sp|P41647|TI214_PINTH ----> Protein TIC 214

Intron 1 GTTTGTTTCT ... TGTTTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.5	170	0	46
A2	16.8	204.5	0	41
A3	20.13	172.5	0	43
A4	28.09	119	2	45
A5	14.29	97	0	16
A6	20.38	1144.5	0	287
A7	19.73	1137	2	274
A8	21.73	1118	0	304
A9	22.16	1177.5	0	328
A10	17.48	923	0	192

>TRINITY_DN47202_c1_g1_i11 from sample A10 / Example spliced isoform

TGAGGTAAGTAGGTGCCCGGAAAGCTTCCTCCTTACAGCTGAGCATTATTCGACGAAAATCAAACCTAGATAATGCGCATGTCAACATAATTCATTTAATTGTCTGGATCTCATTTAATAATTTGGATCTGGAGGCACGGAGTGGAACATTGCCAGGAGAGGAGAGCGGAGGAGGTGAAAAAATGGGGATGATAGGTGAC
ACAGTCGAATCCCTCCGATCGCTTCAGATTAGACAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAG
TAAAGCACCCATTCGTACAGGAGAAATCGTCGTGTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGGGATAACAATTATGGAGATGATAGGCTTTTGTATGCTCAAGGTCAGCTATGGCTTCAGAAGCACCATATAA
TGGGTCGAGCTGTTGGGTTTTTACCGTATGTTGGTTGGGTGACAATCCTTATGACTGAAAAGCCCTTAATCAAGTACATACTGATAGGAGCATTGGGTTTGCTGGTAATAACGTCGAAAGAATAAAGATTATCATTACTTCAGACTCTAGGCATGGAATGCAGGATATAGTTTTTTCGATGGGATGGTTGTGGCACTCTT
GATTTTATACATCAAGAGATTTTGCATCTAAAGGCATATCCTCGGAGATATTTTGTTCTGGACATTTCTTTGATTGTTATGCAAATATTAGATGTCATCCTGTTTCAATGCAGAGTCTGCAAAATGATGGCATTTATCTTGATATTCATTATGCATCTTGTTTAATGCAAATTAATGGAGCTCGGTTTGTGATCGACTCC
TAACTAAATAAAATCACATCAATACAAACAAGTTGCAGCGATGATATAAAAGAAAATAGAGAGATACGATGATCTTAGCAGTCAATTTACAGCAGCATTTTGGGATATAACTAAATGATGCAGAATTTTTAGCTTAAAACCAGCGTGTTACAAAACAAAGATGACAATAATCTCTACAATGAATATGCACTAATATAACC
TTTTGTTTATGGATACATATTGAATGATACATGTAAGCTGATTTTATATATTATAGAATGCTGCAGGG

>TRINITY_DN47202_c1_g1_i1 from sample A10 / Example unspliced isoform:

TGACTGAAAAGCCCTTAATCAAGgtttgtttctgcaacctttttggtcatatcttttaacttagcatgctacacagtttagtttgcatgacaaacaattgagcttaatggcaatactgtgcttagttagttaatgaagtattaaagaacatttgatggagatcagtatgatgtgtgaccattgtattcactttgtagctc
tgtaaacatacatgacatacttccaaattggggtttcaagcagctgtccttgccgtaaactctgtttccaaactattgaacttgtatttggcttttaaggactggatttttctgtatgtgtttactaatgctccctttatttttgttcatgttgtatctctgacattttgaatgatttgtttaccagTACATACTGATAG
GAGCATTGGGTTTGCTGGTAATAACGTCGAAAGAATAAAGATTATCATTACTTCAGACTCTAGGCATGGAATGCAGGATATAGTTTTTTCGATGGGATGGTTGTGGCACTCTTGATTTTATACATCAAGAGATTT

Intron 2 GTGAATAGCA ... GATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	2.31	217.3	0	10
A2	0	204	0	0
A3	1.05	244	0	5
A4	2.67	187.7	5	5
A5	0	96	0	0
A6	0.1	1028	2	0
A7	0	1191	0	0
A8	0.12	890.7	2	0
A9	0.19	1079.7	3	1
A10	0.22	911.7	4	0

