sp|Q5NTA4|CHI4_CRYJA ----> Endochitinase 4

Intron 1 GTAAATGGCA ... TTTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.32	14	5	5
A2	29.03	22	12	6
A3	38.46	4	2	3
A4	40	6	4	4
A5	29.03	11	4	5
A6	27.54	25	15	4
A7	30.58	42	28	9
A8	31.71	14	5	8
A9	25.66	42	17	12
A10	25.4	47	18	14

>TRINITY_DN116106_c1_g1_i2 from sample A2 / Example spliced isoform

GGCTGGAAGTGGAGCAGCGAGCTGCTGCTTATTCATTTTACCCTGCATTGTCGAGCTGCGAGCAGCAGACTTGGGCACACACAGAACTTAGAAGGAAACAGCGCCATCTACAGCGTCCTCGCCCTCTCAGACACCAGGGTCCAGGACACACAGAAGGAAACAGAACCGTTGGCCGACGGCGACTCGGCACTGGGCAGTCC
CAACAAATCCGCGTGGAGGTCGACACAGGTTTGGATGTTTGCTTGCAACTTTCAACATATTGACAAGGATTTTGGCATCCATCAAGTTGTAAAAAAGCCTGCATGTGAACGGGTGGAAAAATGGTCAATGTCCCGTGAATAAAGATACAGAGATGTCGATAGCATTCTCGACAGACTACGTGTGAACCGGTGGAAAAATG
GTCAAAGTCCCGTAAGACACGTAACAGGATTCTTACGTCCTGTCAACGATAAAGTTATGTCCGAATTCAGTGTAAGAAGAATTGGAAATGAGATTTAATCAAATAACAGAATCCGGATTCCCCACGGCTATGGCTGCGGCTATATAAGCAAACTCCCTATTTCACCTATTTCATTGCAGTACGGATAGCCGGGCGCGCTT
CTCTTTATCTCTCTGTGGCAGCGAGATGAGTGATATGGGAACTGATCACCGTGTTAGGAAGACGAGCGTGTTTATTCTATGGCTTGCTCTTTCCCTTTATCTTCTTTCTATCTGCCGAGGAGATGTCCGGGACTTTGTCAC

>TRINITY_DN116106_c1_g1_i3 from sample A2 / Example unspliced isoform:

GGCTGGAAGTGGAGCAGCGAGCTGCTGCTTATTCATTTTACCCTGCATTGTCGAGCTGCGAGCAGCAGACTTGGGCACACACAGAACTTAGAAGGAAACAGCGCCATCTACAGCGTCCTCGCCCTCTCAGACACCAGGGTCCAGGACACACAGAAGGAAACAGAACCGTTGGCCGACGGCGACTCGGCACTGGGCAGTCC
CAACAAATCCGCGTGGAGGTCGACACAGgtaaatggcattgacatcccagtcccaatgtaaataaggtggcgaccatatgctaaattattactatgttcatagagttcacaatagatgggggcattaaagcaattgagttgttatggtgatctagcacttattctagagtctttctccctttttttcatattttgtttca
agatcattttttggttctatgtacatatgtaattttttttttttttcagGTTTGGATGTTTGCTTGCAACTTTCAACATATTGACAAGGATTTTGGCATCCATCAAGTTGTAAAAAAGCCTGCATGTGAACGGGTGGAAAAATGGTCAATGTCCCGTGAATAAAGATACAGAGATGTCGATAGCATTCTCGACAGACTAC
GTGTGAACCGGTGGAAAAATGGTCAAAGTCCCGTAAGACACGTAACAGGATTCTTACGTCCTGTCAACGATAAAGTTATGTCCGAATTCAGTGTAAGAAGAATTGGAAATGAGATTTAATCAAATAACAGAATCCGGATTCCCCACGGCTATGGCTGCGGCTATATAAGCAAACTCCCTATTTCACCTATTTCATTGCAG
TACGGATAGCCGGGCGCGCTTCTCTTTATCTCTCTGTGGCAGCGAGATGAGTGATATGGGAACTGATCACCGTGTTAGGAAGACGAGCGTGTTTATTCTATGGCTTGCTCTTTCCCTTTATCTTCTTTCTATCTGCCGAGGAGATGTCCGGGACTTTGTCAC

