sp|Q9XF47|F16P2_MUSAC ----> Fructose-1,6-bisphosphatase, cytosolic

Intron 1 GTAAAGATTA ... TTGCTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	28.42	34	10	17
A2	30	35	10	20
A3	11.11	28	5	2
A4	18.75	26	9	3
A5	15.79	16	2	4
A6	10.69	71	10	7
A7	16.8	52	7	14
A8	14.89	40	4	10
A9	10.49	64	9	6
A10	12.82	34	2	8

>TRINITY_DN129737_c4_g2_i7 from sample A1 / Example spliced isoform

ATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATAAACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCC
GCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTCTCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTA
TTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAATATTGATTGTGGAGTATCGATTGGAACAATATTTGGAATATACATCCGCAAGGATGATAGTGAAGG
GACCTTGAATGATGTGCTTCAGCCAGGAAAAAACATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTACTGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGAATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAG
GCAATACAAAAAACTGGGACGAGCCTACCAAGAAGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGATATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATGGTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAA
GTGTTTCCCATGTCCTTTTTAATGGAGCAAGCAGGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATTCATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAAGAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGGTTCTTTGTTTTCCACATGT
ATTATACCTTTTTCATGACTGGTTTAAGAGTGCAGTCATGGGGTTTTGAATACTTACTTTATAAACTCAAGTTCCTCAAAATTTCAAATACAAGTTATGTGAAAAGCTTGTAGAAGTTGCCAGCTTTAGACAGTTTGGTAAGCTGTGGAGATTCTCCAGTCCCACTAAAATTCGAATTATATAATAGCCCTTTGGCTGTT
GGTTGCAGTGCAAAGCATATATAGCTG

>TRINITY_DN129737_c4_g2_i5 from sample A1 / Example unspliced isoform:

ATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATAAACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCC
GCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTCTCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTA
TTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAATATTGATTGTGGAGTATCGATTGGAACAgtaaagattacattcaagtccctctccaagattattca
ttaaatgttgcaatttcatacttttttatatgaatgctgcatgtgtaatttaatacccttgttgtgagtctggaatattgatgccttatttttccttttcatgataatttctagatgcctgccactatgaagcatgaatggcatggaatagtttctattccagtgttctactgatgtagacctactttgtaatgaatagc
agatacattattgcttgttttccgaaactactttttgatccagcctcaaaatgcatgcatggttcttttttctgccttggaaaggacgaaaggaggccacttatgaagctgttgtttgattgcttgtagATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAA
CATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTACTGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGAATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGA
AGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGATATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATGGTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAAGTGTTTCCCATGTCCTTTTTAATGGAGCAAGCA
GGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATTCATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAAGAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGGTTCTTTGTTTTCCACATGTATTATACCTTTTTCATGACTGGTTTAAGAGTGC
AGTCATGGGGTTTTGAATACTTACTTTATAAACTCAAGTTCCTCAAAATTTCAAATACAAGTTATGTGAAAAGCTTGTAGAAGTTGCCAGCTTTAGACAGTTTGGTAAGCTGTGGAGATTCTCCAGTCCCACTAAAATTCGAATTATATAATAGCCCTTTGGCTGTTGGTTGCAGTGCAAAGCATATATAGCTG

Intron 2 GTATGATCAT ... TTTCTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.26	27.7	13	11
A2	29.17	23	6	12
A3	23.01	31.3	5	13
A4	19.49	29.3	6	8
A5	11.11	16.7	1	3
A6	16.02	40.3	4	11
A7	16.93	58.3	14	9
A8	9.28	39.7	2	6
A9	19.78	53.7	14	12
A10	18.75	35.3	10	6

>TRINITY_DN102740_c5_g1_i6 from sample A4 / Example spliced isoform

AAAAAATTTGATTTGGAGGCTTCTGTGGCCATCCGGGGAGCTTGTGCTACCCCCTTTTGCTTTCTACAATTATTGCAGGACTTGCTAAGCTAATTGGTCTCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTATTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGC
ATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAATATTGATTGTGGAGTATCGATTGGAACAATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAACAT
GGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTACTGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGAATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGAAGT
ATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGATATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATGGTGGCATATTCATGTACCCTGCAGATAAGAAGAGTC

