sp|Q9SGE0|AXS2_ARATH ----> UDP-D-apiose/UDP-D-xylose synthase 2

Intron 1 GTAAGTGTAA ... CATCTCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20	29.5	8	7
A2	10	33	3	4.5
A3	6.51	43	4	2
A4	11.99	40	5.5	5.5
A5	18.26	24	6	5
A6	15.58	187.5	37.5	32.5
A7	13.82	171.5	27	29
A8	16.41	228	43	48.5
A9	12.51	186	25.5	29
A10	19.96	158	48	31.5

>TRINITY_DN48568_c2_g1_i9 from sample A10 / Example spliced isoform

ATCCACTCATACTTGAAAAGGGAGCCGTGACCTGCGAGGGGTCGGGCGAGTTTTCAATGCGGAGAGAAGAAGAATACTGGTACAGTTGCTGGTAGTTGGACTCGAACCCTGGACCTGTAACGTGGCCTACAACCCTAGGTTTTTTGGGGGAAAATGGCATGCATGGACGAAAACGAGCTCATACATAAGCTCTTTGAATC
CGGGAAGCGGCTCGAACACCCGCCTCAGTCTAAGGATAATCTTGTTTAGGCTTTAGAGGAAATTCCTTCCAGGGAAAACAGTCACATTTTCAATGTTGGCAATCCTAATAATGAGGTCACTGTCAAGCAACTGACAGAAATAATCACTGAGTTTGCAATAAATTTGCTCCTCAGTTAACATTATAACGAACTTGGAGGAA
CAAACAGGAGGATGAGCTGGGTGACATGATTCCAAAGAAGATCCATATGTCTGATTTCCATTTGTGCATCTGCCATTGGTCATCACCATCTACTAGGGTTACATTCTGCTTGTGACCTGCTCCACTTGATTGACATGAGTTTAAAAGGGATGAGGCCAGGTACTAGTCCCAAGCAATTTTGATGGGTATAGCTACACCTC
ATATTGGATAGCTAAGCAAAGAAAGTCTACCCTATGTAACCCATCATAGGAAGTTTTTTGAAGCATTTCCAAAAAAAAGTGCAATTTCATGCTAATATGAATCTTAGGAATTTTGCACTTTTATAGGTGCTCTACATTTATGTTATATATGTGAATTAAAAAATGCATGTAAGTTGAGATAACACTGATAATTCACAAGA
TATGTTACTTTGTTGTTTATTCAAGCACGTTTATATACCTGCTGAAACTAATGCTTGGATTCAGGAACATGACAATTATAAAGAAATTTTTTTCAAGTAGTACAATGAGAAAAAAATACAGATTAAAGTTAGAGGCTCTATCTTTTGCATAGCCTCCTTTACTAAAGCATTTAATTTATTATATATCTTGGTCTCCACGG

>TRINITY_DN48568_c2_g1_i2 from sample A10 / Example unspliced isoform:

ATCCACTCATACTTGAAAAGGGAGCCGTGACCTGCGAGGGGTCGGGCGAGTTTTCAATGCGGAGAGAAGAAGAATACTGGTACAGTTGCTGGTAGTTGGACTCGAACCCTGGACCTGTAACGTGGCCTACAACCCTAGGTTTTTTGGGGGAAAATGGCATGCATGGACGAAAACGAGCTCATACATAAGCTCTTTGAATC
CGGGAAGCGGCTCGAACACCCGCCTCAGTCTAAGGATAATCTTGTTTAGGCTTTAGAGGAAATTCCTTCCAGGGAAAACAGTCACATTTTCAATGTTGGCAATCCTAATAATGAGGTCACTGTCAAGCAACTGACAGAAATAATCACTGAGTTTGCAATAAATTTGCTCCTCAGTTAACATTATAACGAACTTGGAGGAA
CAAACAGGAGGATGAGCTGGGTGACATGATTCCAAAGAAGATCCATATGTCTGATTTCCATTTGTGCATCTgtaagtgtaaaaaacaactcatctcacagGCCATTGGTCATCACCATCTACTAGGGTTACATTCTGCTTGTGACCTGCTCCACTTGATTGACATGAGTTTAAAAGGGATGAGGCCAGGTACTAGTCCCA
AGCAATTTTGATGGGTATAGCTACACCTCATATTGGATAGCTAAGCAAAGAAAGTCTACCCTATGTAACCCATCATAGGAAGTTTTTTGAAGCATTTCCAAAAAAAAGTGCAATTTCATGCTAATATGAATCTTAGGAATTTTGCACTTTTATAGGTGCTCTACATTTATGTTATATATGTGAATTAAAAAATGCATGTA
AGTTGAGATAACACTGATAATTCACAAGATATGTTACTTTGTTGTTTATTCAAGCACGTTTATATACCTGCTGAAACTAATGCTTGGATTCAGGAACATGACAATTATAAAGAAATTTTTTTCAAGTAGTACAATGAGAAAAAAATACAGATTAAAGTTAGAGGCTCTATCTTTTGCATAGCCTCCTTTACTAAAGCATT
TAATTTATTATATATCTTGGTCTCCACGG