>TRINITY_DN127458_c2_g2_i5 from sample A1 / Example spliced isoform

GTTTAATAAAATGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAATCCCACGCTACTGCACGGGACTGCTCAATAGTAGCAC
GGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCGAATCCCTCGGATCGCTCCAGATTAGACAGGTGGCCAT
GCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCACCCATTCGTGCAGGAGAAATCGTTGTGTTTCATGTCG
ATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGTAATTAGATGGCAAAGTTTGAATATGCAGGGATTGAATTGTAAGATCTCTGTATTCAAAATCAGAAGTTCTATTTTGCACCACAGCTTAAGATTTGGAGGATGCATAGTTAGTTA
TTGGTCAGCATTCACCATGTACTGTCAAATTCATTAAGAAAGTTTAGTTGACAACTATTATCAAGTAGATTCTTTGCATCTTTTAGTTCCTGATCAGTTAAGGTGGCTTATGACACTTTGGCCTTCCAATATTTCTTGTTCTTCTGATGATGGAGAATCCCATGTAGATTAGAGTAAGTGGAAATGTTTGTTTTAGACTG
ATTTCAAATTATAATTTTATACAAGGAAGCTTTGTCAGGCAACAGATGGCTTCATGGCTTCTAGCTTTAAGTAGGTGAAAGAGAAACCAGATGTGTGTGCAGTAACTTTCTGGTTGAGCATGCATAGTGAGGCAAGGAAGACCAGTAAGGAGAATATTGAGAAGACTTGTGCCCGTGGTAGGAATTGAAAACAAAAAGGA
GACATGTGCCTAGGAAAGTAGGGTTCTTGAAGAAAGGAATATCAGAGAAAAATAAGCAAGCAGATTTCTCCTGTATAGAAGCTTTTGACTTGTGCATAAAATTTCTTAACATATAAAACAGAAGATAACATATAATGATATATATTAGTACTGGCTGACAAAGGTGGACCTTTCTTCTGTATTCTTTCCTCTTGATGAGT
GTTTGCAAGCCTTAGTTTTATTTTTCAGAATTTTTGGAAGTTTTTTAAATCATTAATATTTATTGGTGCATTTTTGTTTAAGATTTTCCCAAATATTGCACTGAGAGAAGCGAGGTGCACACAATAATATCCCTTTGACCTACTTTAGTGGTCATTCGTTGGACATGAATAATATTATCTTATCTACTGCCAGAGTG

>TRINITY_DN127458_c2_g2_i12 from sample A1 / Example unspliced isoform:

ATAGTTCATCGTGTTATAAAGgtgaatagcatagacaactgcattgccctctgaagtttgatttaggtactgctgaagaaaagaccattgcaatctacaaattgtgtctacaaaaggagggatttttcattttgcatatgtttgtccaaaggggttctcttgactgcattctgaagcaattccagatgacatcacaacat
agtgcagtcttttctttacttcaaatagagggaggaggaagaagcaacatctgttcttatgtacatttatgggtgactgccaagatcttgcttgtcaaccaagaatcaatgtggctaatttttgtctaattttcaatctgtgttgctgtaattgttgaaatgattcttacattcttttgaggttcattaccttatatact
ttgggttgtagcttttgcagttgtaacatgtcagttttgaatattgttttattttgtctgatgttgcagGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGTAATTAGATGGCAAAGTTTGAATATGCAGGGATTGAATTGTAAGATCTCTGTATTCAAAATCAGAAGTTCTATTTTGCACCACA
GCTTAAGATTTGGAGGATGCATAGTTAGTTATTGGTCAGCATTCACCATGTACTGTCAAATTCATTAAGAAAGTTTAGTTGACAACTATTATCAAGTAGATTCTTTGCATCTTTTAGTTCCTGATCAGTTAAGGTGGCTTATGACACTTTGGCCTTCCAATATTTCTTGTTCTTCTGATGATGGAGAATCCCATGTAGAT
TAGAGTAAGTGGAAATGTTTGTTTTAGACTGATTTCAAATTATAATTTTATACAAGGAAGCTTTGTCAGGCAACAGATGGCTTCATG

