sp|Q9SHC8|VAP12_ARATH ----> Vesicle-associated protein 1-2

Intron 1 GTAAGATAAT ... TCTTATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	31.03	60	27	2
A2	30.16	44	19	0
A3	26.74	63	23	1
A4	22	39	11	0
A5	22.03	46	13	0
A6	19.39	133	32	0
A7	15.07	186	33	0
A8	14.29	84	14	0
A9	13.75	138	22	0
A10	12.26	136	19	0

>TRINITY_DN109668_c0_g1_i6 from sample A1 / Example spliced isoform

TTGAGTTAACAAACTTAACCACGGTTTACGTTTGCCAGTACATTTCTACTGGAATTTCTGAGATTCTTTTCTTCTCTTCAACCCCCACGGCATTCCCCACAGCATTTATTACATTGCTCAGTTCATCGAAGCTCAACTCCACCCTCACAGCTCGAAGCCATCCTGTGGAAAAGAAGCATACTGTGCTAATCGGCCAAAGA
AATCCTCACTCTCCGATTACAGGCGCTGTGAAACTATTTGAATTCGCGTGCCACTGGAAAACCAGGACCGACATTGCTAGGGCTGTAAAATTGATCTGAATATGACTTGGCAAACGTCTTCAGATTTCGTTTTTTGAGCGCTACAGGAGCGGGGTTCTGGGAGCCTCGAGGTCTGAAAGATCCCAGTTCATTTCTGGTTG
CCTCAATTGCTGCTTGGAGGAGTTGATCTGTTCGTAAAATGGGGCTTTGCAAGAGCTGATCTGAAGAGGTTGCTTTGAGGGTTGCGTTTGGCCTGGGCATTTGTACCGGAGTTTAAGCTCAAGTTCGGGGTAAAGAATGAGTAACGGCGAGCTTCTGACGATTCATCCTACTGAGCTTAAATTCCCGTTCGAACTTAAGA
AGCAGAGTTCATGTTCTCTTCAGTTGGTAAATAATTCAGATCATTATGTGGCCTTCAAGGTGAAAACTACATCCCCAAAGAAGTACTGTGTACGACCCAACACTGGTGTTGTGCTGCCTAAGTCTACTTGCGATGTGATAGTCACTATGCAATCACAGCGAGAAGCCCCTCCTGATCTGCAGTGCAAAGACAAATTCCTT
GTGCAGAGCGTTGTAGCACCTTATGGGGTAGATCAGAAGGATATCACTCCGGAAATGTTCATCAAGGACGATGGGAAGGTTGTTGAGGAGTGCAAGCTCAGGGTGGTTTATGCTCCTCCGCCCCAACCTCCTTCTCCAGTTCCTGAAGGATCAGAAGAGGGCTCATCTCCAAGGGCGTTGTCATCCGACAATGGACAACA
GAATACTGGTTTGTTCAAAGTGGCACCCAAAGAACTTGGTGAATTGAAGGCAAAACTTACTGAGGCACGCACAATGCTGTCAAAATTAACAGATGAAAGGAATACAGTTGCGCAGCAAACTCAAGAACTACGTCAGGAATTGGTATGTTTCAAGATTAGTTACTTCCCTTCGAATTTATAGCCCTTGTATTATTGCACTT
TTTTGCGTCTTCCGTTTCATATTTTTTGATGTATTTTTTTTTAATTTCTTTGGACATATTCATTTATATTGTATGTGACTCTCTGAGAATGGCTGTGATTATGGGAGCTGAATAGTCTGAAGTACTTTGTGCA

>TRINITY_DN109668_c0_g1_i1 from sample A1 / Example unspliced isoform:

ATCGCAGCTTTGATTTTTGTCGTGATGTATAACATCATTAATAATCTTCCAACACTATTGCTGGTAGATTCACAGTGGATTAGAGAGTGAAGACCAAATTTATAACATACTCGAGGCGTTCATGTTTTTATGCTCGCTGTTTAAAGGTGGATTCCATTTCAGTTCATTTCTGGTTGCCTCAATTGCTGCTTGGAGGAGTT
GATCTGTTCGTAAAATGGGGCTTTGCAAGAGCTGATCTGAAGAGGTTGCTTTGAGGGTTGCGTTTGGCCTGGGCATTTGTACCGGAGTTTAAGCTCAAGTTCGGGGTAAAGAATGAGTAACGGCGAGCTTCTGACGATTCATCCTACTGAGCTTAAATTCCCGTTCGAACTTAAGAAGCAGAGTTCATGTTCTCTTCAGT
TGGTAAATAATTCAGATCATTATGTGGCCTTCAAGGTGAAAACTACATCCCCAAAGAAGTACTGTGTACGACCCAACACTGGTGTTGTGCTGCCTAAGTCTACTTGCGATGTGATAGTCACTATGCAATCACAGCGAGAAGCCCCTCCTGATCTGCAGTGCAAAGACAAATTCCTTGTGCAGAGCGTTGTAGCACCTTAT
GGGGTAGATCAGAAGGATATCACTCCGGAAATGgtaagataattttatgtcagaagctcttagtgccataattcatgtaatttacttttttcacatgtgaatcattactgtcttatgcagTTCATCAAGGACGATGGG

