sp|O22929|BL1S1_ARATH ----> Biogenesis of lysosome-related organelles complex 1 subunit 1

Intron 1 GTCTCTTTGT ... TATTTAAAAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	50	3	0	3
A2	46.15	14	2	12
A3	46.43	15	3	13
A4	54.29	16	9	19
A5	50	3	0	3
A6	50	67	17	67
A7	51.64	59	24	63
A8	52.83	75	25	84
A9	52.7	70	7	78
A10	50	47	17	47

>TRINITY_DN107632_c0_g1_i3 from sample A2 / Example spliced isoform

GGCGGGGAAGGGTCTTGTCTGTCTAAGGAGATTCGATCGCTAGACCTTTTTCAAGAGTGGAGCCAAGAAATGGCGAAATTGGAAGCGGCACTGATCCAAATGATGAGCGACCATTCTCATTCAAATGTTCAGCTCAGAGATCAACTAGGACACTCACCAGAGGACATCATTCAGCAGGCACTTGGTGTTGATGTCTGCAG
TGTGCACTACTGATTTGGATGACGAGTGGAGCTAGCTCCTCCCTCTCTTGTTGCTTGCCGCTCATCACTCGCTGCACCTCATTCTCAGGACTATGCGAAGAAGGATGCTTTAGCAACTGCAACCCGTGTTTCAGACCTGCTTGTCGATAGTGTGAATAGTGGGGTTCAAGAATCCTTTATTAATGAGAAACGCATTGAAC
ATGAAACTCGTGCATTGTCTGCAACAGTGCAGCGGTATATAAAGCAAACAAATCAATGGCTTGGAGTAGTCAATGCCTTGGATTCAGCTCTAAAGGAAATTGGAGATTTTGAGAACTGGATGAAGATTATGGAATATGACTGTGAGAGCATAAGCCTTGCATTGCATTACATCCACAAGAAAGAAAACCCACAGTTATGA
GCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACTGTAACGAGTCAATCGAGGCAGTGAAAGTATCTGATTTGGAGTATGTACAGTTGTGTTTAAATTCCATATGGAAGTATATTTAGGTTAATGTGCTCAAGTAGTTTGATTTTGTAGATTTTCCCAAAAATGATTGG
TAAGTACGAAACAGACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACGGTAACGAGTCAATCAAGGCAAGGAAAGTATCTGATTTTGAGTATGTACAGTTGTGTTTAAATTCCATATGGAAGTATATTTAGGCTAATGTGCTTAAGTAGTTTGATTTT
GTAGATTTTCCCAAAAATGATTGGTAAGTACGAAACAGACAGTTATGAGCAAGCTCATTTC

>TRINITY_DN107632_c0_g1_i1 from sample A2 / Example unspliced isoform:

GGCGGGGAAGGGTCTTGTCTGTCTAAGGAGATTCGATCGCTAGACCTTTTTCAAGAGTGGAGCCAAGAAATGGCGAAATTGGAAGCGGCACTGATCCAAATGATGAGCGACCATTCTCATTCAAATGTTCAGCTCAGAGATCAACTAGGACACTCACCAGAGGACATCATTCAGCAGGCACTTGGTGTTGATGTCTGCAG
TGTGCACTACTGATTTGGATGACGAGTGGAGCTAGCTCCTCCCTCTCTTGTTGCTTGCCGCTCATCACTCGCTGCACCTCATTCTCAGGACTATgtctctttgttgactcttcttcacctcctagggttcaccttcttggtttaactcgtttatttaaaagGCGAAGAAGGATGCTTTAGCAACTGCAACCCGTGTTTCA
GACCTGCTTGTCGATAGTGTGAATAGTGGGGTTCAAGAATCCTTTATTAATGAGAAACGCATTGAACATGAAACTCGTGCATTGTCTGCAACAGTGCAGCGGTATATAAAGCAAACAAATCAATGGCTTGGAGTAGTCAATGCCTTGGATTCAGCTCTAAAGGAAATTGGAGATTTTGAGAACTGGATGAAGATTATGGA
ATATGACTGTGAGAGCATAAGCCTTGCATTGCATTACATCCACAAGAAAGAAAACCCACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACTGTAACGAGTCAATCGAGGCAGTGAAAGTATCTGATTTGGAGTATGTACAGTTGTGTTTAAATTCCAT
ATGGAAGTATATTTAGGTTAATGTGCTCAAGTAGTTTGATTTTGTAGATTTTCCCAAAAATGATTGGTAAGTACGAAACAGACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACGGTAACGAGTCAATCAAGGCAAGGAAAGTATCTGATTTTGAGTA
TGTACAGTTGTGTTTAAATTCCATATGGAAGTATATTTAGGCTAATGTGCTTAAGTAGTTTGATTTTGTAGATTTTCCCAAAAATGATTGGTAAGTACGAAACAGACAGTTATGAGCAAGCTCATTTC

