NA ----> NA

Intron 1 GTATGTTTCC ... AACATATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	4.5	0	4.5
A2	88.38	2.5	18	20
A3	53.8	8.8	12	9
A4	34.92	8	4	5
A5	32.72	11	5.8	5
A6	64.54	26	37	57.8
A7	62.69	23.3	37.3	41.8
A8	57.44	20.8	21.3	35
A9	47.91	32.8	22.5	37.5
A10	67.76	27	48.5	65.3

>TRINITY_DN117365_c0_g3_i6 from sample A3 / Example spliced isoform

TCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCACGATCAGTCTTTGAGTTGCCAGAAATCTGTTCCAAGGCTTTCGTCCATGGTGGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTT
GGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAA
CGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATT
GACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAG
GAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGAATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTA
CATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTG

>TRINITY_DN117365_c0_g3_i3 from sample A3 / Example unspliced isoform:

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGG
AAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttaaacatataagAAATCTGTTCCAAGGCTTTCGTCCATGGTGGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCC
TGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATG
AATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGC
TCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGT
TCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGA
ATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTG

Intron 2 GCTTTCGTCC ... CGTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	95.65	0.5	8	13.5
A2	63.34	4.5	5	10.5
A3	58.33	10	19	9
A4	61.66	8.5	19	8
A5	65.45	6	14	8.5
A6	68.34	20.5	44	40
A7	65.89	24	47	45.5
A8	69.26	17.5	34	45
A9	75.35	15.5	56	38.5
A10	62.19	20.5	47	20.5

>TRINITY_DN43420_c0_g3_i8 from sample A6 / Example spliced isoform

TAGACTACGGGTTCGACGATTTGTGTCGGAACCCGCTCACCGTCTACATTTCCCTGTTGAGGTCATATTTCCGATGATCGGCCAACTCCTAGCCGGAAAGGTTCTCACCGTCCGCTGGAAGGAGGCTCCAGCCTCCGCATGAGCCGGAAGGAAAAAGTAAAGGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATG
CATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGAAATGGAATCCGTAGTTCGCAAATGGC
TGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

>TRINITY_DN43420_c0_g3_i2 from sample A6 / Example unspliced isoform:

TAGACTACGGGTTCGACGATTTGTGTCGGAACCCGCTCACCGTCTACATTTCCCTGTTGAGGTCATATTTCCGATGATCGGCCAACTCCTAGCCGGAAAGGTTCTCACCGTCCGCTGGAAGGAGGCTCCGCATGAGCCGGAAGGAAAAAGTAAAGGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCC
TCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgctttcgtccatggttgtaagaaatccgattgccaggagagcg
aatttattgtggcaagactcctgtttagtggcgatggagatacccctgcaaggttttacagaagcggctccttgggagctagcggagccgagtattatatgagtttacagtcacgaagagggaagagttaaataagcaaggatcatattggaatgcatcatcttttgtgtcaacgagatcggcgagcactgtttctggaa
cgactcaagcggccgatgatgaatttgagagtgctgatgcagctgtttcagggatcaagaagaaaaatatcaacgaaaatgaatccagttattgggggtttcatcctgcccacatgtctcgccaaggaatatttcaatttatgatgaatattttgtattttttcatgcctgttgaaattcacgaagggtcacagtatccc
tctatcgacctgttcctctgctcagaatgtatgtgctctgacttgattacatatcattgtctgaggttccattgacagctgactgagcattacctataacaaggataggatggtcataacaggaacggaaatgtgaatttgagcatactgaaatacataccatggatgatggaatcctgctctgttcatttagattaacg
taaaatttcatgaaattttgttcaaatccgacacagattacgcagaattttacaatttatgatcgttttacagGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

Intron 3 GTATGTGCTC ... CGTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	77.78	8	28	12
A2	81.48	5	22	11
A3	73.08	7	19	6
A4	86.67	2	13	7
A5	81.25	3	13	8
A6	87.12	17	115	34
A7	80.98	31	132	42
A8	84.77	23	128	41
A9	78.05	27	96	39
A10	84.07	18	95	16

