sp|Q20HU6|SELNS_PICSI ----> Delta-selinene-like synthase, chloroplastic

Intron 1 GTGAATGCAA ... GCATTTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	27.59	21	4	8
A2	15.79	16	0	3
A3	0	18	0	0
A4	40	6	3	4
A5	9.09	10	0	1
A6	40.74	16	0	11
A7	14.29	6	0	1
A8	15.38	11	0	2
A9	11.11	16	2	2
A10	20	12	0	3

>TRINITY_DN43117_c0_g1_i13 from sample A9 / Example spliced isoform

TTCATCACTGAGCTCGCTTCGCTGAGCGTAGTTTTATAATAAAGAGAGAGAGAGTTCAATTTGCACAAGTTATGGCCGACATCTCCGAATCTGTTGCCACCATTTTGAACGATGCATCTTCGATCGCTCGACGCACTGCCAATCATCACGGGAATGTCTGGGACGACGCTGTCATACAATCTCTCAATTCCCCCTATGGG
GCACCTGCGTATCATGAACGCGTTGAAAAGCTTATTGTGGAGATCAAGCATTTACTTCTCACTGAAATGGAAGATACCAATCTTGATTTAATCAAACGTCTTGAGATCGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACACGAAATTAAAACAGCTGCTCTCGATTACGTGTACAGATGTTGGAACGA
AAGAGGTATCGGCGTGGGATCAAGAGATTCCCTCACCAAAGATCTTAACGCTACAGCTTTAGGATTTCATGCTCTCCGACTTTATCGATATAACGTATCCGCAGGAGTGTTGGAGAATTTCAAGAATGAGCACGGGAAGTTCTTCTGCAATTTCAGTGGTGATGAACGAGTGAGAAGCATGTTGTCGTTACTTCGGGCTT
CAGAGATTTCATTTCCCGGAGAAAAAGTGATGGAAGAAGCCAAGGCATTCACAAGAGAATATCTAAACCAAGTTTTAGCTGGATTCGGGGATGTGACGGACGTGGATCAAAGCCTTTTGAGAGAGGTGAAGTACGCATTGGAGTTTCCATGGCATTGCAGTGTGCCCAGGTGGGAGGCAAGGAGCTTTATTGAAATCTAT
TCACAAAATCAATCCTGGCTCAAGTCGGAAATCAATCAAAAATTTTTGGAATTAGCCAAATTGGACTTCAATATCCTGCAATGCATCTATCAGAAAGAGACACAGTTTTTTTCAAGGTGGTGGAGCGACTCAGACATAGTGCAGCTGAATTTCTATAGGAAGCGTCACGTGGAATATTATTTTTGGGCGGTTATAGGCGT
TCTCGAGCCCGAATTTTCTCAAAGCAGAATTGCCTTCGCAAAAATAGCTACCGTGGGAACTGTTCTAGATGACATCTGTGATACTCACGGAACTTTGGACGAAGTCAGAACCATCACGGAGGCAGTCAGACGATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATA
CAGCGAATGAATTGGCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGAGTCTTATCTGCAAGAAGTGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCG
CTTCTGTTGATGGGTGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGATCAGACTCACCGACGACTTGAGAGATTTCGAGGAGGAGAAGGAACGCGGGGAGATAGCTTCAATTGTGGAGTGTTATATGAAAGAGAATCCTGGATCTACACTGGAAAATGC
TTTAAATCACATAAAAGACATCCTTCATCAATCCCTAGAGGAATTGAATAGGGAGTTTATGAAGAAGGACAGTGTACCATTGTGTTGCAAGAAGTTCAGTTTCAATATAGCACGAGGCATTCAATTCCTCTACAAATACGGAGACGGCATATGCATTTCTGACAAGGAAGTAAAGGAGCAGATAATCAAAATCCTCGTGG
AGCAAGTGCCTATTGAGGAATACTGATGACCCGGTAGCTGTGTATGGCCATATATTAAATAAGTGGCGCATAATGAGACTTAGAAAGTTTGTAAAAGTGGCCGTAGTTTGTCTAGATTTTGCGAACTTGCCGACGGACATTGTTTCCGCACAAATGACATATATCAAGTCAGGACCGATTTCCTTCTATGTTGTCCCGTG
CTTTATGATATTGAATTAGCAACTTCGTCCTCATGATGGGTGGTTCACAAATATGTAAACTACAAATTCTCTACTAATGTGTAGTATCTTCTTTATCGTCTTTATTCAGGATTGTAATCATAATTTTTATTGACCA

