NA ----> NA

Intron 1 GTTAGAAACT ... GTTGTTCGAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.95	107.6	27.4	14
A2	18.57	106	13.7	19
A3	27.47	98.9	32.3	15
A4	21.77	93.9	19.1	18
A5	32.55	47.3	17.4	18
A6	26.98	797	209.7	180
A7	25.38	780	206.6	151
A8	25.25	787.6	200.9	171
A9	25.4	1058	274.1	215
A10	27.49	721.4	229.1	152

>TRINITY_DN101336_c0_g1_i1 from sample A2 / Example spliced isoform

AAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTCACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTT
CTTGAGGTTAAGATATATTCTTGAGATTATTATTACAAGTGAGAAATTAATAGTTGTGCCCATACACGGAAGGGCAGCAGAGATGCCGTGGTTTTATCCACTATGCTTGGAAACGAGTCGCCGCCATTGCTGGCTTGCAGGCTGGCTCTATATCTCTCTCACACACAGGAAGAATCTCTCGTTTATGGCAAACAGATTCC
GGCAGATGATGAAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAATACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAAGCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCA
AGATTATGCAACTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTTGCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTGAACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGA
ATATATGGATAGGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCCTTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCATTTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGT

>TRINITY_DN101336_c0_g1_i2 from sample A2 / Example unspliced isoform:

AAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTCACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTT
CTTGAGGTTAAGATATATTCTTGAGATTATTATTACAAGTGAGAAATTAATAGTTGTGCCCATACACGGAAGGGCAGCAGAGATGCCGTGGTTTTATCCACTATGCTTGGAAACGAGTCGCCGCCATTGCTGGCTTGCAGGCTGGCTCTATATCTCTCTCACACACCGGAAGAATCTCTCGTTTgttagaaactgaatca
tttctttcctattgcattcgttaaattgtttttctggttgtttcttgatttacactgaatttgttgttcgagatgtttcttgatttagattgaatttgttgttcgagATGGCAAACAGATTCCGGCAGATGATGAAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAA
TACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAAGCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAACTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTT
GCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTGAACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATAGGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCC
TTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCATTTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGT

Intron 2 GTTAAGATAT ... TGGCAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	85.38	25	18	146
A2	81.46	33	13	145
A3	81.13	30	8	129
A4	75.53	46	16	142
A5	80.72	16	5	67
A6	81.78	246	85	1104
A7	77.61	330	127	1144
A8	76.82	343	128	1137
A9	78.24	430	157	1546
A10	78.22	277	122	995

>TRINITY_DN31968_c0_g1_i2 from sample A6 / Example spliced isoform

GGATGGTAAGTGTTTTGAAATATATATAAACTGAAACTGGTTGCGAACGAGTTACAAATTTGAAAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTC
ACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTTCTTGAGATTCCGGCAGATGATGAAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAATACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAA
GCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAACTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTTGCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTG
AACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATAGGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCCTTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCAT
TTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGTGGTAGACGGACTTTGTCCGCTGACCTGTTCAGTACAGATGGAATTTCTGAAATCGTAGCATCTGTCC

>TRINITY_DN31968_c0_g1_i3 from sample A6 / Example unspliced isoform:

GGATGGTAAGTGTTTTGAAATATATATAAACTGAAACTGGTTGCGAACGAGTTACAAATTTGAAAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTC
ACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTTCTTGAGgttaagatatattcttgagattattattacaagtgagaaattaatagttgtgcccatacacggaagggcagcagagatgccgtggttttatccactatgcttggaaacgagtcgccgccattgctggcttg
caggctggctctatatctctctcacacaccggaagaatctctcgtttatggcaaacagATTCCGGCAGATGATGAAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAATACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAAGCGCGCTGTCA
TTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAACTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTTGCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTGAACAAGGAGGC
TGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATAGGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCCTTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCATTTTCAGCATTT
AATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGTGGTAGACGGACTTTGTCCGCTGACCTGTTCAGTACAGATGGAATTTCTGAAATCGTAGCATCTGTCC

Intron 3 GTTAGAAACT ... TGGCAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	16.44	146.1	27.5	30
A2	15.2	143.6	13.5	38
A3	17.75	139	32	28
A4	19.77	142	19	51
A5	25.66	63	17.5	26
A6	18.34	1136.6	209.5	301
A7	19.41	1158	206.5	351
A8	19.71	1162	200.5	370
A9	18.87	1564.8	274	454
A10	20.45	1036.8	229	304