>TRINITY_DN44331_c1_g1_i11 from sample A9 / Example spliced isoform

TATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTAT
TGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGG
GAATCCGTAGTTCGCAAATGGC

>TRINITY_DN44331_c1_g1_i8 from sample A9 / Example unspliced isoform:

CAATTCTAAACTACTTGTTTTGTTTGGAAGTAGGCTACGAGTTCGACGATTTGTGTCGGAACCCGCTCACCGTCTACATTTCCCTGTTGAGGTCATATTTCCGATGATCGGCCAACTCCTAGCCGGAAAGGTTCTCACCGTCCGCTGGAAGGAGGCTCCGCATAGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGA
GGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGTGAGAACTCCTGTTCGACGCTAGGCTCTGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCACGATCAGTCTTTGAGTTGCCAGAAATCTGTTCCAAGGCTTTCGTCCATGGTGG
TAAGAAATCCGATTGCCAGGAGGCGCGAATTTATTGTGGCAACGGACTCCTGTTTAGTGGCGATGGAGATACCCCTACAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGACTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTC
AACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTG
TTGAAATTCACGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATgtatgtgctctgacttgattacatatcattgtctgaggtttcattgacagctgactgagcattacctataacaaggataggatggtcataacaggaacggaaatgtgaatttgagcatactgaaatacataccatggatgatg
gaatcctgctctgttcatttagattaatgtaaaatttcatgaaattttgttcaaatccgacacagattacgcagaattttacaatttatgatcgttttacagGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

Intron 4 GTATGTTTCC ... CGATTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	39.39	10	0	13
A2	56.35	9	10	13
A3	34.17	18.3	9	10
A4	28.93	17.3	0	14
A5	70	2	5	3
A6	52.65	47.7	29	77
A7	42.21	50.7	28	46
A8	38.86	50.3	12	52
A9	45.25	49.7	14	68
A10	56.4	41	39	67

>TRINITY_DN43420_c0_g3_i4 from sample A6 / Example spliced isoform

TAGACTACGGGTTCGACGATTTGTGTCGGAACCCGCTCACCGTCTACATTTCCCTGTTGAGGTCATATTTCCGATGATCGGCCAACTCCTAGCCGGAAAGGTTCTCACCGTCCGCTGGAAGGAGGCTCCAGCCTCCGCATGAGCCGGAAGGAAAAAGTAAAGGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATG
CATGGCCTCTCGCGCTCCACCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGAT
ACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCCAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCA
GCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGA
CTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAACGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTAC
GCAGAATTTTACAATTTATGATTGTTTTACAGGAAAACTTAAAATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTCGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGAATTTAAATACTGGAGAAGAG
TATTACTGCAAACAAATGAAGGGATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTGTGTATGAG
GAGCTCAGCATTATCTGTAATATAATCATGTGTTTCTTTCGCTGCACCATAGTCAAGAATATGCCCTTTATTACTTA

>TRINITY_DN43420_c0_g3_i1 from sample A6 / Example unspliced isoform:

TAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatataagaaatctgttccaaggctttcgtccatggttgtaagaaatccgattgcc
agGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGACTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTG
TTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCA
CAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTAC

Intron 5 GTATGTTTCC ... CGTTTTACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	0	14
A2	73.68	5	18	10
A3	47.37	10	9	9
A4	44	7	4	7
A5	56.52	5	5	8
A6	71.43	13	32	33
A7	63.33	22	33	43
A8	62.22	17	16	40
A9	65.43	14	19	34
A10	61.17	20	45	18

>TRINITY_DN43420_c0_g3_i8 from sample A6 / Example spliced isoform

TAGACTACGGGTTCGACGATTTGTGTCGGAACCCGCTCACCGTCTACATTTCCCTGTTGAGGTCATATTTCCGATGATCGGCCAACTCCTAGCCGGAAAGGTTCTCACCGTCCGCTGGAAGGAGGCTCCAGCCTCCGCATGAGCCGGAAGGAAAAAGTAAAGGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATG
CATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGAAATGGAATCCGTAGTTCGCAAATGGC
TGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

