sp|Q67ZB6|JMJ20_ARATH ----> Arginine-specific demethylase JMJ20

Intron 1 GTACATGAAG ... TGATCTTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	61.02	21	57.8	8
A2	49.67	18.8	26	11
A3	50.29	32	42.8	22
A4	45.73	27	33.5	12
A5	66.67	4	15	1
A6	41.77	46	41	25
A7	53.94	64.3	112.5	38
A8	48.85	55.8	72.8	33.8
A9	55.88	57.8	109.8	36.5
A10	50.39	72	105.3	41

>TRINITY_DN44943_c3_g1_i6 from sample A8 / Example spliced isoform

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAGAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAATCT
CAATGACAGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTG
AATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGAAGACACCATTTCAATAAATCACAACTGGT
TCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAG
CTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGT
TGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTA
GTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACC
GAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAA
GTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAAT
AAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCTGCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAA
TCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACATATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGG
ATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGCTCATCCTTCGTGGCCTTTATTTTTCAGGCAGTATGTTTTATATGGATTGTACTGGATTGA
TGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

>TRINITY_DN44943_c3_g1_i7 from sample A8 / Example unspliced isoform:

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCA
TGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGgt
acatgaagcagactgtctatgatatttatggagaggtctcagcaacacaattccctggattttctgagacatattggctggagtgtatccaagaaaggaatgaaattatctttgtacctagtggttggtttcatcaagtaaaaaatgtggtgtagggggcagaggtcagaggaatggagcaaggggcagccgagggctag
taaccagcaagattgaaggactgatcttgagGAAGACACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAG
GGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCAT
TACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGC
CTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTG
ATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGC
ATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTC
TTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTAC
CTGCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACA
CATATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTG
GCTCATCCTTCGTGGCCTTTATTTTTCAGGCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

Intron 2 GTACATGAAG ... GCTAATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.26	29	58	79
A2	51.61	30	26	38
A3	60	25	43	32
A4	56	33	34	50
A5	54.55	10	15	9
A6	67.06	28	41	73
A7	67.67	59	113	134
A8	60.57	69	73	139
A9	65.66	74	110	173
A10	64.33	61	105	115

>TRINITY_DN41639_c0_g1_i1 from sample A6 / Example spliced isoform

GGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCAATCCTCC
TCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATT
CAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGGATGAACT
TTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGAT
CAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATCAACACATCTGCCTCTGTACT
TGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTA
TTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCAT
TATAATGAAATGGCAGTCTAAGAATGTTATTTAC

>TRINITY_DN41639_c0_g1_i2 from sample A6 / Example unspliced isoform:

GAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAG
GTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGgtacatgaagcagactgtctatgatatttatggagaggtctcagcaacacaattccctggattttctgagacatattggctggagtgtatccaagaaaggaatgaaattatctttg
tacctagtggttggtttcatcaagtaaaaaatgtggaagagaggggtggacttgtggtccttgggcatcacgttgcaggaagagattcaacaaaatctttcatttcatctttgcgggagtaatcctcaccattccggcattcatcttcaagatccaggaagataccatttcaataaatcacaactggttcaatgcttgca
atatttcgtgggtgtggaacctccttgtaaatgactacaatgaaacaaaagaatatattgaagacataagaatcatatccgatgactttgagagcctctgtcaacgcaatttggcagctaatacagGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATC
ATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAA
TTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATA
ACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAAT
GTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTAC

Intron 3 GTAAGGATAG ... CTTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	32.8	35.5	3	17
A2	24.1	28.6	4.3	9
A3	33.61	25.8	1.3	13
A4	32.63	35.1	4.5	17
A5	0	4.3	3.3	0
A6	28.97	51.6	1.3	21
A7	22.76	72.6	6.6	21
A8	26.94	81	17	29
A9	27.95	97	12.6	37
A10	26.97	95.3	8.8	35