Intron 2 GTAGTTGAGA ... TGGATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	40.16	6	3	5
A2	33.33	7	1	6
A3	63.64	2	1	6
A4	33.33	4	2	2
A5	54.55	5	0	12
A6	51.78	8.5	10	8
A7	45.79	7.5	5.5	7
A8	54.59	14	17.5	15
A9	49.67	15	14.5	13
A10	55.55	8.5	10.5	10

>TRINITY_DN42405_c1_g2_i4 from sample A6 / Example spliced isoform

ATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGGAAGATCGGGGCTGAACAAGTCATGAAGAAAGGGATGTTCGTTGCTTGTAGAGGGATCCTTGGGGCTCAAATCATCAATCAAAGCACAGCT
GTGACAGGGCAGCTGTAACAGAATTTGCTATCTTAAGTATGTTGCTGCTCGAGTCTAGCTGTGAGCTGGAAGGACCGCCACTCTTGGGTCAATTAATAACTTTCTCATATTTGGATGTTTCTGTTCTGCCACGCATTGTAACAGCTAGTTATTTAGGGCGCCTGAAGATCAGAGATCTTCATGGCGGCCCAATTATAGGA
TTAGATGTTACGATTTGAATGTATAAATATGGGGAGTTGCCTCGACCCAGGGGTTGAACAGCAGACAATTAATTTATTGTATTAGCAGCTGAGCAAAACTGTGTACTCATTCTTCTGTTAATATAAGGGGTTACTTCTGAGCTAA

>TRINITY_DN42405_c1_g2_i6 from sample A6 / Example unspliced isoform:

ATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGgtagttgagacaaatggattgcagGAAGATCGGGGCTGAACAAGTCATGAAGAAAGGGATGTTCGTTGCTTGTAGAGGGATCCTTGGGGCT
CAAATCATCAATCAAAGCACAGCTGTGACAGGGCAGCTGTAACAGAATTTGCTATCTTAAGTATGTTGCTGCTCGAGTCTAGCTGTGAGCTGGAAGGACCGCCACTCTTGGGTCAATTAATAACTTTCTCATATTTGGATGTTTCTGTTCTGCCACGCATTGTAACAGCTAGTTATTTAGGGCGCCTGAAGATCAGAGAT
CTTCATGGCGGCCCAATTATAGGATTAGATGTTACGATTTGAATGTATAAATATGGGGAGTTGCCTCGACCCAGGGGTTGAACAGCAGACAATTAATTTATTGTATTAGCAGCTGAGCAAAACTGTGTACTCATTCTTCTGTTAATATAAGGGGTTACTTCTGAGCTAA

Intron 3 GTGCGTCTCT ... GTTTATTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	67.14	4	6	10
A2	72.91	2	4.5	5.5
A3	100	0	3	4
A4	68.42	3	7	6
A5	66.67	1	0	4
A6	33.33	5.5	4.5	1
A7	39.77	14	5.5	13
A8	49.37	8.5	9.5	6
A9	23.91	26	11	6
A10	35.92	12	5.5	8

