sp|Q9SHZ8|PP168_ARATH ----> Pentatricopeptide repeat-containing protein At2g22070

Intron 1 GTAAGCATAG ... CCTGGATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	71.43	1	2	3
A2	57.14	3	4	4
A3	33.33	3	3	0
A4	-1	0	0	0
A5	-1	0	0	0
A6	72.73	3	5	11
A7	67.86	9	20	18
A8	61.64	14	23	22
A9	77.78	6	17	25
A10	69.57	7	18	14

>TRINITY_DN44455_c1_g1_i8 from sample A8 / Example spliced isoform

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTT
TTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATAT
ATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAAT
CTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGC
ACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAACATGGAATA
TCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCATTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTGCCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGATTAGACTT
GGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTATAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAATGTCATTGCTGG

>TRINITY_DN44455_c1_g1_i12 from sample A8 / Example unspliced isoform:

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAgtaagcataggcaacgatattaacaagcttatgcatattgctattatcatatgcctggatcagAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGAC
AATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAA
GCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGC
TCGATGTTTCTGGTGTGTGTGTCATGTTGCTTTTCTATTTATCTTATTTGAGAAATGAATATTTCAGTAAGAACATAAATAGAGCAAAGATCTTATACTGTGTTTGCAGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAAT
GGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCC
TGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAACATGGAATATCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCA
TTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTGCCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGATTAGACTTGGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTA
TAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAATGTCATTGCTGG

Intron 2 GTGAGGCTAA ... CTAAACTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.32	7	5	0
A2	42.86	2	2	1
A3	40	3	3	1
A4	100	0	0	1
A5	100	0	3	0
A6	77.78	2	3	11
A7	54.55	5	8	4
A8	51.85	13	11	17
A9	50	6	9	3
A10	60	8	18	6

>TRINITY_DN44455_c1_g1_i4 from sample A8 / Example spliced isoform

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTT
TATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTC
TTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATTCAAGATTGATGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTC
CGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTC
CATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAAC
ATGGAATATCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCATTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTGCCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGA
TTAGACTTGGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTATAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAATGTCATTGCTGG

>TRINITY_DN44455_c1_g1_i11 from sample A8 / Example unspliced isoform:

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGgtgaggctaaatgaaacaaccagtaagcataggcaacgatattaacaagcttatgcatattgctattatcatatgcctggatcaggtattatagtttgtttctgaaaaagtgtagggtttggactatggtaatggattgat
agtttctgatctaatctgggtgaatcactgattaactgatattgtattctaaactcagAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGTAAATCTTTATTTTCAAGGCAGGAACCTTTTAGATTTTAGGCAATGGGTTTTTGATTGTATATTATTGCCTTTGGATCTCTGTTAGAG
TATACTGACAATTAGTTGTTTGCATGCATTGTAGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTG

Intron 3 GTATTTTTGC ... TGTTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	81.82	2	11	7
A2	100	0	0	2
A3	54.08	3	6	1.1
A4	89.7	1	6	11.4
A5	-1	0	0	0
A6	86.67	1	7	6
A7	79.61	3	11	12.4
A8	80.21	4	18	14.4
A9	79.68	2	7	8.7
A10	75.63	2	9	3.4

>TRINITY_DN39580_c0_g1_i3 from sample A6 / Example spliced isoform

GGAGAAGGAACACATCCTTTGCCACCATAGTGAGAAGCTGGCAATCGCATTTGGACTGTTGAACACTTCCCATGGATCAACTATTCGAGTTATCAAGAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCA
AGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTATCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTA
CGAGGATACCTGTTAACCCAATGTTTCTGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTG
AGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCTAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATAAAGTGCAAGGCTTCGTTTGAAGGTTCCATGTGGCACCGCAGAGTACCATTGATGTAAGGCAATGTG
GGACTTCCTGCCCAAATATGTGAGTACCATTTTTCAATCAAATTTTGATTAGATTTAAATCTATATTATAATTCAAAGAAAAGAGACCCCTTAGACC

>TRINITY_DN39580_c0_g1_i2 from sample A6 / Example unspliced isoform:

GCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTA
AATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTATCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGgtatttttgca
tttcatgattgatgctttatccttccaagaatgcttactgctgaactgatgaagaaatggttaaaagtgcaaatagactgacgataacatatgccaaggtcatgaatcagagaaatgttgatagggcttaaagccttcacatccttgccacagaaaaatacattaaaagatttggcaattcccaaagctcaatatatgaa
gtggggcagtaggtttcttctcatatattttattgcccgttggatctgttatacagCATGCTCAAACTTGTTTGCCAAAAGGGATTATTCAAGATTGATGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACAT
ATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCTAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATAAAGTGCAAG
GCTTCGTTTGAAGGTTCCATGTGGCACCGCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTACCATTTTTCAATCAAATTTTGATTAGATTTAAATCTATATTATAATTCAAAGAAAAGAGACCCCTTAGACC

Intron 4 GTGAGGCTAA ... CCTGGATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.13	7	6	3
A2	63.64	2	3	4
A3	33.33	3	3	0
A4	-1	0	0	0
A5	-1	0	0	0
A6	80	2	5	11
A7	79.59	5	21	18
A8	61.19	13	19	22
A9	76	6	13	25
A10	70.91	8	25	14

>TRINITY_DN44455_c1_g1_i4 from sample A8 / Example spliced isoform

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTT
TATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTC
TTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATTCAAGATTGATGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTC
CGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTC
CATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAAC
ATGGAATATCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCATTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTGCCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGA
TTAGACTTGGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTATAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAATGTCATTGCTGG

>TRINITY_DN44455_c1_g1_i3 from sample A8 / Example unspliced isoform:

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGgtgaggctaaatgaaacaaccagtaagcataggcaacgatattaacaagcttatgcatattgctattatcatatgcctggatcagAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGAC
AATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAA
GCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATTCAAGATTGATGGCTCGATGGACTTTGTGAGGGTGATTTTTGGC
TCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCT
ACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCA
AACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAACATGGAATATCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCATTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTG
CCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGATTAGACTTGGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTATAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAA
TGTCATTGCTGG

Intron 5 GTAAGCATAG ... CTAAACTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.66	1	1	1
A2	14.29	3	0	1
A3	40	3	3	1
A4	100	0	1	1
A5	100	0	5	0
A6	67.35	3	3	9.5
A7	40.97	9	8	4.5
A8	51.01	13	9.5	17.5
A9	58.57	6	13	4
A10	58.79	7	13	7

>TRINITY_DN44455_c1_g1_i8 from sample A8 / Example spliced isoform

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTT
TTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATAT
ATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAAT
CTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGC
ACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAACATGGAATA
TCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCATTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTGCCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGATTAGACTT
GGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTATAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAATGTCATTGCTGG

>TRINITY_DN44455_c1_g1_i11 from sample A8 / Example unspliced isoform:

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAgtaagcataggcaacgatattaacaagcttatgcatattgctattatcatatgcctggatcaggtattatagtttgtttctgaaaaagtgtagggtttggactatggtaatggattgat
agtttctgatctaatctgggtgaatcactgattaactgatattgtattctaaactcagAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGTAAATCTTTATTTTCAAGGCAGGAACCTTTTAGATTTTAGGCAATGGGTTTTTGATTGTATATTATTGCCTTTGGATCTCTGTTAGAG
TATACTGACAATTAGTTGTTTGCATGCATTGTAGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTG

Intron 6 GCATGTAGAA ... ATTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	38.46	8	7	3
A2	66.67	2	2	6
A3	29.41	6	2	3
A4	80	2	9	7
A5	60	1	1	2
A6	60	2	4	2
A7	60	6	7	11
A8	73.91	3	7	10
A9	57.14	6	8	8
A10	64.29	5	5	13

