NA ----> NA

Intron 1 GTCTGAGGTG ... TGTTTGTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.88	3.6	2	3
A2	84.85	1.8	9	11
A3	67.74	5	13	8
A4	60	2	4	2
A5	68	0.8	1	2
A6	63.2	7.6	13	13
A7	54.76	22.2	26	27
A8	77.14	9	27	33
A9	56.13	20.8	25	27
A10	49.5	31.2	28	32

>TRINITY_DN43211_c1_g1_i7 from sample A7 / Example spliced isoform

ATCAAAGGCTTCAAGATCTTCAAGTCCAAAACCTTTCCTTTCTTTTCTTATATGATAAACATTGTGGACGGATTATCGGGCAATTAACTAATATCTCTTCGCGTGTCCATGCACAGAATGAGAAAGAGTTACGAACAAGCAAACTTTCACTTTTGTCGGAGAAAATACTGGACTTAAGGCGTATTCGACATCGTTGCTTG
AAGCTGGAGCAAAGAGGGCCCGAATTGAGCTTGCAAATTTTGATGAAAGAAGACGAAATACGGAGTATTGAAGCCAAAAATGAGCAGCTGAACTCAAAGCTCAGGGAAGTAACTCGCGAGGTTTACGAGGCAGATGAAGAGAGAAACCAGAATCAGAAATATTATGAGCTTCAGAAACAGGAAATGCAAAAGTTCGTGCA
AGAGTTTGAACAGCGTTGGTCAATCTTAAGCTCGAATCAGCAGTGAGAGAAGTGCACAATACCAACAGATCTGTCTCCAGAAATGACGGTGAGAGTGCTTTTCAGTTTGTTGGTAGAGCAAAACTCTTCACAACAGAAAGGGAGCTTCCTAGCAGCTTGTCCTCTAGCCACTCGTTGAGAAGCTTTTTAGAGGCACAACT
GGAGAGTACTCTTATTTCTCAAGATGAAGAGAGGAAGAAACTTTTGAAAGTTATGCAAAATCAAATAATTGACAATGGGCTTTTGCAAAGAGAGATCCAGGACCTTGAAGAAAAAGTGTTTGAAGTGATGAGAAGGCGAGATGAAAGGAAAAAGTCAGTTCTATCTTATCTGTACTTTTACTATTCCAACTCTTTTATCA
TCACATATTACCTTCTTTCACATGAAATAATCTTGAATGATAAAACAGATTGCTTCCTTGATGTTGCTTTTCTTTAAGTAATCTATTATTCCATACAAATAAATACATTATGGTGTATATGGAGGAAAAGCTGCATCATTCTATTTATAATCTGCTCCTCTCTGTATTGTATATGTCAGAAGTATGCATAATTGTACACA
ACTCTTCCCAGCCAGAAAGGATGTGAGGAACCAAACTTTTGTCATCATAGTAGTGGATAGTAGCCCACTTAGGAATCAGGATTGGGATTAAAACATTGAACAGTGGAGTCAAGAATCACGTCAAAATTTTCCATCCTTGTTTGAAGTATGAGAATGTTCCTAAATTTTACTGCTGGGAATGTTTTGAAAACTTATTTTCA
GAACTCATAGTATTTTGAAACGAATGGCACATTTCCTTCTCTCCATCTTATACAAACCCCATACAATATCAC

>TRINITY_DN43211_c1_g1_i6 from sample A7 / Example unspliced isoform:

ATCAAAGGCTTCAAGATCTTCAAGTCCAAAACCTTTCCTTTCTTTTCTTATATGATAAACATTGTGGACGGATTATCGGGCAATTAACTAATATCTCTTCGCGTGTCCATGCACAGAATGAGAAAGAGTTACGAACAAGCAAACTTTCACTTTTGTCGGAGAAAATACTGGACTTAAGGCGTATTCGACATCGTTGCTTG
AAGCTGGAGCAAAGAGGGCCCGAATTGAGCTTGCAAATTTTGATGAAAGAAGACGAAATACGGAGTATTGAAGCCAAAAATGAGCAGCTGAACTCAAAGCTCAGGGAAGTAACTCGCGAGGTTTACGAGGCAGATGAAGAGAGAAACCAGAATCAGAAATATTATGAGCTTCAGAAACAGGAAATGCAAAAGTTCGTGCA
AGAGTTTGAACAGCGTTGGTCAATCTTAAGgtctgaggtggaaagtctggaaaaaattgtttgtcagCTCGAATCAGCAGTGAGAGAAGTGCACGATACCAACAGATGTGTCTCCAGAAATGATGGTGAGAGTGCTTTTCAGTTTGTTGGTAGAGCAAAACTCTTCACAACAGAAAGTGAGCTTACTAGCAGCTTGTCCT
CTAGCCACTCGTTGAGAAGCTTTTTAGAGGCACAACTGGAGAGTACTCTTATTTCTCAAGATGAAGAGAGGAAGAAACTTTTGAAAGTTATGCAAAATCAAATAATTGACAATGGGCTTTTGCAAAGAGAGATCCAGGACCTTGAAGAAAAAGTGTTTGAAGTGATGAGAAGGCGAGATGAAAGGAAAAAGTCAGTTCTA
TCTTATCTGTACTTTTACTATTCCAACTCTCTTTTATCATCACATATTACCTTCTTTCACATGAAATAATCTTGAATGATAAAACAGATTGCTTCCTTGATGTTGCTTTTCTTTAAGTAATCTATTATTCCATACAAATAAATACATTATGGTGTATATGGAGGAAAAGCTGCATCATTCTATTTATAATCTGCTCCTCT
CTGTATTGTATATGTCAGAAGTATGCATAATTGTACACAACTCTTCCCAGCCAGAAAGGATGTGAGGAACCAAACTTTTGTCATCATAGTAGTGGATAGTAGCCCACTTAGGAATCAGGATTGGGATTAAAACATTGAACAGTGGAGTCAAGAATCACGTCAAAATTTTCCATCCTTGTTTGAAGTATGAGAATGTTCCT
AAATTTTACTGCTGGGAATGTTTTGAAAACTTATTTTCAGAACTCATAGTATTTTGAAACGAATGGCACATTTCCTTCTCTCCATCTTATACAAACCCCATACAATATCAC

Intron 2 GTCCAAAACC ... CCATGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	12.77	41	5.8	6
A2	4.76	40	6.3	2
A3	16.67	35	3.5	7
A4	27.57	25.9	3	9.9
A5	25	3	1	1
A6	8.7	57	9.3	5.4
A7	6.75	146	25	10.6
A8	6.27	119.7	20.3	8
A9	11.06	185	27.5	23
A10	9.66	163	25.5	17.4

>TRINITY_DN92600_c0_g1_i1 from sample A4 / Example spliced isoform

GGGGCAAAGTAATATATATGGCTGATTGAAGTTTGTGTACTGTGGAAACGGAACTTTTTAATGTGGAAACCGGGGATCGAACAGCTAGGCATTCACCAAAACGAGATTCTATTTACAACATGCAACCTTCTCTTCACTATGGCTTTGCCAAAACGATTCGATTCTCGGTTGCTTCAAATGGAATGCACCAGAAATCAAAG
GCTTCAAGATCTTCAAAATGAGAAAGAGTTACGAACAAGCAAACTTTCACTTTTGTCGGAGAAAATACTGGACTTGAGGCGTATTCGACATCGTTGCTTGAAGCTGGAGCAAAGAGGGCCCGAATTGAGCTTGCAAATTTTGATGAAAGAAGACGAAATACGGAGTATTGAAGCCAAAAATGAGCAGCTGAACTCAAAGC
TCAGGTTCTTTAACCCAGACGGAGCACACTTTGTCTTGTTTTAGAACTTATGGCAAGAACGTGTTGGGAAAAACGGGGTGTATTGCAAATGTTCCTATGAATGTGCTTAAATTGTGTCAAAGAACTGAAAGCTTTACCATAAGCTTTAATTTTTGGAAATTCCAG

>TRINITY_DN92600_c0_g1_i2 from sample A4 / Example unspliced isoform:

GGGGCAAAGTAATATATATGGCTGATTGAAGTTTGTGTACTGTGGAAACGGAACTTTTTAATGTGGAAACCGGGGATCGAACAGCTAGGCATTCACCAAAACGAGATTCTATTTACAACATGCAACCTTCTCTTCACTATGGCTTTGCCAAAACGATTCGATTCTCGGTTGCTTCAAATGGAATGCACCAGAAATCAAAG
GCTTCAAGATCTTCAAgtccaaaacctttcctttcttttcttatatgataaacattgttgacggattatcgggcaattatctaatatctcttcgcgtgtccatgcacagAATGAGAAAGAGTTACGAACAAGCAAACTTTCACTTTTGTCGGAGAAAATACTGGACTTGAGGCGTATTCGACATCGTTGCTTGAAGCTGG
AGCAAAGAGGGCCCGAATTGAGCTTGCAAATTTTGATGAAAGAAGACGAAATACGGAGTATTGAAGCCAAAAATGAGCAGCTGAACTCAAAGCTCAGGGAAGTAACTCGCGAGGTTTACGAGGCAGATGAAGAGAGAAACCAGAATCAGAAATATTATGAGCTTCAGAAACAGGAAATGCAAAAGTTCGAGCAAGAGTTT
GAACAGCGTTGGTCAATCTTAAGGTCTGAGGTGGAAAGTCTGGAAAAAATTGTTTGTCGGG