>TRINITY_DN43117_c0_g1_i16 from sample A9 / Example unspliced isoform:

ACCATCACGGAGGCAGTCAGACGgtgaatgcaaattgcgaattgattaatttctacttccgtgcataattgcagagcaccttttatatgaagattttcatttttattgttgattgtggcatttgcagATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATACAGCG
AATGAATTGGCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGAGTCTTATCTGCAAGAAGTGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCGCTTCT
GTTGATGGGTGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGATCAGACTCACCGACGACTTGAGAGATTTCGAGGAGGAGAAGGAACGCGGGGAGATAGCTTCAATTGTGGAGTGTTATATGAAAGAGAATCCTGGATCTACACTGGAAAATGCTTTAA
ATCACATAAAAGACATCCTTCATCATTCCCTTGAGGAATTGAATAGGGAGTTTATGAAGAAGGACAGTGTACCATTGTGTTGCAAGAAGTTCACTTTCAATATAGCACG

Intron 2 GCCCTTGAAT ... TTATCTCAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.67	4	3	4
A2	63.64	2	7	0
A3	44.44	5	4	4
A4	100	0	2	0
A5	-1	0	0	0
A6	25	3	2	0
A7	42.86	12	5	13
A8	38.46	8	5	5
A9	41.33	22	14	17
A10	66.09	5	10.5	9

>TRINITY_DN43117_c0_g1_i4 from sample A9 / Example spliced isoform

CCTTTGGTTTGAGTTTTCATTGGCCCAACGCATGCGCATCTTTCAGGCCAAAGAGATCTGTACCGCGAATAACCAGGAGCTGCATGAGCTTCACCGTTGCAGCCGATGATGCACAAAGACGCATAGGCAATCATCATTCCAACCTCTGGGACGATGATATCATACAATCTCTCTCAACGTCTTCTGATGGGGGACCTGCA
CATCGTGAACGGGTGGAAAAACTTATTGAAGACATCAAAATTCTACTTTCTAGTGACATGGAAGATAGCCGCAGTGATAGTGATGTAGTCGAACGTCTTCAGATCGTTGATACAATCGAATGTCTGGGAATTGATCGACATTTTCAACCTGAAATAAAAGTAGCTATAGATTACGTTTACAGATATTGGAACGAAAGCGG
CATCGCAGAGGGGTCAAGAGATTCCCTCATCAAAGATCTCAACGCTACAGCTTTGGGATTTCGGGCACTCTGATTGCATCGGTATAACGTTTCCTCAGGTGTTTTGGAGAATTTCAGGGATGAAAACGGGCAGTTCTTCTGCAATTTCACCGGTGAAGAAGAAGGGCGGGATGATAAACGAGTGAGAAACATGTTGTCAT
TATTTCGAGCTTCAAACATTTCATTTCCCGGAGAAAAGGTGATGGATGAAGCAAAGACATTCACAACCGAGTATCTAAACCAAGTTTTAACGGGGCAAGATGTAACTGTTGTCGATCAAAGCCTTCTGAGAGAGGTGATGGAGAGAGTCATATCTATCGCAGCTGAATTTCTTTCGGGAGCGTCACGTGGAATCTTACTT
CTGGGTGGTTACAGGCACATTCGAGCCTGAGTTTTCTCGAAGCAGAATTGTTGTCGCAAAACTTGGTACACTGATGACTGTTCTAGATGACCTATTTGGTAGCCTGAGTTTTGCTGCTAATTCCACATGTTAATAATTTAATATTAGTGTTTGAAATAAGATTTAGAATTACTTATATTAATTAAGATTTTATATTTCAT
AATTTTTAGATATAAAAAATAGGTTAATAACTAAGAGCCGGAGGCATTTCGCATGCCATTTAAGGTGT

