NA ----> NA

Intron 1 GTGGATTTCT ... TCGAGTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	52.02	13	15	13.7
A2	59.56	9	12	14.7
A3	64.9	9	19	14.3
A4	72.35	6	20	11.7
A5	75.33	3	12	6.3
A6	49.43	30	32	27
A7	40.39	69	49	45
A8	47.31	59	56	51.3
A9	53.44	31	35	36.3
A10	34.55	60	39	24.7

>TRINITY_DN47138_c2_g1_i7 from sample A10 / Example spliced isoform

CCCATCCATAAGAGGATTTTAAAGATTAATAAACATAAAAAGGGAAATTTGAATTCTTTGACAGAAAATTTAGGAGGAGCGACATTTGTTAGCATCGTTGGGAGAAAGCAAAGATAGAGAGATAGAAGGAGAAGCCGAGCAGCAGATTTTAGGGTTTGTAAGATGCAATGTTTGGCAGATTTTAGGGTCTTCAGTTTTGG
GAGAAGGAAGTTCAAGAAGATCCACATAAGTCAGCAGATATTAGGGTCTGCTTAGCTCCGATAGAAGAAACGATGGAGAGATAAAGGGTGGAGCCGTGCAGTAAATCTTAGGGTTTGTTGGCTTGGCCGGAGTAAATTTTAATGCGTTGGGAGTAGAAAGTTGAAGAAGACCCACATGTGTTGGCTTGGCCGGAGCCAAA
CCAAGAAAATATCGCGGAGTAACACTGTTTGTATTTCTTTCTTTTGCTTCGACCATGGCAACGCTTACTTAATCTCACTTTTCATTCTGTTTGTTGGGATCGAAAAGGATGATGAGAAATTAAAAATATCCAGAAGTGGGCATCACTATGTGGCTAATTGGGAAG

>TRINITY_DN47138_c2_g1_i2 from sample A10 / Example unspliced isoform:

CCCATCCATAAGAGGATTTTAAAGATTAATAAACATAAAAAGGGAAATTTGAATTCTTTGACAGAAAATTTAGGAGGAGCGACATTTGTTAGCATCGTTGGGAGAAAGCAAAGATAGAGAGATAGAAGGAGAAGCCGAGCAGCAGATTTTAGGGTTTGTAAGATGCAATGTTTGGCAGATTTTAGGGTCTTCAGTTTTGG
GAGAAGGAAGTTCAAGAAGATCCACATAAGTCAGCAGATATTAGGGTCTGCTTAGCTCCGATAGAAGAAACGATGGAGAGATAAAGGGTGGAGCCGTGCAGTAAATCTTAGGGTTTGTTGGCTTGGCCGGAGTAAATTTTAATGCGTTGGGAGTAGAAAGTTGAAGAAGACCCACATGTGTTGGCTTGGCCGGAGCCAAA
CCAAGAAAATATCGCGGAGTAACACTGTTTGTATTTCTTTCTTTTGCTTCGACCATGGCAACGCTTACTTAATCTCACTTTTCATTCTGgtggatttcttttttccatttttctttttcttttaaatctttattttcttaagttttgctttgttcagctctgttttttttctttctaactatttcatttttgtttgcttg
ttcattctgtttcgagtctagTTTGTTGGGATCGAAAAGGATGATGAGAAATTAAAAATATCCAGAAGTGGGCATCACTATGTGGCTAATTGGGAAGGTGTAGAAGTCTTATACTCTTAATAAATATTTAAAATTTTGAATGGGAATAATCCATTACATCATAATTGCATCATTTCATCATGTTCTGTCTTGTCCCCAAA
TGCAGAGCTGTAGCTATGGGAGCAATAATTAGGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAG

Intron 2 GTTTGTATTT ... TCGAGTCTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.49	10.4	40.6	12.4
A2	67.7	16.4	32.3	13.9
A3	74.9	10	29.9	13.4
A4	70.12	15	35.3	10.4
A5	73.45	6.1	16.9	5.4
A6	61.12	49.1	76.4	24.3
A7	66.31	66	130	36.9
A8	55.19	86.1	105.7	45
A9	55.67	78.6	98.1	30.3
A10	61.5	67.9	106.4	19.3

>TRINITY_DN120550_c0_g2_i1 from sample A3 / Example spliced isoform

AGCAGTAAATCTTAGGGTTTGTAAGATGCAGTAAATTTTAATGCGTTGGGAGTAGAAAGTTGGGAGCAGTAAATCTTAGGGTGGAGCCGAGCAGTAAATTTTAATGCGTTGGGAGTAGAAAGTTGAAGAATACCCACATCTGTTGGCTTGGCCGGAGACAAACCAAGAAAATATCGCAGAGTAACGCTTTTGTTGGGATC
GAAAAGGATGATGAGAAATTAAAAATATCCAGAGAGCCATAGCTATGGGAGCAATAATTAGGTGCAGAAATCACAAATGGGAGCAATAATTAGGTGCAAAAATCACAGATGCTGCGGAATTTCGGGATTGCGGGTGGGTTTTTAGGTTCAAAGGCTTATATATGTGTTTGCAGTTTTTATTTACCCT

>TRINITY_DN120550_c0_g2_i7 from sample A3 / Example unspliced isoform:

GTGGGAAGAAAGCAAAGATAGAGAGATAGAAGGAGAAGCCGAGCAGCAGATTTTAGGGTTTGTAAGATGCAATGTTTGGCAGATTTTAGGGTCTTCAGTTTTGGGAGAAGGAAGTTCAAGAAGATCCACATAAGTCAGCAGATATTAGGGTCTTCTTAGCTCCGGTAGAAGAAACAATGGAGAGATAAAGGGTGGAGCCG
AGCAGTAAATCTTAGGGTTTGTAAGATGCAGTAAATTTTAATGCGTTGGGAGTAGAAAGTTGGGAGCAGTAAATCTTAGGGTGGAGCCGAGCAGTAAATTTTAATGCGTTGGGAGTAGAAAGTTGAAGAAGACCCACATCTGTTGGCTTGGCCGGAGACAAACCAAGAAAATATCGCAGAGTAACGCTgtttgtatttct
ttcttttgcttcaaccatggcaacgcttacttaatctcacttttcattctggtggatttcttttttccatttttctttttcttttaaatctttattttcttaagttttgctttgttcagctctgttttttttctttctaactatttcatttttgtttgcttgttcattctgtttcgagtctagTTTGTTGGGATCGAAAA
GGATGATGAGAAATTAAAAATATCCAGAGAGCCATAGCTATGGGAGCAATAATTAGGTGCAGAAATCACAAATGGGAGCAATAATTAGGTGCAAAAATCACAGATGCTGCGGAATTTCGGGATTGCGGGTGGGTTTTTAGGTTCAAAGGCTTATATATGTGTTTGCAGTTTTTATTTACCCT

Intron 3 GTGTAGAAGT ... CCAAATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.75	13.6	30	19
A2	56.31	8.6	10	12
A3	76.95	5	24	9
A4	78.8	5.8	21	17
A5	58.77	4	8	3
A6	35.6	32.6	22	14
A7	30.92	53.8	24	24
A8	22.16	63.4	19	17
A9	48.31	34.4	32	32
A10	26.9	33.2	19	5

>TRINITY_DN45917_c0_g1_i4 from sample A7 / Example spliced isoform

GTTCAGCTCTGTTTTTTTTCTTTCTAACTATTTCATTTTTGTTTGCTTGTTCATTCTGTTTCGAGTCTAGTTTGTTGGGATCGAAAAGGATGATGAGAAATTAAAAATATCCAGAAGTGGGCATCACTATGTGGCTAATTGGGAAGAGCTGTAGCTATGGGAGCAATAATTAGGTGCAGAAATCACAGATGGGAGCAATA
ATTAGGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAG

>TRINITY_DN45917_c0_g1_i9 from sample A7 / Example unspliced isoform:

GTTCAGCTCTGTTTTTTTTCTTTCTAACTATTTCATTTTTGTTTGCTTGTTCATTCTGTTTCGAGTCTAGTTTGTTGGGATCGAAAAGGATGATGAGAAATTAAAAATATCCAGAAGTGGGCATCACTATGTGGCTAATTGGGAAGgtgtagaagtcttatactcttaataaatatttaaaattttgaatgggaataatc
cattacatcataattgcatcatttcatcatgttctgtcttgtccccaaatgcagAGCTGTAGCTATGGGAGCAATAATTAGGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAG

Intron 4 GTGCAGAAAT ... CAATAATTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	66.43	10.3	18	5.5
A2	65.68	5.8	9.5	1.3
A3	68.75	2.5	4.7	3.2
A4	76.92	2.7	9.8	3.3
A5	57.78	2.2	2.8	1.3
A6	34.95	23.5	13.2	7
A7	30.01	45	21.8	12.2
A8	27.96	53.8	23	14.2
A9	48.03	25.2	23.3	10.8
A10	18.09	28.8	8.2	4.7

>TRINITY_DN45917_c0_g1_i4 from sample A7 / Example spliced isoform

GTTCAGCTCTGTTTTTTTTCTTTCTAACTATTTCATTTTTGTTTGCTTGTTCATTCTGTTTCGAGTCTAGTTTGTTGGGATCGAAAAGGATGATGAGAAATTAAAAATATCCAGAAGTGGGCATCACTATGTGGCTAATTGGGAAGAGCTGTAGCTATGGGAGCAATAATTAGGTGCAGAAATCACAGATGGGAGCAATA
ATTAGGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAG

>TRINITY_DN45917_c0_g1_i9 from sample A7 / Example unspliced isoform:

GTTCAGCTCTGTTTTTTTTCTTTCTAACTATTTCATTTTTGTTTGCTTGTTCATTCTGTTTCGAGTCTAGTTTGTTGGGATCGAAAAGGATGATGAGAAATTAAAAATATCCAGAAGTGGGCATCACTATGTGGCTAATTGGGAAGGTGTAGAAGTCTTATACTCTTAATAAATATTTAAAATTTTGAATGGGAATAATC
CATTACATCATAATTGCATCATTTCATCATGTTCTGTCTTGTCCCCAAATGCAGAGCTGTAGCTATGGGAGCAATAATTAGgtgcagaaatcacagatgggagcaataattagGTGCAGAAATCACAGATGGGAGCAATAATTAGGTGCAG