Intron 3 GTTTTTACCG ... CTTAATCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	91.56	13	153	129
A2	85.78	31	205	169
A3	82.32	35	198	128
A4	80.99	27	133	97
A5	86.8	13	98	73
A6	87.12	152	1140	916
A7	87.52	152	1216	915
A8	86.38	154	1083	871
A9	84.74	191	1203	919
A10	87.14	121	946	694

>TRINITY_DN115679_c0_g4_i11 from sample A3 / Example spliced isoform

GCCCGGAGTTTAATAAAATGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAATCCCACGCTACTGCACGGGACTGCTCAATA
GTAGCACGGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCGAATCCCTCGGATCGCTCCAGATTAGACAGG
TGGCCATGCAGGCCATCAGCCTCGGGTGATATTTTGTTTTTGCATATGAGTAAAGCACCCATTCGTGCAGGAGAAATCGTTGTGTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGAAACTGGAGAGGTTGAAGTCCTTACTAAAGGGGATAACAACGATGGAGATGA
TAGGATTTTGTATGCTCAAGGTCAGCTATGGCTTGAGAAGCACCATATAATGGGTCGAGCTGTTGGTACATACTCGTTGGAGCATTGGGTTTGCTGGTAATAAC

>TRINITY_DN115679_c0_g4_i4 from sample A3 / Example unspliced isoform:

GCCCGGAGTTTAATAAAATGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAATCCCACGCTACTGCACGGGACTGCTCAATA
GTAGCACGGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCGAATCCCTCGGATCGCTCCAGATTAGACAGG
TGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATTTTGTTTTTGCATATGAGTAAAGCACCCATTCGTGCAGGAGAAATCGTTGTGTTT
CATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGAAACTGGAGAGGTTGAAGTCCTTACTAAAGGGGATAACAACGATGGAGATGATAGGATTTTGTATGCTCAAGGTCAGCTATGGCTTGAGAAGCACCATATAATGGGTCGAGCTGTTGGgtttttaccgtatgttggctg
ggtgacaatccttatgactgaaaaacccttaatcaagTACATACTCGTTGGAGCATTGGGTTTGCTGGTAATAACATCAAAAGAATAGAGATTAGCAATACTTCAGACTCTATTCTCCATGCATCTCATTGTAACTTCAAGGTTATTTTTGTGAACAGAGGCATGGAATGCAGGATAGTTTTTTCGAGGGGATGGTTGTG
GCACTCTTGATTTTATACATCAAGAGATTTGACATCTAAAGGCATATCCTCTGAGATATTTTGTTCTGGGCATTTCTTTGATTGTTATGCAAATATTAGATGTTATCCTGTTTCAATCCAGAGGCTGCAAAATGAAGGCATTTATCTTGATATTCATTTTGTATCTTGTTTTATGCAAATTAATGGAGTTTGGTTTGTGA
TCTTAGCTATCAAGTAGGGACTCTTAACTAAATAAAATCATATCAATACAAACACGTTGCAGTGATGATATAAAACAAATCAACTTGTAAAACAATGTTTATAAAAGAAAATAGAGAGATACGATGATCTTAGCAGTCAATTTACAGCAGCATTTTGGGATATAACTAAATGATGCAGAATTTTTAGCTTAAAACCAGCG
TGTTACAAAACAAAGATGACAATAATCTCTACAATGAATATGCACTAATATAACCTTTTGTTTATGGAG

Intron 4 GTAAAACTAT ... CTTTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	5.26	153	3	8.5
A2	2.52	213	0	5.5
A3	1.49	199	0	3
A4	4.38	131	0	6
A5	1.42	104	1	1.5
A6	0.29	1175	0	3.5
A7	0.2	1248	0	2.5
A8	0.53	1122	2	6
A9	0.45	1231	2	5.5
A10	0.47	962	0	4.5