>TRINITY_DN102740_c5_g1_i3 from sample A4 / Example unspliced isoform:

AAAAAATTTGATTTGGAGGCTTCTGTGGCCATCCGGGGAGCTTGTGCTACCCCCTTTTGCTTTCTACAATTATTGCAGGACTTGCTAAGCTAATTGGTCTCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTATTCATCAAGGCCCTCATAAGCAGTGGACGTACGgta
tgatcattttattgagaaaaattgtagtgtgaattattccattctgttggacctcccaaatatatttattcttaatatacttaattgactgcctgatctattggtgccccagtgtatcattgtctttccaatcccttttaaaccatcttgtggcagaaaaatgtagggtgactgttccatttcttgcagAGCATTCTTGT
TTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAATATTGATTGTGGAGTATCGATTGGAACAGTAAAGATTACATTCAAGTCCCTCCC

Intron 3 GTTCTTATGT ... CTAAAAACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	26.42	39	6.4	14
A2	23.08	40	14.4	12
A3	42	29	9.4	21
A4	33.33	24	2.6	12
A5	36.84	12	1.1	7
A6	26.47	50	7.5	18
A7	20.41	78	11.6	20
A8	28.07	41	4.5	16
A9	18.84	56	8.3	13
A10	20.63	50	8.5	13

>TRINITY_DN111687_c12_g1_i9 from sample A3 / Example spliced isoform

CTGAAATTTTGCCTCAGAACGCAGGTGCTCTCCGCTGATTCCAAGGATAAACAACGTGACGTGGCATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATA
AACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCCGCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTC
TCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTATTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAAT
ATTGATTGTGGAGTATCGATTGGAACAATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAACATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTACTGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGA
ATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGAAGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGATATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATG
GTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAAGTGTTTCCCATGTCCTTTTTAATGGAGCAAGCAGGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATTCATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAA
GAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGTGCAAAGCATATATAGCTGTAAAGGATTGTCTCTGACAATGTTGAAGGAGGCTGAAATTTTCATCCATGATAATACCTCTGTTATTAATCTCATGTTCTCTCTCCCGGGTAAGCACTTGCTTATGTTGAAGTTGGTACATAGGTAATTTTGGAGA
TATAGGGTAACTAACCTTTTATTGTTGGTAATAGTATTAGAGATATATATGGATTTACATATATTGAGTGTGAT

>TRINITY_DN111687_c12_g1_i6 from sample A3 / Example unspliced isoform:

CTGAAATTTTGCCTCAGAACGCAGGTGCTCTCCGCTGATTCCAAGGATAAACAACGTGACGTGGCATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATA
AACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCCGCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTC
TCGCGGGAGAGATCAATGTGCAGgttcttatgttaactgagaattagtttcaatttttgaatatcatgtcaatcttattacagttaggtggccttaaatatgtagtatgcagaataatccctcatactacagttgattcacaattgtgatttgaagtttccatattaatctatatgtattaggtgttagaatggatactt
gtgacacaggaattcaatgcttgttactcagctgtgagattcccattgatagttgtatctaaaaacagGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTATTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGA
GGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAATATTGATTGTGGAGTATCGATTGGAACAATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAACATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTAC
TGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGAATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGAAGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGAT
ATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATGGTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAAGTGTTTCCCATGTCCTTTTTAATGGAGCAAGCAGGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATT
CATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAAGAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGGTTCTTTGTTTTCCACATGTATTATACCTTTTTCATGACTGGTTTAAGAGTGCAGTCATGGGGTTTTGAATACTTACTTTATAAACTCAAGTTCCTCAAAATTTCAAATA
CAAGTTATGTGAAAAGCTTGTAGAAGTTGCCAGCTTTAGACAGTTTGGTAAGCTGTGGAGATTCTCCAGTCCCACTAAAATTCGAATTATATAATAGCCCTTTGGCTGTTGGTTGCAGTGCAAAGCATATATAGCTGTAAAGGATTGTCTCTGACAATGTTGAAGGAGGCTGAAATTTTCATCCATGATAATACCTCTGT
TATTAATCTCATGTTCTCTCTCCCGGGTAAGCACTTGCTTATGTTGAAGTTGGTACATAGGTAATTTTGGAGATATAGGGTAACTAACCTTTTATTGTTGGTAATAGTATTAGAGATATATATGGATTTACATATATTGAGTGTGAT