>TRINITY_DN43117_c0_g1_i1 from sample A9 / Example unspliced isoform:

CGATACCACACCGTTACATTTCTTGTATCCTCCTCGCACAGAGCTGCAGAACGAGAGTGCACAAAATCCTTGGTCATAGAGAGCGCATTTAGGGGCCAGGTCAAAGTAGTAATGGCTCTCGTTTCTGTAGCGGCATTGATTTCCAGATGGGGCGTCAACATATCAATTATCAGCTCCAGGAAAAATCTTACGGCTTTCTG
TAAACCATTTTCAACTATTGGAATGTTTAGGCCAACGAAATCTCTCTCGCAAATAACCAGGAGGTGCATGAGCTGCAGTACCGTTGCAGCTGATGATGCACAAAGACGCATAGGCAATCATCATTCCAACCTCTGGGACGATGATATCATACAATCTCTCTCAACGTCTTCTGATGGGGGACCTGCACATCGTGAACGGG
TGGAAAAACTTATTGAAGACATCAAAATTCTACTTTCTAGTGACATGGAAGATAGCCGCAGTGATAGTGATGTAGTCGAACGTCTTCAGATCGTTGATACAATCGAATGTCTGGGAATTGATCGACATTTTCAACCTGAAATAAAAGTAGCTATAGATTACGTTTACAGATATTGGAACGAAAGCGGCATCGCAGAGGGG
TCAAGAGATTCCCTCATCAAAGATCTCAACGCTACAGCTTTGGGATTTCGGGCACTCTGATTGCATCGGTATAACGTTTCCTCAGGTGTTTTGGAGAATTTCAGGGATGAAAACGGGCAGTTCTTCTGCAATTTCACCGGTGAAGAAGAAGGGCGGGATGATAAACGAGTGAGAAACATGTTGTCATTATTTCGAGCTTC
AAACATTTCATTTCCCGGAGAAAAGGTGATGGATGAAGCAAAGACATTCACAACCGAGTATCTAAACCAAGTTTTAACGGGGCAAGATGTAACTGTTGTCGATCAAAGCCTTCTGAGAGAGgcccttgaatttccatggcattgcaatttgccgagatgggaggcaaggaactttatcgaaatatatacacaaaacgaaa
attatagagttggctaaattggacttcaatattctgcaatgcacacatcagcaagagttgcaaattatctcaagGTGATGGAGAGAGTCATATCTATCGCAGCTGAATTTCTTTCGGGAGCGTCACGTGGAATCTTACTTCTGGGTGGTTACAGGCACATTCGAGCCTGAGTTTTCTCGAAGCAGAATTGTTGTCGCAAA
ACTTGGTACACTGATGACTGTTCTAGATGACCTATTTGGTAGCCTGAGTTTTGCTGCTAATTCCACATGTTAATAATTTAATATTAGTGTTTGAAATAAGATTTAGAATTACTTATATTAATTAAGATTTTATATTTCATAATTTTTAGATATAAAAAATAGGTTAATAACTAAGAGCCGGAGGCATTTCGCATGCCATT
TAAGGTGT

Intron 3 GTGAAGTACG ... GGCATTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	79.59	5	20	19
A2	70	15	32	38
A3	72.73	6	17	15
A4	89.19	2	17	16
A5	69.23	2	5	4
A6	61.4	11	18	17
A7	84.62	1	5	6
A8	70.37	4	9	10
A9	76.47	4	12	14
A10	81.82	1	5	4