>TRINITY_DN43420_c0_g3_i6 from sample A6 / Example unspliced isoform:

GACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttttgttttatcgtatacaagttagttaaacatataaga
aatctgttccaaggctttcgtccatggttgtaagaaatccgattgccaggagagcgaatttattgtggcaagactcctgtttagtggcgatggagatacccctgcaaggttttacagaagcggctccttgggagccagcggagccgagtattatatgagtttacagtcacgaagagggaagagttaaataagcaaggatc
atattggaatgcatcatcttttgtgtcaacgagatcggcgagcactgtttctggaacgactcaagcggccgatgatgaatttgagagtgctgatgcagctgtttcagggatcaagaagaaaaatatcaacgaaaatgaatccagttattgggggtttcatcctgcccacatgtctcgccaaggaatatttcaatttatga
tgaatattttgtattttttcatgcctgttgaaattcacgaagggtcacagtatccctctatcgacctgttcctctgctcagaatgtatgtgctctgacttgattacatatcattgtctgaggttccattgacagctgactgagcattacctataacaaggataggatggtcataacaggaacggaaatgtgaatttgagc
atactgaaatacataccatggatgatggaatcctgctctgttcatttagattaacgtaaaatttcatgaaattttgttcaaatccgacacagattacgcagaattttacaatttatgatcgttttacagGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCG
TAGTTCGCAAATGGC

Intron 6 GCTTTCGTCC ... CGATTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	55.56	10	8	17
A2	44.05	7	4	7
A3	58.72	13.5	17	19
A4	58.63	12.5	16.5	18
A5	92.9	1	13.3	13
A6	56.24	42.5	43.3	65.8
A7	56.33	38.5	42.3	53.8
A8	50.19	43.5	30.8	56
A9	59.83	44	54	76.8
A10	61.57	33.5	44.3	62.3

>TRINITY_DN117365_c0_g3_i4 from sample A3 / Example spliced isoform

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGG
AAGGCCCGATCAGTCTTTGAGTTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTT
TGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCA
TGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGG
ATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTG
GGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGAATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAG
ACAAACTCCCTTCTTTTCTCTATTG

>TRINITY_DN117365_c0_g3_i1 from sample A3 / Example unspliced isoform:

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTT
CAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgctttcgtccatggtggtaagaaatccgattgccagGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAA
GAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGT
CTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCAT
AACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCA
CAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGAATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAA
ATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTG

Intron 7 GTATGTTTCC ... CTGTTCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.79	8	0	3
A2	69.7	5	10	13
A3	32.14	19	9	9
A4	15.56	19	0	7
A5	24.32	14	5	4
A6	49.41	43	29	55
A7	37.75	47	28	29
A8	36.45	34	12	27
A9	33.73	56	14	43
A10	51.58	46	39	59

>TRINITY_DN43420_c0_g3_i2 from sample A6 / Example spliced isoform

TAGACTACGGGTTCGACGATTTGTGTCGGAACCCGCTCACCGTCTACATTTCCCTGTTGAGGTCATATTTCCGATGATCGGCCAACTCCTAGCCGGAAAGGTTCTCACCGTCCGCTGGAAGGAGGCTCCGCATGAGCCGGAAGGAAAAAGTAAAGGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCC
TCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGCTTTCGTCCATGGTTGTAAGAAATCCGATTGCCAGGAGAGCG
AATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAA
CGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCACAGTATCCC
TCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTCCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAACG
TAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

>TRINITY_DN43420_c0_g3_i1 from sample A6 / Example unspliced isoform:

TAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatataagaaatctgttccaagGCTTTCGTCCATGGTTGTAAGAAATCCGATTGCC
AGGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGACTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTG
TTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCA
CAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTAC

Intron 8 GTATGTTTTC ... CGATTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	45.91	10	0	17
A2	74.73	6	22.5	13
A3	65.59	8	20	10.5
A4	61.25	9	12.5	16
A5	88.19	1	9	6
A6	60.08	39	42.5	75
A7	68.33	27	55	61.5
A8	56.21	37	34.5	60.5
A9	54.02	40	28	66
A10	69.48	28	56	71.5

>TRINITY_DN46679_c0_g2_i2 from sample A10 / Example spliced isoform

GCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCCAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGA
GTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAG
GAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAAC
GGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAACGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATTGTTTTACAGGAAAACTTAAAATTATATTCAATCTGAGTGGAAT

>TRINITY_DN46679_c0_g2_i7 from sample A10 / Example unspliced isoform:

GAAGGAGGCTCCGCATGAGCCGGAAGGAAAAAGTAAAGGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTTCGCTCCCCTCAAGTTTCTGCGACTAGTT
CAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgttttcattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttttattttatcgtatacaagttagttaaacatataagaaatctgttccaaggctttcgtccatggtggtaagaaatcc
gattgccagGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCCAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCG
AGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGA
AGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTAC

Intron 9 GTATGTTTCC ... CGTATATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	17.78	4.7	0	2
A2	89.9	1.7	12.7	17
A3	52.57	9	12.3	7.7
A4	28.89	8	3.3	3.3
A5	33.94	11	7	4.3
A6	64.56	25.7	37.7	56
A7	58.69	23.3	38	28.7
A8	54.29	18.3	20	23.3
A9	42.28	33	18.7	29.7
A10	67.2	25.3	44.3	59.3

>TRINITY_DN43420_c0_g3_i12 from sample A6 / Example spliced isoform

CCGCTGGAAGGAGGCTCCGCATGGGCCGGAAAGAAAAAGTACGGGAGACTCTAATTCGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTG
GAAGGCACGATCAGTCTTTGAGTTGCCAGAAATCTGTTCCAAGGCTTTCGTCCATGGTTGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGACTATTATATGAGTTTACAGTCAC
GAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACA
TGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTAC

>TRINITY_DN43420_c0_g3_i1 from sample A6 / Example unspliced isoform:

TAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatataagAAATCTGTTCCAAGGCTTTCGTCCATGGTTGTAAGAAATCCGATTGCC
AGGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGACTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTG
TTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCA
CAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTAC

Intron 10 GTATGTTTCC ... CTGTTCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.7	8	0	5.6
A2	80.27	4.7	19.6	18
A3	42.6	16.7	15.9	9
A4	29.84	17.3	6.6	8
A5	31.12	13.3	7	5
A6	55.31	41.4	40.1	62.4
A7	49.47	44.6	44	42.9
A8	49.67	32.1	28	36
A9	40.33	55.7	25.7	49.6
A10	56.4	45.9	53.6	64.7

>TRINITY_DN117365_c0_g3_i1 from sample A3 / Example spliced isoform

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGCGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTT
CAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGCTTTCGTCCATGGTGGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAA
GAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGT
CTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCAT
AACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCA
CAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGAATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAA
ATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTG

>TRINITY_DN117365_c0_g3_i3 from sample A3 / Example unspliced isoform:

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGG
AAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttaaacatataagaaatctgttccaagGCTTTCGTCCATGGTGGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCC
TGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATG
AATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGC
TCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGT
TCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGA
ATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTG

Intron 11 GTATGTTTTC ... AACATATAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.26	6	0	4.5
A2	90.29	2.3	19.5	20.5
A3	34.85	13	12.5	6.5
A4	46.37	10.5	9	8.8
A5	29.56	12	6.5	5
A6	60.27	30.5	33.8	46
A7	56.23	32	39.3	40
A8	53.76	26.3	22.3	30.3
A9	48.05	38.8	22	35.5
A10	61.51	33	44.5	52.3

