sp|P45734|PALY_TRISU ----> Phenylalanine ammonia-lyase

Intron 1 GTAGGTATCT ... TTTCATATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	49.79	60	47	72
A2	73.86	23	30	100
A3	73.33	32	57	119
A4	60	40	47	73
A5	61.11	28	15	73
A6	41.94	36	15	37
A7	37.11	61	28	44
A8	37.06	45	16	37
A9	41.86	50	5	67
A10	28.67	51	10	31

>TRINITY_DN121606_c0_g1_i3 from sample A3 / Example spliced isoform

AAAATCTGGGGTCGAGAAGGCTTCCTAAATGTAATCTTCCAAGGCGTTGGAATGTTTGATTACAGAACCTGTGGAGAACGGACGAGGAGGAGATCAAGAGGCTCGGGCGCGTGGAGATCATCAGGGTTTCATCAATGGCCTGTCATAAACCCACCACTCTCTGACTTCGCCTATACTATTATTATGTATATGTGGAGAAT
GGTGACGGGGTTGGCGAGGTACTGGAGCCCCGAAATGTAATCGTAGCCAGCTCTTCCAAGGCGTTGAAATGTAGGATTACAGAACCGGTGGAGAACGGACGAAGAGGAGATCATCAGTTAGGATTTCTCTCTGACTTCGCCTATACTATTAATATGTATGGGTTAAAGTAGAATTGGGTTCGATTAATATGAAAATAGGT
TAAATTTTTAGATGAGTTTAAAG

>TRINITY_DN121606_c0_g1_i4 from sample A3 / Example unspliced isoform:

AAAATCTGGGGTCGAGAAGGCTTCCTAAATGTAATCTTCCAAGGCGTTGGAATGTTTGATTACAGAACCTGTGGAGAACGGACGAGGAGGAGATCAAGAGGCTCGGGCGCGTGGAGATCATCAGgtaggtatctctctgacttcgcctatactattattatgttcgatttatgtgaaattgggttcgattaaagtggaat
taggttaaaattttagatgaatttaaagtctcacgaaactttgatcagaaaggctattagttataagtttgtgctatttctatttcatatagGGTTTCATCAATGGCCTGGCATAAACCCACCAG

Intron 2 GTAGGGTTTC ... TTTCATATAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	41.48	358.7	100.3	254.3
A2	48.24	230.3	51.7	214.7
A3	44.91	281.7	71.3	229.7
A4	43.5	224.3	77.7	172.7
A5	55.27	103.3	29	127.7
A6	27.57	704	78	268
A7	33.17	1049	66	520.7
A8	29.01	992.7	77	405.7
A9	32.84	875.3	67.7	428
A10	29.17	858.7	74	353.7

>TRINITY_DN46169_c1_g4_i6 from sample A7 / Example spliced isoform

ATTGGGATGTCTGATTACAAAACCTGTGCAGAACGGGCGAGGAGGAGATCAAGAGGCTCGGGCGGGTGGAGATCATCAAGCCAGATCTTCCAAGGTATTGGGATGTCTGATTACAAAACCTGTGCAGAACGGACGAGGAGGAGATCAAGAGGTTCGGGCGGGTGGAGATCATCAGGGTTTCATCAATGGCCTGGCATAAA
CCCACCAGATATGTGGAGAACCTCATCTTTAAAATCTCTGGTTGTTTTGTTTGGCGGTCTGGACGTGAATGGTGACGGGGTTGGCGAGGTAATCGTAGCCAGATCTTCCAAGGCGTTGGAATGTCGGATTACAGAACCTATGGCGAACGGACAAGGAGGAGATCAAGAGGCTCGGGTGCGTGGAGATCATCACGGTTTCA
TCAATGGCCTGGCATAAACCCACCAGATATGTGGAGAACCTCATCTTTAAAATCCCTGGTTGATTTGTTCGGCGGTCTGGACGTGAATGGTGACGGGGTTGGCGAGGTAATCGTAGCCGGATCTTCCAAGGCGTTGGAATGTCGGATTACAGAACCTATGGCGAACGGACAAGGAGGAGATCAAGAGGCTCGGGTGCGTG
GAGATCATC

>TRINITY_DN46169_c1_g4_i7 from sample A7 / Example unspliced isoform:

ATGACTTGTAGGAGCTCCTTCTGGCAAAATCGGATGTCAAGAAGGCTAATCGTAGCCAGATCTTCCAAGGTGTTGGGATGCCTGATTACAAAACCTGTGCACGGACGAGGAGGAGATCAAGAGGCTCGGGCGGGTGGAGATCATCAGgtagggtttctctctgacttcgcctatactattattatgtctgggttaaagtt
gaattgggttcgattaaagtgaaattgggtttgattaaagtggaattaggttacaattttagatgagtttagagtctcacgaaactttgatcagaaaggctattagttataagtctgtgctatttatatttcatatagGGTTTCATCAATGGCCTGGCATAAACCCACCAGATATGTGGAGAACCTCATCTTTAAAATCT
CTGGTTGTTTTGTTTGGCGGTCTGGACGTGAATGGTGACGGGGTTGGCGAGGTAATCGTAGCCAGATCTTCCAAGGCGTTGGAATGTCGGATTACAGAACCTATGGCGAACGGACAAGGAGGAGATCAAGAGGCTCGGGTGCGTGGAGATCATCACGGTTTCATCAATGGCCTGGCATAAACCCACCAGATATGTGGAGA
ACCTCATCTTTAAAATCCCTGGTTGATTTGTTCGGCGGTCTGGACGTGAATGGTGACGGGGTTGGCGAGGTAATCGTAGCCGGATCTTCCAAGGCGTTGGAATGTCGGATTACAGAACCTATGGCGAACGGACAAGGAGGAGATCAAGAGGCTCGGGTGCGTGGAGATCATC