NA ----> NA

Intron 1 GTCGGTGATA ... GTGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	-1	0	0	0
A2	-1	0	0	0
A3	-1	0	0	0
A4	33.33	1	1	0
A5	-1	0	0	0
A6	33.33	1	1	0
A7	48.39	16	10	20
A8	38.1	13	12	4
A9	28.24	11	6	2.7
A10	25.93	10	3	4

>TRINITY_DN48561_c4_g2_i3 from sample A7 / Example spliced isoform

ACAGGTTTGATGATGAAGATGGATAAATTCATAACAGAGTACCAAAATTGTCGAGATTGTTCTTCCAAATATCAAAATAAATTGCAGGGTTTAAGGCCATGTTTTTATTTCTGCCAGATAATTGCCGATTGATTGCATATCATTTCCACGTTGCTAAACAGGTTGTTGAGACAATAAATTTTTGCCATCTTTCCAGGATT
GTTGCACTGGATTGTAGAGATCTGGGGTCTCACACATTTGTAATCTTTATTATGGAAAATGCATCAATGCAAAATAGTTACAGAGCAGTGCTATCTGTACGCAAAGATTCGTGTGCCACGAGAAAGAAGTTTATGCCACGTCTCCTGGGAAACATAGTTTTACCAAGTTTGATGGCAAAGCTGTGAGGATGAAAGAAAAT
TACCAGGTTAAAGTGGAGCAAAGTCATTGCGGTAGAAAATTTCACCAGAAAGGAAACCAACCCGTTAATATGGCAGGGAAGATGGCGGGCAGGTCGCACAGCGAGCAGAATTGAGAATTAATTTGGGGATTCAACACATCAAACAAGACCAGAAATAGTAGATTCGTTGCCTGAAATTAAGGGAGAAAACAAACTCCTGT
AGCTTATATAAAGCAAAGAATTCGATTGCAAAAGGAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTCCATTCTGTTTCTGCTTGTAAATCTTTATATTTGCAGATAGAAGGAGAGATTCTGCCAGAAGTGATTTCTGGTAATGTAAAGAAATCTGTGTTCTTCAATCCAGAAATATTTGCAACCAGA
GATTGCTGCTATTACTGTTCCAAGATTTT

>TRINITY_DN48561_c4_g2_i5 from sample A7 / Example unspliced isoform:

ACAGGTTTGATGATGAAGATGGATAAATTCATAACAGAGTACCAAAATTGTCGAGATTGTTCTTCCAAATATCAAAATAAATTGCAGGGTTTAAGGCCATGTTTTTATTTCTGCCAGATAATTGCCGATTGATTGCATATCATTTCCACGTTGCTAAACAGGTTGTTGAGACAATAAATTTTTGCCATCTTTCCAGGATT
GTTGCACTGGATTGTAGAGATCTGGGGTCTCACACATTTGTAATCTTTATTATGGAAAATGCATCAATGCAAAATAGTTACAGAGCAGTGCTATCTGTACGCAAAGATTCGTGTGCCACGAGAAAGAAGTTTATGCCACGTCTCCTGGGAAACATAGTTTTACCAAgtcggtgatagaattcatcatatttctatcgaag
gaattacagtagctcaatttgttttcgaattgcgtcaccaaatgtggacttcaactaagataaaatatcatatttcgtgtgtgcagGTTTGATGGCAAAGCTGTGAGGATGAAAGAAAATTACCAGGTTAAAGTGGAGCAAAGTCATTGCGGTAGAAAATTTCACCAGAAAGGAAACCAACCCGTTAATATGGCAGGGAA
GATGGCGGGCAGGTCGCACAGCGAGCAGAATTGAGAATTAATTTGGGGATTCAACACATCAAACAAGACCAGAAATAGTAGATTCGTTGCCTGAAATTAAGGGAGAAAACAAACTCCTGTAGCTTATATAAAGCAAAGAATTCGATTGCAAAAGGAGGGGGGGTTGATCTCAGCAGAATAGAGAGGACTTTGTAATTCTC
CATTCTGTTTCTGCTTGTAAATCTTTATATTTGCAGATAGAAGGAGAGATTCTGCCAGAAGTGATTTCTGGTAATGTAAAGAAATCTGTGTTCTTCAATCCAGAAATATTTGCAACCAGAGATTGCTGCTATTACTGTTCCAAGATTTT

Intron 2 GTTGTTGAGA ... GTGTGTGCAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	1	1	0
A2	63.64	4	7	0
A3	61.54	5	8	0
A4	50	1	1	0
A5	-1	0	0	0
A6	66.67	3	6	0
A7	65.63	11	21	20
A8	65.52	10	19	4
A9	59.52	17	25	2
A10	66	17	33	4

>TRINITY_DN46314_c1_g1_i2 from sample A8 / Example spliced isoform

TGAAGGTTTGATGATGAAGATGGATAAATTCATAACAGAGTACCAAAATTGTCGAGATTGTTCTTCCAAATATCAAAATAAATTGCAGGGTTTAAGGCCATGTTTTTATTTCTGCCAGATAATTGCCGATTGATTGCATATCATTTCCACGTTGCTAAACAGGTTTGATGGCAAAGCTGTGAGGATGAAAGAAAATTACC
AGGTCAAAGTGGAGCAAAGTCATTGCTGTAGAAAATTTCACCAGAAAGGAAACCAACCCGTTAATATGGCAGGGAAGATGGCGGGCAGGTCGCACAGCGAGCAGAATTGAGAATTAATTTGGGGATTCAACACATCAAACAAGACCAGAAATAGTAGATTCGTTGCCTGAAATTAAGGGAGAAAACAAACTCCTGTAGCT
TATATAAAGCAAAGAATTCGATTGCAAAAGGAGGGGGGGTTGATCTCAGCAGAATAGAGAGGA

>TRINITY_DN46314_c1_g1_i3 from sample A8 / Example unspliced isoform:

TGAAGGTTTGATGATGAAGATGGATAAATTCATAACAGAGTACCAAAATTGTCGAGATTGTTCTTCCAAATATCAAAATAAATTGCAGGGTTTAAGGCCATGTTTTTATTTCTGCCAGATAATTGCCGATTGATTGCATATCATTTCCACGTTGCTAAACAGgttgttgagacaataaatttttgccatctttccaggat
tgttgcactggattgtagagatctggggtctcacacatttgtaatctttattatggaaaatgcatcaatgcaaaatagttacagagcagtgctatctgtacgcaaagattcgtgtgccacgagaaagaagtttatgccacgtctcctgggaaacatagttttaccaagtcggtgatagaattcatcatatttctatcgaa
ggaattacagtagctcaatttgttttcgaattgcgtcaccaaatgtggacttcaactaagataaaatatcatatttcgtgtgtgcagGTTTGATGGCAAAGCTGTGAGGATGAAAGAAAATTACCAGGTCAAAGTGGAGCAAAGTCATTGCTGTAGAAAATTTCACCAGAAAGGAAACCAACCCGTTAATATGGCAGGGA
AGATGGCGGGCAGGTCGCACAGCGAGCAGAATTGAGAATTAATTTGGGGATTCAACACATCAAACAAGACCAGAAATAGTAGATTCGTTGCCTGAAATTAAGGGAGAAAACAAACTCCTGTAGCTTATATAAAGCAAAGAATTCGATTGCAAAAGGAGGGGGGGTTGATCTCAGCAGAATAGAGAGGA