sp|Q9LVJ1|SBT14_ARATH ----> Subtilisin-like protease SBT1.4

Intron 1 GTGTGTTTGA ... AATGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.09	28	15	39
A2	68.63	8	19	16
A3	49.43	22	19	24
A4	56.1	9	12	11
A5	65.85	7	11	16
A6	56.25	49	46	80
A7	53.09	57	53	76
A8	59	49	57	84
A9	51.97	55	46	73
A10	43.12	31	22	25

>TRINITY_DN125426_c1_g5_i3 from sample A2 / Example spliced isoform

GAGTCTCTAGTGATCATTGCTTTCTTGCTTTACTGCTTCCTATGACGCGTTGGCTGCGAGTCTCTAGTGATCATTGCTTTCTTGCTTTACTGCTTCCTATGACGCGTTGGCTGCGAGTCTCTAGTGATCATTGCTTTATTGCTTTCTTGCTTTACTGCTATAGAGAATATGGATGGATGCCTGGCATTATTTCCAGATTC
CGTCAATGCCATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCGAAAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGCGTCAACATCGGTCCAGTTTATTTTGGTTTCAATTCTGTTGGT
TTCAATTCTGAAAACATTGCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCCGGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACCAT
TCAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAACCATTGCTCAGACCCAACTCGGC

>TRINITY_DN125426_c1_g5_i1 from sample A2 / Example unspliced isoform:

GAATTTTCAATGCTTCTTGCAGGTCTTTTCCAAGGCTTCCTATGCACTCGCTCTAGTTCAGAGATTTACTGCTACCGATGCCCAGTTCAGACGCGTTGGATGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCG
AAAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGCGTCAACATCGGTCCAGTTTATTTTGGTTTCAATTCTGTTGGTTTCAATTCTGAAAACATTGgtgtgtttgaatgatgattatcgaattctattggtttgaattatgaaaacattattgaattatgaaaaca
ttattggtttgaatgatgcagCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCCGGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACC
ATTCAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAACCATTGCTCAGACCCAACTCGGC

Intron 2 ATCTCATGAT ... CAGATCCTAC

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.59	60	34	48
A2	24.14	55	21	14
A3	28.12	69	29	25
A4	44.44	25	22	18
A5	37.37	31	15	22
A6	61.9	56	90	92
A7	45.39	74	52	71
A8	45.45	48	31	49
A9	41.62	54	39	38
A10	58.41	47	58	74

>TRINITY_DN123059_c0_g1_i6 from sample A3 / Example spliced isoform

GAAAAAGCGGGCTTCAAGCTCAGTATAGTACATCACCAATTGTATTTTCTCAAAACCTTGCATGTCCGCGAAAGGAATTTGAAGACGGGGAGATCCAGATCAGGGAGATAAATTGAACTTTCAATGCTTCTTGCGAGTCTTTTCCCAAGGCTTCCTGTGCACTCGCTCTAGTGATCATTGCTTTACTGCTACCGATGCCC
AGCAGTTCAGACGCGTTGGCTGCATCTCCACGAACACCGGTACTTGATAGACCAAGTTTCTGGCTCCAAATCGGATCGCGCTGCTATGCTTTATACGTACGATACAGTAACGCACGGCTTTGTTGCCAAGCTCACAAGCATGGAGGCTCAAGCTATTTCCTGGCACTATTTCCAGATTTCATCAATCAGGTCGAACCCGG
GACTTTCTGGGAATGAGATCTAGTTATTGTCTATGGCAAGAGCTTCAGCGATCATGGCCTTACGCTGCCAGCTTCCAGATGGAAGGGAGAATGTGAAAGCCGACGGACTCACCGTGGTGGGTAAAGTTAAAGTACGAGCTCTGGTGGTAGCTGGATTCTCGTCCAGAGAGCCGAATCTGGTCGCTCCGGAGATCCTTCAA
CAGGACCTGGTTGCTCCCGACGTCAACATAGCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCTTCCGGTGATAGACCCAACTGGCGGTCCTTGATCTATCTCTAATTGGCTTGCAGAACATTCTGAATCTGGCGAATGAAGGATTGCTGAAACCATTGCTCGAATTACAAACGAATCCGACG