>TRINITY_DN112767_c0_g1_i8 from sample A2 / Example spliced isoform

CATATTGTTGAGCCGTACCAAGCGAAATAGTTTAACTGATTAGCTTAGTATAGTTGTAGCTAATCTTATTGGAGATATTGCTAATTCCGTGTTAGATTCGAATCTCTTTGATGAGATGCGTTCAGTAATGTGTTTGGTAAGTTATGTGGTAGCAGAGACTTTTATGTTGAGGTGCACTAGATTGATTGATGATACATCCA
CTAAATGTGCGAAGTATCTTTCCTCAAAATTTTCCTCAAAATTTCCTCAAAATTGATGATACGGGATATTTCCTCAAAATTTTCTTTGGTTTAGTTCGATACTTATAGTACGAAGCTTCGAAACAGGAGTGTTGGAGAATTTCAAGAATGAGCACGGGAAGTTCTTCTGCAATTTCAGTGGTGATGAACGAGTGAGAAGC
ATGTTGTCGTTACTTCGGGCTTCAGAGATTTCATTTCCCGGAGAAAAAGTGATGGAAGAAGCCAAGGCATTCACAAGAGAATATCTAAACCAAGTTTTAGCTGGATTCGGGGATGTGACGGACGTGGATCAAAGCCTTTTGAGAGAGTGTGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATCTATTCACAAAATCAAT
CCTGGCTCAAGTCGGAAATCAATCAAAAATTTTTGGAATTAGCCAAATTGGACTTCAATATCCTGCAATGCATCTATCAGAAAGAGACACAGTTTTTTTCAAGGTGGTGGAGCGACTCAGACATAGTGCAGCTGAATTTCTATAGGAAGCGTCACGTGGAATATTATTTTTGGGCGGTTATAGGCGTTCTCGAGCCCGAA
TTTTCTCAAAGCAGAATTGCCTTCGCAAAAATAGCTACCGTGGGAACTGTTCTAGATGACATCTGTGATACTCACGGAACTTTGGACGAAGTCAGAACCATCACGGAGGCAGTCAGACGATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATACAGCGAATGAATT
GGCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGAGGCTTATCTGCAAGAAGCGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCGCTTCTGTTGATGG
GTGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGATCAGACTCACAGACGACTTGAGAGATTTCGAGG

>TRINITY_DN112767_c0_g1_i12 from sample A2 / Example unspliced isoform:

CGCTTCGCAGAGCGTAGTTTTATAATAAAGAGAGAGAGAGTTCAATTTGCACAAGTTATGGCCGACATCTCCGAATCTGTTGCCACCATTTTGAACGATGCATCTTCGATCGCTCGACGCACTGCCAATCATCACGGGAATGTCTGGGACGACGCTGTCATACAATCTCTCAATTCCCCCTATGGGGCACCTGCGTATCA
TGAACGCGTTGAAAAGCTTATTGTGGAGATCAAGCATTTACTTCTCACTGAAATGGAAGATACCAATCTTGATTTAATCAAACGTCTTGAGATCGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACACGAAATTAAAACAGCTGCTCTCGATTACGTGTACAGGTTACGCATTTCTAAACACATGAAGC
AATTTAATTTAAGCGTTTACATATATCTGTACATTTTTTTTAGTGGTGGTGGGGGGTGTGTTTTATTCGTTCGTTCGAAGCTGACTGATGAGCTGAGCAGATGTTGGAACGAAAGAGGTATCGGCGTGGGATCAAGAGATTCCCTCACCAAAGATCTTAACGCTACAGCTTTAGGATTTCGTGCTCTCCGACTTTATCGA
TATAACGTATCCGCAGGAGTGTTGGAGAATTTCAAGAATGAGCACGGGAAGTTCTTCTGCAATTTCAGTGGTGATGAACGAGTGAGAAGCATGTTGTCGTTACTTCGGGCTTCAGAGATTTCATTTCCCGGAGAAAAAGTGATGGAAGAAGCCAAGGCATTCACAAGAGAATATCTAAACCAAGTTTTAGCTGGATTCGG
GGATGTGACGGACGTGGATCAAAGCCTTTTGAGAGAGgtgaagtacgcattggagtttccatggcattgcagTGTGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATCTATTCACAAAATCAATCCTGGCTCAAGTCGGAAATCAATCAAAAATTTTTGGAATTAGCCAAATTGGACTTCAATATCCTGCAATGCATCT
ATCAGAAAGAGACACAGTTTTTTTCAAGGTGGTGGAGCGACTCAGACATAGTGCAGCTGAATTTCTATAGGAAGCGTCACGTGGAATATTATTTTTGGGCGGTTATAGGCGTTCTCGAGCCCGAATTTTCTCAAAGCAGAATTGCCTTCGCAAAAATAGCTACCGTGGGAACTGTTCTAGATGACATCTGTGATACTCAC
GGAACTTTGGACGAAGTCAGAACCATCACGGAGGCAGTCAGACGATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATACAGCGAATGAATTGGCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGA
GGCTTATCTGCAAGAAGCGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCGCTTCTGTTGATGGGTGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGA
TCAGACTCACAGACGACTTGAGAGATTTCGAGGAGGAGAAGGAACGCGGGGAGATAGCTTCAATTGTGGAGTGTTATATGAAAGAGAATCCTGAATCTACACTGGAAAATGCTTTAAATCACATAAAAGACATCCTTCATCAATCCCTAGAGGAATTGAATAGGGAGTTTATGAAGAAGGACAGTGTACCATTGTGTTGC
AAGAAGTTCAGTTTCAATATAGCACGAGGCATTCAATTCCTCTACAAATACGGAGACGGCATATGCATTTCTGACAAGGAAGTAAAGGAGCAGATAATCAAAATCCTCGTGGAGCAAGTGCCTATTGAGGAATACTGATGACCCGGTAGCTGTGTATGGCCATATATTAAATAAGTGGCGCATAATGAGACTTAGAAAGT
TTGTAAAAGTGGCCGTAGTTTGTCTAGATTTTGCGAACTTGCCGACGGACATTGTTTCCGCACAAATGACATATATCAAGTCAGGACCGATTTCCTTCAAG

Intron 4 GTATCTTGGC ... CTCTAATCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	46.27	18	24	7
A2	26.56	47	21	13
A3	26.03	27	11	8
A4	26.83	15	4	7
A5	33.33	10	3	7
A6	66.67	1	2	2
A7	0	3	0	0
A8	-1	0	0	0
A9	26.32	7	3	2
A10	20	4	1	1

