sp|B9DFG5|PTI13_ARATH ----> PTI1-like tyrosine-protein kinase 3

Intron 1 GTATGTATGG ... GTATTTGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	2	9
A2	81.82	1	2	7
A3	77.27	2.7	14	4
A4	81.82	2	8	10
A5	75.56	1.3	2	6
A6	-1	0	0	0
A7	72.41	4	13	8
A8	52.57	3	2	4.7
A9	33.33	4	0	4
A10	100	0	0	2

>TRINITY_DN45270_c0_g9_i1 from sample A7 / Example spliced isoform

ATTTTTCTTTTTCTACTGCTAGGTTTTTTTTTCCTGCTTTTTGTGAGCATGGCCATGCTAATTTGTGGTTTGGTATTTGTTTCCCTGTTTTCCTTTTCATCTGCTTTTATGTTTTTACTTTTTATAGTTTTTTTGGTTTCAATGGATGGGGAAAATATTGTGGGTTTAGGGCTGATGCAAATTCCACTAGTGTAGCAGTC
AAAGGCAGGGGAGAGGAATGCATGTAATGGAAGGTGAAGGAGCACTGATGAAACTATTGAATATCTTTGTGTGAGATAGAGGTGTGGTGCACTATAAAAGAGGCAGAGGCAAAGGGTTTTTGAAAGCTTCTGCAGTCCTCAATTTGTGTATTCCCCCCTTTCTTCCCGAGCAGGGTCCCTATACTTGCAAAATGCAGCCT
GCTTTGGAATGCTGCCAATAGGCATTTTGCAACTATGAATATGCTCATTCTTCAAATGAAGTAGCAACAATTCAACCATTCTCTAAAGAAGCTGGGTTTCCTTGCAGTCAGTGAAATTTCTCTAGTAGAGAAGAAACTTATCTGGAAAGGAGATTGGAGTGTCTCTGTTGAAGTAAAACCTGCAGGCATTTTATATATCA
GGAGTCACAATCTGCCACTTCTGGCTTTTCAACAGGTTGCATTTCTAGTTCTCTTCCTAGAGTTTTTCATTTATGTGCTTCTGCTTTGAATGGAAAATCTCCCTTTTAAGTGTTTATTCATCCTGCTAACCCACTTCAGTTGTGTGACATACTTGTTTTTGCAGTTAATTTTATAGGAAGAGGAGGGTGGAGTCAAGTGT
ACAGAGGATTTCTTTCTGATTGACAATGCGTGTCAGTGAAAATCTTGAATTCTTCACCCAAACCAGAGCAAGAGCTTCTTTCTGACGTGGAGGCAATGAGAGACCAGTCCCTTGGAGAGTGCGATTGGAGGTGGCTGAAGCATTAGATGACTTGCATGCTGGCTTTTCTCCCTCTGTCATTCATGGAAATGTGAAATCTT
CCAATATCTTTGATTCTGAAGAATTTGAACCAGAGTTATACATCCATTTACTGCAAAGAGCATCAAGACTAAATTTGACAGTTCAGTATTCCAAGAAATGATAGTGATGTAAAGTTGTAAATTGAAACTTGAAATTCTGTACTAATGAAATTTTTTGACATAATTACTCTGGCTTTAATTATAATAGTTGTCAGATTTTG
GGTTGGCAAGGTGAGGACCAACATCTTCATATATCGCTTGCAATGATGTTTTAGGAA

>TRINITY_DN45270_c0_g9_i3 from sample A7 / Example unspliced isoform:

ATTTTTCTTTTTCTACTGCTAGGTTTTTTTTTCCTGCTTTTTGTGAGCATGGCCATGCTAATTTGTGGTTTGGTATTTGTTTCCCTGTTTTCCTTTTCATCTGCTTTTATGTTTTTACTTTTTATAGTTTTTTTGGTTTCAATGGATGGGGAAAATATTGTGGGTTTAGGGCTGATGCAAATTCCACTAGTGTAGCAGTC
AAAGGCAGGGGAGAGgtatgtatggaggaatttacatgttttctgctacatgatttatggttccatggcatttaatttaactcatttgctaattcataattatatcacgtgtatttgaagGAATGCATGTAATGGAAGGTGAAGGAGCACTGATGAAACTATTGAATATCTTTGTGTGAGATAGAGGTGTGGTGCACTAT
AAAAGAGGCAGAGGCAAAGGGTGAGTTTTATTCTTGTTCAAGACCCATGTGTGTTTTGCTGTGAAATCCTCATTTAATTTTAATGGTTAATGAGTTTTGGCCTCTACATAAGATGTACTTTTGCATATGATTACTCATAAGACTTTTGTATTATGTTTCATACTTCTCTTACTATTTTTCAGTCTCTATTTAATATTTCT
AATTCTCTTTCAGTCTCTATTTAATATTTCTAATTCTCTTTCAGTCTCTATTTAATATTTC

Intron 2 GTGATTCTTA ... TATATTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	100	0	11	10
A2	100	0	2	1
A3	66.67	1	2	2
A4	77.78	2	7	7
A5	-1	0	0	0
A6	0	1	0	0
A7	68.42	3	7	6
A8	14.29	6	0	2
A9	0	4	0	0
A10	33.33	1	1	0