Intron 4 GTTCTTTGTT ... TTGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	56.52	5	10	3
A2	59.5	7.5	16	6
A3	66.67	2	6	2
A4	46.15	7	11	1
A5	-1	0	0	0
A6	69.21	32.5	73	73
A7	66.91	29.5	62	57
A8	71.61	29	63	82
A9	73.27	22	64	56
A10	56.92	14	22	15

>TRINITY_DN111687_c12_g1_i9 from sample A3 / Example spliced isoform

CTGAAATTTTGCCTCAGAACGCAGGTGCTCTCCGCTGATTCCAAGGATAAACAACGTGACGTGGCATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATA
AACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCCGCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTC
TCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTATTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAAT
ATTGATTGTGGAGTATCGATTGGAACAATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAACATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTACTGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGA
ATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGAAGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGATATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATG
GTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAAGTGTTTCCCATGTCCTTTTTAATGGAGCAAGCAGGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATTCATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAA
GAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGTGCAAAGCATATATAGCTGTAAAGGATTGTCTCTGACAATGTTGAAGGAGGCTGAAATTTTCATCCATGATAATACCTCTGTTATTAATCTCATGTTCTCTCTCCCGGGTAAGCACTTGCTTATGTTGAAGTTGGTACATAGGTAATTTTGGAGA
TATAGGGTAACTAACCTTTTATTGTTGGTAATAGTATTAGAGATATATATGGATTTACATATATTGAGTGTGAT

>TRINITY_DN111687_c12_g1_i6 from sample A3 / Example unspliced isoform:

CTGAAATTTTGCCTCAGAACGCAGGTGCTCTCCGCTGATTCCAAGGATAAACAACGTGACGTGGCATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATA
AACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCCGCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTC
TCGCGGGAGAGATCAATGTGCAGGTTCTTATGTTAACTGAGAATTAGTTTCAATTTTTGAATATCATGTCAATCTTATTACAGTTAGGTGGCCTTAAATATGTAGTATGCAGAATAATCCCTCATACTACAGTTGATTCACAATTGTGATTTGAAGTTTCCATATTAATCTATATGTATTAGGTGTTAGAATGGATACTT
GTGACACAGGAATTCAATGCTTGTTACTCAGCTGTGAGATTCCCATTGATAGTTGTATCTAAAAACAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTATTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGA
GGAAGGTACTGTGTAGTTTTTGATCCACTTGATGGCTCCTCCAATATTGATTGTGGAGTATCGATTGGAACAATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAACATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTAC
TGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGAATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGAAGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGAT
ATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATGGTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAAGTGTTTCCCATGTCCTTTTTAATGGAGCAAGCAGGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATT
CATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAAGAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGgttctttgttttccacatgtattatacctttttcatgactggtttaagagtgcagtcatggggttttgaatacttactttataaactcaagttcctcaaaatttcaaata
caagttatgtgaaaagcttgtagaagttgccagctttagacagtttggtaagctgtggagattctccagtcccactaaaattcgaattatataatagccctttggctgttggttgcagTGCAAAGCATATATAGCTGTAAAGGATTGTCTCTGACAATGTTGAAGGAGGCTGAAATTTTCATCCATGATAATACCTCTGT
TATTAATCTCATGTTCTCTCTCCCGGGTAAGCACTTGCTTATGTTGAAGTTGGTACATAGGTAATTTTGGAGATATAGGGTAACTAACCTTTTATTGTTGGTAATAGTATTAGAGATATATATGGATTTACATATATTGAGTGTGAT

Intron 5 GTACTGTGTA ... TTGCTTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	24	24	17
A2	58.97	16	23	20
A3	74.36	10	29	2
A4	62.22	17	28	3
A5	65.38	9	17	4
A6	69.09	17	38	7
A7	60.32	25	38	14
A8	50.94	26	27	10
A9	63.24	25	43	6
A10	61.76	13	21	8