>TRINITY_DN117763_c0_g1_i7 from sample A1 / Example spliced isoform

CGCTTCGCCGAGCGTAGTTTTATAATAAAGAGAGAGAGAGTTCAATTTGCACAAGTTATGGCCGACATCTCCGAATCTGTTGCCACCATTTTGAACGATGCATCTTCGATCGCTCGACGCACTGCCAATCATCACGGGAATGTCTGGGACGACGCTGTCATACAATCTCTCAATTCCCCCTATGGGGCACCTGCGTATCA
TGAACGCGTTGAAAAGCTTATTGTGGAGATCAAGCATTTACTTCTCACTGAAATGGAAGATACCAATCTTGATTTAATCAAACGTCTTGAGATCGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACACGAAATTAAAACAGCTGCTCTCGATTACGTTTACAGATGTTGGAACGAAAGAGGTATCGGCG
TGGGATCAAGAGATTCCCTCACCAAAGATCTTAACGCTACAGCTTTAGGATTTCATGCTCTCCGACTTTATCGATATAACGTATCCGCAGGAGTGTTGGAGAATTTCAAGAATGAGCACGGGAAGTTCTTCTGCAATTTCAGTGGTGATGAACGAGTGAGAAGCATGTTGTCGTTACTTCGGGCTTCAGAGATTTCATTT
CCCGGAGAAAAAGTGATGGAAGAAGCCAAGGCATTCACAAGAGACTATCTAAACCAAGTTTTAGCTGGACTCGGGGATGTGACGGACGTGGATCAAAGCCTTTTGAGAGAGGTGAAGTACGCATTGGAGTTTCCATGGCATTGCAGTGTGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATATATTCACAAAATCAATC
CTGGCTCAAGTCGGAAATCAATCAAAAATTTTTGGAATTAGCCAAATTGGACTTCAATATCCTGCAATGCATCTATCAGAAAGAGACACAGTTTTTTTCAAGGTGGTGGAGCGACTCAGACATAGTGCAGCTGAATTTCTATAGGAAGCGTCACGTGGAATATTATTTTTGGGCGGTTATAGGCGTTCTCGAGCCCGAAT
TTTCTCAAAGCAGAATTGCCTTCGCAAAAATAGCTACCGTGGGAACTGTTCTAGATGACATCTGTGATACTCACGGAACTTTGGACGAAGTCAGAACCATCACGGAGGCAGTCAGACGATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATACAGCGAATGAATTG
GCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGAGGCTTATCTGCAAGAAGCGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCGCTTCTGTTGATGGG
TGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGATCAGACTCACAGACGACTTGAGAGATTTCGAGGAGGAGAAGGAACGCGGGGAGATAGCTTCAATTGTGGAGTGTTATATGAAAGAGAATCCTGAATCTACACTGGAAAATGCTTTAAATCACATAA
AAGACATCCTTCATCAATCCCTAGAGGAATTGAATAGGGAGTTTATGAAGAAGGACAGTGTACCATTGTGTTGCAAGAAGTTCAGTTTCAATATAGCACGAGGCATTCAATTCCTCTACAAATACGGAGACGGCATATGCATTTCTGACAAGGAAGTAAAGGAGCAGATAATCAAAATCCTCGTGGAGCAAGTGCCTATT
GAGGAATACTGATGACCCGGTAGCTGTGTATGGCCATATATTAAATAAGTGGCGCATAATGAGACTTAGAAAGTTTGTAAAAGTGGCCGTAGTTTGTCTAGATTTTGCGAACTTGCCGACGGACATTGTTTCCGCACAAATGACATATATCAAGTCAGGAC

>TRINITY_DN117763_c0_g1_i2 from sample A1 / Example unspliced isoform:

CGCTTCGCCGAGCGTAGTTTTATAATAAAGAGAGAGAGAGTTCAATTTGCACAAGTTATGGCCGACATCTCCGAATCTGTTGCCACCATTTTGAACGATGCATCTTCGATCGCTCGACGCACTGCCAATCATCACGGGAATGTCTGGGACGACGCTGTCATACAATCTCTCAATTCCCCCTATGGGgtatcatggcagct
atctctatcgttgtcatctgctctttgtttgcttttagttttcagtttgtttcaagcgttggcatctatgtttcttgaattgcattgcactctaatcagGCACCTGCGTATCATGAACGCGTTGAAAAGCTTATTGTGGAGATCAAGCATTTACTTCTCACTGAAATGGAAGATACCAATCTTGATTTAATCAAACGTCT
TGAGATCGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACACGAAATTAAAACAGCTGCTCTCGATTACGTTTACAGATGTTGGAACGAAAGAGGTATCGGCGTGGGATCAAGAGATTCCCTCACCAAAGATCTTAACGCTACAGCTTTAGGATTTCATGCTCTCCGACTTTATCGATATAACGTATCCG
CAGGAGTGTTGGAGAATTTCAAGAATGAGCACGGGAAGTTCTTCTGCAATTTCAGTGGTGATGAACGAGTGAGAAGCATGTTGTCGTTACTTCGGGCTTCAGAGATTTCATTTCCCGGAGAAAAAGTGATGGAAGAAGCCAAGGCATTCACAAGAGACTATCTAAACCAAGTTTTAGCTGGACTCGGGGATGTGACGGAC
GTGGATCAAAGCCTTTTGAGAGAGGTGAAGTACGCATTGGAGTTTCCATGGCATTGCAGTGTGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATATATTCACAAAATCAATCCTGGCTCAAGTCGGAAATCAATCAAAAATTTTTGGAATTAGCCAAATTGGACTTCAATATCCTGCAATGCATCTATCAGAAAGAGAC
ACAGTTTTTTTCAAGGTGGTGGAGCGACTCAGACATAGTGCAGCTGAATTTCTATAGGAAGCGTCACGTGGAATATTATTTTTGGGCGGTTATAGGCGTTCTCGAGCCCGAATTTTCTCAAAGCAGAATTGCCTTCGCAAAAATAGCTACCGTGGGAACTGTTCTAGATGACATCTGTGATACTCACGGAACTTTGGACG
AAGTCAGAACCATCACGGAGGCAGTCAGACGATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATACAGCGAATGAATTGGCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGAGGCTTATCTGCAA
GAAGCGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCGCTTCTGTTGATGGGTGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGATCAGACTCACAGA
CGACTTGAGAGATTTCGAGGAGGAGAAGGAACGCGGGGAGATAGCTTCAATTGTGGAGTGTTATATGAAAGAGAATCCTGAATCTACACTGGAAAATGCTTTAAATCACATAAAAGACATCCTTCATCAATCCCTAGAGGAATTGAATAGGGAGTTTATGAAGAAGGACAGTGTACCATTGTGTTGCAAGAAGTTCAGTT
TCAATATAGCACGAGGCATTCAATTCCTCTACAAATACGGAGACGGCATATGCATTTCTGACAAGGAAGTAAAGGAGCAGATAATCAAAATCCTCGTGGAGCAAGTGCCTATTGAGGAATACTGATGACCCGGTAGCTGTGTATGGCCATATATTAAATAAGTGGCGCATAATGAGACTTAGAAAGTTTGTAAAAGTGGC
CGTAGTTTGTCTAGATTTTGCGAACTTGCCGACGGACATTGTTTCCGCACAAATGACATATATCAAGTCAGGAC