>TRINITY_DN44995_c1_g1_i5 from sample A8 / Example spliced isoform

CAGAGCCCGGAGTTTAATAAAAAGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAATCCCACGCTACTGCACGGGACTGCTC
AATAGTAGCACGGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCGAATCCCTCGGATCGCTCCAGATTAGA
CAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCACCCATTCGTACAGGAGAAATCGTCGT
GTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGGGATAACAATTATGGAGATGATAGGCTTTTGTATGCTCAAGGTCAGCTATGGCTTCAGAAGCACCATATAATGGGTCGAGCTGTTGGGTTTTTACCGTATGTTG
GCTGGGTGACAATCCTTATGACTGAAAAGCCCTTAATCAAGTACATACTGATAGGAGCATTGGGTTTGCTGGTAATAACGTCGAAAGAATAAAGATTATCATTACTTCAGACTCTAGGCATGGAATGCAGGATATAGTTTTTTCGATGGGATGGTTGTGGCACTCTTGATTTTATACATCAAGAGATTTTGCATCTAAAG
GCATATCCTCGGAGATATTTTGTTCTGGACATTTCTTTGATTGTTATGCAAATATTAGATGTCATCCTGTTTCAATGCAGAGTCTGCAAAATGATGGCATTTATCTTGATATTCATTATGCATCTTGTTTAATGCAAATTAATGGAGCTCGGTTTGTGATCGACTCCTAACTAAATAAAATCACATCAATACAAACAAGT
TGCAGCGATGATATAAAAGAAAATAGAGAGATACGATGATCTTAGCAGTCAATTTACAGCAGCATTTTGGGATATAACTAAATGATGCAGAATTTTTAGCTTAAAACCAGCGTGTTACAAAACAAAGATGACAATAATCTCTACAATGAATATGCACTAATATAACCTTTTGTTTATGGAGACATTGAATGATACATGTA
AGCTGATTTTATATGTTATAGAATGCTGCAG

>TRINITY_DN44995_c1_g1_i4 from sample A8 / Example unspliced isoform:

AATGGGTCGAGCTGTTGGgtaaaactatctttcacagattaagctctcccatgattataaaaggaagtaatgacaaatgattaatcagtggaaaggaaaactggagttttaaaagcagatacatgttgtttctgctcttttagctcatatatgcagctgctttcttttatgtcgtgttggaagaaagtagcttgtttact
tccattctggtttcctctttccatggtgtgtctggaaagaaaaattcccgaatttgcagtgaaagtctttttcatcctttataaattctgttggatattggaagagtaattctgttggatattggaagagtttgaaatgaatttgtctatgataggcttcattttttgatattctccttatacttgtttgtctccatata
tattaatatttaaatggaactccaaagaatgatttgatggcctttgtgcagGTTTTTACCGTATGTTGGCTGGGTGACAATCCTTATGACTGAAAAGCCCTTAATCAAGTACATACTGATAGGAGCATTGGGTTTGCTGGTAATAAC

Intron 5 GTAAATCATG ... GCCATGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.27	12	7	2
A2	27.27	8	4	2
A3	73.33	2	7	4
A4	33.33	4	3	1
A5	40	3	4	0
A6	35.14	12	9	4
A7	28	27	14	7
A8	29.41	36	18	12
A9	23.91	35	20	2
A10	34.38	21	19	3

>TRINITY_DN47202_c1_g1_i2 from sample A10 / Example spliced isoform

CAGATTAGACAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCACCCATTCGTGCAGGAGA
AATCGTTGTGTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGAGAATAAGACTTTCTTCATCTGCACCTGGCACAGTACATAAAAGAAGACAGATTCTCTCATAGATTTAATTCTCAGATCTTCTGATAACCAGCCTTGGATTGAAAACACCAGAAGCAAATTGGCCATCATGATGTACATATT
TCTAGTATGGAGCCAAGGGCCGAGGCCTGGCTAGAGAATGGTGGGACTTGGACTTTGGTTTATGCCATATTTTATATTTCCTAGGTACTATGTAGTATGTAGTATGTAGTAATGTCACAGTGGTACACCATAACTTATGTTCAAAAGCTAAAATGGTGAAATACTATAATGTAATGATCGTACTAATAAATGACGTTAAT
TCCTTTCTAAGTGTCAAGTTAAGAACTTGCCACCTTTTTACCTTGTGCATTAAGTTATATTACCTTGTCAATGTCTTATACGCTCGAAGAGGAC