>TRINITY_DN123059_c0_g1_i1 from sample A3 / Example unspliced isoform:

TTTCAATGCTTCTTGCGAGTCTTTTCCCAAGGCTTCCTGTGCACTCGCTCTAGTGATCATTGCTTTACTTCTACCGATGCCCAGCAGTTCAGACGCGTTGGCTGCatctcatgatgattaggctaccagatcctacATCTCCACGAACACCGGTACTTGATAGACCAAGTTTCTGGCTCCAAATCGGATCGCGCTGCTAT
GCTTTATACGTACGATACAGTAACGCACGGCTTTGGTGCCAAGCTCACCAGCATGGAGGCTCAAGCTATTTCCTGGCACTATTTCCAGATTTCATCAATCAGGTCGAACCCGGGACTTTCTGGGAATGAGATCTAGTTATTGTCTATGGCAAGAGCTTCAGCGATCATGGCCTTACGCTGCCAGCTTCCAGATGGAAGGG
AGAATGTGAAAGCCGACGGACTCACCGTGGTGGGTAAAGTTAAAGTACGAGCTCTGGTGGTAGCTGGATTCTCGTCCAGAGAGCCGAATCTGGTCGCTCCGGAGATCCTTCAACAGGACCTGGTTGCTCCCGACGTCAACATAGCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCTTCCGGTGATAGACCCAACTG
GCGGTCCTTGATCTATCTCTAATTGGCTTGCAGAACATTCTGAATCTGGCGAATGAAGGATTGCTGAAACCATTGCTCGAATTACAAACGAATCCGACG

Intron 3 GTCCAGTTTA ... AATGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	42.5	46	19	49
A2	45.45	27	25	20
A3	41.1	48	39	28
A4	50.72	17	16	19
A5	56.1	9	6	17
A6	36.71	150	77	97
A7	38.53	142	95	83
A8	41.97	121	86	89
A9	35.37	159	93	81
A10	34.62	68	45	27

>TRINITY_DN46722_c1_g1_i14 from sample A8 / Example spliced isoform

ATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGACGTTACACATCGCTTTCAGTATGGAAAAAAGCGGGCTTCAAGCTC
AGTATAGTACATCACATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGATGTTACACATCGCTTTCAGTATGGGAAAAA
GCGGGCTTCAAGATCCAGATCAGGGAGATAAATTGAATTTTCAATGCTTCTTGCAGGTCTTTTCCCAAGGCTTCCTATGCCCAGTTCAGACGCGTTGGCTGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCGA
AAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGTGTCAACATCGCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCCGGCGAATGAAGGATT
GCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACCATTCAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAATTATACCAACCT
GAGTATAAGATTTGTAT

>TRINITY_DN46722_c1_g1_i20 from sample A8 / Example unspliced isoform:

ATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGACGTTACACATCGCTTTCAGTATGGAAAAAAGCGGGCTTCAAGCTC
AGTATAGTACATCACATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGATGTTACACATCGCTTTCAGTATGGGAAAAA
GCGGGCTTCAAGATCCAGATCAGGGAGATAAATTGAATTTTCAATGCTTCTTGCAGGTCTTTTCCCAAGGCTTCCTATGCCCAGTTCAGACGCGTTGGCTGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCGA
AAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGTGTCAACATCGgtccagtttattttggtttgaattctgttggtttgaattctgaaaacattggtgtgcttcaattatgattatcgaattctattggtttgaattatgaaaacattattgaattaggttggttt
gaattctgaatgatgcagCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCCGGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACCATT
CAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAATTATACCAACCTGAGTATAAGATTTGTAT

Intron 4 GTTTCAATTC ... AATGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	47.9	31	18	39
A2	47.95	19	19	16
A3	44.14	31	25	24
A4	42.31	15	11	11
A5	56.52	10	10	16
A6	38.04	123	71	80
A7	39.09	120	78	76
A8	41.52	119	85	84
A9	35.63	131	72	73
A10	34.91	55	34	25

>TRINITY_DN125426_c1_g5_i5 from sample A2 / Example spliced isoform

GAATTTTCAATGCTTCTTGCAGGTCTTTTCCAAGGCTTCCTATGCACTCGCTCTAGTTCAGAGATTTACTGCTACCGATGCCCAGTTCAGACGCGTTGGATGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCG
AAAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGCGTCAACATCGGTCCAGTTTATTTTGCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATC
CGGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACCATTCAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAACCATTGCTCAGACCCAACTCGGC