Intron 5 GTTACGCATT ... AGCTGAGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	35.03	14	4	11
A2	34.56	19	11	9
A3	38.05	9.7	5	6.7
A4	48.57	9	13	4
A5	50	2	2	2
A6	20	4	1	1
A7	25	3	0	2
A8	0	1	0	0
A9	25.55	5.3	1	2.7
A10	60	1	0	3

>TRINITY_DN112767_c0_g1_i4 from sample A2 / Example spliced isoform

CGCTTCGCAGAGCGTAGTTTTATAATAAAGAGAGAGAGAGTTCAATTTGCACAAGTTATGGCCGACATCTCCGAATCTGTTGCCACCATTTTGAACGATGCATCTTCGATCGCTCGACGCACTGCCAATCATCACGGGAATGTCTGGGACGACGCTGTCATACAATCTCTCAATTCCCCCTATGGGGTATCATGGCAGCT
ATCTCTATCGTTGTCATCTGCTCTTTGTTTGCTTTTAGTTTTCAGTTTGTTTCAAGCGTTGGCATCTATGTTTCTTGAATTGCATTGCACTCTAATCAGGCACCTGCGTATCATGAACGCGTTGAAAAGCTTATTGTGGAGATCAAGCATTTACTTCTCACTGAAATGGAAGATACCAATCTTGATTTAATCAAACGTCT
TGAGATCGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACACGAAATTAAAACAGCTGCTCTCGATTACGTGTACAGATGTTGGAACGAAAGAGGTATCGGCGTGGGATCAAGAGATTCCCTCACCAAAGATCTTAACGCTACAGCTTTAGGATTTCGTGCTCTCCGACTTTATCGATATAACGTATCCG
CAGG

>TRINITY_DN112767_c0_g1_i12 from sample A2 / Example unspliced isoform:

CGCTTCGCAGAGCGTAGTTTTATAATAAAGAGAGAGAGAGTTCAATTTGCACAAGTTATGGCCGACATCTCCGAATCTGTTGCCACCATTTTGAACGATGCATCTTCGATCGCTCGACGCACTGCCAATCATCACGGGAATGTCTGGGACGACGCTGTCATACAATCTCTCAATTCCCCCTATGGGGCACCTGCGTATCA
TGAACGCGTTGAAAAGCTTATTGTGGAGATCAAGCATTTACTTCTCACTGAAATGGAAGATACCAATCTTGATTTAATCAAACGTCTTGAGATCGTTGACACATTGGAATGCCTTGGAATTGATCGACATTTTCAACACGAAATTAAAACAGCTGCTCTCGATTACGTGTACAGgttacgcatttctaaacacatgaagc
aatttaatttaagcgtttacatatatctgtacattttttttagtggtggtggggggtgtgttttattcgttcgttcgaagctgactgatgagctgagcagATGTTGGAACGAAAGAGGTATCGGCGTGGGATCAAGAGATTCCCTCACCAAAGATCTTAACGCTACAGCTTTAGGATTTCGTGCTCTCCGACTTTATCGA
TATAACGTATCCGCAGGAGTGTTGGAGAATTTCAAGAATGAGCACGGGAAGTTCTTCTGCAATTTCAGTGGTGATGAACGAGTGAGAAGCATGTTGTCGTTACTTCGGGCTTCAGAGATTTCATTTCCCGGAGAAAAAGTGATGGAAGAAGCCAAGGCATTCACAAGAGAATATCTAAACCAAGTTTTAGCTGGATTCGG
GGATGTGACGGACGTGGATCAAAGCCTTTTGAGAGAGGTGAAGTACGCATTGGAGTTTCCATGGCATTGCAGTGTGCCCAGATGGGAGGCAAGGAGCTTTATTGAAATCTATTCACAAAATCAATCCTGGCTCAAGTCGGAAATCAATCAAAAATTTTTGGAATTAGCCAAATTGGACTTCAATATCCTGCAATGCATCT
ATCAGAAAGAGACACAGTTTTTTTCAAGGTGGTGGAGCGACTCAGACATAGTGCAGCTGAATTTCTATAGGAAGCGTCACGTGGAATATTATTTTTGGGCGGTTATAGGCGTTCTCGAGCCCGAATTTTCTCAAAGCAGAATTGCCTTCGCAAAAATAGCTACCGTGGGAACTGTTCTAGATGACATCTGTGATACTCAC
GGAACTTTGGACGAAGTCAGAACCATCACGGAGGCAGTCAGACGATGGGATCTTTCGTTGTTAAAAGACCTTCCAGACAACATAAAAATTGCATTGCAATTCTTCTTCAATACAGCGAATGAATTGGCTGTTGAAGTCCTGAAAAAGCAAGAGCGGGATATGACGGCCATATTACAAGATTGCTGGAAGAGATTTCTCGA
GGCTTATCTGCAAGAAGCGGAATGGATAGCAACTGGGCGTGTGCCCACTTTTAATGAGTACATAAAAAATGCCCGCGCTAGCACAGGGATGTGTATTGCGAATTTGATTCCGCTTCTGTTGATGGGTGAGCTTCTCCCGGACAACATTCTGGAGCAAATACATTCTCCATCTAAGATCCAGGAGCTCTCAGAATTGACGA
TCAGACTCACAGACGACTTGAGAGATTTCGAGGAGGAGAAGGAACGCGGGGAGATAGCTTCAATTGTGGAGTGTTATATGAAAGAGAATCCTGAATCTACACTGGAAAATGCTTTAAATCACATAAAAGACATCCTTCATCAATCCCTAGAGGAATTGAATAGGGAGTTTATGAAGAAGGACAGTGTACCATTGTGTTGC
AAGAAGTTCAGTTTCAATATAGCACGAGGCATTCAATTCCTCTACAAATACGGAGACGGCATATGCATTTCTGACAAGGAAGTAAAGGAGCAGATAATCAAAATCCTCGTGGAGCAAGTGCCTATTGAGGAATACTGATGACCCGGTAGCTGTGTATGGCCATATATTAAATAAGTGGCGCATAATGAGACTTAGAAAGT
TTGTAAAAGTGGCCGTAGTTTGTCTAGATTTTGCGAACTTGCCGACGGACATTGTTTCCGCACAAATGACATATATCAAGTCAGGACCGATTTCCTTCAAG