sp|P14766|F16P2_SPIOL ----> Fructose-1,6-bisphosphatase, cytosolic

Intron 1 GTAAGCATGC ... TTGTTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	9.84	35.6	3.9	1.6
A2	6.49	56.3	4.8	1.8
A3	6.92	38.6	3.6	0
A4	7.94	24.2	2.7	0
A5	11.14	21.7	3.9	0.6
A6	7.22	97	9.6	0.6
A7	7.01	117.4	11.1	0
A8	7.76	64.2	5.9	2
A9	11.04	117.8	16	0.6
A10	5.26	122.2	7.4	0

>TRINITY_DN129737_c4_g1_i4 from sample A1 / Example spliced isoform

ACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGGATGCAA
GTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAA
AGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGATTGATCAC
TATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCTTGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTG
ACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTT
CATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGG
CTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTA
ATCTGTAATGTCGCTGAATTCTTATATATTTC

>TRINITY_DN129737_c4_g1_i6 from sample A1 / Example unspliced isoform:

ATTGATCACTATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCTTGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAA
TTCATTTTGACTCATCCTGACATAAAGGTAACGTAATTCTAATTTTAATTTGGTTCTGAAAATGCAAAATGAATTCAAAAGAAACATGGCTTTCTCACTATCTAATTAGGACGTTTTACATGGATCTGAGTGCATTGTTGAGTACTTGTTCAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAA
GAACTGGGATGAGCCAACCAGGAAgtaagcatgcatgatgattgatggagatagtttttatatttggacaaaattcacatacaattttttccaatacccaccattgtttgcagGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGGTGCTCTGTTAATTTTAATAT
TCCTTCCTGATAGTTTTGACAACTCTGTTTCTAAGCATGCCATGTTTGTTTGTCAGTAAATATTAGAGGACAGGACTTCCTGCATAAATCCTAA

Intron 2 GTATGTAAAT ... ATGCATGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	6.67	28	0	2
A2	21.62	29	0	8
A3	38.89	11	2	7
A4	18.18	18	2	4
A5	28.57	5	0	2
A6	28.13	115	0	45
A7	27.19	83	0	31
A8	28	90	0	35
A9	26.53	108	1	39
A10	21.15	41	0	11

>TRINITY_DN99323_c1_g1_i3 from sample A4 / Example spliced isoform

CTTCTTCTTACCTGTGAGTTTCCTTCTCTTCTTCTTACCTGTGAGTTTCACCTCCTGCAATGAGTGAGTTTCCTTCTCTTCTTCTTTCCTGTGAGTTTCACCTCTTGGAATGAATTATAAAACTAAAAGAACTATTGAAACTCATGAGAAATTGTTATCTACTAAATGATACCCATAGGAAATACATTATCTTGTCTACA
GAGATGGATTATATATGAATAACTTTAAATTCTCAAAGCATTTAATGGGCAAAGCACTCACATCTAATCCTGACTCCTGAATATGCAGGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTG
AGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGGTAATAAATCTCCTCTTGAAGACACAGTTACCCTCCATCTATATATCGTGATGGAATAAATATTTTATTTAAGGAATTTGGTTGGTTAATGTAAGAATTATCTCAAAGAAAAAGTGGGTAGTTATTGGTTG
GAAAGTAAATATACATGTTCGTTTGACTTTCTTCTTTTCTGAAATTGGTTCACAAATAAACAGTAAAGTGAGGCCTACAATTAATTTCTAAAACTTTAAGATCTAATATATTCTGCTCTTCTGGTCTTCTAACTTCACTTTTTCTTAGATTGATCACTATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCT
TGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACT
CGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTTCATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGT
GTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGA
TGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTAATCTGTAATGTCGCT

>TRINITY_DN99323_c1_g1_i5 from sample A4 / Example unspliced isoform:

GAAACATACGTgtatgtaaattttaatagttttagtagcctaaaatgaattataattaattgtatgggggcttagcacaaactacttccatgcatgcagGCACTTGATCTGGTTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGA
ATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTAATCTGTAATGTCGCT

Intron 3 GTGAGTCCGT ... AATGTGGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	3.86	37.5	2	1
A2	0	33.5	0	0
A3	2	49.3	1	1
A4	12.2	36.3	4	6
A5	0	20.8	0	0
A6	0	41	0	0
A7	0	52.3	0	0
A8	0	19.8	0	0
A9	0	51.5	0	0
A10	0	36.5	0	0

>TRINITY_DN99323_c1_g1_i7 from sample A4 / Example spliced isoform

GACAAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGAATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCT
CGGATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGG
AGCATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACG
CTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCC
CAAGTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTTCATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGG
TTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAA
AATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTAATCTGTAATGTCGCT

>TRINITY_DN99323_c1_g1_i2 from sample A4 / Example unspliced isoform:

GACAAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGAATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCT
CGGATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGTGCCCTATTTTTTTTTGCTTATTATTGAATCTCCACCAGTAGAAGATGATGCCCTCCGTCTTCTTAGTTTTCCAACTTTAAATTCAGAATTTCATTATCCCATATTTACTTTTGGGG
TTGCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGgtgagtccgtatcagaaaaaaattattcagaattgaaataccacagtcctttgaaaatgttatttaattttggcaccatttttaatgtggcagTGCATTTTAGTTTCAGAAGAGGATGA
AGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGT

Intron 4 GTGGGTATTT ... TTTTCTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	25.93	50	14	21
A2	20	36	14	4
A3	24.21	36	14	9
A4	18.99	32	6	9
A5	40.91	13	12	6
A6	23.35	64	19	20
A7	22	39	9	13
A8	22.22	42	12	12
A9	18.4	51	12	11
A10	23.64	42	17	9

>TRINITY_DN99994_c0_g1_i4 from sample A5 / Example spliced isoform

CAAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCG
GATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAG
CATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGCT
TGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCA
AGTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTTCATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTT
CCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGA
TG

>TRINITY_DN99994_c0_g1_i1 from sample A5 / Example unspliced isoform:

CAAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCG
GATGCAAGTTTGTATGCTCTGCCGTCAACAAGgtgggtatttttcatatgcagatcatcaatcatttgaatcttcaatttcctgtctcaaccttcgtatttttctgcagGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTAT
TTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGT

Intron 5 GTAATAAATC ... TGGTGTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	30.3	46	15	25
A2	32.14	38	18	18
A3	32.88	36.3	12.5	23
A4	38.18	17	7	14
A5	38.18	17	13	8
A6	22.36	92	10	43
A7	20.62	102	10	43
A8	27.74	56	10	33
A9	21.91	98	6	49
A10	21.08	88	8	39

>TRINITY_DN109590_c0_g1_i2 from sample A2 / Example spliced isoform

CAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGAATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGGATG
CAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATT
CAAAGCGGGGAAAGTTCTTCCCGCGTTCAATCTGCAGTAGTTTTATGATAAGTTCGTTCAATAAGAAGCACTCTGCAGCAGTGCTTTCAGTAGCATCATTAAACGTTAAAGCAGTAGCTTATTAAACCCAGCAGCAATGCTGAAAGCAGCATCATTAAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAAC
ATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGA
ATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGGTGCTCTGTTAATTTTAATATTCCTTCCTGATAGT
TTTGACAACTCTGTTTCTAAGCATGCCATGTTTGTTTGTCAGTAAATATTAGAGGACAGGACTTCCTGCATAAATCCTAAGAACCATGATTTGGAATTCAATATATTTTCTT

>TRINITY_DN109590_c0_g1_i8 from sample A2 / Example unspliced isoform:

CAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAAT
TGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGgtaataaatctcctcttgaagacacagttaccctccatctatatatcgtgatggaataaatattttatttaaggaattt
ggttggttaatgtaagaattatctcaaagaaaaagtgggtagttattggttggaaagtaaatatacatgttcgtttgactttcttcttttctgaaattggttcacaaataaacagtaaagtgaggcctacaattaatttctaaaactttaagatctaatatattctgctcttctggtcttctaacttcactttttcttag
attgatcactatatctgcaactgacttgagataaggattcttgctttattcttgttatacaattctaagtatgaactttgttttagttagttttactgtcattaaattattcatagtgacagctagtttctggtgtgtagCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAA
TTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGGTGCTCTGTTAATTTTAATATTCCTTCCTGATAGTT
TTGACAACTCTGTTTCTAAGCATGCCATGTTTGTTTGTCAGTAAATATTAGAGGACAGGACTTCCTGCATAAATCCTAAGAACCATGATTTGGAATTCAATATATTTTCTT

Intron 6 GTGCCCTATT ... GGGGTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	18.33	49	11	6.1
A2	27.66	34	13	8.1
A3	21.54	51	14	14.4
A4	29.17	34	14	6
A5	0	17	0	3.4
A6	20	56	14	4.7
A7	9.09	50	5	4.7
A8	23.91	35	11	2.6
A9	17.81	60	13	5.1
A10	27.45	37	14	3.9

>TRINITY_DN111687_c12_g2_i5 from sample A3 / Example spliced isoform

AAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGG
ATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGC
ATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGCTT
GTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAA
GTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTTCATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTTC
CCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAAT
TAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTAATCTGTAATGTCGCTGAATTCTTATATATTTCTAGTATGCATTATATGTGCGTTTGTAAATGCATATAGATGATGTCCATTACAAGTCTCCATTTTAATGAAAAAATCTAATATCAATCGGTATATGATTCTTT
TCTACTGAAATTGAACTTTATTATGTACTAGCTCTGACACCATGTATGACAAAGCTATGAAACGCTAACTAGGAGCGAAGAGAATAAAG

>TRINITY_DN111687_c12_g2_i1 from sample A3 / Example unspliced isoform:

AAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGG
ATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGgtgccctatttttttttgcttattattgaatctccaccagtagaagatgatgccctccgtcttcttagttttccaactttaaattcagaatttcattatcccatatttacttttggggttg
cagGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGTCCTCATTGCTCAACTTTATAATTTCTTCCCTTTTTACGAATTTAATTTAATAT
TTTGGGGGGTTAATTGCATCCAGGTTTTGCTCTTCCGATTTTTATTTATTTTTATTATTGAGAATAGTCCATATGTTATGTTATTTTTACGAATTTAATTTAAGTTTTTTTTGGATTAATGGTTTCTATTCATGGTTTTATGATAGGTTCTTCCCGCGTTCAATCTGCAGTAGTTTTATGATAAGTTCGTTCAATAAGAA
GCACTCTGCAGCAGTGCTTTCAGTAGCATCATTAAACGTTAAAGCAGTAGCTTATTAAACCCAGCAGCAATGCTGAAAGCAGCATCATTAAAAGTACTGCTGCCTGGTTCAGCCCAATACTTAAAAGCTATTCTTCAATTCTTCATTTTTTCATTTTTGATAATAGCCCATACGCTTATTGAACACAATTCTTCAATTTT
TTCATTTTTGAGAATACCCATAAGCTTATTGAACGCCATTCTTCATTTTTTTCATTTTTGAGAATAGCCCATAAGCTATAAGCTAATGTATATAACAAAGTGGATGAAAGAAGTAGGACTGTAATCATCGAACTAAATTCCATATTATTAATATTCACTTTCCTACTTGTAAGATTTTCTTAAATTTAAACGAGTGTTGA
AAGGCGTTCAATAAGCACCCGGCATAACTAATCTTTTCTTGAAGTTTTAATAAGCACCCGGC

Intron 7 GTAATAAATC ... TTTTTCTTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	37.04	17	15	5
A2	37.31	21	18	7
A3	32.89	17	12.7	4
A4	54.84	7	7	10
A5	50	7	13	1
A6	34.88	14	10	5
A7	25.58	16	10	1
A8	28.21	14	10	1
A9	31.25	11	6	4
A10	38.1	13	8	8