>TRINITY_DN127803_c0_g1_i4 from sample A1 / Example spliced isoform

GCTCGGAGAGGGCACATGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAAC
CAATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAAAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAAT
CTCAATGACAGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGG
TGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTT
ATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGAAGACACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGAC
TACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGA
CATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATG
ATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAG
ACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGTAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGG
CACGTCTTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGG
CAGTACCTGCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAAT
TCAACACATATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCT

>TRINITY_DN127803_c0_g1_i7 from sample A1 / Example unspliced isoform:

GCTCGGAGAGGGCACATGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAAC
CAATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAAAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAAT
CTCAATGACAGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGG
TGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTT
ATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGAAGATACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGgtaaggatagttatataatat
atatttagtatttgtagctggtcaaataatgctgtgaacaaacagcttgaacttgcatgcagtttcatttccattaaaaactgaaattcctttgtgcagTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGC
AGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGG
CATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATC
AACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGACAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGT
CCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAAT

Intron 4 GTTGCAGATT ... TGATCTTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	57.8	23	55	8
A2	60.82	19	48	11
A3	54.1	28	44	22
A4	51.9	19	29	12
A5	62.96	5	16	1
A6	45.86	36	36	25
A7	50.57	65	95	38
A8	45.36	53	55	33
A9	53.85	60	105	35
A10	52.11	68	107	41

>TRINITY_DN44943_c3_g1_i4 from sample A8 / Example spliced isoform

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCCCCAAATCCCGAGTTCAAGAAGATACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGA
AACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAG
CTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCAT
GAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCA
CGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATA
ATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGT
TCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATAT
CTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCTGCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAG
GTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACATATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACT
CAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGCTCATCCTTCGTGGCCTTTATTTTTCAGGCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACAT
GGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

>TRINITY_DN44943_c3_g1_i1 from sample A8 / Example unspliced isoform:

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAgttgcagattgtgacaataaagaattcacagaccagaagagactggggatgactgttgctgaatacatggaatactggggcaatct
caatgacagattttgtgagacggactttgatgggaacagaaataactcatcatcactttatttgaaggattggcattttgtaaaggaatatcctgactacaatgcatacactactccaacgttcttcttagatgattggcttaacttatacctggacacttatcgcatccatggcattcaaggtgttgatgaaggtggtg
aatgtgatgtgtcgtgctcagattatcgctttgtgtatatgggcccaaaagggacttggactccgttacatgcagatgtcttcaggtcatatagctggtccgcaaatgtttgtggcaaaaaactctggcattttctctcaccttcccaatcacatcttttgtatgacaggtacatgaagcagactgtctatgatatttat
ggagaggtctcagcaacacaattccctggattttctgagacatattggctggagtgtatccaagaaaggaatgaaattatctttgtacctagtggttggtttcatcaagtaaaaaatgtggtgtagggggcagaagtcagaggaacggagcaaggggcagccgagggctagtaaccagcaagattgaaggactgatcttg
agGAAGATACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTT
CTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAA
ATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAA
TATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAG
GCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTA
AGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATT
GCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCTGCAGTAAAATTATTGAGTGAAATGCAA
TGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACATATGGCTACATTCCCTGGCAATCTCAG
AAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGCTCATCCTTCGTGGCCTTTATTTTTCAG
GCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

Intron 5 GTGTAGGGGG ... GCTAATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.2	21	14	79
A2	80.43	10	5	38
A3	78.23	9	7	32
A4	83.43	10	7.3	50
A5	82.5	2	2	9
A6	88.27	10	5	73
A7	79.36	35.5	29	134
A8	81.15	33.5	20	139
A9	85.38	30.5	20.5	173
A10	79.53	30.5	21.3	115

>TRINITY_DN44943_c3_g1_i9 from sample A8 / Example spliced isoform

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCA
TGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGT
ACATGAAGCAGACTGTCTATGATATTTATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGGATGAACTTTGATGACCTTTTTGTCTTTC

>TRINITY_DN44943_c3_g1_i1 from sample A8 / Example unspliced isoform:

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAGAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAATCT
CAATGACAGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTG
AATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTTAT
GGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGgtgtagggggcagaagtcagaggaacggagcaaggggcagccgagggctagtaaccagcaagattgaaggactgatcttg
aggaagataccatttcaataaatcacaactggttcaatgcttgcaatatttcgtgggtgtggaacctccttgtaaatgactacaatgaaacaaaagaatatattgaagacataagaatcatatccgatgactttgagagcctctgtcaacgcaatttggcagctaatacagGGATGAACTTTGATGACTTTTTTGTCTTT
CTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAA
ATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAA
TATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAG
GCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTA
AGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATT
GCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCTGCAGTAAAATTATTGAGTGAAATGCAA
TGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACATATGGCTACATTCCCTGGCAATCTCAG
AAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGCTCATCCTTCGTGGCCTTTATTTTTCAG
GCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

Intron 6 GTACGTTTTT ... TTTTGTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	33	19	66
A2	68.35	25	20	54
A3	75	24	17	72
A4	59.32	24	11	35
A5	69.23	8	4	18
A6	55.77	23	10	29
A7	62.57	67	32	112
A8	61.74	44	23	71
A9	62.01	68	28	111
A10	61.49	62	26	99

>TRINITY_DN117034_c1_g1_i5 from sample A2 / Example spliced isoform

TGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCAATCCTCCTCACCGGCCTC
ATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGA
TGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGG

>TRINITY_DN117034_c1_g1_i6 from sample A2 / Example unspliced isoform:

TGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCAATCCTCCTCACCGGCCTC
ATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAgtacgtttttctctccattgattcttttgtccttttatttctcttcatcgtctcaggttcttcaatgccatgggaacccatttctgtcccgagtccataaagat
gttgcagattgtgacaataaagaattcacagaccagaaaagactggggatgactgttgctgaatacatggaatactggggcaatctcaatgacagattttgtgagacggactttgatgggaacagaaataactcatcatcactttatttgaaggattggcattttgtaaagGAATATCCTGACTACAATGCATACACTAC
TCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTG
GCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTTATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAA
AATGTGGAAGACACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGT
CTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGT
ATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAACCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAG
TCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCATGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGA
TAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAATTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAG
TCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGACTGGGTGTAAATGCTGGG

Intron 7 GTGTAGGGGG ... TGATCTTGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.75	42	14	8
A2	26.1	22.7	5	11
A3	31.18	32	7	22
A4	25.33	28	7	12
A5	20	6	2	1
A6	26.79	41	5	25
A7	26.27	94	29	38
A8	23.27	89	20	34
A9	25.74	83.7	21	37
A10	29.52	74	21	41

>TRINITY_DN44943_c3_g1_i2 from sample A8 / Example spliced isoform

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAGAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAATCT
CAATGACAGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTG
AATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTTAT
GGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGAAGACACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTA
CAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACA
TGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGAT
GCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGAC
AGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTC
ATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTA
ATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATA
TAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCTGCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGAT
GTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACATATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTC
TAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGCTCATCCTTCGTGGCCTTTATTTTTCAGGCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTG
TAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

>TRINITY_DN44943_c3_g1_i7 from sample A8 / Example unspliced isoform:

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCA
TGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGT
ACATGAAGCAGACTGTCTATGATATTTATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGgtgtagggggcagaggtcagaggaatggagcaaggggcagccgagggctag
taaccagcaagattgaaggactgatcttgagGAAGACACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAG
GGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCAT
TACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGC
CTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTG
ATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGC
ATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTC
TTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTAC
CTGCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACA
CATATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTG
GCTCATCCTTCGTGGCCTTTATTTTTCAGGCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

Intron 8 GTACATGAAG ... GCTAATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.37	28.7	57.3	79
A2	51.53	30	25.8	38
A3	60.47	24.4	42.6	32
A4	56.05	32.7	33.4	50
A5	54.55	10	15	9
A6	66.81	28.3	40.9	73
A7	67.59	59	112.1	134
A8	60.4	69.3	72.4	139
A9	65.61	74	109.4	173
A10	64.19	61.3	104.7	115

