTTGTATCTTTTGGTTTTCCAGAAAGTATGGTTAGCTATATGAGCCTTAGGCAGTTTGACAATTTTCCAGCTCTGACATTTCTATTCATTCAGGG
ATCGGAAAGATCTTATTGGGGAGGCCTCATGCAGGATATGCCAGGAGAGTTTCAGCACCTCAATAAATGgtcagtgcagtttttggtgttttcctaatatactttttatgtatgattatgttttttcatgagtggtttttccgcttttgagcccttactgaacttacaattatctcttggattaatttgaattgacagCA
TTGAGCGAGCCCATTGATGTGTAAGCTTCCTGAACCATATATTTTAGTGTTTGGGTTTTGACCATTTCCTTTTTAGAAGTGTAATTAGACAGCTGAATAGTTACTTGCAGAAGAGGAATTTTATACACGGAGCAATAAATGGCTATATGGATGACGTGGCCATTTGAATGTCTGAATATGTTTGCAGGTATTCCGAATGG
ATTGATGAATGCGAACGTGTAAATAATTCTGGATAAAATGGTTGCATATGCATACTTAGATAACATATTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTAACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCACGTTATTCATAATGTATATTATAGGAAGCCATTTGA
ACAAATTCACTATATTCTACTCTGGCCTGTCGTGTCCTTGGATCATGTTTGAAAAATAACTGTGATGTTCAGTCACTTCATATATCTACTATATGGATGAGCTGTGTATGCGACAGTAACATAGTTTGTTATTTGAAACTAAAATTGGAATATAGATATTTTGTATATCTTCTGGCCAAATCTGCTATGGATGAAG

Intron 3 GTAATGCATT ... GTTGTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.78	539	4	356
A2	41.44	668.5	2	473
A3	41.27	697.5	14	490
A4	41.91	472.8	7	341
A5	42.74	351	2	262
A6	40.52	5533	3	3770
A7	41.12	5426	11	3789
A8	41.67	4184.5	3.5	2989
A9	40.07	5437.3	4.5	3635
A10	41.63	4763	2	3397

>TRINITY_DN110180_c0_g1_i5 from sample A3 / Example spliced isoform

CTTCGGTTTTTATTTTGCGTGGATTTGCAGTTTCACTAGCTCGGGGAATTCATTCACAGGTTCCAAGGCGGGAGAATTACTGTGTAATTTGGAGGATTTTAAGGGCAGATATGGGGAAGAGGAAGTCATCGAAGAAGCCGGAGAGCAAAAGAAAGCAGGAAAAACTCAGCACAGTCTTCAGCTGCCCTTTCTGCAACCAC
GACAGCAGCGTGGAGTGCCGCATGGATCGGAAAGATCTTATTGGGGAGGCCTCATGCAGGATATGCCAGGAGAGTTTCAGCACCTCAATAAATGCATTGAGCGAGCCCATTGACGTGTATTCGGAATGGATTGATGAATGTGAACGTGTAAATAATTCTGGATAAAATGGTTGCATTTGCATATGTCTTGAATATAGTAA
TTTGAGATATGGATGCTTTTTCAGTTGAGATTCTAACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCGCTTTACCCATAATGTATATTATAGGAAGCCATTTGAACAAATTCACAATATTCTACTCTGGCCTGTCATGTCCTTGGATCATGTTTGACAAATAACTGTGATGTTCAGTCACTTCATATATCTACTA
TATGGATGAGCAGTGTATATGACAGTAACATAGTTTGTTATTTGAAACTAAACTTGAAATATAGATATGTTGTATATCCTCTGGCCAAATCTGCTATGGATGAAGAATTTTTATATTTTCATTTTTTGCCTCTTGTTCTGTA

>TRINITY_DN110180_c0_g1_i8 from sample A3 / Example unspliced isoform:

CTTCGGTTTTTATTTTGCGTGGATTTGCAGTTTCACTAGCTCGGGGAATTCATTCACAGgtaatgcattgttgttttcgttaagccatttcgtgtgtgtttttgttatctctgccattaatttcctggttgtggcagGTTCCAAGGCGGGAGAATTACTGTGTAATTTGGAGGATTTTAAGGGCAGATATGGGGAAGAGG
AAGTCATCGAAGAAGCCGGAGAGCAAAAGAAAGCAGGAAAAACTCAGCACAGTCTTCAGCTGCCCTTTCTGCAACCACGACAGCAGCGTGGAGTGCCGCATGGATCGGAAAGATCTTATTGGGGAGGCCTCATGCAGGATATGCCAGGAGAGTTTCAGCACCTCAATAAATGCATTGAGCGAGCCCATTGACGTGTATTC
GGAATGGATTGATGAATGTGAACGTGTAAATAATTCTGGATAAAATGGTTGCATTTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTAACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCGCTTTACCCATAATGTATATTATAGGAAGCCATTTGAACAAATTCACAAT
ATTCTACTCTGGCCTGTCATGTCCTTGGATCATGTTTGACAAATAACTGTGATGTTCAGTCACTTCATATATCTACTATATGGATGAGCAGTGTATATGACAGTAACATAGTTTGTTATTTGAAACTAAACTTGAAATATAGATATGTTGTATATCCTCTGGCCAAATCTGCTATGGATGAAGAATTTTTATATTTTCAT
TTTTTGCCTCTTGTTCTGTA

Intron 4 GTAAGCTTCC ... ATGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	11.55	285.3	0	36
A2	7.92	265.3	0	22
A3	6.58	265.3	0	18
A4	9.44	167	2	17
A5	6.34	138.3	0	9
A6	10.34	2637.7	1	284
A7	8.2	2329.3	0	198
A8	9.28	2214.3	3	213
A9	8.07	2301.7	1	191
A10	8.57	1955.7	2	177

>TRINITY_DN43492_c0_g1_i1 from sample A8 / Example spliced isoform

GTGACTATAGATTGCACTCACGCATTTGGCTTCGGTTTTTATTTTGCGTGGATTTGCAGTTTCACTAGCTCGGGGAATTCATTCACAGGTTCCAAGGCGGGAGAATTACTGTGTAATTTGGAGGATTTTAAGGGCAGATATGGGGAAGAGGAAGTCATCGAAGAAGCCGGAGAGCAAAAGAAAGCAGGAAAAACTCAGCA
CAGTCTTCAGCTGCCCTTTCTGCAACCACGATAGCAGCGTGGAGTGCCGCATGGATCGGAAAGATCTTATTGGGGAGGCCTCATGCAGGATATGCCAGGAGAGTTTCAGCACCTCAATAAATGCATTGAGCGAGCCCATTGACGTGTATTCCGAATGGATTGATGAATGCGAACGTGTAAATAATTCTGGATAAAATGGT
TGCATTTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTGACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGCGCTTTACCCATAATGTATATTATAGGAAGCCATTTGAACAAATTCACAATATTCTACTCTGGCCTGTCATGTCCTTGGATCATGTTTGACAAATAACTG
TGATGTTCAGTCACTTCATATATCTACTATATGGATGAGCATTGTATATGACAGTAACATAGTTTGTTATTTGAAACTAAACTTGAAATATAGATATGTTGTATATCCTCTGGCCAAATCTGCTATGGATGAAGAATTTTTATATTTTCATTTTTTGCCTCTTGTTCTGTACGCCAATTCTGCTGTGGATGAAGAATTTT
TATATTTTCATTTTTTGCCTCTTGTTCTGTACGCCAATTCTGCTGTGGATGAAGAATTTTTATATTTT

>TRINITY_DN43492_c0_g1_i6 from sample A8 / Example unspliced isoform:

TTGAGCGAGCCCATTGACGTgtaagcttcctgaaccatatattttagtgtttgggttttgaccgattcctttttagaagtataattagacagctgaataggatgatatttatagagttttgagttattgaacaaagtttctccttatttggagcagaggaattttatacatggaataataaatagctgtatggatgatgc
ggccatttaaatgtctgaatatgtttgcagGTATTCCGAATGGATTGATGAATGCGAACGTGTAAATAATTCTGGATAAAATGGTTGCATTTGCATATGTCTTGAATATAGTAATTTGAGATATGGATGCTTTTTCAGTTGAGATTCTGACTGAAATCTGTGAGAGCATCTGCAGTTTGGATTTGC