>TRINITY_DN47202_c1_g1_i5 from sample A10 / Example unspliced isoform:

CAGATTAGACAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCACCCATTCGTGCAGGAGA
AATCGTTGTGTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGAGAATAAGACTTTCTTCATCTGCACCTGGCACAGTACATAAAAGAAGACAGATTCTCTCATAGATTTAATTCTCAGATCTTCTGATAACCAGgtaaatcatgtcctaaaagttgatgatttcagagtatttcacagtaaagt
gctgatattcatacagacatattacagtagtccagaattaaaagacaaatcaagatacatatagtctatgacactaccttcaattgtttacataatttatatgtaataatttgtatactggacttttgatcaaagtgttatgccatgtcagCCTTGGATTGAAAACACCAGAAGCAAATTGGCCATCATGATGTACATAT
TTCTAGTATGGAGCCAAGGGCCGAGGCCTGGCTAGAGAATGGTGGGACTTGGACTTTGGTTTATGCCATATTTTATATTTCCTAGGTACTATGTAGTATGTAGTATGTAGTAATGTCACAGTGGTACACCATAACTTATGTTCAAAAGCTAAAATGGTGAAATACTATAATGTAATGATCGTACTAATAAATGACGTTAA
TTCCTTTCTAAGTGTCAAGTTAAGAACTTGCCACCTTTTTACCTTGTGCATTAAGTTATATTACCTTGTCAATGTCTTATACGCTCGAAGAGGAC

Intron 6 GTGAATAGCA ... GATGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.63	210	0	41
A2	10.61	197.8	0	23
A3	19.7	234.8	0	56
A4	18.71	181.8	5	41
A5	9.08	92.5	0	9
A6	11.87	994.5	2	131
A7	12.63	1155.5	0	164
A8	13.12	860.5	2	127
A9	13.31	1043.8	3	157
A10	10.45	887.8	4	102

>TRINITY_DN127458_c2_g2_i5 from sample A1 / Example spliced isoform

GTTTAATAAAATGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAATCCCACGCTACTGCACGGGACTGCTCAATAGTAGCAC
GGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCGAATCCCTCGGATCGCTCCAGATTAGACAGGTGGCCAT
GCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCACCCATTCGTGCAGGAGAAATCGTTGTGTTTCATGTCG
ATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGTAATTAGATGGCAAAGTTTGAATATGCAGGGATTGAATTGTAAGATCTCTGTATTCAAAATCAGAAGTTCTATTTTGCACCACAGCTTAAGATTTGGAGGATGCATAGTTAGTTA
TTGGTCAGCATTCACCATGTACTGTCAAATTCATTAAGAAAGTTTAGTTGACAACTATTATCAAGTAGATTCTTTGCATCTTTTAGTTCCTGATCAGTTAAGGTGGCTTATGACACTTTGGCCTTCCAATATTTCTTGTTCTTCTGATGATGGAGAATCCCATGTAGATTAGAGTAAGTGGAAATGTTTGTTTTAGACTG
ATTTCAAATTATAATTTTATACAAGGAAGCTTTGTCAGGCAACAGATGGCTTCATGGCTTCTAGCTTTAAGTAGGTGAAAGAGAAACCAGATGTGTGTGCAGTAACTTTCTGGTTGAGCATGCATAGTGAGGCAAGGAAGACCAGTAAGGAGAATATTGAGAAGACTTGTGCCCGTGGTAGGAATTGAAAACAAAAAGGA
GACATGTGCCTAGGAAAGTAGGGTTCTTGAAGAAAGGAATATCAGAGAAAAATAAGCAAGCAGATTTCTCCTGTATAGAAGCTTTTGACTTGTGCATAAAATTTCTTAACATATAAAACAGAAGATAACATATAATGATATATATTAGTACTGGCTGACAAAGGTGGACCTTTCTTCTGTATTCTTTCCTCTTGATGAGT
GTTTGCAAGCCTTAGTTTTATTTTTCAGAATTTTTGGAAGTTTTTTAAATCATTAATATTTATTGGTGCATTTTTGTTTAAGATTTTCCCAAATATTGCACTGAGAGAAGCGAGGTGCACACAATAATATCCCTTTGACCTACTTTAGTGGTCATTCGTTGGACATGAATAATATTATCTTATCTACTGCCAGAGTG