>TRINITY_DN41639_c0_g1_i1 from sample A6 / Example spliced isoform

GGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCAATCCTCC
TCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATT
CAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGGATGAACT
TTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGAT
CAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATCAACACATCTGCCTCTGTACT
TGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTA
TTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCAT
TATAATGAAATGGCAGTCTAAGAATGTTATTTAC

>TRINITY_DN41639_c0_g1_i6 from sample A6 / Example unspliced isoform:

GGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCAATCCTCC
TCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCCCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAGAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAATCTCAATGAC
AGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGA
TGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGgtacatgaagcagactgtctatgatatttatggagagg
tctcagcaacacaattccctggattttctgagacatattggctggagtgtatccaagaaaggaatgaaattatctttgtacctagtggttggtttcatcaagtaaaaaatgtggaagataccatttcaataaatcacaactggttcaatgcttgcaatatttcgtgggtgtggaacctccttgtaaatgactacaatgaa
acaaaagaatatattgaagacataagaatcatatccgatgactttgagagcctctgtcaacgcaatttggcagctaatacagGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGC
TTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATG
AGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCAC
GGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAA
TAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTAC

Intron 9 GTACATGAAG ... GCTAATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	70.26	29	58	79
A2	51.61	30	26	38
A3	60	25	43	32
A4	55.85	33	33.5	50
A5	54.55	10	15	9
A6	67.06	28	41	73
A7	67.67	59	113	134
A8	60.52	69	72.5	139
A9	65.66	74	110	173
A10	64.33	61	105	115

>TRINITY_DN44943_c3_g1_i11 from sample A8 / Example spliced isoform

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCA
TGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGG
ATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTA
CTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCT
CTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGAT
GATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCAT
TTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTT
CTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCT
GCAGTAAAATTATTGAGTGAAATGCAATGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACA
TATGGCTACATTCCCTGGCAATCTCAGAAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGC
TCATCCTTCGTGGCCTTTATTTTTCAGGCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

>TRINITY_DN44943_c3_g1_i1 from sample A8 / Example unspliced isoform:

TCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAACCA
ATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGTTGCAGATTGTGACAATAAAGAATTCACAGACCAGAAGAGACTGGGGATGACTGTTGCTGAATACATGGAATACTGGGGCAATCT
CAATGACAGATTTTGTGAGACGGACTTTGATGGGAACAGAAATAACTCATCATCACTTTATTTGAAGGATTGGCATTTTGTAAAGGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGGTG
AATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGgtacatgaagcagactgtctatgatatttat
ggagaggtctcagcaacacaattccctggattttctgagacatattggctggagtgtatccaagaaaggaatgaaattatctttgtacctagtggttggtttcatcaagtaaaaaatgtggtgtagggggcagaagtcagaggaacggagcaaggggcagccgagggctagtaaccagcaagattgaaggactgatcttg
aggaagataccatttcaataaatcacaactggttcaatgcttgcaatatttcgtgggtgtggaacctccttgtaaatgactacaatgaaacaaaagaatatattgaagacataagaatcatatccgatgactttgagagcctctgtcaacgcaatttggcagctaatacagGGATGAACTTTGATGACTTTTTTGTCTTT
CTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAA
ATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAA
TATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAG
GCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTA
AGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGCAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATT
GCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACATCGGAAATAGTACCCTCGTGATAATATAATATCTATTCTCTGCTCTCTGCCATCCTTCCTTTGGGAAATATGAGCACTAGCAACCAATGGATATGTGAGTAGAACAGGGCAGTACCTGCAGTAAAATTATTGAGTGAAATGCAA
TGGTCAGTCAACAATTCAATGGTCAGTCAACAATCAGCCATGATTCAGCAAGGGAGACACATGGGGAAAGGATTGTGTAGATGTGTAGGTCACTCATGTACAAACCTTTTCCCTCTTGAAGCAGGCAAATATTATATGGCTTACTGCTTTTATGATCATGCCAATTCAACACATATGGCTACATTCCCTGGCAATCTCAG
AAGGGAGCAGATACAAGAAAATGTTAATGATGGATGGATACCCCAGTTATCATGCAGCGTCAAATGAGTTGGGGTGGCCTTCTAAACTCAATGGTCATGGTCTGCCTCCTCTTGCTATCACAGTGCCTAGCACATAAAACTTATGAATCTTTATTGCTATTTCCAATTTTGGCTCATCCTTCGTGGCCTTTATTTTTCAG
GCAGTATGTTTTATATGGATTGTACTGGATTGATGGCACTGGCCATTTTTGCTTGCTTTAGTATATCTAGGAGGAATCTTTGTAACATGGTCTTTTTAGTTTAACCATGTTTCTAGTTTTGTGGTGATAGCCC

