sp|Q5W794|DI192_ORYSJ ----> Protein DEHYDRATION-INDUCED 19 homolog 2

Intron 1 GTATGTATGT ... CGGCGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	20.54	89	15.5	23
A2	12.04	95	16.5	13
A3	13.38	123	24.5	19
A4	11.46	85	14	11
A5	12.73	48	5	7
A6	12.95	625	103.5	93
A7	12.82	714	112	105
A8	15.2	597	88.5	107
A9	12.87	731	136.5	108
A10	12.38	644	112	91

>TRINITY_DN113277_c0_g1_i2 from sample A1 / Example spliced isoform

ATTGGTTCATATCTTCTTCCTTATAAATCGAATATCTCTCATTTCCATTCAATCTCCTTTCTTTTTCTCTATCCATATAGCATAATCCCTTCTAAACTAAACTAATGGTGACGGACTTGTAATTCTGACTTGCTTTCCTTTCATTTTGCAATTTCCCCTTTGCTATTCTTGGGAGTCGGGACAAGAATTATCAGCCATCC
TCTTCGTCCTTGTGGTGATTACTTGATATGGCTTCCGGGAGAAAATTATTGGCAGAAGATGTGCCATGGAGGGCGACTGCGAAAGGGAGGCCCGTTCCCAGAATATCTCAAGCGCCTCTCCTCGCTGTTCGCCACTCTCCCAATTCAAATTACGCCATCAGCATCATGAAGCATCCGGACCCCATTGGGAAGGGACTGGC
AAGTGAAGCTTTCGTGGAGTCGGCAGGGCCTGATTGCATCGTTCCAGGCCAAATGAGGCCCATCAAGCTGCTTGGATTACAGCTGTGGCCGATTGAGATCAATTGGAAATTTATGAAACCGGTTGCACGAGAGCTGAAAACAGTAGGCAAGCTTGTTGATTCAGCGTTTGATCTGATGCAAGCCCCTTTTGGTGACCGAT
AGTTGCCTATTCAACTTTGATGTCTACTTTGTATTGGCAAGGCTCGAGCGAGTAGTCAGGACAAGCCTGAGACAAGTTGTCCCTCAAGTAAATACTTTCTATTCAAGTCCAACTTGAGTTGGTCCAGTCCCA

>TRINITY_DN113277_c0_g1_i4 from sample A1 / Example unspliced isoform:

ATTGGTTCATATCTTCTTCCTTATAAATCGAATATCTCTCATTTCCATTCAATCTCCTTTCTTTTTCTCTATCCATATAGCATAATCCCTTCTAAACTAAACTAATGGTGACGGACTTGTAATTCTGACTTGCTTTCCTTTCATTTTGCAATTTCCCCTTTGCTATTCTTGGGAGTCGGGACAAGAATTATCAGCCATCC
TCTTCGTCCTTGTGGTGATTACTTGATATGGCTTCCGGGAGAAAATTATTGGCAGAAGATGTGCCATGGAGGGCGACTGCGAAAGGGAGGCCCGTTCCCAGAATATCTCAAGCGCCTCTCCTCGCTGTTCGCCACTCTCCCAATTCAAATTACGCCATCAGCATCATGAAGgtatgtatgtatctcaaatttaacagtca
cacggatgaggagcagcaggaggagcctgtttcttattcacgttggtggcattgaattgaatagaataataatgagataggattggatttggcggcgtgcagCATCCGGACCCCATTGGGAAGGGACTGGCAAGTGAAGCTTTCGTGGAGTCGGCAGGGCCTGATTGCATCGTTCCAGGCCAAATGAGGCCCATCAAGCT
GCTTGGATTACAGCTGTGGCCGATTGAGATCAATTGGAAATTTATGAAACCGGTTGCACGAGAGCTGAAAACAGTAGGCAAGCTTGTTGATTCAGCGTTTGATCTGATGCAAGCCCCTTTTGGTGACCGATAGTTGCCTATTCAACTTTGATGTCTACTTTGTATTGGCAAGGCTCGAGCGAGGTAGTGATTTTAAGCCT
AGGCATCCTCTTCACCATGTAACATGTGCATTATATATGAGATATTTATTGTAGTTTTCACAAGCTATAAGTCATCTTTATCTATACTGCCATGGAATAATTTCTTAGAAAAGAGGTGATTACAAGAGGAATGTAAACGTGATTTGAGTTTGTGCAATGTTGTCAAAAATTGAATGATAACTTGATAAGAAATGAAAAGT
TTTCGGTTTGCCAAAAAAATCAGTATAGAAGGAGCATATGATTTAAATACACAGGTCTAAAGATATGCTTTCAATATGTGCAGAACACATCACCTCACCATTTTCTAATGTTTCTAAATAGAAAGCCACATGATAAAAGGGAACATACTGGTTCTCCAAATCTTCATTCGCACTTGCATAGCTACAAGCACCTAACTCAA
CTCATGGCAGGAAATAACAAATAATAAAAGTTCAATCTCTTGCAAATACAGATGACACTACAGAGCTATAGTGTATTTTTTATCAGTAGTTCGATATATTCCCATTTTTTTTATGGGAAGGTTGTTTCTTATTGCCAAGCATATATAAAGAGTTATGGAAACTGTTTTGAGCTATAATTAAGAGCAAAGGGGTGCAGGTC
TACATGAATTCAATGGAAACCCCATCTCTTTCTGTGCAATCTATTGCTCTTTGGGAATTTATCTGTGCACCACACACTGCAGTGGGCCTCAGACTTTTTTTAAAATAAAA