Intron 2 GTAAATGGCA ... TGTAAATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30	14	6	6
A2	31.25	22	14	6
A3	50	4	4	4
A4	57.14	6	8	8
A5	31.25	11	4	6
A6	31.51	25	16	7
A7	45.1	42	42	27
A8	46.15	14	11	13
A9	31.15	42	21	17
A10	42.68	47	35	35

>TRINITY_DN116106_c1_g1_i2 from sample A2 / Example spliced isoform

GGCTGGAAGTGGAGCAGCGAGCTGCTGCTTATTCATTTTACCCTGCATTGTCGAGCTGCGAGCAGCAGACTTGGGCACACACAGAACTTAGAAGGAAACAGCGCCATCTACAGCGTCCTCGCCCTCTCAGACACCAGGGTCCAGGACACACAGAAGGAAACAGAACCGTTGGCCGACGGCGACTCGGCACTGGGCAGTCC
CAACAAATCCGCGTGGAGGTCGACACAGGTTTGGATGTTTGCTTGCAACTTTCAACATATTGACAAGGATTTTGGCATCCATCAAGTTGTAAAAAAGCCTGCATGTGAACGGGTGGAAAAATGGTCAATGTCCCGTGAATAAAGATACAGAGATGTCGATAGCATTCTCGACAGACTACGTGTGAACCGGTGGAAAAATG
GTCAAAGTCCCGTAAGACACGTAACAGGATTCTTACGTCCTGTCAACGATAAAGTTATGTCCGAATTCAGTGTAAGAAGAATTGGAAATGAGATTTAATCAAATAACAGAATCCGGATTCCCCACGGCTATGGCTGCGGCTATATAAGCAAACTCCCTATTTCACCTATTTCATTGCAGTACGGATAGCCGGGCGCGCTT
CTCTTTATCTCTCTGTGGCAGCGAGATGAGTGATATGGGAACTGATCACCGTGTTAGGAAGACGAGCGTGTTTATTCTATGGCTTGCTCTTTCCCTTTATCTTCTTTCTATCTGCCGAGGAGATGTCCGGGACTTTGTCAC

>TRINITY_DN116106_c1_g1_i4 from sample A2 / Example unspliced isoform:

GGCTGGAAGTGGAGCAGCGAGCTGCTGCTTATTCATTTTACCCTGCATTGTCGAGCTGCGAGCAGCAGACTTGGGCACACACAGAACTTAGAAGGAAACAGCGCCATCTACAGCGTCCTCGCCCTCTCAGACACCAGGGTCCAGGACACACAGAAGGAAACAGAACCGTTGGCCGACGGCGACTCGGCACTGGGCAGTCC
CAACAAATCCGCGTGGAGGTCGACACAGgtaaatggcattgacatcccagtcccaatgtaaataagGTTTGGATGTTTGCTTGCAACTTTCAACATATTGACAAGGATTTTGGCATCCATCAAGTTGTAAAAAAGCCTGCATGTGAACGGGTGGAAAAATGGTCAATGTCCCGTGAATAAAGATACAGAGATGTCGATAG
CATTCTCGACAGACTACGTGTGAACCGGTGGAAAAATGGTCAAAGTCCCGTAAGACACGTAACAGGATTCTTACGTCCTGTCAACGATAAAGTTATGTCCGAATTCAGTGTAAGAAGAATTGGAAATGAGATTTAATCAAATAACAGAATCCGGATTCCCCACGGCTATGGCTGCGGCTATATAAGCAAACTCCCTATTT
CACCTATTTCATTGCAGTACGGATAGCCGGGCGCGCTTCTCTTTATCTCTCTGTGGCAGCGAGATGAGTGATATGGGAACTGATCACCGTGTTAGGAAGACGAGCGTGTTTATTCTATGGCTTGCTCTTTCCCTTTATCTTCTTTCTATCTGCCGAGGAGATGTCCGGGACTTTGTCAC