Intron 10 GTTGCAGATT ... GGTCCAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.46	27	55	52
A2	73.47	13	48	24
A3	63.89	26	44	48
A4	58.42	21	29	30
A5	67.35	8	16	17
A6	62.83	21	36	35
A7	63.17	58	95	104
A8	53.98	52	55	67
A9	67.05	57	105	127
A10	63.41	58	107	94

>TRINITY_DN46226_c2_g1_i10 from sample A7 / Example spliced isoform

ATGCATGCTCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCA
ACGAACCAATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGC
ATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTTATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGAAGATACC
ATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAAT
GGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAG
AAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAACCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAA
AATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTG
AGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATT
TACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGTAAGTTGTAGCAATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATTGCATATGGACT
TGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACA

>TRINITY_DN46226_c2_g1_i3 from sample A7 / Example unspliced isoform:

ATGCATGCTCGGAGAGGGCACCTGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCA
ACGAACCAATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAgttgcagattgtgacaataaagaattcacagaccagaagagactggggatgactgttgctgaatacatggaatactgg
ggcaatctcaatgacagattttgtgagacggactttgatgggaacagaaataactcatcatcactttatttgaaggattggcattttgtaaaggaatatcctgactacaatgcatacactactccaacgttcttcttagatgattggcttaacttatacctggacacttatcgcatccatggcattcaaggtgttgatga
aggtggtgaatgtgatgtgtcgtgctcagattatcgctttgtgtatatgggtccaaaagGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATG
ATATTTATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGAAGATACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTA
AATGACTACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAG
AAATGACATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCG
AAGATGATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAACCTAAAAATCACCAATGTAGACATTGTTGATGTTGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGA
ACAGAGACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGCCAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACT
CTCTGGTCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAATGTCTAGAGTTTCAAGCATTTTGCATTATAATGAAATGGCAGTCTAAGAATGTTATTTACAGGATTCTTTTGCTCACAAGTTTCAGTGCAACCTCTCGTCTGTCCATGGTAAGTTGTAGC
AATGAGTAATCAGTTCTTGATTGGGTGTAAATGCTGGGGATATACATGTCTTCTAAGGACATATTTACGCCAATGTCATGCTGAAATATGGCACGTCTTCTTTATGAATTCCACATTGACTGCATTGCATATGGACTTGTGAGTGTTAAAAAGAGTAATAAAATGGCCCATTCAATTTGACA

Intron 11 GTTGCAGATT ... TTTTGTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.71	33	55	66
A2	67.1	25	47.9	54
A3	69.88	24.9	43.6	71.9
A4	57.14	24	29	35
A5	68	8	16	18
A6	58.56	23	36	29
A7	60.7	67	95	112
A8	58.88	44	55	71
A9	61.36	68	105	111
A10	62.36	62	106.9	98.6

>TRINITY_DN127803_c0_g1_i1 from sample A1 / Example spliced isoform

GCTCGGAGAGGGCACATGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAAC
CAATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATC
CATGGCATTCAAGGTGTTGATGAAGGTGGTGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAG
GTACTTCTTGAAGATCACAAAAACAAGGTCTCTGTATACTTCAGTGGTTAGAGTGAAAGGACATAAGCAGGTGTATAATAATTCTTGCACTTTTCATCTTTGTTTTAAAAAAAGCTATTTTGATCTAATCAAGAAGTGAACCACATTTT