>TRINITY_DN129737_c4_g2_i2 from sample A1 / Example spliced isoform

ATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATAAACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCC
GCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTCTCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTA
TTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAACATGGTTGCAGCAGGATACTGTATGTATGGAAGC
TCTTGCACGGTAATAGTCTTCACCTTGATGATGTACAGTAGGCAGAAAGATGTTTCGGAAAATTTTGACATATGTTCGTGAACATTTTGATGGTATAGAACAGGAAGAAGGATTGTTAGTATTCATATATTCTCGATGTATGTTCCTTGAGTAGTTTGCAT

>TRINITY_DN129737_c4_g2_i5 from sample A1 / Example unspliced isoform:

ATTCCACGTCAGCATGGAACTCCTTTACGCAATGTTGAAATCGAATAAAACTACACTGCACTAGGCTCTCTGAAATTTACAGAACCGAGGCACACGGGCTTATATCGGATCAGCTCAGCTCATTCCATCTCAATAAACACAACTGGGAAGGATGGATCACGCGGCGGAAGCCCATCGAACAGACTTGATGACTATTACCC
GCTTTGTGCTGAATGAACAGTGCAAGCATCCCGAAGCTCGCGGGGATTTCACAATTCTGCTGAGCCATATTGTCCTTGGCTGCAAATTCGTATGCTCTGCTGTCAGCAAGGCAGGACTTGCTAAGCTAATTGGTCTCGCGGGAGAGATCAATGTGCAGGGAGAGGATCAAAAGAAATTAGACGTACTATCAAATGAAGTA
TTCATCAAGGCCCTCATAAGCAGTGGACGTACGAGCATTCTTGTTTCTGAGGAGGATGAACATGCAACCTTTGTTGAGCCATCAAAGCGAGGAAGgtactgtgtagtttttgatccacttgatggctcctccaatattgattgtggagtatcgattggaacagtaaagattacattcaagtccctctccaagattattca
ttaaatgttgcaatttcatacttttttatatgaatgctgcatgtgtaatttaatacccttgttgtgagtctggaatattgatgccttatttttccttttcatgataatttctagatgcctgccactatgaagcatgaatggcatggaatagtttctattccagtgttctactgatgtagacctactttgtaatgaatagc
agatacattattgcttgttttccgaaactactttttgatccagcctcaaaatgcatgcatggttcttttttctgccttggaaaggacgaaaggaggccacttatgaagctgttgtttgattgcttgtagATATTTGGAATATACATCCGCAAGGATGATAGTGAAGGGACCTTGAATGATGTGCTTCAGCCAGGAAAAAA
CATGGTTGCAGCAGGATACTGTATGTATGGAAGCTCTTGCACGCTTGTCTTGAGTACTGGAACTGGAGTAAATGGTTTTACACTTGACCCCTCTCTCGGGGAATTCATTTTGACTCATCCAGACATAAAGGTTCCAAAGAAAGGTAAAATTTATTCTGTGAATGAAGGCAATACAAAAAACTGGGACGAGCCTACCAAGA
AGTATGTTGAGAGATGCAAGTTTCCTGAAGATGGATCATCTCCAAAGTCTCTTAGATATATTGGAAGCATGGTAGCTGATGTCCACAGAACACTTCTATATGGTGGCATATTCATGTACCCTGCAGATAAGAAGAGTCCCAATGGGAAGTTACGTACCCTCTATGAAGTGTTTCCCATGTCCTTTTTAATGGAGCAAGCA
GGAGGTCAAGCCTTTACAGGCACAGGACGAGCACTTGATATAGTTCCAACCAATATTCATCAACGTTCTCCTATCTTCCTTGGGAGCTTTGAGGAAGTAGAAGAGATCAAATCTTTATATGCTGCTCATGAAGCAAAAACCTTATAGGTTCTTTGTTTTCCACATGTATTATACCTTTTTCATGACTGGTTTAAGAGTGC
AGTCATGGGGTTTTGAATACTTACTTTATAAACTCAAGTTCCTCAAAATTTCAAATACAAGTTATGTGAAAAGCTTGTAGAAGTTGCCAGCTTTAGACAGTTTGGTAAGCTGTGGAGATTCTCCAGTCCCACTAAAATTCGAATTATATAATAGCCCTTTGGCTGTTGGTTGCAGTGCAAAGCATATATAGCTG