>TRINITY_DN127458_c2_g2_i3 from sample A1 / Example unspliced isoform:

ATAGTTCATCGTGTTATAAAGgtgaatagcatagacaactgcattgccctctgaagtttgatttaggtactgctgaagaaaagaccattgcaatctacaaattgtgtctacaaaaggagggatttttcattttgcatatgtttgtccaaaggggttctcttgactgcattctgaagcaattccagatgacatcacaacat
agtgcagtcttttctttacttcaaatagagggaggaggaagaagcaacatctgttcttatgtacatttatgggtgactgccaagatcttgcttgtcaaccaagaatcaatgtggctaatttttgtctaattttcaatttatgtgttgctgtaattgttatgtactatggacctgcttggttgaagtgatgcttacattct
tttgaggttccttaccttagatactttggattgtacttttgcagttgtaacattacagttatgaagattgttttattttgtctgatgttgcagGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGGGATAACAATTATGGAGATGATAGGCTTTTGTATGCTCAAGGTCAGCTATGGCTTGAGAA
GCACCATATAATGGGTCGAGCTGTTGGGTTTTTACCGTATGTTGGTTGGGTGACAATCCTTATGACTGAAAAGCCCTTAATCAAGTACATACTGATAGGAGCATTGGGTTTGCTGGTAATAACGTCGAAAGAATAAAGATTATCATTACTTCAGACTCTAGGCATGGAATGCAGGATAGTTTTTTCGATGGGATGGTTGT
GGCACTCTTGATTTTATACATCAAGAGATTTTGCATCTAAAGGCATATCCTCGGAGATATTTTGTTCTGGACATTTCTTTGATTGTTATGCAAATATTAGATGT

Intron 7 GTTTGTTTCT ... TGTTTACCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	43.71	127.3	5	46
A2	40.68	150.7	5	41
A3	44.11	128	0	43
A4	47.95	95.3	7	45
A5	41.93	72	1	16
A6	44.79	880.7	1	287
A7	44.26	875.7	3	274
A8	45.49	863.7	2	304
A9	44.63	914	2	328
A10	41.86	701.7	0	192

>TRINITY_DN44995_c1_g1_i5 from sample A8 / Example spliced isoform

CAGAGCCCGGAGTTTAATAAAAAGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAATCCCACGCTACTGCACGGGACTGCTC
AATAGTAGCACGGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCGAATCCCTCGGATCGCTCCAGATTAGA
CAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCACCCATTCGTACAGGAGAAATCGTCGT
GTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGTACATGAACGGCAAGACACTGGTGAGGTTGAAGTCCTTACTAAAGGGGATAACAATTATGGAGATGATAGGCTTTTGTATGCTCAAGGTCAGCTATGGCTTCAGAAGCACCATATAATGGGTCGAGCTGTTGGGTTTTTACCGTATGTTG
GCTGGGTGACAATCCTTATGACTGAAAAGCCCTTAATCAAGTACATACTGATAGGAGCATTGGGTTTGCTGGTAATAACGTCGAAAGAATAAAGATTATCATTACTTCAGACTCTAGGCATGGAATGCAGGATATAGTTTTTTCGATGGGATGGTTGTGGCACTCTTGATTTTATACATCAAGAGATTTTGCATCTAAAG
GCATATCCTCGGAGATATTTTGTTCTGGACATTTCTTTGATTGTTATGCAAATATTAGATGTCATCCTGTTTCAATGCAGAGTCTGCAAAATGATGGCATTTATCTTGATATTCATTATGCATCTTGTTTAATGCAAATTAATGGAGCTCGGTTTGTGATCGACTCCTAACTAAATAAAATCACATCAATACAAACAAGT
TGCAGCGATGATATAAAAGAAAATAGAGAGATACGATGATCTTAGCAGTCAATTTACAGCAGCATTTTGGGATATAACTAAATGATGCAGAATTTTTAGCTTAAAACCAGCGTGTTACAAAACAAAGATGACAATAATCTCTACAATGAATATGCACTAATATAACCTTTTGTTTATGGAGACATTGAATGATACATGTA
AGCTGATTTTATATGTTATAGAATGCTGCAG