>TRINITY_DN109590_c0_g1_i13 from sample A2 / Example spliced isoform

CAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGAATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGGATG
CAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATT
CAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGATTGAT

CACTATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCTTGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATT
TTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGGTGCTCTGTTAATTTTAATATTCCTTCCTGATAGTTTTGACA
ACTCTGTTTCTAAGCATGCCATGTTTGTTTGTCAGTAAATATTAGAGGACAGGACTTCCTGCATAAATCCTAAGAACCATGATTTGGAATTCAATATATTTTCTT

>TRINITY_DN109590_c0_g1_i8 from sample A2 / Example unspliced isoform:

CAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAAT
TGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGgtaataaatctcctcttgaagacacagttaccctccatctatatatcgtgatggaataaatattttatttaaggaattt
ggttggttaatgtaagaattatctcaaagaaaaagtgggtagttattggttggaaagtaaatatacatgttcgtttgactttcttcttttctgaaattggttcacaaataaacagtaaagtgaggcctacaattaatttctaaaactttaagatctaatatattctgctcttctggtcttctaacttcactttttcttag


ATTGATCACTATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCTTGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAA
TTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGGTGCTCTGTTAATTTTAATATTCCTTCCTGATAGTT
TTGACAACTCTGTTTCTAAGCATGCCATGTTTGTTTGTCAGTAAATATTAGAGGACAGGACTTCCTGCATAAATCCTAAGAACCATGATTTGGAATTCAATATATTTTCTT

Intron 8 GTGCTCTGTT ... TTATACGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	21.28	37	17	3
A2	22.52	43	19	6
A3	18.84	28	10	3
A4	25	21	12	2
A5	31.43	12	5	6
A6	15.47	112	20	21
A7	20	118	28	31
A8	13.51	64	13	7
A9	20.55	116	43	17
A10	15.57	141	34	18

>TRINITY_DN42895_c0_g3_i6 from sample A7 / Example spliced isoform

GGAAATTGGGATTTAAGATAAGATGCCCTTCATTTGATTTCATTATTATGTATTTAGACAAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAG
TCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGGATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAG
CAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCA
AACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGAT
GAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTTCATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTT
AATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATG
TGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTAATCTGTAATGTCGCTGAATTCTTATATATTTCTAGTATGCATTATATGTGCGTTTGTAAATGCATATAGATGATG
TCCATTACAAGTCTCCATTTTAATGAAAAAATCTAATATCAATCGGTATATGATTCTTTTCTACTGAA

>TRINITY_DN42895_c0_g3_i5 from sample A7 / Example unspliced isoform:

GGAAATTGGGATTTAAGATAAGATGCCCTTCATTTGATTTCATTATTATGTATTTAGACAAACAAACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAG
TCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGGATGCAAGTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAG
CAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAAAGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCA
AACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTGACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGAT
GAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGgtgctctgttaattttaatattccttcctgatagttttgacaactctgtttctaagcatgccatgtttgtttgtcagtaaatattagaggacaggacttcctgcataaatcctaagaacc
atgatttggaattcaatatattttcttgtcataatctaaatctgtggcttcgtggttttcttatacgcagCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTTCATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAA
GCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGAT
TAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTAATCTGTAATGTCGCTGAATTCTTATATATTTCTAGTATGCATTATATGTGCGTTTGTAAATGCATATAGATGATGTCCATTACAA
GTCTCCATTTTAATGAAAAAATCTAATATCAATCGGTATATGATTCTTTTCTACTGAA

Intron 9 GTAACGTAAT ... ACTTGTTCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	23.96	32.3	10.3	2.8
A2	10.67	46.7	5.7	1
A3	3.92	40.7	1.7	0
A4	14.45	30.3	5.3	6
A5	2.58	24.7	0.7	0.8
A6	12.26	107.7	16.3	0
A7	10.44	127	16	0
A8	8.77	58.9	5.7	0
A9	10.03	115.3	13.7	5.4
A10	13.11	102.2	15.7	5.6