>TRINITY_DN114872_c4_g1_i2 from sample A1 / Example spliced isoform

AGTGTTTGACAATATACCTAAACGAGATGTGGTCTCATGGACTACAATGATTGCAGGTTATGCCATGCATGGGCTTGTCAAGGAGGCCATCTGGCTCTTTGAACAAATGCAAGAATCTGGCACAACCCCGGATCATGTCACCTTCATCGGAGTTCTGTCTGCTTGCTGCCATGCAGGCTTGGTGGATGCTGCTAGGCATT
ACTTTGATTGTATGAGTCGAATTTATCATATCAAACCCGTAATGGAGCACTACTGCTGCATGGTTGACCTCCTTGGACGAGCTGGTCGCTTGGAAGAGGCACTAGACTTTATCAACAAAATGCCAACAACACCCAATGCGATCATTTGGGTTTCTCTTCTCAATGAGCTGGCAGGTGGGATGACATTGATAAGGTGCGAA
CAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAAAGTTTGTGCTGAACGATTTAGAC
AATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCTGGGATTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCA
CTTCAAGGAGGGTTTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTATCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGTAAATCTTTATTTTCAAGGACAATG
TTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCT
TCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGA
TGTTTCTGGTGTGTGTGTCATGTTGCTTTTCTATTTATCTTATTTGAGAAATGAATATTTCAGTAAGAACATAAATAGAGCAAAGATCTTATACTGTGTTTGCAGGATTTCTACATTGCTGAT

>TRINITY_DN114872_c4_g1_i1 from sample A1 / Example unspliced isoform:

AGTGTTTGACAATATACCTAAACGAGATGTGGTCTCATGGACTACAATGATTGCAGGTTATGCCATGCATGGGCTTGTCAAGGAGGCCATCTGGCTCTTTGAACAAATGCAAGAATCTGGCACAACCCCGGATCATGTCACCTTCATCGGAGTTCTGTCTGCTTGCTGCCATGCAGGCTTGGTGGATGCTGCTAGGCATT
ACTTTGATTGTATGAGTCGAATTTATCATATCAAACCCGTAATGGAGCACTACTGCTGCATGGTTGACCTCCTTGGACGAGCTGGTCGCTTGGAAGAGGCACTAGACTTTATCAACAAAATGCCAACAACACCCAATGCGATCATTTGGGTTTCTCTTCTCAATgcatgtagaatccattgcaacgtagagttaggagaa
caggtggcaaattacatttttgaattggagcctagaaatcctgtgccttatttgctgttattaaatatttatgcagGAGCTGGCAGGTGGGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGA
CCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAAAGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCTGGGATTCCTA
TCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTTTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTT
ATGCATATTGCTATTATCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGTAAATCTTTATTTTCAAGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTTTATCCTT
CCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTCTTCTCAT
ATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGTGTGTGTGTCATGTTGCTTTTCTATTTATCTTATTTGAGAAATGAATATTTCAGTAAGAACATAAATAGAGCAAAGATC
TTATACTGTGTTTGCAGGATTTCTACATTGCTGAT

Intron 7 GTAAATCTTT ... TGCATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	0	3.1	0	0
A2	0	2.1	0	0
A3	28.57	2.7	1	1
A4	35	2.7	1	0
A5	100	0	1	0
A6	54.59	7.3	8	0
A7	19.7	13.3	3	0
A8	45.97	14.9	12	0
A9	8.01	14.4	1	0
A10	49.29	6.9	6	4

>TRINITY_DN44455_c1_g1_i8 from sample A8 / Example spliced isoform

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTT
TTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAGTGCAAATAGACTGAGGATAACATATGCCAAGGTCATGAATTAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATAT
ATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAAT
CTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGC
ACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCTTAGACCAAAACATACATATCTACATTTGGTATTTATTATTCACTGTTGACCACCCGAAAATCCTGCAACATGGAATA
TCCATCACCGTAACCATCTAACAACCGCCTCTCTAAAGCATTGGGGTGGTTAGTTGATTCCTATTAGTGAAGATTTGATATTTTGAAGGATCTGGATTTAATTCTTGCCAAAGCTGGCCAGTGAGGATTGCGAGAATATAACAAGGGAAAGGCAAAAAGGGAAAACTAGACATAAAATTACGTATAAGTTGATTAGACTT
GGTATATGCCTTTGAATGGTCAAGAATATGAGGATTATTATAATTAGTTTATAGCATGTAAATCTACAAATGTAAGCCAAATATTCCATGTATATTATGAATAATAATGTCATTGCTGG

