NA ----> NA

Intron 1 GTAAGTGATG ... TCTTGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	44.44	10	5	8
A2	50	4	0	4
A3	42.11	11	3	8
A4	0	6	5	0
A5	0	5	0	0
A6	33.8	70.5	7	36
A7	33.95	107	8	55
A8	32.69	70	8	34
A9	34.37	116.5	29	61
A10	33.99	101	7	52

>TRINITY_DN44449_c1_g1_i3 from sample A8 / Example spliced isoform

CAAATAACTCGTGAAACATGAATGCCAATCTCCCATCCTATTCATTGAATTCCAAATACTTAAAGGCGAATGCGTCATGCTTATGAATTCCAAATATTTAACGCGAATGCTTCATGCTTATGAATTCCAAATATTTAAGGCGGATTACTCTGCCTTTCTAGATCTCTTAAATTTTTGTTTCATCGTGGACTGATTCTGTA
GCTGGCGAATCATCTAATGCGCAGGAAATTAATATCAGATTGTGTGTTTTCTTGCACAATAACAGTTATGTGTTTTCTTGCACAGTAAATAAGCCTTTAATCATCAAGTGCGGCGGTCAAGACCTTAGTTCAGCTTAAGGTCTTTTAATATTTGAAGAAGGGGTGGAAAGAAGTAGAGGGATTGAGCAGCATCAAGATGC
CTC

>TRINITY_DN44449_c1_g1_i4 from sample A8 / Example unspliced isoform:

CAAATAACTCGTGAAACATGAATGCCAATCTCCCATCCTATTCATTGAATTCCAAATACTTAAAGGCGAATGCGTCATGCTTATGAATTCCAAATATTTAACGCGAATGCTTCATGCTTATGAATTCCAAATATTTAAGGCGGATTACTCTGCCTTTCTGGATCTCTTAAATTTGTGTTTCATCGTGGACTGATTCTGTA
GCTGGCGAATCATCTAATGCGCAGgtaagtgatgacttatctgttctctctgctatgacttatattccttttattattgttgtgctgctgcaaacatatcaacgttttattggacgagcagcttatgtgaaaagagttcaaaacattcaagcttattttggatactttttgaataatctttgatatgaaaggaagagtag
ggtatagtattagtcctggtaaccagccagcatttgaatagataagatttattctgagattcggcagaatttttaatatcagattatctgttttcttgcacagGAAATTAATATCAGATTGTGTGTTTTCTTGCACAATAACAGTTATGTGTTTTCTTGCACAGTAAATAAGCCTTTAATCATCAAGTGCGGCGGTCAAG
ACCTTAGTTCAGCTTAAGGTCTTTTAATATTTGAAGAAGGGGTGGAAAGAAGTAGAGGGATTGAGCAGCATCAAGATGCCTC

Intron 2 GTAAGTGATG ... TCTTGCACAG

Sample	Retention score (%)	Exon junction reads	5'ss reads	3'ss reads
A1	60	4	5	6
A2	33.33	10	0	5
A3	40	9	3	6
A4	0	10	5	0
A5	33.33	2	0	1
A6	49.54	55	7	54
A7	58.05	73	8	101
A8	39.34	74	8	48
A9	41.75	120	29	86
A10	53.57	91	7	105

>TRINITY_DN44449_c1_g1_i10 from sample A8 / Example spliced isoform

CAAATAACTCGTGAAACATGAATGCCAATCTCCCATCCTATTCATTGAATTCCAAATACTTAAAGGCGAATGCGTCATGCTTATGAATTCCAAATATTTAACGCGAATGCTTCATGCTTATGAATTCCAAATATTTAAGGCGGATTACTCTGCCTTTCTGGATCTCTTAAATTTGTGTTTCATCGTGGACTGATTCTGTA
GCTGGCGAATCATCTAATGCGCAGTAAATAAGCCTTTAATCATCAAGTGCGGCGGTCAAGACCTTGGTTCAGTTTAAGGTCTTTTAATATTTGAAGAAGGGGTGGAAAGAAGTAGAGGGATTGAGCAGCATCAAGATGCCTCCATTCTCAAAAAGATGTACCGATCATCTGAATAATAACGTTGGGACATACTGTTGCTT
AATGCTGACCGCTTTAGCTGCT

>TRINITY_DN44449_c1_g1_i4 from sample A8 / Example unspliced isoform:

CAAATAACTCGTGAAACATGAATGCCAATCTCCCATCCTATTCATTGAATTCCAAATACTTAAAGGCGAATGCGTCATGCTTATGAATTCCAAATATTTAACGCGAATGCTTCATGCTTATGAATTCCAAATATTTAAGGCGGATTACTCTGCCTTTCTGGATCTCTTAAATTTGTGTTTCATCGTGGACTGATTCTGTA
GCTGGCGAATCATCTAATGCGCAGgtaagtgatgacttatctgttctctctgctatgacttatattccttttattattgttgtgctgctgcaaacatatcaacgttttattggacgagcagcttatgtgaaaagagttcaaaacattcaagcttattttggatactttttgaataatctttgatatgaaaggaagagtag
ggtatagtattagtcctggtaaccagccagcatttgaatagataagatttattctgagattcggcagaatttttaatatcagattatctgttttcttgcacaggaaattaatatcagattgtgtgttttcttgcacaataacagttatgtgttttcttgcacagTAAATAAGCCTTTAATCATCAAGTGCGGCGGTCAAG
ACCTTAGTTCAGCTTAAGGTCTTTTAATATTTGAAGAAGGGGTGGAAAGAAGTAGAGGGATTGAGCAGCATCAAGATGCCTC