>TRINITY_DN44995_c1_g1_i3 from sample A8 / Example unspliced isoform:

AATGGGTCGAGCTGTTGGGTAAAACTATCTTTCACAGATTAAGCTCTCCCATGATTATAAAAGGAAGTAATGACAAATGATTAATCAGTGGAAAGGAAAACTGGAGTTTTAAAAGCAGATACATGTTGTTTCTGCTCTTTTAGCTCATATATGCAGCTGCTTTCTTTTATGTCGTGTTGGAAGAAAGTAGCTTGTTTACT
TCCATTCTGGTTTCCTCTTTCCATGGTGTGTCTGGAAAGAAAAATTCCCGAATTTGCAGTGAAAGTCTTTTTCATCCTTTATAAATTCTGTTGGATATTGGAAGAGTAATTCTGTTGGATATTGGAAGAGTTTGAAATGAATTTGTCTATGATAGGCTTCATTTTTTGATATTCTCCTTATACTTGTTTGTCTCCATATA
TATTAATATTTAAATGGAACTCCAAAGAATGATTTGATGGCCTTTGTGCAGGTTTTTACCGTATGTTGGCTGGGTGACAATCCTTATGACTGAAAAGCCCTTAATCAAGgtttgtttctgcaacctttttggtcattttttttaacttagcatgctacacagttagtttgcatgacaaacaattgagcttaatggcaatc
ctgtgcttagttagttaatgaagtattaaagaacatttgatggagatcagtatgatgtgtgaccattgtattcactttgtagctctgtaaacatacatgacatacttccaaattggggtttcaagcagctgtccttgccgtaaactctgtttccaaactattgaacttgtatttggcttttaaggactggatttttctgt
atgtgtttactaatgctccctttatttttgttcatgttgtatctctgacattttgaatgatttgtttaccagTACATACTGATAGGAGCATTGGGTTTGCTGGTAATAACGTCGAAAGAATAAAGATTATCATTACTTCAGACTCTAGGCATGGAATGCAGGATATAGTTTTTTCGATGGGATGGTTGTGGCACTCTTGA
TTTTATACATCAAGAGATTT

Intron 8 GTACATGAAC ... CTTACTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	75.66	83	275	241
A2	72.66	92	253	236
A3	73.19	115	318	310
A4	72.85	79	237	187
A5	70.35	47	124	99
A6	67.6	587	1295	1155
A7	68.45	657	1475	1376
A8	68.91	494	1133	1057
A9	70.68	551	1370	1286
A10	65.96	529	1104	946

>TRINITY_DN47202_c1_g1_i6 from sample A10 / Example spliced isoform

CGGAAGAGAATATTCCTCAGGGGGATGTTCCGAGAAAGGTAAGCAATGGAAATGACATATTGGAAGAGAATCTTCTTCAAGAGGATGTTCCGAAAAAGGTAAGCAATAGAAATGAAACATCGGTAAATAAAAAAATATTGGTCCCCGAACCATATCGTTTATCAAGCATACCGGATGACGAATTCCTGATGTATAAAATC
GTCAGTATTTCATTGAACTTTCAAAACAGAGCCCGGAGTTTAATAAAAAGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAA
TCCCACGCTACTGCACGGGACTGCTCAATAGTAGCACGGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCG
AATCCCTCGGATCGCTCCAGATTAGACAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCA
CCCATTCGTGCAGGAGAAATCGTTGTGTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGGGGATAACAACGATGGAGATGATAGGATTTTGTATGCTCAAGGTCAGCTATGGCTTCAGAAGCACCATATAATGGGTCGAGCTGTTGGGTTTTTACCGTATGTTGGTTGGGTGACAATCCTTATG
ACTGAAAA