>TRINITY_DN44455_c1_g1_i11 from sample A8 / Example unspliced isoform:

GGATGACATTGATAAGGTGCGAACAATGATAAAAGACAGGGGGATAAAAAGGAAAGGAGGATGCAGCTGGATTGAAGTCAATAAGCAAATGCATACATTTGTTGTAGGCGACCGGACACACTCACAAACTCAAGAAATTTATGCAAAGTTGGAAACATTGTCAGGGCAGATGAGGGAGGCAGGGTATGTACCAGAAACAA
AGTTTGTGCTGAACGATTTAGACAATGAACAAAAGGATCAAATCCTTTGCCACCATAGTGAAAAACTCGCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTA
AGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTATCATATGCCTGGATCAGGTATTATAGTTTGTTTCTGAAAAAGTGTAGGGTTTGGACTATGGTAATGGATTGAT
AGTTTCTGATCTAATCTGGGTGAATCACTGATTAACTGATATTGTATTCTAAACTCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGgtaaatctttattttcaaggcaggaaccttttagattttaggcaatgggtttttgattgtatattattgcctttggatctctgttagag
tatactgacaattagttgtttgcatgcattgtagGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTG

Intron 8 GTATTATAGT ... CTAAACTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	7.69	2.5	0	0.5
A2	11.11	4	0	1
A3	100	0	0	1
A4	100	0	1	1
A5	100	0	5	0
A6	34.83	10.5	1	10.3
A7	19.24	17.3	4	4.2
A8	34.78	19.9	3.8	17.2
A9	25.91	23.6	13	3.5
A10	38.8	13.8	11	6.5

>TRINITY_DN39580_c0_g1_i3 from sample A6 / Example spliced isoform

GGAGAAGGAACACATCCTTTGCCACCATAGTGAGAAGCTGGCAATCGCATTTGGACTGTTGAACACTTCCCATGGATCAACTATTCGAGTTATCAAGAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCA
AGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTATCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTA
CGAGGATACCTGTTAACCCAATGTTTCTGCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTG
AGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCTAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATAAAGTGCAAGGCTTCGTTTGAAGGTTCCATGTGGCACCGCAGAGTACCATTGATGTAAGGCAATGTG
GGACTTCCTGCCCAAATATGTGAGTACCATTTTTCAATCAAATTTTGATTAGATTTAAATCTATATTATAATTCAAAGAAAAGAGACCCCTTAGACC

>TRINITY_DN39580_c0_g1_i7 from sample A6 / Example unspliced isoform:

GCACTTGCATTTGGGCTTATCAATACGTCCCCGGGGACTCCTATCCGGATCCTCAAAAACCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTA
AATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTATCATATGCCTGGATCAGgtattatagtttgtttctgaaaaagtgtagggtttggactatggtaatggattgatagtttctgatctaatctgggtgaatcactgattaactgatattgtattctaaactcagAAAAAATGAT
GATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGTAAATCTTTATTTTCAAGGCAGGAACCTTTTAGATTTTAGGCAATGGGG

Intron 9 GTGTGTGTGT ... GTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	1	2	2
A2	45.83	2.5	2	0
A3	46.67	2.5	2	0
A4	17.92	4.6	1	0
A5	0	6	0	1.5
A6	25	3	1	2
A7	27.41	13.3	5	4.5
A8	37.03	12	7	5.5
A9	15.18	16.9	3	5
A10	39.58	1.6	1	0

>TRINITY_DN44827_c7_g1_i7 from sample A7 / Example spliced isoform

TATTATTGCCTTTGGATCTCTGTTAGAGTATACTGACAATTAGTTGTTTGCATGCATTGTAGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATGGTTAAAAG
TGCAAATAGACTGACGATAACATATGCCAAGGTCATGAATCAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCATGCTCAAAC
TTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCT
TTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCA
AATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCT

>TRINITY_DN44827_c7_g1_i3 from sample A7 / Example unspliced isoform:

CCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTA
TCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATGTACGAGGATACCTGTTAACCCAATGTTTCTGGTATTTTTGCATTTCATGATTGATGCTTTATCCTTCCAAGAATGCTTACTGCTGAACTGATGAAGAAATG
GTTAAAAGTGCAAATAGACTGACGATAACATATGCCAAGGTCATGAATCAGAGAAATGTTGATAGGGCTTAAAGCCTTCACATCCTTGCCACAGAAAAATACATTAAAAGATTTGGCAATTCCCAAAGCTCAATATATGAAGTGGGGCAGTAGGTTTCTTCTCATATATTTTATTGCCCGTTGGATCTGTTATACAGCAT
GCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGgtgtgtgtgtcatgttgcttttctatttatcttatttgagaaatgaatatttcagtaagaacataaatagagcaaagatcttatactgtgtttgcagGATTTCTACATTG

Intron 10 GTACGAGGAT ... TGTTATACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.55	1.4	1.6	6.7
A2	42.86	1	0	1.7
A3	100	0	3.6	0.9
A4	92.55	0.7	6	10.4
A5	-1	0	0	0
A6	49.12	7	8.6	5.6
A7	67.74	4.7	8.4	11.4
A8	70.22	5.7	15	12.7
A9	44.2	9	8.1	7.4
A10	76.3	1.7	8.1	3

>TRINITY_DN44827_c7_g1_i5 from sample A7 / Example spliced isoform

CCTTAGAGTGTGTGGTGACTGCCATTCTGCCATCAAGTTTATCTCCAAAATTGTTGCAATGGAAATCATTGTAAGGGATGCCAGTCGGTTCCATCACTTCAAGGAGGGTCTTTGTTCCTGTGGGGATTATTGGTGAGGCTAAATGAAACAACCAGTAAGCATAGGCAACGATATTAACAAGCTTATGCATATTGCTATTA
TCATATGCCTGGATCAGAAAAAATGATGATCAGGAGAGACATCCTTGATACACACACATCAAAAGATTGGGACAATGTTGGGTTATGGTGGCACAAATCATGCTCAAACTTGTTTGCCAAAAGGGATTATACAAGATTGATGGCTCGATGGACTGTGTGAGGGTGATTTTTAGCTCGATGTTTCTGGATTTCTACATTGC
TGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGGAACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCG
ATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTGCCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCT

>TRINITY_DN44827_c7_g1_i4 from sample A7 / Example unspliced isoform:

TATTATTGCCTTTGGATCTCTGTTAGAGTATACTGACAATTAGTTGTTTGCATGCATTGTAGGACAATGTTGGGTTATGGTGGCACAAATgtacgaggatacctgttaacccaatgtttctggtatttttgcatttcatgattgatgctttatccttccaagaatgcttactgctgaactgatgaagaaatggttaaaag
tgcaaatagactgacgataacatatgccaaggtcatgaatcagagaaatgttgatagggcttaaagccttcacatccttgccacagaaaaatacattaaaagatttggcaattcccaaagctcaatatatgaagtggggcagtaggtttcttctcatatattttattgcccgttggatctgttatacagCATGCTCAAAC
TTGTTTGCCAAAAGGGATTATTCAAGATTGATGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGGACTTTGTGAGGGTGATTTTTGGCTCGATGTTTCTGGATTTCTACATTGCTGATTAAGACATATGATGAGTATGGACTCCGTTGAATCTGTGCTTGATCCTTTGGTTGTATGATTGTTGAGAATAATGG
AACTCAAGAATGGCAAAGGGATATATTCCTTTCCAAATCTCGAAATTTGTACCGAGTTGAGTTCTACAAACAAGATCTTAGATCATGCAGCAGACAATCAAAGAGCATAGCATTTTGGTCGATGAAGTGCAACGCTTCGTTTGAAGGTTCCATGTGGCACCTCAGAGTACCATTGATGTAAGGCAATGTGGGACTTCCTG
CCCAAATATGTGAGTATCATTTTTCAATCAAATTTTGATTAAATTTAAATCTATATTATAATTCAAACAAAAGAGACCCCT