>TRINITY_DN101336_c0_g1_i3 from sample A2 / Example spliced isoform

AAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTCACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTT
CTTGAGGTTAAGATATATTCTTGAGATTATTATTACAAGTGAGAAATTAATAGTTGTGCCCATACACGGAAGGGCAGCAGAGATGCCGTGGTTTTATCCACTATGCTTGGAAACGAGTCGCCGCCATTGCTGGCTTGCAGGCTGGCTCTATATCTCTCTCACACACCGGAAGAATCTCTCGTTTATTCCGGCAGATGATG
AAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAATACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAAGCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAA
CTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTTGCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTGAACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATA
GGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCCTTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCATTTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGT

>TRINITY_DN101336_c0_g1_i2 from sample A2 / Example unspliced isoform:

AAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTCACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTT
CTTGAGGTTAAGATATATTCTTGAGATTATTATTACAAGTGAGAAATTAATAGTTGTGCCCATACACGGAAGGGCAGCAGAGATGCCGTGGTTTTATCCACTATGCTTGGAAACGAGTCGCCGCCATTGCTGGCTTGCAGGCTGGCTCTATATCTCTCTCACACACCGGAAGAATCTCTCGTTTgttagaaactgaatca
tttctttcctattgcattcgttaaattgtttttctggttgtttcttgatttacactgaatttgttgttcgagatgtttcttgatttagattgaatttgttgttcgagatggcaaacagATTCCGGCAGATGATGAAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAA
TACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAAGCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAACTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTT
GCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTGAACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATAGGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCC
TTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCATTTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGT

Intron 4 GTTAAGATAT ... TGGCAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	48.98	25	18	30
A2	43.59	33	13	38
A3	37.5	30	8	28
A4	42.14	46	16	51
A5	49.21	16	5	26
A6	43.96	246	85	301
A7	42	330	127	351
A8	42.06	343	128	370
A9	41.54	430	157	454
A10	43.47	277	122	304

>TRINITY_DN31968_c0_g1_i2 from sample A6 / Example spliced isoform

GGATGGTAAGTGTTTTGAAATATATATAAACTGAAACTGGTTGCGAACGAGTTACAAATTTGAAAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTC
ACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTTCTTGAGATTCCGGCAGATGATGAAGCAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAATACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAA
GCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAACTCTGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTTGCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTG
AACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATAGGATCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCCTTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCAT
TTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGTGGTAGACGGACTTTGTCCGCTGACCTGTTCAGTACAGATGGAATTTCTGAAATCGTAGCATCTGTCC

>TRINITY_DN31968_c0_g1_i1 from sample A6 / Example unspliced isoform:

GGATGGTAAGTGTTTTGAAATATATATAAACTGAAACTGGTTGCGAACGAGTTACAAATTTGAAAGGAAGTGAAAATATCTATGAATTTCCCATGTAGGACGGTAGAGTTCAGACGTAAATATCGAAAGCTGCGTGGTTGGATTCGATGGCGGCATTAACACGCCACCGTCACAACGGCAATGGAATGGCATCAATTTTC
ACCACTAATCCAACCCAACAATGCACGCAATGTCTTGTTACGTCTTAATCCTTCCAACAACTTCTTGAGgttaagatatattcttgagattattattacaagtgagaaattaatagttgtgcccatacacggaagggcagcagagatgccgtggttttatccactatgcttggaaacgagtcgccgccattgctggcttg
caggctggctctatatctctctcacacaccggaagaatctctcgtttgttagaaactgaatcatttctttcctattgcattcgttaaattgtttttctggttgtttcttgatttacactgaatttgttgttcgagatgtttcttgatttagattgaatttgttgttcgagatggcaaacagATTCCGGCAGATGATGAAG
CAAGATAGGGACGATTCGGACATTTCTGCAGCACCAGGAAGAAGAGAGTTGATTTCTCATGAATACATGAAGAAACGCTCTAATTCAAGTACTTTGAAGAAGAGGCTTTTAAGCGCGCTGTCATTTTATTTCTCAGGATGCCGGGCCGGCTCAAGTCTATCAGTGAAAGGGCCGGATGATCTTCCAAGATTATGCAACTC
TGATTTAAGTAGAAGGTATCACCATCAGCCTGATGAGACTGCATCAAGCTCCAGTACTAATTTGCCCTATAAAAGCAAGAATCCGAATCTGAATAATCTTGTGGAAGATGTGAACAAGGAGGCTGATAATTTCATAGCCAAAATGCGAGAGGGATTGAAGATCGAGAGGAAAGTGGCGGAGATGGAATATATGGATAGGA
TCCAAAGGGGTGAATGATATGATATGATCTTCTTCTTGTATATAATATATCTATATCTTGGCCTTTAAATTGGACGTTATATATTGTACTGCCATTGCACAGTGCTAACCATTTTCAGCATTTAATGCTTCTGTTCTGCTTATTCATGATATGCCAGGAAAAACTTGTGGTAGACGGACTTTGTCCGCTGACCTGTTCAG
TACAGATGGAATTTCTGAAATCGTAGCATCTGTCC