>TRINITY_DN47202_c1_g1_i8 from sample A10 / Example unspliced isoform:

CGGAAGAGAATATTCCTCAGGGGGATGTTCCGAGAAAGGTAAGCAATGGAAATGACATATTGGAAGAGAATCTTCTTCAAGAGGATGTTCCGAAAAAGGTAAGCAATAGAAATGAAACATCGGTAAATAAAAAAATATTGGTCCCCGAACCATATCGTTTATCAAGCATACCGGATGACGAATTCCTGATGTATAAAATC
GTCAGTATTTCATTGAACTTTCAAAACAGAGCCCGGAGTTTAATAAAAAGCAACAAAGAACGTACCACCAATAGCAGGGTAGGGACAAGGGCAAAGGCGGGGGCTTCATCTGTCTACCAAGAAGCTGGTCGTCTTGATCTGCTTCAGGTCGTCGGCGGGTTTTCATGTAACGCGCATGTCAACACACTGCCAGGACCTAA
TCCCACGCTACTGCACGGGACTGCTCAATAGTAGCACGGAAATAACTGCCTTTCTTTCGCTTCGCTGCTAAAGCGTAATTACATAAGACCCGCATAGTTCATTTAATTGTTTGGATCTGGAGGCACGGAGTGGAACATTGCCGAGAGAGGAGTGGAGAGCTGAGGAGGTTAAAAAATGCGGATGATAGGTGACACAATCG
AATCCCTCGGATCGCTCCAGATTAGACAGGTGGCCATGCAGGCCATCAGCCTCGGAATGATTGTTACATCAGCTTTGATAATATGGAAAGGGCTGATGTGCGTCACAGGAAGTGAATCACCAGTTGTAGTTGTGCTTTCAGGTAGCATGGAACCTGGATTTAAAAGGGGTGATATATTGTTTTTGCATATGAGTAAAGCA
CCCATTCGTGCAGGAGAAATCGTTGTGTTTCATGTCGATGGTCGAGAAATTCCTATAGTTCATCGTGTTATAAAGgtacatgaacggcaagaaactggagaggttgaagtccttactaaagGGGATAACAACGATGGAGATGATAGGATTTTGTATGCTCAAGGTCAGCTATGGCTTCAGAAGCACCATATAATGGGTCG
AGCTGTTGGGTTTTTACCGTATGTTGGTTGGGTGACAATCCTTATGACTGAAAAACCCTTAATCAAGTACATACTCGTTGGAGCATTGGGTTTGCTGGTAATAACATCAAAAGAATAGAGATTAGCAATACTTCAGACTCTATTCTCCATGCATCTCATTGTAACTTCAAGGTTATTTTTGTGAACAGAGGCATGGAATG
CAGGATAGTTTTTTCGAGGGGATGGTTGTGGCACTCTTGATTTTATACATCAAGAGATTTGACATCTAAAGGCATATCCTCTGAGATATTTTGTTCTGGGCATTTCTTTGATTGTTATGCAAATATTAGATGTTATCCTGTTTCAATCCAGAGGCTGCAAAATGAAGGCATTTATCTTGATATTCATTATGTATCTTGTT
TTATGCAAATTAATGGAGTTTGGTTTGTGATCTTAGCTATCAAGTAGGGACTCTTAACTAAATAAAATCATATCAATACAAACACGTTGCAGTGATGATATAAAACAAATCAACTTGTAAAACAATGTTTATAAAAGAAAATAGAGAGATACGATGATCTTAGCAGTCAATTTACAGCAGCATTTTGGGATATAACTAAA
TGATGCAGAATTTTTAGCTTAAAACCAGCGTGTTACAAAACAAAGATGACAATAATCTCTACAATGAATATGCACTAATATAACCTTTTGTTTATGGATACATATTGAATGATACATGTAAGCTGATTTTATATATTATAGAATGCTGCAGGG