Intron 2 GTGACACACT ... TGTATTGTAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	15.66	70	0	13
A2	19.05	51	0	12
A3	20.48	66	0	17
A4	13.21	46	0	7
A5	25.71	26	0	9
A6	17.2	515	0	107
A7	18.01	305	0	67
A8	16.46	446.5	0	88
A9	21.93	445	1	125
A10	14.37	292	2	49

>TRINITY_DN41390_c0_g1_i5 from sample A7 / Example spliced isoform

GAAGGGTCTTGTCTGTCTAAGGAGATTCGATCGCTAGACCTTTGTCAAGAGTGGAGCCAAGAAATGGCGAAATTGGAAGCGGCACTGATCCAAATGATGAGCGACCATTCTCATTCAAATGTTCAGCTCAGAGATCAACTAGAAAAGGCGAAGAAGGATGCTTTAGCAACTGCAACCCGTGTTTCAGACCTGCTTGTCGA
TAGTGTGAATAGTGGGGTTCAAGAATCCTTTATTAATGAGAAACGCATTGAACATGAAACTCGTGCATTGTCTGCAACAGTGCAGCGGTATATAAAGCAAACAAATCAATGGCTTGGAGTAGTCAATGCCTTGGATTCAGCTCTAAAGGAAATTGGAGATTTTGAGAACTGGATGAAGATTATGGAATATGACTGTGAGA
GCATAAGCCTTGCATTGCATTACATCCACAAGAAAGAAAACCCACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACTGTAACGAGTCAATCGAGGCAGTGAAAGTATCTGATTTGGAGTATGTACAGTTGTGTTTAAATTCCATATGGAAGTATATTT
AGGTTAATGTGCTCAAGTAGTTTGATTTTGTAGATTTTCCCAAAAATGATTGGTAAGTACGAAACAGACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACGGTAACGAGTCAATCAAGGCAGGGAAAGTATCTGATTTTGAGTATGTACAGTTGTGTT
TAAATTCCATATGGAAGTATATTTAGGCTAATGTGCTTAAGTAGTTTGATTTTGTAGATTTTCCAAAAAAAGATTGGTGAGTATGAAACAGAGGATTTGTTAACCTCATTGTATTTATATTTTGACATTATAGAAATTCCTGGGATTATGGGGC

>TRINITY_DN41390_c0_g1_i6 from sample A7 / Example unspliced isoform:

TGCCTTGGATTCAGCTCTAAAGgtgacacactctccttttagtattagttattgtttagaagtttctgaacgcatgggagtttgatggcatgttatttacttaattgcaaatcaatgtactgttcacgtgaagatctagtctttgtgatgctaatataagtattatttatgttgtaacatcagtactgagtcataattag
tgatgattgcttgggtaactgactggattttgatattttggctacgttggactgtttacatgtaagattcacatgatgtcttatgcataagattaccaccgttgtggggggtggggtttggcatgcacaagcaaccttagtatttgtcaagttctaaatgctaaatttgactgtgctttgcaagtgtattgtagGAAATT

GGAGATTTTGAGAACTGGATGAAGATTATGGAATATGACTGTGAGAGCATAAGCCTTGCATTGCATTACATCCACAAGAAAGAAAACCCACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACTGTAACGAGTCAATCGAGGCAGTGAAAGTATCTGAT
TTGGAGTATGTACAGTTGTGTTTAAATTCCATATGGAAGTATATTTAGGTTAATGTGCTCAAGTAGTTTGATTTTGTAGATTTTCCCAAAAATGATTGGTAAGTACGAAACAGACAGTTATGAGCAAGCTCATTTCATCTTGTCCGATCTACCTCTTTTTATCATCTCTTGATTTGCACAATTTGAACGGTAACGAGTCA
ATCAAGGCAGGGAAAGTATCTGATTTTGAGTATGTACAGTTGTGTTTAAATTCCATATGGAAGTATATTTAGGCTAATGTGCTTAAGTAGTTTGATTTTGTAGATTTTCCAAAAAAAGATTGGTGAGTATGAAACAGAGGATTTGTTAACCTCATTGTATTTATATTTTGACATTATAGAAATTCCTGGGATTATGGGGC