>TRINITY_DN46972_c0_g1_i2 from sample A7 / Example spliced isoform

ATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGTATCAACATGATTTCCTCCAATCCAATCCAATGTAGTTTGGTTGGTTAAATATATTGTATAAAGGCGTAGCATCCATCCATTAGTCTATTG
GGTCTAGTTATTCTCCTAGATATGTTATATAAAAAACATCAATCCATGCACTTGTTTCTTTTCAGTTGCTGGAACAAGTACTCTTCTCAATTCTGGATTCACTTAAATATAAAATAATTTTTCA

>TRINITY_DN46972_c0_g1_i1 from sample A7 / Example unspliced isoform:

ATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGgtgcgtctctgtatctctctttaattaatggttatcttagtgttttttttcatattcatcattcatgaatttttcctctttattgatcatg
agcattgttttcttgtgtttattcagTATCAACATGATTTCCTCCAATCCAATCCAATGTAGTTTGGTTGGTTAAATATATTGTATAAAGGCGTAGCATCCATCCATTAGTCTATTGGGTCTAGTTATTCTCCTAGATATGTTATATAAAAAACATCAATCCATGCACTTGTTTCTTTTCAGTTGCTGGAACAAGTACTC
TTCTCAATTCTGGATTCACTTAAATATAAAATAATTTTTCA

Intron 4 GTGTCATTAT ... CTTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.83	80	65	24
A2	44	56	44	22
A3	43.66	40	31	15
A4	43.21	46	35	32
A5	56.36	24	31	9
A6	41.6	372	265	30
A7	45.74	605	510	63
A8	44.05	475	374	73
A9	43.79	733	571	111
A10	44.97	711	581	79

>TRINITY_DN100926_c0_g1_i10 from sample A4 / Example spliced isoform

AATGCTCATCTGATCCTCTAACGGTCAAGTAGGGATGGAATTTATCTCTCTCTATATATACACTGTTTAGACGTTTTTGCTTACATCCTCCTCCACTGTCATTTGTAGATTCTCTCTGTTGTTTTTCATTTGTATTGGAAGCTCTTTCTGTGTGTTTCTGTCTGGGTGTGTGTTTTGTATATGTGACATTGTGTGTTTGT
GCATGTTACTGTGTATGTGTCATGTAGTTTATGATAAAGGAGGAGGATATATATTCTTTCGATTTTAATTTGGCAGGCAGACGTTATTGCTCGATGCCAGGGAGATCGGTCTTTGCTTGGATTCATTGAATGGCTTCTTGATTGAATCGTCTCATGGAGGTATCACTCTGCTCTTCTCTGTTTTCATCGGATGGCCTTCG
ACCTGATATATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGGTGCGTCTCTGTATCTCTCTTTAATTAATGGTTATCTTAGTGTTTTTTTTCATATTCATCATTCATGAATTTTTCCTCTTTA
TTGATCATGAGCATTGTTTTCTTGTGTTTATTCAGTATCAACATGATTTCCTCCAATCCAATCCAATGTAGTTTGGTTGGTTAAATATATTGTATAAAGGCGTAGCATCCATCCATTAGTCTATTGGGTCTAGTTATTCTCCTAGATATGTTATATAAAAAACATCAATCCATGCACTTGTTTCTTTTCAGTTGCTGGAA
CAAGTACTCTTCTCAATTCTGGATTCACTTAAATATAAAATAATTTTCGAAATATGACTTTTTGTGTCTTAATATGATTCTC

>TRINITY_DN100926_c0_g1_i3 from sample A4 / Example unspliced isoform:

AATGCTCATCTGATCCTCTAACGGTCAAGTAGGGATGGAATTTATCTCTCTCTATATATACACTGTTTAGACGTTTTTGCTTACATCCTCCTCCACTGTCATTTGTAGATTCTCTCTGTTGTTTTTCATTTGTATTGGAAGCTCTTTCTGTGTGTTTCTGTCTGGGTGTGTGTTTTGTATATGTGACATTGTGTGTTTGT
GCATGTTACTGTGTCTGTGTCATGTAGTTTATGATAAAGGAGGAGGATATATGTAATTTGGCGGGCAGATATCATTGCTGGATGCCAGGGAGATCGGTCTTTGCTTGGATTCATTGAATGGCTTCTTGATTGAATCGTCTCATGGAGgtgtcattatgctctgctcggttttcatgcttcggcctggtatatttatagca
ttgtaatctgtcttcttgattgaatagactcatggatgcttcttgcagGTATCACTCTGCTCTTCTCTGTTTTCATCGGATGGCCTTCGACCTGATATATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCC
ATGACAGGTGCGTCTCTGTATCTCTCTTTAATTAATGGTTATCTTAGTGTTTTTTTTCATATTCATCATTCATGAATTTTTCCTCTTTATTGATCATGAGCATTGTTTTCTTGTGTTTATTCAGTATCAACATGATTTCCTCCAATCCAATCCAATGTAGTTTGGTTGGTTAAATATATTGTATAAAGGCGTAGCATCCA
TCCATTAGTCTATTGGGTCTAGTTATTCTCCTAGATATGTTATATAAAAAACATCAATCCATGCACTTGTTTCTTTTCAGTTGCTGGAACAAGTACTCTTCTCAATTCTGGATTCACTTAAATATAAAATAATTTTCGAAATATGACTTTTTGTGTCTTAATATGATTCTC

Intron 5 GCAGGCCATT ... TATTGGGAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	53.85	3	6	1
A2	73.33	2	6	5
A3	100	0	2	2
A4	71.43	2	7	3
A5	100	0	2	4
A6	84.91	4	20	25
A7	84.31	4	21	22
A8	78.69	13	57	39
A9	73.91	15	46	39
A10	80.65	6	20	30

>TRINITY_DN42290_c3_g2_i7 from sample A8 / Example spliced isoform

GGTATCACTCTGCTCTTCTCTGTTTTCATCGGATGGCCTTCGACCTGATATATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGATCCATTTCATATCAACAAATAAAGGACAAACCAAGGTAG
TGATTGGTGTTCAGGTAACTATTATGGTGCTTGGTTGGAGAGAATATTGGTGATGGGATATGGTCATGTCACATGCTAATTTGTGTACAATATTTAAGTGAATCTATCTAATAATGGGAAATATTCCATTCCTAATAGTGTATGGATGATAGCCAAATAGAGTAAGAAGTTTGGTGATTTTTCCAAGAGCAGACTTGAAG
AGTGTGGATGAGGAGACATTTTCATAGTTCACGAGATATGTGCATGGATGGGTGAAGTAACGCAATTCTCAAGCTTGAATCACCTTGATTTTTCAAAACCATTTATAGTATTGATGTTCACAAGTCTCATTTGATATATTACAGTGCACG

>TRINITY_DN42290_c3_g2_i11 from sample A8 / Example unspliced isoform:

GGTATCACTCTGCTCTTCTCTGTTTTCATCGGATGGCCTTCGACCTGATATATCATTTATCCCTTGTGGAGAAAGTTGCGGTGGAGTGCGATCAAGAGATGAATCCCAGCCTAGGTCAAATGCAGATGATCTTAAACAATAAACGGCAGGCCCATGACAGgcaggccattggtcatcaccatctactagggttacattct
gcttgtgacctgctccacttgaatgacatgggtttaaaagggatgaggccaagtactagtcccaagcaattttgatgggtatagctacacctcgtattgggaagATCCATTTCATATCAACAAATAAAGGACAAACCAAGGTAGTGATTGGTGTTCAGGTAACTATTATGGTGCTTGGTTGGAGAGAATATTGGTGATGG
GATATGGTCATGTCACATGCTAATTTGTGTACAATATTTAAGTGAATCTATCTAATAATGGGAAATATTCCATTCCTAATAGTGTATGGATGATAGCCAAATAGAGTAAGAAGTTTGGTGATTTTTCCAAGAGCAGACTTGAAGAGTGTGGATGAGGAGACATTTTCATAGTTCACGAGATATGTGCATGGATGGGTGAA
GTAACGCAATTCTCAAGCTTGAATCACCTTGATTTTTCAAAACCATTTATAGTATTGATGTTCACAAGTCTCATTTGATATATTACAGTGCACG