Intron 3 GTGGCGACCA ... TTTTTTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.45	6	1	5
A2	50	6	5	6
A3	42.86	4	2	3
A4	33.33	8	2	4
A5	45.45	6	0	5
A6	36.36	7	2	4
A7	25	27	2	9
A8	38.1	13	0	8
A9	41.38	17	1	12
A10	28.57	35	1	14

>TRINITY_DN116106_c1_g1_i4 from sample A2 / Example spliced isoform

GGCTGGAAGTGGAGCAGCGAGCTGCTGCTTATTCATTTTACCCTGCATTGTCGAGCTGCGAGCAGCAGACTTGGGCACACACAGAACTTAGAAGGAAACAGCGCCATCTACAGCGTCCTCGCCCTCTCAGACACCAGGGTCCAGGACACACAGAAGGAAACAGAACCGTTGGCCGACGGCGACTCGGCACTGGGCAGTCC
CAACAAATCCGCGTGGAGGTCGACACAGGTAAATGGCATTGACATCCCAGTCCCAATGTAAATAAGGTTTGGATGTTTGCTTGCAACTTTCAACATATTGACAAGGATTTTGGCATCCATCAAGTTGTAAAAAAGCCTGCATGTGAACGGGTGGAAAAATGGTCAATGTCCCGTGAATAAAGATACAGAGATGTCGATAG
CATTCTCGACAGACTACGTGTGAACCGGTGGAAAAATGGTCAAAGTCCCGTAAGACACGTAACAGGATTCTTACGTCCTGTCAACGATAAAGTTATGTCCGAATTCAGTGTAAGAAGAATTGGAAATGAGATTTAATCAAATAACAGAATCCGGATTCCCCACGGCTATGGCTGCGGCTATATAAGCAAACTCCCTATTT
CACCTATTTCATTGCAGTACGGATAGCCGGGCGCGCTTCTCTTTATCTCTCTGTGGCAGCGAGATGAGTGATATGGGAACTGATCACCGTGTTAGGAAGACGAGCGTGTTTATTCTATGGCTTGCTCTTTCCCTTTATCTTCTTTCTATCTGCCGAGGAGATGTCCGGGACTTTGTCAC

>TRINITY_DN116106_c1_g1_i3 from sample A2 / Example unspliced isoform:

GGCTGGAAGTGGAGCAGCGAGCTGCTGCTTATTCATTTTACCCTGCATTGTCGAGCTGCGAGCAGCAGACTTGGGCACACACAGAACTTAGAAGGAAACAGCGCCATCTACAGCGTCCTCGCCCTCTCAGACACCAGGGTCCAGGACACACAGAAGGAAACAGAACCGTTGGCCGACGGCGACTCGGCACTGGGCAGTCC
CAACAAATCCGCGTGGAGGTCGACACAGGTAAATGGCATTGACATCCCAGTCCCAATGTAAATAAGgtggcgaccatatgctaaattattactatgttcatagagttcacaatagatgggggcattaaagcaattgagttgttatggtgatctagcacttattctagagtctttctccctttttttcatattttgtttca
agatcattttttggttctatgtacatatgtaattttttttttttttcagGTTTGGATGTTTGCTTGCAACTTTCAACATATTGACAAGGATTTTGGCATCCATCAAGTTGTAAAAAAGCCTGCATGTGAACGGGTGGAAAAATGGTCAATGTCCCGTGAATAAAGATACAGAGATGTCGATAGCATTCTCGACAGACTAC
GTGTGAACCGGTGGAAAAATGGTCAAAGTCCCGTAAGACACGTAACAGGATTCTTACGTCCTGTCAACGATAAAGTTATGTCCGAATTCAGTGTAAGAAGAATTGGAAATGAGATTTAATCAAATAACAGAATCCGGATTCCCCACGGCTATGGCTGCGGCTATATAAGCAAACTCCCTATTTCACCTATTTCATTGCAG
TACGGATAGCCGGGCGCGCTTCTCTTTATCTCTCTGTGGCAGCGAGATGAGTGATATGGGAACTGATCACCGTGTTAGGAAGACGAGCGTGTTTATTCTATGGCTTGCTCTTTCCCTTTATCTTCTTTCTATCTGCCGAGGAGATGTCCGGGACTTTGTCAC