>TRINITY_DN129737_c4_g1_i4 from sample A1 / Example spliced isoform

ACCGCTGGCAAGGTGGGAAGCTGAAATTAATTGAATTTCCTTGGATTGCATTTGTGGGGAAAGAACATGGACCACGAAGCAGATGCCTATCGCACGGATCTCATGACCATTACGCGATTCGTTCTGAACGAGCAGTCCAAGTATCCTGAATCGCGAGGAGATTTCTCCATTTTGCTCAGTCACATTGTTCTCGGATGCAA
GTTTGTATGCTCTGCCGTCAACAAGGCCGGTCTGGCTAAGCTCATTGGCCTTGCAGGAGAGACCAACGTTCAGGGCGAAGAGCAAAAGAAATTAGATGTGCTCTCTAATGAAGTATTTGTCAAGGCTCTTGTCAGCAGTGGGCGCACGTGCATTTTAGTTTCAGAAGAGGATGAAGAGGCCACCTTTGTGGAGCATTCAA
AGCGGGGAAAGTATTGTGTAGTCTTCGATCCCCTGGACGGCTCTTCCAACATTGATTGTGGAGTGTCAATTGGTACGATATTCGGAATATACTTGGTGAAGGATGGAGGAGAGGCATCCTTGAGTGATGTTCTCAAACCAGGCAAGAAAATGGTTGCAGCAGGTTATTGCATGTACGGGAGCTCCTGCACGATTGATCAC
TATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCTTGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAATTCATTTTG
ACTCATCCTGACATAAAGATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAAGAACTGGGATGAGCCAACCAGGAAGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGCATGGTAGCTGATGTGCACAGAACCCTACTCTATGGTGGTATTTT
CATGTACCCTGGAGATAAGAAGAGTCCCAATGGAAAATTGCGTGTTCTGTACGAAGTGTTCCCCATGTCATTCTTAATGGAGCAAGCAGGAGGTCAAGCTTTCACAGGAAACATACGTGCACTTGATCTGGTTCCCACTAAGATTCACCAGCGTTCCCCGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTAAGG
CTCTGTACGCTGCACAGGAATAGGTTTTTCTTGGGAGTTATGATGATGTGGAGGAGATTGGGAGTTATGATGATGTGGAGGAGATTAAGGCTCTGTATTTGCTCAGGATTGATTAACTAATTTTCAAAAAAAATTAAAAAATGCCCATAAGAAAACATTAATTTATCATTTAATGTTATTTTTAAGGGCAAATCATTGTA
ATCTGTAATGTCGCTGAATTCTTATATATTTC

>TRINITY_DN129737_c4_g1_i6 from sample A1 / Example unspliced isoform:

ATTGATCACTATATCTGCAACTGACTTGAGATAAGGATTCTTGCTTTATTCTTGTTATACAATTCTAAGTATGAACTTTGTTTTAGTTAGTTTTACTGTCATTAAATTATTCATAGTGACAGCTAGTTTCTGGTGTGTAGCTTGTGCTGAGCACTGGCAGTGGTGTAAATGGCTTTACATTAGATCCCTCCCTCGGGGAA
TTCATTTTGACTCATCCTGACATAAAGgtaacgtaattctaattttaatttggttctgaaaatgcaaaatgaattcaaaagaaacatggctttctcactatctaattaggacgttttacatggatctgagtgcattgttgagtacttgttcagATACCGAAGAAAGGGAAAATATACTCGGTGAACGAAGGCAATACAAA
GAACTGGGATGAGCCAACCAGGAAGTAAGCATGCATGATGATTGATGGAGATAGTTTTTATATTTGGACAAAATTCACATACAATTTTTTCCAATACCCACCATTGTTTGCAGGTACGTTGAGAGATGCAAATTTCCCCAGGATGGATCATCTCCCAAGTCTCTCAGATATATTGGAAGGTGCTCTGTTAATTTTAATAT
TCCTTCCTGATAGTTTTGACAACTCTGTTTCTAAGCATGCCATGTTTGTTTGTCAGTAAATATTAGAGGACAGGACTTCCTGCATAAATCCTAA