>TRINITY_DN45270_c0_g9_i1 from sample A7 / Example spliced isoform

ATTTTTCTTTTTCTACTGCTAGGTTTTTTTTTCCTGCTTTTTGTGAGCATGGCCATGCTAATTTGTGGTTTGGTATTTGTTTCCCTGTTTTCCTTTTCATCTGCTTTTATGTTTTTACTTTTTATAGTTTTTTTGGTTTCAATGGATGGGGAAAATATTGTGGGTTTAGGGCTGATGCAAATTCCACTAGTGTAGCAGTC
AAAGGCAGGGGAGAGGAATGCATGTAATGGAAGGTGAAGGAGCACTGATGAAACTATTGAATATCTTTGTGTGAGATAGAGGTGTGGTGCACTATAAAAGAGGCAGAGGCAAAGGGTTTTTGAAAGCTTCTGCAGTCCTCAATTTGTGTATTCCCCCCTTTCTTCCCGAGCAGGGTCCCTATACTTGCAAAATGCAGCCT
GCTTTGGAATGCTGCCAATAGGCATTTTGCAACTATGAATATGCTCATTCTTCAAATGAAGTAGCAACAATTCAACCATTCTCTAAAGAAGCTGGGTTTCCTTGCAGTCAGTGAAATTTCTCTAGTAGAGAAGAAACTTATCTGGAAAGGAGATTGGAGTGTCTCTGTTGAAGTAAAACCTGCAGGCATTTTATATATCA
GGAGTCACAATCTGCCACTTCTGGCTTTTCAACAGGTTGCATTTCTAGTTCTCTTCCTAGAGTTTTTCATTTATGTGCTTCTGCTTTGAATGGAAAATCTCCCTTTTAAGTGTTTATTCATCCTGCTAACCCACTTCAGTTGTGTGACATACTTGTTTTTGCAGTTAATTTTATAGGAAGAGGAGGGTGGAGTCAAGTGT
ACAGAGGATTTCTTTCTGATTGACAATGCGTGTCAGTGAAAATCTTGAATTCTTCACCCAAACCAGAGCAAGAGCTTCTTTCTGACGTGGAGGCAATGAGAGACCAGTCCCTTGGAGAGTGCGATTGGAGGTGGCTGAAGCATTAGATGACTTGCATGCTGGCTTTTCTCCCTCTGTCATTCATGGAAATGTGAAATCTT
CCAATATCTTTGATTCTGAAGAATTTGAACCAGAGTTATACATCCATTTACTGCAAAGAGCATCAAGACTAAATTTGACAGTTCAGTATTCCAAGAAATGATAGTGATGTAAAGTTGTAAATTGAAACTTGAAATTCTGTACTAATGAAATTTTTTGACATAATTACTCTGGCTTTAATTATAATAGTTGTCAGATTTTG
GGTTGGCAAGGTGAGGACCAACATCTTCATATATCGCTTGCAATGATGTTTTAGGAA

>TRINITY_DN45270_c0_g9_i2 from sample A7 / Example unspliced isoform:

ATTTTTCTTTTTCTACTGCTAGGTTTTTTTTTCCTGCTTTTTGTGAGCATGGCCATGCTAATTTGTGGTTTGGTATTTGTTTCCCTGTTTTCCTTTTCATCTGCTTTTATGTTTTTACTTTTTATAGTTTTTTTGGTTTCAATGGATGGGGAAAATATTGTGGGTTTAGGGCTGATGCAAATTCCACTAGTGTAGCAGTC
AAAGGCAGGGGAGAGGAATGCATGTAATGGAAGGTGAAGGAGCACTGATGAAACTATTGAATATCTTTGTGTGAGATAGAGGTGTGGTGCACTATAAAAGAGGCAGAGGCAAAGGGTTTTTGAAAGCTTCTGCAGTCCTCAATTTGTGTATTCCCCCCTTTCTTCCCGAGCAGGGTCCCTATACTTGCAAAATGCAGCCT
GCTTTGGAATGCTGCCAATAGGCATTTTGCAACTATGAATATGCTCATTCTTCAAATGAAGTAGCAACAATTCAACCATTCTCTAAAGAAGCTGGGTTTCCTTGCAGTCAGTGAAATTTCTCTAGTAGAGAAGAAACTTATCTGGAAAGGAGATTGGAGTGTCTCTGTTGAAGTAAAACCTGCAGGCATTTTATATATCA
GGAGTCACAATCTGCCACTTCTGGCTTTTCAACAGGTTGCATTTCTAGTTCTCTTCCTAGAGTTTTTCATTTATGTGCTTCTGCTTTGAATGGAAAATCTCCCTTTTAAGTGTTTATTCATCCTGCTAACCCACTTCAGTTGTGTGACATACTTGTTTTTGCAGTTAATTTTATAGGAAGAGGAGGGTGGAGTCAAGTGT
ACAGAGGATTTCTTTCTGATTGACAATGCGTGTCAGTGAAAATCTTGAATTCTTCACCCAAACCAGAGCAAGAGCTTCTTTCTGACGTGgtgattcttactctattgaaaacatgaaatcatgtagaaatatttattagtacatcttatgaatcatagtgccatcattgtatggcagggtctttcattctcttttctgag
atttggttatagccgtagttattgactatatatattatatatttcagGAGGCAATGAGAGACCAGTCCCTTGGAGAGTGCGATTGGAGGTGGCTGAAGCATTAGATGACTTGCATGCTGGCTTTTCTCCCTCTGTCATTCATGGAAATGTGAAATCTTCCAATATCTTTGATTCTGAAGAATTTGAACCAGAGTTATACA
TCCATTTACTGCAAAGAGCATCAAGACTAAATTTGACAGTTCAGTATTCCAAGAAATGATAGTGATGTAAAGTTGTAAATTGAAACTTGAAATTCTGTACTAATGAAATTTTTTGACATAATTACTCTGGCTTTAATTATAATAGTTGTCAGATTTTGGGTTGGCAAGGTGAGGACCAACATCTTCATATATCGCTTGCA
ATGATGTTTTAGGAA