Intron 2 GTAGTGATTT ... ATATGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	64.86	26	48	9
A2	63.16	35	60	5
A3	73.96	25	71	8
A4	65.45	19	36	6
A5	52.17	11	12	0
A6	68.48	198.4	431	36
A7	70.05	174	407	32
A8	67.22	282.4	579	83
A9	68.43	245	531	93
A10	64.59	159	290	60

>TRINITY_DN37376_c0_g1_i3 from sample A6 / Example spliced isoform

ATTACGCCATCAGCATCATGAAGGTATGTATGTATCTCAAATTTAACAGTCACACGGATGAGGAGCAGCAGGAGGAGCCTGTTTCTTATTCACGTTGGTGGCATTGAATTGAATAGAATAATAATGAGATAGGATTGGATTTGGCGGCGTGCAGCATCCGGACCCCATTGGGAAGGGACTGGCAAGTGAAGCTTTCGTGG
AGTCGGCAGGGCCTGATTGCATCGTTCCAGGCCAAATGAGGCCCATCAAGCTGCTTGGATTACAGCTGTGGCCGATTGAGATCAATTGGAAATTTATGAAACCGGTTGCACGAGAGCTGAAAACAGTAGGCAAGCTTGTTGATTCAGCGTTTGATCTGATGCAAGCCCCTTTTGGTGACCGATAGTTGCCTATTCAACTT
TGATGTCTACTTTGTATTGGCAAGGCTCGAGCGAGAACACATCACCTCACCATTTTCTAATGTTTCTAAATAGAAAGCCACATGATAAAAGGGAACATACTGGTTCTCCAAATCTTCATTCGCACTTGCATAGCTACAAGCACCTAACTCAACTCATGGCAGGAAATAACAAATAATAAAAGTTCAATCTCTTGCAAATA
CAGATGACACTACAGAGCTATAGTGTATTTTTTATCAGTAGTTCGATATATTCCCATTTTTTTTATGGGAAGGTTGTTTCTTATTGCCAAGCATATATAAAGAGTTATGGAAACTGTTTTGAGCTATAATTAAGAGCAAAGGGGTGCAGGTCTACATGAATTCAATGGAAACCCCATCTCTTTCTGTGCAATCTATTGCT
CTTTGGGAATTTATCTGTGCACCACACACTGCAGTGGGCCTCAGATTGTCCTCTAAACTTCTGAAATGTTATAATTTTTTTGTGTCATACAACCTATAAGTCGAGGTTTGTTGAAGAGCTGGAATAAAGTGTTATCTGCAAAATAGTAACAAAGTATTTTACGATTTTCACTTTGTCTTATTGTAATAGCCACTTCTAGG
TTGAAGTCTTCAAATGTAAAAATCTAGTCATTGATTTCATCTGAAAATATGCTCGTGTTGGGACATTTCCAAGAATTCACATCTTTGTTGTGACAAAATAATTTCTTGGAAGACCAGCATATAATTCCATATCTATGCACTACCAGAAATACATAGAATTCCATTTCTATGCACTACCTAGAGTCTCCTTTGATGGAAGA
CAATAAGTTCATTTGCTTAGTTTGTGTTTATCCTCCTGCAAGGGCATGCCAGTTTACACGCAAGGAAACAATTCATGTTAATAGGTAGTCTGTTATGAAATTCTTATTTACCAAACTCATTATGCATGTCCAGTGTACAAGTTTGTTTTCAATAATTTATCAAAATGTAGGTGCA

>TRINITY_DN37376_c0_g1_i1 from sample A6 / Example unspliced isoform:

TTCTAACTATACAACTATCTATGGACGGAAGAGCGCCCATTATCGGATTGGATTGGATTGAATGTGACCAGTATTGGTTCATATCTTCTTCCTTATAAATCGAATATCTCTCATTTCCATTCAATCTCCTTTCTTTTTCTCTATCCATATAGCATAATCCCTTCTAAACTAAACTAATGGTGACGGACTTGTAATTCTGA
CTTGCTTTCCTTTCATTTTGCAATTTCCCCTTTGCTATTCTTGGGAGTCGGGACAAGAATTATCAGCCATCCTCTTCGTCCTTGTGGTGATTACTTGATATGGCTTCCGGGAGAAAATTATTGGCAGAAGATGTGCCATGGAGGGCGACTGCGAAAGGGAGGCCCGTTCCCAGAATATCTCAAGCGCCTCTCCTCGCTGT
TCGCCACTCTCCCAATTCAAATTACGCCATCAGCATCATGAAGCATCCGGACCCCATTGGGAAGGGACTGGCAAGTGAAGCTTTCGTGGAGTCGGCAGGGCCTGATTGCATCGTTCCAGGCCAAATGAGGCCCATCAAGCTGCTTGGATTACAGCTGTGGCCGATTGAGATCAATTGGAAATTTATGAAACCGGTTGCAC
GAGAGCTGAAAACAGTAGGCAAGCTTGTTGATTCAGCGTTTGATCTGATGCAAGCCCCTTTTGGTGACCGATAGTTGCCTATTCAACTTTGATGTCTACTTTGTATTGGCAAGGCTCGAGCGAGgtagtgattttaagcctaggcatcctcttcaccatgtaacatgtgcattatatatgagatatttattgtagttttc
acaagctataagtcatctttatctatactgccatggaataatttcttagaaaagaggtgattacaagaggaatgtaaacgtgatttgagtttgtgcaatgttgtcaaaaattgaatgataacttgataagaaatgaaaagttttcggtttgccaaaaaaatcagtatagaaggagcatatgatttaaatacacaggtcta
aagatatgctttcaatatgtgcagAACACATCACCTCACCATTTTCTAATGTTTCTAAATAGAAAGCCACATGATAAAAGGGAACATACTGGTTCTCCAAATCTTCATTCGCACTTGCATAGCTACAAGCACCTAACTCAACTCATGGCAGGAAATAACAAATAATAAAAGTTCAATCTCTTGCAAATACAGATGACACT
ACAGAGCTATAGTGTATTTTTTATCAGTAGTTCGATATATTCCCATTTTTTTTATGGGAAGGTTGTTTCTTATTGCCAAGCATATATAAAGAGTTATGGAAACTGTTTTGAGCTATAATTAAGAGCAAAGGGGTGCAGGTCTACATGAATTCAATGGAAACCCCATCTCTTTCTGTGCAATCTATTGCTCTTTGGGAATT
TATCTGTGCACCACACACTGCAGTGGGCCTCAGATTGTCCTCTAAACTTCTGAAATGTTATAATTTTTTTGTGTCATACAACCTATAAGTCGAGGTTTGTTGAAGAGCTGGAATAAAGTGTTATCTGCAAAATAGTAACAAAGTATTTTACGATTTTCACTTTGTCTTATTGTAATAGCCACTTCTAGGTTGAAGTCTTC
AAATGTAAAAATCTAGTCATTGATTTCATCTGAAAATATGCTCGTGTTGGGACATTTCCAAGAATTCACATCTTTGTTGTGACAAAATAATTTCTTGGAAGACCAGCATATAATTCCATATCTATGCACTACCAGAAATACATAGAATTCCATTTCTATGCACTACCTAGAGTCTCCTTTGATGGAAGACAATAAGTTCA
TTTGCTTAGTTTGTGTTTATCCTCCTGCAAGGGCATGCCAGTTTACACGCAAGGAAACAATTCATGTTAATAGGTAGTCTGTTATGAAATTCTTATTTACCAAACTCATTATGCATGTCCAGTGTACAAGTTTGTTTTCAATAATTTATCAAAATGTAGGTGCA

Intron 3 GTACTTCAAT ... GAAATTAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	29.41	6	5	0
A2	48.84	11	10	11
A3	34.88	14	10	5
A4	44.19	12	18	1
A5	42.86	8	10	2
A6	32.88	49	35	13
A7	43.2	48	49	24
A8	42.48	44	42	23
A9	34.38	63	49	17
A10	34.75	46	39	10

>TRINITY_DN107510_c0_g1_i14 from sample A3 / Example spliced isoform

AAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGGAATTTTCTGTAAGCAGTGGAGGATTTTTACTATGATTTGAGGAACCGAGGGATTTATACAAGGAGTTG

>TRINITY_DN107510_c0_g1_i16 from sample A3 / Example unspliced isoform:

AAAGAATATAAAGTGATAGAGACCAAAGGCAAAGGCAAGGCAAGGCAAGGCAAGGCCAGGAATGGATTTAAGAGCCACTCAAGCCGTCCATACATTTGTGCTCTAAAATTCCATTTCTTCGCCAATGACCTTATAACTCCTCTTTGGTCATTCATTTTCCAAGTGCCCTGGAATACGGGGTATTTATTTCAGCAAAGGCC
ATTATTGCTTGCAATTCCTCCGTGATAATAATGGAAGCCGAGTTCTGGACTTCTCGCATGACCGCTGCTAAGCGCCAAGCTGCGTTGAACTCAGgtacttcaatttgtctccgctaaattgtcgaatttttctttaatttttttcccctgtgcctcgcattttgcaaattttctcaggaattctgaaggttttttggtga
agtggagaaattaaagGAATTTTCTGTAAGCAGTGGAGGATTTTTACTAGGATTTGAGGAACCAAGGGAATTTATACAAGGAGTTGAGAAAATAGAGGAATTTTCTAGAAAGTCTGGAAAGTTTTCTGTTTTGATTGTAGGTTATGCTAAGTCAACTTTTAAGAAAGAATCTCTGCAGTACAGCGGCTTATTTTGAGATA
AATGTTCATGAATTTAGGGAAGATGCTCTGTTGAGTACACATGATTATTTTGAGCG