Intron 2 GTATTCTGGT ... TTTGCGTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.67	34	86	50
A2	69.64	17	33	45
A3	62.16	35	51	64
A4	75.19	16	57	40
A5	87.69	4	15	42
A6	69.54	106	218	266
A7	70.07	91	207	219
A8	68.55	128	246	312
A9	66.77	109	203	235
A10	70.87	67	138	188

>TRINITY_DN104464_c0_g1_i1 from sample A3 / Example spliced isoform

TCTTCTCTTCAACCCCCACGGCATTCCCCACAGCATTTATTACATTGCTCAGTTCATCGAAGCTCAACTCCACCCTCACAGCTCGAAGCCATCCTGTGGAAAAGAAGCATACTGTGCTAATCGGCCAAAGAAATCCTCACTCTCCGATTACAGGCGCTGTGAAACTATTTGAATTCGCGTGCCACTGGAAAACCAGGACC
GACATTGCTAGGGCTGTAAAATTGATCTGAATATGACTTGGCAAACGTCTTCAGATTTCGTTTTTTGAGCGCTACAGGAGCGGGGTTCTGGGAGCCTCGAGGTCTGAAAGATCCCAGGTGGATTCCATTTCAGTTCATTTCTGGTTGCCTCAATTGCTGCTTGGAGGAGTTGATCTGTTCGTAAAATGGGGCTTTGCAAG
AGCTGATCTGAAGAGGTTGCTTTGAGGGTTGCGTTTGGCCTGGGCATTTGTACCGGAGTTTAAGCTCAAGTTCGGGGTAAAGAATGAGTAACGGCGAGCTTCTGACGATTCATCCTACTGAGCTTAAATTCCCGTTCGAACTTAAGAAGCAGAGTTCATGTTCTCTTCAGTTGGTAAATAATTCAGATCATTATGTGGCC
TTCAAGGTGAAAACTACATCCCCAAAGAAGTACTGTGTACGACCCAACACTGGTGTTGTGCTGCCTAAGTCTACTTGCGATGTGATAGTCACTATGCAATCACAGCGAGAAGCCCCTCCTGATCTGCAGTGCAAAGACAAATTCCTTGTGCAGAGCGTTGTAGCACCTTATGGGGTAGATCAGAAGGATATCACTCCGGA
AATGTTCATCAAGGACGATGGGAAGGTTGTTGAGGAGTGCAAGCTCAGGGTGGTTTATGCTCCTCCGCCCCAACCTCCTTCTCCAGTTCCTGAAGGATCAGAAGAGGGCTCATCTCCAAGGGCGTTGTCATCCGACAATGGACAACAGAATACTGGTTTGTTCAAAGTGGCACCCAAAGAACTTGGTGAATTGAAGGCAA
AACTTACTGAGGCACGCACAATGCTGTCAAAATTAACAGATGAAAGGAATACAGTTGCGCAGCAAACTCAAGAACTACGTCAGGAATTGGAATCAATGCGCCAAGGAACTCAAAACACCAAATCTCGCTTTTCCATCCTGTTTTGTTTGGTTGTTGCTCTTCTAGGTGTTGCTGCTTGAATACACCATATTTGTTATGAA
TGAATGCTATCGTTGAGCTCCAGCATGTTAAGTGAAGCTCAGACAATTCTATCAGTAGTTGCATTACTGTTGGGTACTCGTGCAGTCTGAACACCTATTTGGTAAGGTTTTGACTGTTCTACAATTCTACGAGTTCAGCATATCATAGAAAGTGTGCTAGGAATGGAATCATGGATGCTTTCTGAAACCAAGCAAGGCTC
GAGTCTCTTCTTGAAGCCAGCCCTGCATCCTTTTAACAGGCTGTATGCTTCTCTTATCGCAAAGGAGGTTGCTTGAAATCATAAATTATTTTGTTCTTTACAATGTTTCTTCTAGGTTTCCTGTATCGTTGAAGGTGCTTTAAAATTTGCCTCTTAGTTTGAGTCTGTTTTGAAGTTAAACATACAGCTATTTATCCCTT
GTGGAAATCTATAATTGAGAATCACTAAGGGTTGCTTGACCTATTATCTGAGTATTTCACTCTTGGTTGTGTTTACTATTCTACATACGTGAAGGTCATAGAATTTGTTATTATGAAATCAATGCACCATGCTGTTGTATAAA