>TRINITY_DN125426_c1_g5_i1 from sample A2 / Example unspliced isoform:

GAATTTTCAATGCTTCTTGCAGGTCTTTTCCAAGGCTTCCTATGCACTCGCTCTAGTTCAGAGATTTACTGCTACCGATGCCCAGTTCAGACGCGTTGGATGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCG
AAAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGCGTCAACATCGGTCCAGTTTATTTTGgtttcaattctgttggtttcaattctgaaaacattggtgtgtttgaatgatgattatcgaattctattggtttgaattatgaaaacattattgaattatgaaaaca
ttattggtttgaatgatgcagCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCCGGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACC
ATTCAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAACCATTGCTCAGACCCAACTCGGC

Intron 5 GTTTGAATTC ... AATGATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.67	31	20	49
A2	51.9	19	21	20
A3	47.01	31	27	28
A4	50	15	11	19
A5	52.94	12	10	17
A6	40.74	124	74	96.5
A7	40.39	121	81	83
A8	42.38	121	89	89
A9	37.05	130	72	81
A10	36.05	55	35	27

>TRINITY_DN46722_c1_g1_i17 from sample A8 / Example spliced isoform

ATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGACGTTACACATCGCTTTCAGTATGGAAAAAAGCGGGCTTCAAGCTC
AGTATAGTACATCACATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGATGTTACACATCGCTTTCAGTATGGGAAAAA
GCGGGCTTCAAGATCCAGATCAGGGAGATAAATTGAATTTTCAATGCTTCTTGCAGGTCTTTTCCCAAGGCTTCCTATGCCCAGTTCAGACGCGTTGGCTGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCGA
AAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGTGTCAACATCGGTCCAGTTTATTTTGCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCC
GGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACCATTCAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAA
GAATTATACCAACCTGAGTATAAGATTTGTAT

>TRINITY_DN46722_c1_g1_i20 from sample A8 / Example unspliced isoform:

ATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGACGTTACACATCGCTTTCAGTATGGAAAAAAGCGGGCTTCAAGCTC
AGTATAGTACATCACATTTGGAGTACGCGGATATAGGTGTCTGTTTTCTTGAGAGAGTGAGATTTACCAAACACACCATCATGGATCGATGGAACCTGGAGGCGTTCGCTAGCTACTTTCTTAAGAACATTCTATAAATGTTCAAATCCCGATGTGTTGACGTGTAAGGATGTTACACATCGCTTTCAGTATGGGAAAAA
GCGGGCTTCAAGATCCAGATCAGGGAGATAAATTGAATTTTCAATGCTTCTTGCAGGTCTTTTCCCAAGGCTTCCTATGCCCAGTTCAGACGCGTTGGCTGCATCTCATGATGATCAGGGATTGAGCTCTAGTTATGGTCTATGGCCACTGTCACATTACGGTGATGATGTTATCGTGGGCGCGCTGGATTATGGCCCGA
AAGCAAGAGCTTCAGCGATGATCGCCTTACGCCTGGAGATCCTTAAACAGGACCTGGTTGCTCCGGGTGTCAACATCGGTCCAGTTTATTTTGgtttgaattctgttggtttgaattctgaaaacattggtgtgcttcaattatgattatcgaattctattggtttgaattatgaaaacattattgaattaggttggttt
gaattctgaatgatgcagCTGGGAGCCAACCAGACCCAACTCGGCTTTCAGCCCTCCCGTGATAGACCCAATTGGCGGTCGTGAATCTATCTCCAATTGGCTTGCCGAAACCATTGCTCGAATCCGGCGAATGAAGGATTGCCGAAACCATTGTTCGAATTACAGAGGAATCCGGCGAAAGAAGGATCGCTGAAACCATT
CAAACTAAGGATTGTTGAGCCTCCAGTATCAGTCCGAATCCGGCGAACAAGAAGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAGGTGCTGAAGCCATTGCTCAGAAGAATTATACCAACCTGAGTATAAGATTTGTAT