>TRINITY_DN101832_c0_g1_i3 from sample A5 / Example spliced isoform

CGCTGGAAGGAGGCTCCGCATAGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGTGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTTCGCTCCCCTCAAGTTTCTGCGAC
TAGTTCAAACAACGTGGAAGGCACGATCAGTCTTTGAGTTGCCAGAAATCTGTTCCAAGGCTTTCGTCCATGGTGGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTAT
ATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGG
TTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATA
ACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAATGGAATCCGTAGTTCGCAAAT
GGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

>TRINITY_DN101832_c0_g1_i1 from sample A5 / Example unspliced isoform:

CGCTGGAAGGAGGCTCCGCATAGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGTGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTTCGCTCCCCTCAAGTTTCTGCGAC
TAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttttattttatcgtatacaagttagttaaacatataagAAATCTGTTCCAAGGCTTTCGTCCATGGTGGTAAGA
AATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGAT
CGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATT
CATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTG
CTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGG

Intron 12 GTATGTTTCC ... CGATTGCCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.71	10	0	16.2
A2	62.72	9.4	18.3	13
A3	38.32	17.7	12.3	10.1
A4	35.59	17.3	4.2	15
A5	75.05	2	6	5
A6	55.27	46.9	37.5	78.5
A7	48.98	50.3	37.7	59.3
A8	45.1	50.1	21.7	61
A9	48.34	49.5	22.7	70
A10	59.47	41.3	49.2	72.1

>TRINITY_DN117365_c0_g3_i4 from sample A3 / Example spliced isoform

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGG
AAGGCCCGATCAGTCTTTGAGTTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTT
TGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCA
TGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGG
ATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTG
GGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGAATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAG
ACAAACTCCCTTCTTTTCTCTATTG

>TRINITY_DN117365_c0_g3_i3 from sample A3 / Example unspliced isoform:

GCTCCAGCCTCCGCATGGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCATTCGACATTCAAACACAGCGAGAACTCCTGTTCGACGCATTGGCTCCGCTCCCCTGTTCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGG
AAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttaaacatataagaaatctgttccaaggctttcgtccatggtggtaagaaatccgattgccagGAGGGCGAATTTATTGTGGCAAGACTCC
TGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATG
AATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGC
TCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGT
TCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAAACTTAAGATTATATTCAATCTGAGTGGAATCCGTAGTTCACAAATGGCTGATCGGGAATTTTAGAGATTTTCCTGTTTCATTTATTTGGGGTTCCTGCAAGTTTGTGATTCTGGAGAAGAGTATTACTGCAAACAAATGAAGGA
ATTTAAATAGATTTCATATCCAAACAAGAATCCTTGCATAGTCAGTTGATTACATCAGATATTCTCACTTGTGGAGGTTATCTCAGTTCTTACAAAATCAGCTTTTTGTTGTTCCCCAGTCTCGGTATTTGACATGTCTGTAAGACAAACTCCCTTCTTTTCTCTATTG

Intron 13 GTATGTTTCC ... CTGTTCCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.43	6.7	0	6.1
A2	79.65	5.1	22.3	17.7
A3	41.66	18	18.8	7
A4	40.07	17.2	11.9	11
A5	34.73	13	8.8	5
A6	56.49	38.1	43	53
A7	52.51	43.8	53.9	42.4
A8	50.83	31.8	32.6	33
A9	43.53	49.1	28.1	45.1
A10	57.39	42.8	55.7	57.7

>TRINITY_DN101832_c0_g1_i5 from sample A5 / Example spliced isoform

CGCTGGAAGGAGGCTCCGCATAGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGTGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTTCGCTCCCCTCAAGTTTCTGCGAC
TAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGCTTTCGTCCATGGTGGTAAGAAATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTC
ACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCA
CATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATG
GTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAAT
CCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGC

>TRINITY_DN101832_c0_g1_i1 from sample A5 / Example unspliced isoform:

CGCTGGAAGGAGGCTCCGCATAGGCCGGAAGGAAAAAGTAAAAGAGACTCAGTTTGGAGGGCCCGCCGCATGCATTCAATGCATGGCCTCTCGCGCTCCACCCACTCCAGCCCATTCGACATTCAAACACAGTGAGATCGACGCATTGGCTCCGCTCCCCTGTTCGACGCTAGGCTTCGCTCCCCTCAAGTTTCTGCGAC
TAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGgtatgtttccattcagggttctgcttacctggtcgctagatgttgcgtacgaactaacttattttatcgtatacaagttagttttattttatcgtatacaagttagttaaacatataagaaatctgttccaagGCTTTCGTCCATGGTGGTAAGA
AATCCGATTGCCAGGAGGGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGAT
CGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTCATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATT
CATGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTTCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGCATACTGAAATACATACCATGGATGATGGAATCCTG
CTCTGTTCATTTAGATTAATGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGG

Intron 14 ATACAAGTTA ... TTAGTTAAAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	33.33	2	0	2
A2	54.29	16	18	20
A3	42.86	10	8	7
A4	77.78	2	6	8
A5	57.89	4	7	4
A6	40.83	50	33	36
A7	59.63	22	33	32
A8	50	23	19	27
A9	51.85	26	23	33
A10	44.04	61	46	50

>TRINITY_DN43420_c0_g3_i1 from sample A6 / Example spliced isoform

TAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGTATGTTTCCATTCAGGGTTCTGCTTACCTGGTCGCTAGATGTTGCGTACGAACTAACTTATTTTATCGTATATAAGAAATCTGTTCCAAGGCTTTCGTCCATGGTTGTAAGAAATCCGATTGCC
AGGAGGGCGAATTTATTGTGGCAACGCTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCTAGCGGAGCCGACTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATCATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTG
TTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGATGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCA
CAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTAC

>TRINITY_DN43420_c0_g3_i6 from sample A6 / Example unspliced isoform:

GACGCTAGGCTCCGCTCCCCTCAAGTTTCTGCGACTAGTTCAAACAACGTGGAAGGCCCGATCAGTCTTTGAGTTGCCAGGTATGTTTCCATTCAGGGTTCTGCTTACCTGGTCGCTAGATGTTGCGTACGAACTAACTTATTTTATCGTatacaagttagttttgttttatcgtatacaagttagttaaacATATAAGA
AATCTGTTCCAAGGCTTTCGTCCATGGTTGTAAGAAATCCGATTGCCAGGAGAGCGAATTTATTGTGGCAAGACTCCTGTTTAGTGGCGATGGAGATACCCCTGCAAGGTTTTACAGAAGCGGCTCCTTGGGAGCCAGCGGAGCCGAGTATTATATGAGTTTACAGTCACGAAGAGGGAAGAGTTAAATAAGCAAGGATC
ATATTGGAATGCATCATCTTTTGTGTCAACGAGATCGGCGAGCACTGTTTCTGGAACGACTCAAGCGGCCGATGATGAATTTGAGAGTGCTGATGCAGCTGTTTCAGGGATCAAGAAGAAAAATATCAACGAAAATGAATCCAGTTATTGGGGGTTTCATCCTGCCCACATGTCTCGCCAAGGAATATTTCAATTTATGA
TGAATATTTTGTATTTTTTCATGCCTGTTGAAATTCACGAAGGGTCACAGTATCCCTCTATCGACCTGTTCCTCTGCTCAGAATGTATGTGCTCTGACTTGATTACATATCATTGTCTGAGGTTCCATTGACAGCTGACTGAGCATTACCTATAACAAGGATAGGATGGTCATAACAGGAACGGAAATGTGAATTTGAGC
ATACTGAAATACATACCATGGATGATGGAATCCTGCTCTGTTCATTTAGATTAACGTAAAATTTCATGAAATTTTGTTCAAATCCGACACAGATTACGCAGAATTTTACAATTTATGATCGTTTTACAGGAAATGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCGTAGTTCGCAAATGGCTGATCGGGAATCCG
TAGTTCGCAAATGGC