>TRINITY_DN104464_c0_g1_i2 from sample A3 / Example unspliced isoform:

TCTTCTCTTCAACCCCCACGGCATTCCCCACAGCATTTATTACATTGCTCAGTTCATCGAAGCTCAACTCCACCCTCACAGCTCGAAGCCATCCTGTGGAAAAGAAGCATACTGTGCTAATCGGCCAAAGAAATCCTCACTCTCCGATTACAGGCGCTGTGAAACTATTTGAATTCGCGTGCCACTGGAAAACCAGGACC
GACATTGCTAGGGCTGTAAAATTGATTTCGTTTTTTGAGCGCTACAGGAGCGGGGTTCTGGGAGCCTCGAGGTCTGAAAGATCCCAGTTCATTTCTGGTTGCCTCAATTGCTGCTTGGAGGAGTTGATCTGTTCGTAAAATGGGGCTTTGCAAGAGCTGATCTGAAGAGGTTGCTTTGAGGGTTGCGTTTGGCCTGGGCA
TTTGTACCGGAGTTTAAGCTCAAGTTCGGGGTAAAGAATGAGTAACGGCGAGCTTCTGACGATTCATCCTACTGAGCTTAAATTCCCGTTCGAACTTAAGAAGCAGAGTTCATGTTCTCTTCAGTTGGTAAATAATTCAGATCATTATGTGGCCTTCAAGGTGAAAACTACATCCCCAAAGAAGTACTGTGTACGACCCA
ACACTGGTGTTGTGCTGCCTAAGTCTACTTGCGATGTGATAGTCACTATGCAATCACAGCGAGAAGCCCCTCCTGATCTGCAGTGCAAAGACAAATTCCTTGTGCAGAGCGTTGTAGCACCTTATGGGGTAGATCAGAAGGATATCACTCCGGAAATGTTCATCAAGGACGATGGGAAGGTTGTTGAGGAGTGCAAGCTC
AGGGTGGTTTATGCTCCTCCGCCCCAACCTCCTTCTCCAGTTCCTGAAGGATCAGAAGAGGGCTCATCTCCAAGGGCGTTGTCATCCGACAATGGACAACAGAATACTGGTTTGTTCAAAGTGGCACCCAAAGAACTTGGTGAATTGAAGGCAAAACTTACTGAGGCACGCACAATGCTGTCAAAATTAACAGATGAAAG
GAATACAGTTGCGCAGCAAACTCAAGAACTACGTCAGGAATTGGAATCAATGCGCCAAGGAACTCAAAACACCAAATCTCGCTTTTCCATCCTGTTTTGTTTGGTTGTTGCTCTTCTAGgtattctggttgggtaccttgttaatacatagatttgatgtaaccatctgcgttactgttagaaagatcaactgtttgagg
tgcaagggctttgtttgaaagtttttctaaaagcatgaagcatatttggtaacctttgcgttagGTGTTGCTGCTTGAATACACCATATTTGTTATGAATGAATGCTATCGTTGAGCTCCAGCATGTTAAGTGAAGCTCAGACAATTCTATCAGTAGTTGCATTACTGTTGGGTACTCGTGCAGTCTGAACACCTATTTG
GTAAGGTTTTGACTGTTCTACAATTCTACGAGTTCAGCATATCATAGAAAGTGTGCTAGGAATGGAATCATGGATGCTTTCTGAAACCAAGCAAGGCTCGAGTCTCTTCTTGAAGCCAGCCCTGCATCCTTTTAACAGGCTGTATGCTTCTCTTATCGCAAAGGAGGTTGCTTGAAATCATAAATTATTTTGTTCTTTAC
AATGTTTCTTCTAGGTTTCCTGTATCGTTGAAGGTGCTTTAAAATTTGCCTCTTAGTTTGAGTCTGTTTTGAAGTTAAACATACAGCTATTTATCCCTTGTGGAAATCTATAATTGAGAATCACTAAGGGTTGCTTGACCTATTATCTGAGTATTTCACTCTTGGTTGTGTTTACTATTCTACATACGTGAAGGTCATAG
AATTTGTTATTATGAAATCAATGCACCATGCTGTTGTATAAA