>TRINITY_DN127803_c0_g1_i10 from sample A1 / Example unspliced isoform:

GCTCGGAGAGGGCACATGTTTGAAACTCTTCCATACATACTCGGAGAGCAGATGTTTGAAGGCGAGGCAAATTGGAGGTGGCGAGACAAATTGGAGGTGTTAGAGGATGACGATCAAGGTAAGCGGGAAGATACGCCGAGTGGACGGAAGGCAGATCAGCTATGGAGAATTCAGAGATAGATATATGAAGGCCAACGAAC
CAATCCTCCTCACCGGCCTCATGGACGATTGGAGGGCTTGCCGCGATTGGGTATCTACTGATGGGCAACCCAATCTCTCCTTCTTCTCTTTAAACTTCGCCAAATCCCGAGTTCAAgttgcagattgtgacaataaagaattcacagaccagaaaagactggggatgactgttgctgaatacatggaatactggggcaat
ctcaatgacagattttgtgagacggactttgatgggaacagaaataactcatcatcactttatttgaaggattggcattttgtaaagGAATATCCTGACTACAATGCATACACTACTCCAACGTTCTTCTTAGATGATTGGCTTAACTTATACCTGGACACTTATCGCATCCATGGCATTCAAGGTGTTGATGAAGGTGG
TGAATGTGATGTGTCGTGCTCAGATTATCGCTTTGTGTATATGGGCCCAAAAGGGACTTGGACTCCGTTACATGCAGATGTCTTCAGGTCATATAGCTGGTCCGCAAATGTTTGTGGCAAAAAACTCTGGCATTTTCTCTCACCTTCCCAATCACATCTTTTGTATGACAGGTACATGAAGCAGACTGTCTATGATATTT
ATGGAGAGGTCTCAGCAACACAATTCCCTGGATTTTCTGAGACATATTGGCTGGAGTGTATCCAAGAAAGGAATGAAATTATCTTTGTACCTAGTGGTTGGTTTCATCAAGTAAAAAATGTGGAAGACACCATTTCAATAAATCACAACTGGTTCAATGCTTGCAATATTTCGTGGGTGTGGAACCTCCTTGTAAATGAC
TACAATGAAACAAAAGAATATATTGAAGACATAAGAATCATATCCGATGACTTTGAGAGCCTCTGTCAACGCAATTTGGCAGCTAATACAGGGATGAACTTTGATGACTTTTTTGTCTTTCTCACCCGAATGGCTCTTGCAAACTTTACCCAGCTCATGGATATCATGCACTCATGGGAAAGTGGATACAGCAGAAATGA
CATGGAAGCTTCAGAAATGAAGAAATTTCAACAGCTTCTCTTTAATTTGGTGTCCATTCGCGATGTTGCACAAAATATGGCATCCATTCATTACTTTGATCAGTATAGTAAAGAGTATAAATTTAATTTAGAAGATGTAGCTATGCTCTTTGTTGAAATGGGAAATTCGAAGGATTATAATAATCATGAACTCGAAGATG
ATGCCCATGAGAAAAAATGTCATGTATCACAGATTACAAATCTAAAAATCACCAATGTAGACATTGTTGATGTAGATATCAACACATCTGCCTCTGTACTTGAAGGATCAGAGAAGTCAATATATGATAAAAATTCTAGCAAGGTACAGATTAGTTTCTTCAATAACATCTCATCTGCCATGCCAGATCAAAGAACAGAG
ACAGTGCACGGCAACTTGTTAATTGATGGCTGCTTAGACAAAGATTGTCAGGTGACTGTTACTAATCCCAAACATCTCGTCCAAATTATTGATGATGTTATTGCATCAGTGAAAGATAAGGCTTTGAGTTGAGGCTATGATCTGGTCCAAACCGAAGAGAAAAATGTAGAGTGCTACTTCCTTGTTTTCTCACTCTCTGG
TCATGATAATAATAATAAAACTATAAGATAGCATTGTTTACTTCTGCCTGCTATAGATTATGTCTTCTGTCAAAT